• Korean Journal of Environmental Agriculture
• /
• v.22 no.1
• /
• pp.36-40
• /
• 2003

Cyclosulfamuron a herbicide of sulfonylurea type, is a relatively new compound which control broad leaves and perennial weeds in rice field. However, this herbicide has a minor disadvantage of decreasing rice plant growth, especially in early growth stage. Therefore, far introducing this cyclosulfamuron as a herbicide in rice field, it is important to minimize the suppression of early plant growth with maintaining weed control efficacy. This study was conducted to evaluate effects of cyclosulfamuron early plant growth and acetolactate synthase activity of rice (Oryza sativa cv Dongjinbyeo, Hwasungbyeo, Ilpumbyeo). Rice growth was inhibited by cyclosulfamuron in their early growth stage. The concentrations required far 50% inhibition of Dongjinbyeo, Hwasungbyeo and Ilpumbyeo growth were 6.3, 9.2 and 146.mg/kg, respectively. Inhibition effect of cyclosulfamuron on the root elongation was greater than the effect on the shoot growth. Concentrations required far 50% inhibition of acetolactate synthase activity from Dongjinbyeo, Hwasungbyeo, Ilpumbyeo were 42.7, 32.7 and $56.7\;{\mu}M$, respectively.

• Journal of the Korean Applied Science and Technology
• /
• v.8 no.1
• /
• pp.35-43
• /
• 1991

Dried seeds of Camellia japonica and Thea sinensis were investigated to determine the nature of their antioxidative activity. Activity was measured by the induction period in the coupled oxidation of a substrate lard and extracts or isolates to be tested. 70% methanol and dichloromethane extracts were found to be antioxidative abilities. Their unsaponifiables revealed weak antioxidative activity, although hexane extracts did not show antioxidative effect on lard. Column chromatography for dlchloromethane extracts gave 4 fractions(only 2 fractions were potent). HPLC was used in isolating potent antioxidative components from the column fractions and the precolumn-passed methanol extracts. They were separated into 7 and 8 components, respectively. The column fractions obtained from both seeds comprised trans-p-coumaric acid. trans-p-ferulic acid and an unknown component with minor components such as chlorogenic acid and catechin. On the other hand, the most prominent components in the methanol extracts were an unidentified component. trans-pcoumaric acid, trans-p-ferulic acid, catechin and chlorogenic acid. The unknown compound isolated from the column fractions and methanol extracts was identified as epicatechin by $^1H-and\;^{13}C-NMR$. The antioxidative activities of these components were epicatechin > catechin > chlorogenic acid > trans-p-ferulic acid > trans-p-coumaric acid.

• Journal of Food Hygiene and Safety
• /
• v.2 no.1
• /
• pp.9-14
• /
• 1987

With the market of food products, the use of food additives is on the increase. The dye as food additives, can be used for some foods which are difficult to preserve their own colors. It can be also classified as tar dye, vegetable dye and mineral dye. Because tar dye has dense toxicity, only 15 articles among them are legally allowed to be used. Among the allowed articles, the azo compound amaranth, tartrazine, sunset yellow, and allura red, were used in determining and comparing rat hepatic azo reductase activity and we observed the flavin's effects as follows: 1. Investigation with amaranth as substrate gave an apparent Km of $645\;\mu\textrm{M}$ and Vmax of 50 n mol/min/mg protein. 2. On investigation using a fixed amaranth concentration over a range of flavin concentration, FAD significantly increased the activity of the azo reductase compared with only minor increases in reaction mediated by the NADPH-generating system alone. 3. On investigation with amaranth, tartrazine, sunset yellow allura red as electron acceptor in the absence or presence of 300 mM-FAD, sunset yellow was reduced at a rat similar to amaranth, tartrazine was reduced at a slower rate and allura red was reduced a little more rapidly.

• Natural Product Sciences
• /
• v.15 no.4
• /
• pp.203-207
• /
• 2009

A homoisoflavanone, 7,4'-dihydroxyhomoisoflavanone (1) and a flavanone, (2S)-7,4'-dihydroxy-5-methoxyflavanone (2), were isolated from the rhizomes of Anemarrhena asphodeloides, together with 4,4'-dihydroxychalcon (3), 2'-O-methylphlorethin (4), 1,3-bis-di-p-hydroxyphenyl-4-penten-1-one (5), and 2,4'-dihydroxy-4-methoxybenzophenone (6) on the basis of spectroscopic and physicochemical analyses including 1Dand 2D- NMR techniques as well as by comparison of their data with the published values. Compounds 1 - 4 were isolated for the first time from this plant source. Among isolates, compound 2 exhibited moderate inhibitory effect on the differentiation of pre-adipocyte 3T3-L1 cells.

