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Analysis of Phytochemicals in Popular Medicinal Herbs by HPLC and GC-MS (HPLC와 GC/MS를 활용한 약용식물 유래 phytochemicals 분석)

  • Cho, Hyun-Jung;Yoo, Dong-Chan;Cho, Hyun-Nam;Fan, Lu-An;Kim, Hee-Joon;Khang, Kong-Won;Jeong, Ho-Soon;Yang, Seun-Ah;Lee, In-Seon;Jhee, Kwang-Hwan
    • Korean Journal of Food Science and Technology
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    • v.40 no.3
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    • pp.277-282
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    • 2008
  • Oriental herbs are reported as having potent functions for preventing many types of diseases. They also appear to have positive effects and potential capabilities for skin care. Among the many oriental herbs that are available, we chose to analyze four medicinal herbs, Korean red ginseng, Artemisia capillaries Thunb, Schizonepeta tenuifolia Briq, and Foeniculum vulgare Mill, because all are popular and considered as favorite medicinal plants in Korea. Extracts of the herbs were obtained by various methods such as using distilled water, ethyl ether, methanol, ethanol, benzene, 1-butanol, and chloroform. Nine phytochemicals were detected in the extracts: maltol, adenosine, b-pinene, menthone, pulegone, limonene, anethole, estragole, and fenchone, which reportedly have multi-functionalities. All phytochemicals were analyzed quantitatively by various chromatographic techniques such as HPLC and gas chromatography-mass (GC-MS) spectrometry. This article also presents the optimum conditions for extracting these 9 targeted phytochemical compounds that were derived from 4 popular oriental herbs, which could be useful for the efficient preparation of each phytochemical.

A Study on the Development of High Permeability and Low Coercivity Ni-Zn Ferrite (고투자율, 저보자력을 갖는 Ni-Zn Ferrite의 개발에 관한 연구)

  • 고재귀
    • Journal of the Korean Magnetics Society
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    • v.7 no.1
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    • pp.13-18
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    • 1997
  • The effects of the various raw material composition and sintered temperature on the physical properties of Ni-Zn ferrite have been investigated. They turned out to be spinel structure by X-ray diffraction and the size of grain from microscope was from 6 ${\mu}{\textrm}{m}$ to 16 ${\mu}{\textrm}{m}$. As the sintering temperature was increased from 1030 $^{\circ}C$ to 1070 $^{\circ}C$, the initial permeability and magnetic induction has increased and the both of Q factor and coercive force has decreased. The coercive force and curie temperature were almost the same at each specimen. Their values were about 0.20 Oe and 220 $^{\circ}C$. The frequency of specimen will used in the range from 400 kHz to 20 MHz. The basic composition of $Ni_{0.14}Zn_{0.64}Cu_{0.22}Fe_2O_4$ (specimen B) sintered at 1050 $^{\circ}C$ shows the best results at magnetic induction($B_r & B_m$).

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Effects of Dietary Pearlzyme on Growth Performance and Development of Digestive Organs in Broilers (펄자임 첨가사료가 육계의 생산성과 소화기관의 발달에 미치는 영향)

  • Kim, Jimin;Kang, Seokmin;Yoon, Jeong Yong;Yang, Young-Rok;Kim, Won;Jang, Jung-Soon;Choi, Yang-Ho
    • Korean Journal of Poultry Science
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    • v.42 no.4
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    • pp.291-297
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    • 2015
  • We investigated the effects of dietary pearlzyme (mudflat-bacteria origin protease) on growth performance and development of digestive organs in broilers. Two hundred forty, 4 day-old female Ross broiler chicks were divided into 2 groups (control vs. pearlzyme) which were randomly housed in 8 pens with 15 chicks/pen. They were fed one of two diets containing pearlzyme at 0 or 0.1% for 4 weeks. Dietary pearlzyme resulted in significant increase in body weight during the first week of the experiment (p<0.05). With age, weight gain and feed efficiency continued to decrease reaching significant level during the last week. Mortality was 3.3% in control but not in pearlzyme during the entire period of the experiment. Dietary pearlzyme resulted in increased weight (p<0.05) in the ceca and rectum, and increased length in the ceca (p<0.05). However, there were tendencies to increase the weight of the gizzard (p<0.071) but to decrease the length of the small intestine (p<0.068). The results of the current study show that dietary pearlzyme affects weight gain and the development of digestive organs.

