• BMB Reports
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• 제43권10호
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• pp.698-703
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• 2010

In this study, we characterized two blocks of minisatellites in the 5' upstream region of the BORIS gene (BORIS-MS1, -MS2). BORIS-MS2 was found to be polymorphic; therefore, this locus could be useful as a marker for DNA fingerprinting. We assessed the association between BORIS-MS2 and breast cancer by a case-control study with 428 controls and 793 breast cancers cases. Rare alleles in the younger group (age, <40) were associated with a statistically significant increased risk of breast cancer (odds ratio, 4.84; 95% confidence interval, 1.06-22.22; and P = 0.026). A statistically significant association between the short rare alleles and cancer was identified in the younger group (8.02; 1.01-63.83; P = 0.021). Kaplan-Meier estimates showed that poor prognosis was associated with patients who contained the rare alleles. Our data suggest that the short rare alleles of BORIS-MS2 could be used to identify the risk for breast cancer in young patients.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.115.2-115.2
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• 2007

See Full Text

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.116.1-116.1
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• 2007

See Full Text

• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.1-4
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• 1999

Beijing White Chickens laying larger eggs and smaller eggs were respectively used as parental individuals for mating to produce the F1 progeny and then the F1 progeny individuals mated to produce 125 individuals of the F2 progeny. Three bands associated with the egg weight performance were identified from DNA fingerprints of the 125 individuals generated with a bovine minisatellite probe BM6.5B. The simple linear correlation analysis showed that the coefficients of correlation between frequencies of the three bands (DB1, DB2 and DB3) and egg weights were -0.6, -0.6 and 0.9, respectively.

• Journal of Astronomy and Space Sciences
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• 제24권4호
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• pp.297-308
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• 2007

2010년에 발사될 예정인 아리랑위성 5호의 부 탑재체인 AOPOD(Atmosphere Occultation and Precision Orbit Determination) 시스템은 GPS(Global Positioning System) 전파 엄폐(radio occultation) 자료를 제공한다. 이 논문에서는 아리랑위성 5호 궤도에서 발생하는 GPS 전파 엄폐의 발생 빈도 및 분포를 시뮬레이션하고, 현재 천문연구원에서 개발 중인 GPS 전파 엄폐 자료처리시스템인 KROPS(KASI Radio Occultation Processing System)를 사용한 전리층 전자밀도 산출결과를 제시하였다. 전자밀도를 산출하기 위해 2004년 6월 20일에 발생한 CHAMP(CHAllenging Minisatellite Payload) 위성의 GPS 전파 엄폐 관측값을 사용하였고 산출된 결과는 IRI(International Reference Ionosphere) - 2001 모델과 CHAMP 위성의 랑뮈어 탐침기(Planar Langmuir Probe) 및 이온존데 간과 비교하였다. 산출된 전자밀도를 이온존데 값과 비교했을 때, $F_2$층 최대전자밀도 고도인 $hmF_2$에서 약 5km, $F_2$층 최대전자밀도인 $NmF_2$에서 약 $3{\times}10^{10}el/m^3$의 차이를 보였으며, 랑뮈어 탐침기 값과 비교하여 고도 365.6km에서 두 값 모두 $1.6{\times}10^{11}el/m^3$로 일치하였다.

• Tuberculosis and Respiratory Diseases
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• 제76권2호
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• pp.59-65
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• 2014

Background: Variable-number tandem repeat (VNTR) typing is a promising method to discriminate the Mycobacterium tuberculosis isolates in molecular epidemiology. The purpose of this study is to determine the optimal VNTR combinations for discriminating isolated M. tuberculosis strains in Korea. Methods: A total of 317 clinical isolates collected throughout Korea were genotyped by using the IS6110 restriction fragment length polymorphism (RFLP), and then analysed for the number of VNTR copies from 32 VNTR loci. Results: The results of discriminatory power according to diverse combinations were as follows: 25 clusters in 83 strains were yielded from the internationally standardized 15 VNTR loci (Hunter-Gaston discriminatory index [HGDI], 0.9958), 25 clusters in 65 strains by using IS6110 RFLP (HGDI, 0.9977), 14 clusters in 32 strains in 12 hyper-variable VNTR loci (HGDI, 0.9995), 6 clusters in 13 strains in 32 VNTR loci (HDGI, 0.9998), and 7 clusters in 14 strains of both the 12 hyper-variable VNTR and IS6110 RFLP (HDGI, 0.9999). Conclusion: The combination of 12 hyper-variable VNTR typing can be an effective tool for genotyping Korean M. tuberculosis isolates where the Beijing strains are predominant.

