• Journal of Korean Neurosurgical Society
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• 제42권2호
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• pp.118-124
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• 2007

Objective : Adipose tissue is derived from the embryonic mesoderm and contains a heterogenous stromal cell population. Authors have tried to verify the characteristics of stem cell of adipose derived stromal cells (ADSCs) and to investigate immunohistochemical findings after transplantation of ADSC into rat brain to evaluate survival, migration and differentiation of transplanted stromal cells. Methods : First ADSCs were isolated from human adipose tissue and induced adipose, osseous and neuronal differentiation under appropriate culture condition in vitro and examined phenotypes profile of human ADSCs in undifferentiated states using flow cytometry and immunohistochemical study. Human ADSCs were transplanted into the healthy rat brain to investigate survival, migration and differentiation after 4 weeks. Results : From human adipose tissue, adipose stem cells were harvested and subcultured for several times. The cultured ADSCs were differentiated into adipocytes, osteoctye and neuron-like cell under conditioned media. Flow cytometric analysis of undifferentiated ADSCs revealed that ADSCs were positive for CD29, CD44 and negative for CD34, CD45, CD117 and HLA-DR. Transplanted human ADSCs were found mainly in cortex adjacent to injection site and migrated from injection site at a distance of at least 1 mm along the cortex and corpus callosum. A few transplanted cells have differentiated into neuron and astrocyte. Conclusion : ADSCs were differentiated into multilineage cell lines through transdifferentiation. ADSCs were survived and migrated in xenograft without immunosuppression. Based on this data, ADSCs may be potential source of stem cells for many human disease including neurologic disorder.

• 한국농림기상학회지
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• 제25권1호
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• pp.37-47
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• 2023

The black cutworm, Agrotis ipsilon (Hufnagel), is an important crop pest worldwide that feeds more than 80 plant species including cabbage, potato, maize, wheat and bean, and this moth is a typical pest attacking underground parts of crops. It has been known in farm booklets that the larvae of A. ipsilon overwinter in the soil in Korea, but no definitive data exist yet. This study was conducted to evaluate that the specific appearance time of A. ipsilon observed actually in the field could be explained when we assumed that this pest overwinters in a form of larvae or pupae. Degree day-based phenology models were applied for tracking forward or backward to find the predicted developmental stage which developed at a specific stage found in the field. As a result of the analysis, it was confirmed that an initial population could be established in a group that does not overwinter as larvae or pupae in Korea. In other words, the appearance of adults in early March to April could not be explained by the presence of domestic overwintering populations. Populations that overwinter as larvae or pupae in Korea were able to emerge as adults in June to July at the earliest. Therefore, the group of adults appearing in early spring is highly likely to be a population that migrated from outside Korea. Taken together, it was estimated that the colony of A. ipsilon in Korea would be formed by a mixture of a migrant population through long-distance migration and a overwintering population.

• 한국수산과학회지
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• 제30권1호
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• pp.39-45
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• 1997

쿠마류 Bodotria biplicata의 조하대 대상분포를 돌산도 모래해안의 쇄파대에서 조사했다. 1993년 1월 소조와 대조때 수심 1m의 표층과 저층, 그리고 바닷가에서 쿠마류를 1조석주기 동안 한 시간마다 썰매 네트로 3회 반복 채집했다(시료수=225개). 조하대에서 가장 우점한 B. biplicata는 저층에서 밀도가 가장 높았고 표층에서는 전체 생물량의 약 $0.6\%$가 채집되었다. 소조때 평균 밀도는 대조때보다 조금 높았다. 채집 생물량은 조하대 수심의 영향을 받았다. 밀도 변화는 주야주기나 밀물-썰물보다 조하대 수심과 더 관련이 있었다. 조사기간에 주야 수직 이동은 뚜렷하지 않았다. 소조와 대조때 모두 B. biplicata 개체군은 낮은 저조 (LLW) 아래 약 90cm 되는 곳에서 밀도가 가장 높았다. 그러므로 이 종은 2주 주기로 해안쪽과 바다쪽으로 수평이동하는 듯하다. B. biplicata가 조하대 모래바닥을 이동하는 속도와 거리는 모래해안의 기울기와 조차에 따라 달라질 것이다. 개체발생 단계별로 분포양상이 달랐고 미성체와 만카유생이 성체보다 깊은 곳에 분포했다. 이것은 먹이를 둘러싼 종내경쟁을 줄이는데 기여할 것 같다.