Physicochemical Properties of Endosperm Starch and Breadmaking Quality of Rice Cultivars (쌀 품종의 배유 이화학적 특성에 따른 제빵 적성 비교)

  • Yoon, Mi-Ra;Chun, A-Reum;Oh, Sea-Kwan;Ko, Sang-Hoon;Kim, Dae-Jung;Hong, Ha-Cheol;Choi, Im-Soo;Lee, Jeong-Heui
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.3
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    • pp.219-225
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    • 2011
  • The physicochemical properties of rice endosperm from five rice varieties and effects of milling on baking bread properties of rice flour were investigated. Five rice varieties exhibited different level of amylose content. The ratio of longer amylopectin chain length to the distribution of endosperm starches was the highest in Goami3. According to the RVA measurement of rice flours, the pasting temperatures of Seolgaengbyeo and Goamibyeo were lower than those of the other rice varieties. There were differences in the changes of swelling power of rice flour under increasing temperature. Each rice flour sample for bread-making was processed into two different particle size by using an air-classification mill, and significant differences were observed among cultivars in the average particle size and damaged starch content of rice flours. Rice flour of Seolgaengbyeo with fine particle size showed the highest value in specific volume after baking.

Effect of Elevated Carbon Dioxide Concentration and Temperature on Yield and Fruit Characteristics of Tomato (Lycopersicon esculentum Mill.) (이산화탄소 및 온도 상승이 토마토 수량 및 과실특성에 미치는 영향)

  • Lee, In-Bog;Kang, Seok-Beom;Park, Jin-Myeon
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.428-434
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    • 2008
  • The objective of this study is to investigate the effect of the level of $CO_2$ (370 and $650{\mu}mol\;mol^{-1}$) and temperature (ambient and ambient+$5^{\circ}C$) on tomato growth and fruit characteristics as affected by the application rate of N-fertilizer (68 and $204\;N\;kg\;ha^{-1}$), for the purpose of evaluating the influence of elevated $CO_2$ and temperature on tomato crop. The elevated atmospheric $CO_2$ and temperature increased the plant height and stem diameter for tomato crop, while the differences among the nitrogen(N) application rates were not significantly different. Under the elevated $CO_2$, temperature, and a higher N application rate, the biomass of aerial part increased. The fruit yield showed the same result as the biomass except for the elevated temperature. The elevated temperature made the size of fruit move toward the small, but the elevated $CO_2$ and the application of N-fertilizer were vice versa. The sugar content and pH of fruit juice were affected by nitrogen application rate, but not by the elevated $CO_2$ and temperature. These results showed that both the elevated $CO_2$ and temperature stimulated the vegetative growth of aerial parts for tomato, but each effects on the yield of fruit showed an opposite result between the elevated temperature and $CO_2$. In conclusion, the elevated $CO_2$ increased tomato yield and the ratio of large size of fruit, but the elevated temperature did not. Therefore, to secure the productivity of tomato as nowadays in future environment, it will need to develop new breeder as high temperature-tolerable tomato species or new type of cropping systems.

Changes of Fruit Cracking Percentage and Fruit Shape of 'Hei' Black Tomato with Increased Temperature (온도증가에 따른 흑색토마토 '헤이' 품종 과실의 모양 및 열과 발생률의 변화)

  • Moon, Doo-Gyung;Kim, So-Hee;Cho, Myeng-Whan;Yu, In-Ho;Ryu, Hee-Ryong;Lee, Eung-Ho
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.17 no.3
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    • pp.202-206
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    • 2015
  • 'Hei' (Lycopersicon Esculentum Mill.) Black Tomato examined changes in fruit shape, fruit weight and cracking percentage with increasing temperature in high-rise tomato greenhouse during long-term cultivation in summer season. Fruit diameter is greater than fruit length from the first cluster to the ninth cluster. However, fruit length is longer than fruit diameter from the tenth cluster to the fifteenth cluster. Fruit shape index (L/D) is below 100% from the first cluster to the ninth cluster and above 100% from the tenth cluster to the fifteenth cluster. Fruit weight was decreased during temperature increasing in greenhouse. However, fruit cracking percentage was increased to temperature increasing during cultivation period. Thus, fruit shape, fruit weight and fruit cracking of 'Hei' black tomato were influenced by increasing of temperature in greenhouse during long-term cultivation.

Growth Characteristics of Herbaceous Plants by Soil Condition to Revitalize the Urban Agriculture (도시농업 활성화를 위한 토양조건별 초본식물의 생육특성)