• Journal of Ginseng Research
• /
• v.39 no.4
• /
• pp.392-397
• /
• 2015

Background: Red ginseng (RG) is processed from Panax ginseng via several methods including heat treatment, mild acid hydrolysis, and microbial conversion to transform the major ginsenosides into minor ginsenosides, which have greater pharmaceutical activities. During the fermentation process using microbial strains in a machine for making red ginseng, a change of composition occurs after heating. Therefore, we confirmed that fermentation had occurred using only microbial strains and evaluated the changes in the ginsenosides and their chemical composition. Methods: To confirm the fermentation by microbial strains, the fermented red ginseng was made with microbial strains (w-FRG) or without microbial strains (n-FRG), and the fermentation process was performed to tertiary fermentation. The changes in the ginsenoside composition of the self-manufactured FRG using the machine were evaluated using HPLC, and the 20 ginsenosides were analyzed. Additionally, we investigated changes of the reducing sugar and polyphenol contents during fermentation process. Results: In the fermentation process, ginsenosides Re, Rg1, and Rb1 decreased but ginsenosides Rh1, F2, Rg3, and Compound Y (C.Y) increased in primary FRG more than in the raw ginseng and RG. The content of phenolic compounds was high in FRG and the highest in the tertiary w-FRG. Moreover, the reducing sugar content was approximately three times higher in the tertiary w-FRG than in the other n-FRG. Conclusion: As the results indicate, we confirmed the changes in the ginsenoside content and the role of microbial strains in the fermentation process.

• Bulletin of the Korean Chemical Society
• /
• v.8 no.2
• /
• pp.96-101
• /
• 1987

2-Ethoxy-6-methoxy-5-cyano-3,4-dihydro-2H-pyran (1_a$), 2-n-butoxy-6-methoxy-5-cyano-3,4-dihydro-2H-pyr an (1b), 2-isobutoxy-6-methoxy-5-cyano-3,4-dihydro-2H-py ran ($1_c$), and 2-ethoxy-6-methoxy-3-methyl-5-cyano-3,4-dihydro -2H-pyran ($1_d$) were prepared by (4 + 2) cycloaddition reaction of methyl $\alpha$-cyanoacrylate with the corresponding alkyl vinyl ethers. Compounds $1_{a-d}$ were ring-open polymerized by cationic catalyst to obtain alternating head-to-head (H-H) copolymers. For comparison, head-to-tail (H-T) copolymer $3_a$ was also prepared by free radical copolymerization of the corresponding monomers. The H-H copolymer exhibited minor differences in its $1_H% NMR and IR spectra, but in the $^{13}C$ NMR spectra significant differences were observed between the H-H and H-T copolymers. Glass transition temperature ($T_g$) of H-H copolymer was higher than that of the H-T copolymer, but thermal decomposition temperature of the H-H copolymer was lower than that of the H-T copolymer. Compounds $1_a$, $a_b$, and $1_c$, copolymerized well with styrene by cationic catalyst, but compound 1d failed to copolymerize with styrene. All of the H-H and H-T copolymers were soluble in common solvents and the inherent viscosities were in the range 0.2-0.4 dl/g.

• Journal of Dairy Science and Biotechnology
• /
• v.34 no.2
• /
• pp.117-135
• /
• 2016

The present study was performed to develop a functional raw food material from hydrolyzed whey protein powder (23%-GNANA) medication containing sialic acid as a marker compound that is naturally occurring at 7% concentration in GMP (glycomacropeptide). GMP is used worldwide in foodstuffs for babies and infants and is obtained from the milk protein as safe food. While the purpose of our detailed evaluation was aimed to assess preliminary NOAEL values for and above 2,000 mg/kg/day, a clinical dose allowance for 23%-GNANA (as per characteristic of a functional health product, a highly refined test substance of 23% (v/v) sialic acid combined in GMP), at the same time we also wanted to assess the safety of GMP hydrolyzate lacking sialic acid but with identical properties as GMP. Animal safety evaluation was conducted using 23%-GNANA as the test substance, produced from hydrolyzed whey protein powder (product name: HELICOBACTROL-23; provided by Medinutrol Inc. [Korea]; composed of 23% sialic acid and GMP protein) after isolating the sialic acid using enzymes approved as food additives, with GMP as a raw material, and subsequently increasing the content of xx up to 23% through 80% (v/v) ethanol soaking and concentrating, in accordance with GLP Guideline. The animal safety evaluation mentioned above was made on the basis of toxicity in SPF Sprague-Dawley female and male rats dosed with 10 mL of the test substance diluted to 0, 1,250, 2,500, and 5,000 mg/kg directly into their stomachs for 90 d. This was determined in terms of the general symptoms and animal viability, weight and amount of feed intake, eye examination, uracrasia tests, hematological and blood biochemical disorder tests, blood coagulation test, abnormal intestine weight, abnormalities during postmortem and histopathological examinations. Statistical significance was set at P<0.05. Based on the toxicity determination, a certain minor effect associated with the test substance was observed in male rats with no major effects of the tested substance, in comparison with the control group dosed with sterilized water. Nevertheless, the NOAEL value, evaluated as per toxicity criteria, was verified as 5,000 mg/kg/day (P<0.05). Similarly, for female rats, a certain minor effect associated with the test substance was observed in 5,000 mg/kg/day dosed group, with no major effect, yet the NOAEL value (as assessed as per toxicity criteria) was determined to be 5,000 mg/kg/day (P<0.05), which was the same as for male rats. Accordingly, the NOAEL values of the test substances for all female and male rats were finally verified as 5,000 mg/kg/day (P<0.05). In conclusion, it was determined that the 23%-GNANA test substance exceeds 2,000 mg/kg/day, the clinical allowance characteristic for functional health food, and was finally evaluated to cause no safety concerns when used as a raw material in functional health food production, which was the ultimate goal of the present study.

