• 환경위생공학
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• 제8권1호
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• pp.81-91
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• 1993

This paper was carried out to isolate and identify Aeromonas caviae which can degrade Sodium Dodecyl Benzene Sulfonate(SDBS) effectively. And the affecting factors for the ability of bacterial degradation were also studied. Frm October 1991 to February 1992, two hundred samples from sweage in Taegu area and Nakdong river waters in Talsung Gun area were tested. Minimal salt medium which contain SDBS only as a carbon source was used as a culture medium. The isolated new strain was identified as Aeromonas caviae Kim & Kweon. The optimal pH for SDBS degradation were 7.0 and temperature, $32^{\circ}C.$ It was taken 24 hours to degrade SDBS of 20mg/l completely under the optimal pH and temperature. And in the case of 30 mg/l of SDBS, it was taken 36 hours. The nitrogen sources were added to the minimal salt media containing 20mg/l of SDBS, and they were incubated at $32^{\circ}C$ for 14 hours. 86.9% SDBS were degraded after addition of 0.03% peptone as a organic nitrogen source. And 70.5% SDBS after addition of 0.05% ammonium sulfate as a inorganic nitrogen source. In the case of metal compounds(0.015%), the degradation rate for SDBS were 3.5 fold increased in the media containing magnesium chloride and calcium chloride than in the media that were not containing these metal compounds. And where the media containing magnesium chloride was 0.05%, the degradation rate was 65.8%. And above 0.3% NaCI, the degradation rate was decreased slowly.

• BMB Reports
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• 제33권5호
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• pp.422-425
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• 2000

Thioltransferase (TTase), also known as glutaredoxin (Grx), is an enzyme catalyzing the reduction of a variety of disulfide compounds and acting as a cofactor for various enzymes such as ribonucleotide reductase. The Schizosaccharomyces pombe cells, exponentially grown in rich medium at $30^{\circ}C$, were shifted to $20^{\circ}C$ and $35^{\circ}C$. The yeast cells, shifted to $35^{\circ}C$, showed higher TTase activity than the cells continuously grown at $30^{\circ}C$, whereas the yeast cells, shifted to $20^{\circ}C$, gave lower TTase activity. The S. pombe cells, exponentially grown in minimal medium and shifted from $30^{\circ}C$ to $35^{\circ}C$ and $40^{\circ}C$, produced higher TTase activity. When the S. pombe cells were initially incubated in rich and minimal media at three different temperatures ($25^{\circ}C$, $30^{\circ}C$ and $35^{\circ}C$), they showed higher TTase activity at higher temperature. These results suggest that the TTase activity of S. pombe is regulated by temperature.

• 공업화학
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• 제20권4호
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• pp.449-452
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• 2009

철,셀레늄,그리고 몰리브데늄이 결여되어 있는 최소배지는 Escherichia coli MC4100의 혐기성 배양에서의 수소생산을 억제하였다. 철, 셀레늄, 그리고 몰리브데늄은 대장균과 Enterobacter 모두의 formate dehydrogenase ($FDH_{II}$) 효소의 보조인자로 알려져 있다.그러므로 이러한 미량성분들이 최소배지에 결여되어 있을 때는 $FDH_{II}$를 통한 수소생산이 대장균 뿐만 아니라 E. aerogenes에서도 저해될 것이다. 이러한 성분들이 부족할 때 E. aerogenes 413에 의한 수소 생산은 지연되었다.그러므로,E. aerogenes에 의한 수소 생산은 NADH의 재산화가 아닌 포메이트 탈카복시 반응에 의해서 시작된다고 사료된다.