• Journal of Periodontal and Implant Science
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• 제28권1호
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• pp.145-160
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• 1998

The ultimate goal of periodontal therapy is the regeneration of periodontal tissue which has been lost due to destructive periodontal disease, and numerous kinds of materials and techniques have been developed to achieve this goal. Bone grafts include autografts, allografts, xenografts and synthetic grafts. Among the synthetic grafts, bioactive glass has been used in dentistry for more than ten years and Fetner reported improved new bone formation and more amount of new attachment after grafting PerioGlas, a kind of bioactive glass, in 2-wall defects of monkeys in 1994. It Is well known that 1-wall defects have less osteogenic potential and more epithelial migration, so we need to study the erect of bioactive glass in 1-wall dejects in dogs. The present study evaluates the effect of bioactive glass on the epithelial migration, alveolar bone regeneration, cementum formation and gingival connective tissue attachment in intrabony detects of dogs. Four millimeter deep and four millimeter wide 1-wall defects were surgically cheated in the mesial aspects of premolars. The test group received bioactive glass with a flap procedure and the control underwent flap procedure only. Histologic analysis after 8 weeks of healing revealed the following results: 1. The height of gingival margin was 1.30{\pm}0.73mm$ above CEJ in the control and $1.40{\pm}0.78mm$ in the test group. There was no statistically significant difference between the two group. 2. The length of epithelial growth (the distance from CEJ to the apical end of JE) was $1.74{\pm}0.47mm$ in the control and $1.12{\pm}0.36mm$ in the test group. These was a statistically significant difference between the two groups (P<0.01). 3. The length of new cementum was $2.06{\pm}0.73mm$ in the control and $2.62{\pm}0.37mm$ in the test group. There was no statistically significant difference between the two groups. 4. The length of new bone was $1.83{\pm}0.74mm$ in the control and $2.39{\pm}0.59mm$ in the test group. There was no statistically significant difference between the two groups. These results suggest that the use of bioactive glass 1-wall intrabony defects has significant effect on the prevention of junctional epithelium migration, but doesn't have any significant effect on new bone and new cementum formation.

• 생명과학회지
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• 제20권9호
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• pp.1287-1293
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• 2010

RAPD에 의한 지리산 내 산거울 집단의 유전자 빈도와 지리적 거리에 따른 공간적 상관관계를 분석하였다. 전체 102 DNA 분절(밴드)이 107 개체에서 탐지되었다. 102 밴드 중 48(47.1%)개 밴드는 다형성을 나타내었다. 분집단간 다형성의 비교에서 거리 구간 I와 V가 가장 낮은 변이(16.7%)를 나타내었고, 거리 구간 VIII이 가장 높은 변이를 나타내었다(22.6%). 전체 다양도는 0.076이었다. 구간 VIII이 가장 높은 다양도(0.093)를 나타내었고, 구간 I가 가장 낮았다(0.063). 구간 사이의 유전적 유사도는 60 m 거리까지는 유사하였다. 지리산 집단에서 산거울은 강한 공간구조를 나타내고 있음이 RAPD 마커로 알 수 있었다. 이는 지리산 집단에서 낮은 이주자수와 개체들이 덩어리 모양의 분포를 나타내기 때문으로 판단된다. 본 연구에서 RAPD 마커로 산거울의 공간구조와 유전적 구조를 파악하는데 유용하게 이용될 수 있음을 입증하였다.

• 생태와환경
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• 제44권4호
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• pp.385-395
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• 2011

본 연구는 하천준설 및 골재채취로 인하여 2개의 웅덩이가 직렬로 발생하였을 때, 이동상 실내실험을 수행하여 웅덩이의 적응과정과 응답특성을 분석하였다. 웅덩이의 되메우기 과정은 웅덩이 상류에서 발달한 사주의 영향을 받지만, 웅덩이 하류에서는 웅덩이에서 유사가 포착되어 사주가 발달되지 않았으며, 하상이 지속적으로 저하되었다. 웅덩이의 되메우기가 진행될 때, 웅덩이의 이동속도는 거의 일정하며, 웅덩이의 수중 안식각은 일정하게 유지되었다. 그러나 웅덩이의 되메우기가 거의 완료되면서, 웅덩이의 이동속도는 증가하고, 웅덩이에서 수중 안식각은 작아졌다. 웅덩이와 웅덩이의 이격거리가 증가하면, 2차 웅덩이 하류에서 미부침식이 크게 발생하였다. 또한 웅덩이의 이격거리가 증가할수록 웅덩이의 이동속도는 감소하였다. 이것은 웅덩이가 되메워지는 데 시간이 증가하고, 웅덩이 하류하천에 미치는 영향이 크다는 것을 의미한다. 시간이 증가함에 따라, 무차원 웅덩이의 깊이는 지속적으로 감소하지만, 웅덩이의 이격 거리가 증가할수록, 무차원 웅덩이 깊이는 증가하였다. 그러나 무차원 웅덩이의 길이는 시간이 증가함에 따라서 전체적으로 감소하지만, 웅덩이와 웅덩이의 이격 거리가 증가할수록, 무차원 웅덩이의 길이가 증가하는 경향을 보여주었다.