• Korean Journal of Food Science and Technology
• /
• v.40 no.6
• /
• pp.707-711
• /
• 2008

The principal objective of the current study was to isolate a purpurogallin derivative as an oxidation product from gallic acid, in an effort to assess the anti-inflammatory effects of this compound. Purpurogallin derivative is known to be the one of the oxidation products of gallic acid. This compound has been identified as a minor chemical component in fermented tea products. It has been previously demonstrated that theaflavins, the oxidation products of catechins found in fermented tea products, exert profound antioxidant and anti-inflammatory effects. However, the biological activities of a minor chemical component in fermented teas have yet to be evaluated. Purpurogallin carboxylic acid (PCA) was identified as a major oxidation product of gallic acid from a peroxidase/hydrogen peroxide oxidation model system. The identity of the PCA was verified by $^{1}H$ NMR, $^{13}C$ NMR and MS techniques. PCA treatment significantly suppressed the generation of pro-inflammatory mediators including nitric oxide and IL-6 in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. According to the nitrite assay, PCA 100, 75, and $50{\mu}g/mL$ treatment dose-dependently inhibited NO production by 57.6, 41.5, and 21.8%, respectively, in LPS-stimulated RAW264.7 murine macrophage cells. Moreover, IL-6 production was inhibited to a significant degree with PCA treatment of 100 and $75{\mu}g/mL$ at 43.1 and 23.9%, respectively. PCA treatment also significantly suppressed $PGE_2$ production at levels of 100 and $75{\mu}g/mL$. These results showed that PCA exerts inhibitory effects on the production of inflammatory mediators.

• Korean Journal of Soil Science and Fertilizer
• /
• v.8 no.2
• /
• pp.97-103
• /
• 1975

In order to use the glutamic acid fermentation residuum in agriculture effectively, comparison experiments of the residua liquid and dried solid, and compost on radish (Raphanus Sativus) and chinese cabbage (Brassica chinesis) were carried out, together with an investigation on some physical property changes of soil brought about by the use of the residua and compost. 1. An equal or somewhat superior effect of the glutamic acid fermentation residua solid and liquid, to urea was observed. 2. Both residua liquid and dried solid, tended to acidify soil and the tendency was observed to be somewhat servere in the latter. 3. The liquid fermentation residuum compounded with minor elements such as iron, manganese, zinc, copper and boron increased the yield of chinese cabbage compared with the liquid residuum alone. 4. Not only as a N, K fertilizer, the liquid residuum of glutamic acid fermentation could also be used as a source for liquid or solid compound fertilizer.

• Journal of Korean Society for Atmospheric Environment
• /
• v.21 no.2
• /
• pp.191-204
• /
• 2005

Building and furnishing materials and consumer product are important sources of volatile organic compounds(VOCs) and other aldehydes in the indoor environment. Some available evidence indicates that VOCs can cause adverse health effects to the building occupants and contribute to some of the symptoms of what we call, 'Sick House Syndrome' in Korea. The aims of this study were to evaluate the efficiency of emission system and to investigate comparison of the emission characteristics of different building materials such as wall-papers, paints, and adhesives. The emission of VOCs from building materials were determined in the small chambers defining the temperature, relative humidity, and ventilation rate in this study. VOCs were sampled for 20 minutes using Tenax-TA tubes and analysed by GC-MS with thermal desorption. The stability of conditions for temperature and relative humidity in this small chamber system showed that the fluctuation of temperature was between 25.4$\pm$0.3$^{\circ}C$ and that of relative humidity was 50.2$\pm$0.6$\%$ under the airflow rate of 167 mL/min. The emission tests from building materials resulted in TVOC emission rates of 0.011 $\~$ 3.108 mg/m$^{2}$h after 7 days. The general wall-papers emitted toluene abundantly and the natural wall-papers mainly emitted n-butanol and a minor amount of alkanes compound such as n -tetradecane. The remainder consisted of toluene, m,p -xylene, and styrene. The paints mainly emitted toluene and the adhesives mainly emitted chloroform as well as toluene. As a result, this study is expected to suggest meaningful data for future studies in exposure control through selecting healthy building materials and for the establishment of guidelines for various building materials in Korea.