• Journal of Plant Biotechnology
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• 제48권2호
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• pp.93-99
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• 2021

This study was conducted to develop an Agrobacterium-mediated genetic transformation protocol for the carnation cv. "Jinju" to counteract its ethylene sensitivity. The new protocol involves the use of an improved shoot regeneration medium, optimized minimal concentrations of the selective agent, a pre-culture period, and co-cultivation periods. Silver nanoparticles (NAg) added at a concentration of 2.0 μM to the Murashige and Skoog (MS) basal shoot regeneration medium supplemented with 0.1 mg/L indole-3-butyric-acid (IBA) and 0.2 mg/L thidiazuron (TDZ) improved the shoot regeneration efficiency, number of shoots per explant, and plant growth compared to the control without the addition of NAg. The phosphinothricin (PPT) concentration of 1.0 mg/L was determined to be the minimal and optimal concentration for the selection of putative transgenic plants. When the explants were infected with Agrobacterium cells harboring the acdS gene, the explants that were pre-cultured for three days induced more putative transgenic plants than those that were co-cultivated for four days. Therefore, we expect that the results of this study will benefit researchers who are developing genetic transformations of carnations.

• 한국미생물·생명공학회지
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• 제38권2호
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• pp.207-215
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• 2010

미생물 군집의 차이를 이용한 한국산 및 중국산 김치의 판별 가능성을 평가하기 위해 pH 5 이상의 발효초기 김치에 존재하는 bacteria의 다양성을 검토하였다. marine medium, nutrient medium, succinate minimal medium(SMM), leuconostocs selective medium(LUSM)의 한천배지를 이용하여 한국산 26종, 중국산 22종의 김치시료로부터 각각 45 속의 1017 균주, 54 속의 842 균주가 분리동정되었다. 한국산 김치에서는 Bacillus, Weissella, Leuconostoc, Pseudomonas, Lactobacillus 속 순으로, 중국산 김치에서는 Bacillus, Weissella, Lactobacillus, Pseudomonas, Serratia, Enterobacter 속의 순으로 우점하는 것으로 나타났다. 유산균의 경우, LUSM의 이용으로 한국산, 중국산 모두에서 Weissella 속이 편중되어 검출되었지만, 한국산은 Leuconostoc속이, 중국산은 Lactobacillus 속이 Weissella 속 다음으로 우점하는 것으로 나타났다. Weissella confusa는 한국산 김치에서 특이적으로 검출되었고, W. soli, Serratia proteamculans는 중국산 김치에서 특이적으로 검출되어 향후 김치의 원산지 판별을 위한 지표미생물로 사용될 가능성을 가지고 있다.

• Clinics in Shoulder and Elbow
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• 제18권4호
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• pp.197-205
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• 2015

Background: The purpose of this study was to evaluate the postoperative magnetic resonance imaging (MRI) results of minimal-tying (one medial-row tie among 4 medial-row sutures) on the medial-row in double-row suture-bridge configuration ($2{\times}2$ anchor with $4{\times}4$ suture stands). Methods: From 2011 March to 2012 July, 79 patients underwent arthroscopic rotator cuff repair using $2{\times}2$ anchor double-row configuration. The mean age was 61.3 years (range, 31-81 years). Two double-loaded suture anchors were used for medial-row. Four medial-row stitches were made with only one medial-row knot-tying (the most anterior suture). Lateral-row was secured using the conventional suture-bridge anchor technique; all 4 strands were used for each anchor. Repair integrity was evaluated with MRI at mean 6.2 months postoperatively. Retear and the pattern of retear, change of fatty infiltration, and muscle atrophy of supraspinatus were evaluated using pre- and postoperative MRI. Results: Repaired tendon integrity was 38 for type I, 30 for type II, 6 for type III, 4 for type IV, and 1 for type V, according to Sugaya classification. Considering type IV/V as retear, the rate was 6.3% (5 out of 79 patients). Medial cuff failure was observed in 4 patients. Fatty atrophy of supraspinatus was significantly improved postoperatively according to Goutallier grading (p=0.01). The level of muscle atrophy of supraspinatus was not changed significantly after surgery. Conclusions: Minimal tying technique with suture configuration of four-by-four strand double-row suture-bridge yielded a lower retear rate (6.3%) in medium to large rotator cuff tears.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1236-1241
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• 2007