• Hip & pelvis
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• 제30권4호
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• pp.254-259
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• 2018

Purpose: Internal fixation using compression hip screws (CHS) and traction tables placing patients in the supine position is a gold standard option for treating intertrochanteric fractures; however, at our institution, we approach this treatment with patients in a lateral decubitus position. Here, the results of 100 consecutive elderly (i.e., ${\geq}45$ years of age) patients who underwent internal fixation with CHS in lateral decubitus position are analyzed. Materials and Methods: Between March 2009 and May 2011, 100 consecutive elderly patients who underwent internal fixation with CHS for femoral intertrochanteric fracture were retrospectively reviewed. Clinical outcomes (i.e., Koval score, Harris hip score [HHS]) and radiographic outcomes (i.e., bone union time, amount of sliding of lag screw, tip-apex distance [TAD]) were evaluated. Results: Clinical assessments revealed that the average postoperative Koval score decreased from 1.4 to 2.6 (range, 0-5; P<0.05); HHS was 85 (range, 72-90); and mean bone union time was 5.0 (range, 2.0-8.2) months. Radiographic assessments revealed that anteroposterior average TAD was 6.95 (range, 1.27-14.63) mm; lateral average TAD was 7.26 (range, 1.20-18.43) mm; total average TAD was 14.21 (range, 2.47-28.66) mm; average lag screw sliding was 4.63 (range, 0-44.81) mm; and average angulation was varus $0.72^{\circ}$(range, $-7.6^{\circ}-12.7^{\circ}$). There were no cases of screw tip migration or nonunion, however, there were four cases of excessive screw sliding and six cases of varus angulation at more than $5^{\circ}$. Conclusion: CHS fixation in lateral decubitus position provides favorable clinical and radiological outcomes. This technique is advisable for regular CHS fixation of intertrochanteric fractures.

• 생명과학회지
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• 제18권10호
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• pp.1325-1330
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• 2008

키버들(Salix koriyanagi)은 관목으로 한국에 고유종이다. 전분 젤 전기영동을 사용하여 남강 상류 저습지에 분포하는 키버들 집단에서 유전적 다양성과 집단구조를 분석하기 위해 집단을 세분하였다. Moran's I값으로 세분화 된 1.0 m 간격별 구간 내 개체들의 대립유전자좌위에서 유의한 상관을 조사한 결과 약 6 m 간격 내 개체들의 뭉침이 나타났으며, 12 m 이상일 경우 유의한 차이가 나타났다. 따라서 키버들의 경우 집단구조가 12 m 이내에서 형성됨을 알 수 있었다. 대부분의 집단이 제한된 유전자 유동과 유효집단의 감소가 예상되며, 겨울철 가뭄과 여름철 홍수로 심한 병목을 유발하고, 주기적인 홍수와 배수로 인한 개체 수의 교란이 발생한 것으로 판단된다.

• 생명과학회지
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• 제18권11호
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• pp.1465-1470
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• 2008

키버들(Salix koriyanagi)은 관목으로 한국에 고유종이다. 전분 젤 전기영동을 사용하여 남강 상류 저습지에 분포하는 키버들 집단에서 유전적 다양성과 집단구조를 분석하기 위해 집단을 세분하였다. Moran's I값으로 세분화된 1.0 m 간격별 구간 내 개체들의 대립유전자좌위에서 유의한 상관을 조사한 결과 약 6 m 간격 내 개체들의 뭉침이 나타났으며, 12 m 이상일 경우 유의한 차이가 나타났다. 따라서 키버들의 경우 집단구조가 12 m 이내에서 형성됨을 알 수 있었다. 대부분의 집단이 제한된 유전자 유동과 유효집단의 감소가 예상되며, 겨울철 가뭄과 여름철 홍수로 심한 병목을 유발하고, 주기적인 홍수와 배수로 인한 개체수의 교란이 발생한 것으로 판단된다.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 International Symposium on Clean Environment
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• pp.198-203
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• 2003

The effects of salmon carcasses on forest and stream ecosystems were determined by nitrogen stable isotope analysis in natural streams in Hokkaido, Northern Japan, where numerous chum salmon (Oncoryhncus keta) were migrated upstream ITom ocean to spawn in autumn. The leaves and soils surrounding riparian forest and stream dwelling invertebrates were collected before and after migration. The nitrogen stable isotope ratio $({\delta}^{15}N)$ of riparian vegetation (Salix spp.) were different depending on the presence of salmon and distance from the stream. The $({\delta}^{15}N)$ of stream dwelling invertebrates were different between salmon present and absent stream. This difference was tested using the experiment channel by implanting salmon carcasses. The nitrogen stable isotope ratio of epilithic algae and leaf shredding animals were nearly 3 higher in the salmon implanted treatment suggesting that around 20％ of salmon derived nitrogen was uptake either in algae and leaf shredding invertebrates. These results suggest that the salmon carcasses effects not only on stream primary production but also on primary consumers, which decompose leaves fertilized with nitrogen from carcasses.