• Journal of Astronomy and Space Sciences
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• 제36권3호
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• pp.133-147
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• 2019

Challenging Minisatellite Payload (CHAMP) satellite magnetic data are used to investigate the latitudinal variation of the storm-time meso-scale field-aligned currents by defining a new metric called the FAC range. Three major geomagnetic storm events are considered. Alongside SymH, the possible contributions from solar wind dynamic pressure and interplanetary magnetic field (IMF) $B_Z$ are also investigated. The results show that the new metric predicts the latitudinal variation of FACs better than previous studies. As expected, the equatorward expansion and poleward retreat are observed during the storm main phase and recovery phase respectively. The equatorward shift is prominent on the northern duskside, at ${\sim}58^{\circ}$ coinciding with the minimum SymH and dayside at ${\sim}59^{\circ}$ compared to dawnside and nightside respectively. The latitudinal shift of FAC range is better correlated to IMF $B_Z$ in northern hemisphere dusk-dawn magnetic local time (MLT) sectors than in southern hemisphere. The FAC range latitudinal shifts responds better to dynamic pressure in the duskside northern hemisphere and dawnside southern hemisphere than in southern hemisphere dusk sector and northern hemisphere dawn sector respectively. FAC range exhibits a good correlation with dynamic pressure in the dayside (nightside) southern (northern) hemispheres depicting possible electrodynamic similarity at day-night MLT sectors in the opposite hemispheres.

• 미생물학회지
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• 제40권2호
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• pp.65-72
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• 2004

진핵생물의 특정 염기배열을 원핵생물 내에서 증폭시킬 때 불안정성이 비교적 빈번히 관찰되어진다. 특히 long inverted repeats나 AT-rich sequences그리고 Z-DNA와 같은 구조를 지닌 염기배열은 대장균 내에서 매우 불안정하다. 이러한 염기서열은 대장균 내에서 부분적으로 결실되거나 완전히 손실된다. 본 연구실에서 human SCKI 유전자에 존재하는 몇 개의 tandem repeat (TR)에 대하여 다형성을 조사하였을 때, 어떤 TR 부분은 플라스미드로부터 빈번히 결실되어 그에 대한 염기서열 결정이 어려웠다. 그 결과 이러한 부분은 클론닝 될 수 없는 염기서열로 남게 되었다. 본 연구에서는 클론닝이 어려운 두 개의 TR 영역을 저온에서 클론닝하고 nebulizer나 sonicator를 이용하여 두 개의 library를 만들어 DNA 염기서열을 결정하였다. 이러한 연구는 복잡한 고등생물의 게놈연구에서 불안정한 게놈부분의 염기서열을 결정하는데 도움을 줄 것으로 사료된다.

• 천문학회보
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• 제37권2호
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• pp.125.1-125.1
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• 2012

The wave number 4 (wave-4) and wave number 3 (wave-3) longitudinal structures in the thermospheric neutral mass density are understood as tidal structures driven by diurnal eastward-propagating zonal wave number 3 (DE3) and wave number 2 (DE2) tides, respectively. However, those structures have been identified using data from limited time periods, and the consistency and recurrence of those structures have not yet been examined using long-term observation data. We examine the persistence of those structures by analyzing the neutral mass density data for the years 2001-2008 taken by the CHAllenging Minisatellite Payload (CHAMP) satellite. During years of low solar activity, the amplitude of the wave-4 structure is pronounced during August and September, and the wave-4 phase shows a consistent eastward phase progression of $90^{\circ}$ within 24 h local time in different months and years. During years of high solar activity, the wave-4 amplitude is small and does not show a distinctive annual pattern, but the tendency of the eastward phase shift at a rate of $90^{\circ}$/24 h exists. Thus the DE3 signature in the wave-4 structure is considered as a persistent feature. The wave-3 structure is a weak feature in most months and years. The amplitude and phase of the wave-3 structure do not show a notable solar cycle dependence. Among the contributing tidal modes to the wave-3 structure, the DE2 amplitude is most pronounced. This result may suggest that the DE2 signature, although it is a weak signature, is a perceivable persistent feature in the thermosphere.

• 약학회지
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• 제54권1호
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• pp.49-54
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• 2010

SLC6A19 which reported as a neurotransmitter was composed of seven minisatellites. In previous our study, the minisatellites variants of SLC6A19-MS7 showed the susceptibility for hypertension. When this minisatellte sequences were analyzed using the bioinformatic tool, USF1 (upstream transcription factor 1) was found in this region as a putative transcription factor binding site. USF1 is binding with E-boxes which has a consensus sequence of CACGTG. USF1 is a ubiquitously expressed transcription factor and involved in the transcriptional control of many genes including the molecular pathogenesis of cardiovascular disease. Thus, we investigated that the putative functional relationship between the minisatellites variants and susceptibility for myocardial infarction. A case-control study was performed that compared genomic DNA from 400 controls and 225 cases with myocardial infarction. There were no significant differences observed in the overall allelic distribution of minisatellites between controls and cases, which indicates that this polymorphism is not responsible for myocardial infarction susceptibility. Hence, we analyzed the five different minisatellites alleles from this study and characterized 14 different repeats units (Unit1~Unit14). Then, we evaluated the DNA composition, phylogenic tree, and pairwise distances of its repeats. The variability of each repeats differed from 2.33% to 16%. The phylogenic trees for the four SLC6A19-MS7 minisatellites exhibited very different shapes in their braches and distances, and present most common 8 repeats allele was the longest 14 repeats allele. Therefore, this result may help to understand for the evolutional level of the length of minisatellites.