Five fusion proteins between Z domains derived from Staphylococcal Protein A and Green Fluorescent Protein or Human Proinsulin were produced on the periplasm of Escherichia coli. The effects of the molecular weight and amino acid composition of the translocated peptide, culture medium composition, and growth phase of the bacterial culture were analyzed regarding the expression and periplasmic secretion of the recombinant proteins. It was found that secretion was not affected by the size of the translocated peptide (17-42 kDa) and that the highest periplasmic production values were obtained on the exponential phase of growth. Moreover, the highest periplasmic values were obtained in minimal medium, showing the relevance of the culture medium composition on secretion. In silico prediction analysis suggested that with respect to the five proteins used in this study, those that are prone to form ${\alpha}$-helix structures are more translocated to the periplasm.

• Biotechnology and Bioprocess Engineering:BBE
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• 제2권2호
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• pp.69-72
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• 1997

The pH of microbial culture medium was calculated from equations of equilibrium, meterial balances for ionic components and electro-neutrality theory. Ammonium ion consumption and Acetic acid production are found out to be the major contributors for the alteration of the pH as well as the buffer capacity of the medium. By measuring the buffer capacity on-line, levels of acetic acid were estimated by a software sensor using pH signal in a fermentation process of E.coli growing in a minimal medium. The measured values of acetic acid showed good correlation to those of estimated by the software sensor.

• KSBB Journal
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• 제13권2호
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• pp.119-124
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• 1998

Twelve bacterial strains capable of growing on phenol minimal medium were isolated from iron foundry activated sludge by enrichment culture, and amount them, one isolate which was the best in cell growth and phenol degradation was selected and identified as Acinetobacter junii POH. The optimal temperature, initial pH and phenol concentration in the above medium were 3$0^{\circ}C$, 7.5 and 1000 ppm, respectively. Cell growth of Acinetobacter junii POH dramatically increased 20 hrs cultivation-time and reached a almost stationary phsae 40 hrs cultivation-time then phenol was degraded about 98%. Cell growth was inhibited y phenol at concentrations over 1500 ppm. The isolate was resistant to several antibiotics as well as various heavy metal ions. The growth-limiting log P value of Acinetobacter junii POH on organic solvents was 2.9 in the LB medium. Therefore, it is suggested that Acinetobacter junii POH could be effectively used for the biological treatment of wastewater containing the presence of heavy metal ions and organic solvents.

• 한국균학회지
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• 제41권4호
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• pp.231-235
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• 2013

붉은자루동충하초의 이용성을 증대하기 위한 기초자료로서 확보하고자 배지종류, 배양법, 산도, 탄소원, 그리고 질소원이 균사생장에 미치는 영향을 4개 균주를 대상으로 재평가하였다. 시험한 8종의 고체배지에서의 균사생장은 배지 종류 및 배양일수에 따라 균주간에 차이가 있었다. 8종의 고체배지 중 PDA 및 MMMA(mushroom minimal medium agar)서 균사생장이 우수하였다. 반면에 8종의 액체배지 중에서는 4개 균주 모두 SDYM(Sabourand's dextrose yeast extract medium) 배지에서 모두 우수한 균사생장을 보였다. 액체배지에서의 균사생장은 pH 4.0에서 pH 9.5 사이의 범위에서 큰 차이 없이 양호한 생장을 보였다. 일부 균주는 pH 8.0에서 pH 9.5의 알카리 조건에서 생장이 양호하였다. 탄소원의 경우 xylose 이용 능력이 균주간에 차이가 크게 나타났다. 질소원 경우에 Urea 이용 능력이 있는 균주가 존재함이 새로이 확인되었다. 이런 결과는 기존에 보고되지 않은 붉은자루동충하초의 새로운 균사생육 특성을 보여준다. 결론으로서 향후 새로운 붉은자루동충하초 균주를 이용하고자 할 경우는 반듯이 사전에 적정 균사생육 조건을 검토하는 것이 필요하다고 사료된다.