  • Park, Won Jei;Han, Kyung Hwan;Kwon, Soon Hyo;Park, Mi Ok;Koo, Bon Hak
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.15 no.2
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    • pp.1-8
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    • 2012
  • This study is carried out to find the most optimal soil-plant combination in the urban agriculture by analyzing the association of soil base material which is being used in the urban agriculture with the growth of plants. 4 types of easily purchased soil (bed soil(A), animal vermicast soil(B), earth worm soil(C) and matured compost(D)) verified in aspects of effect and safety of soil in terms of growth of crop is selected as experimental soil and B, C, D type soils are mixed with granite soil at the ratio of 7 : 3. And granite soil(E) is set as a controlled soil and is compared to verify the effect of the experimental mixed soil. Herbaceous plants are classified into the fruit vegetables (Lycopersicon esculentum Mill. and Capsicum annuum L.), leafy vegetables (Brassica campestris L. ssp. Pekinensis and Lactuca sativa L.), medicinal vegetables (Chrysanthemum zawadskii var. latilobum and Liriope platyphylla F. T. Wang & T. Tang). The results of comparison of growth of herbaceous plants in different soil types showed that fruit vegetables and leafy vegetables in general had excellent growth in D type soil mixture and A type soil in general and had the poorest growth in E type (controlled) soil. 'Chrysanthemum zawadskii var. latilobum' had the excellent growth in D type, B type, C type mixed soil and A type soil and E type (controlled) soil are followed in order. In the case of 'Liriope platyphylla F. T. Wang & T. Tang', the difference in growth by each soil was shown to be insignificant. Therefore, the soil applied in the urban agriculture varies depending on each species of herbaceous plants, but it is considered effective to cultivate herbaceous plant which is economical and productive by using D type mixed soil which can be recycled and inexpensive compared with other experimental soils in the urban agriculture.

Structural Properties of Cold Water Extractable ${\alpha}-D-glucan$ in Rice Flours (쌀가루 냉수추출 ${\alpha}-D-glucan$의 분자구조적 특성)

  • Park, Yong-Kon;Seog, Ho-Moon;Nam, Young-Jung;Choi, Kwang-Soo
    • Korean Journal of Food Science and Technology
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    • v.22 no.6
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    • pp.603-610
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    • 1990
  • The structural properties of cold water extractable ${\alpha}-D-glucan$ in rice flours obtained by various milling methods were investigated. The blue value, ${\lambda}_{max}$ values of the iodine complex and ${\beta}-amylolysis$ limit of the cold water extractable ${\alpha}-D-glucan$ were in the range of $0.026{\sim}0.030,\;518{\sim}522\;nm$ and $52.7{\sim}59.6%$, respectively, indicating these materials were composed mainly of amylopectin-like polymer. The gel chromatography on Sepharose CL-2B indicated that the cold water extractable ${\alpha}-D-glucan$ had lower molecular weight but wider molecular weight distribution than that of the original rice amylopectin. The Sephadex G-50 gel chromatography showed that the unit chain distribution of cold water extratable ${\alpha}-D-glucan$ after debranching with pullulanase was similar to that of rice amylopectin. And the ion chromatography of the distribution pattern of the chain length below D.P.30 in the debranched ${\alpha}-D-glucan$ indicated only blade-milled flour was similar to the amylopectin, but in the amount of unit chain below D.P.10, all the ${\alpha}-D-glucan$ in rice flours was higher than that of amylopectin.

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Effect of Cytokinin and Auxin on Tomato Leaf Segment Culture (토마토의 조직배양(組織培養)에 있어 Cytokinin과 Auxin의 영향(影響))

  • Lee, Young Bok;Kim, Myeong Won;Cho, Seong Sup
    • Korean Journal of Agricultural Science
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    • v.13 no.2
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    • pp.168-175
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    • 1986
  • Leaf segments of tomato (Lycopersicum escullentum Mill) were cultured on the MS medium supplemented with the concentration of 0, 0.2, 0.5, 2.0 or $5.0mg/{\ell}$ NAA, 2,4-D and/or BA. The treatments were able to induced callus, however the best combinations for the induction of callus were $2.0mg/{\ell}$ BA and 2.0 or $0.5mg/{\ell}$ NAA. Shoot formation was stimulated at the treatment of 2.0 or $5.0mg/{\ell}$ BA, and root formation was stimulated on the medium of 0.2, 0.5, 2.0 or $5.0mg/{\ell}$ NAA plus 0, 0.2 or $0.5mg/{\ell}$ BA.

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On Preparation and Effects of Composts from Industrial Wastes via High Temperature and Aerobic conditions (유기성 산업폐기물의 고온·호기성 퇴비화 및 비효평가)

  • Kweon, Jin-Wook;Lee, Kyu-Seung;Park, Seung-Heui
    • Korean Journal of Agricultural Science
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    • v.22 no.2
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    • pp.143-150
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    • 1995
  • In order to find a way to utilize paper mill sludge, its composting was conducted with anaerobic waste of kraft paper sludge, raw kraft paper sludge, and CMC sludge(CMC : Sodium Carboxymethylcellulose) under aerobic condition at $50^{\circ}C$. It took 3 days for initial fermentation with anaerobic waste and CMC sludge, and six days with raw kraft paper sludge. Each compost was applied to radish(Rhaphanus Stativus L.), and absorption rate of staple nutrients increased 6.7~9.3 times higher in N, 17~21 times in P and 2~3 times in K than control at the harvesting stage. Also, organic matter contents were increased 1.5~2.3 times 4.5~5.3 times in CEC compared to control soil.

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