• Korean Journal of Agricultural Science
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• v.23 no.1
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• pp.150-158
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• 1996

The potato has been used as a part of key staple foods in Korea. Recently, the demand for the potato tends to increase due to its nutritional Quality, and the Quantity of imports has rapidly been increasing. Contamination of seed potato by pathogens especially could cause a severe reduction of total production. In order to solve the problem, Korea Research Insistute of Bioscience and Biotechnology(KBIBB) developed the new commodity called a "potato microtuber". Before its production and distribution, researches on new commodity should be undertaken in many aspects, especially in terms of economic aspects. The main objectives of this study are to measure the economic value of the potato microtubers and to forecast its spreading effects and to examine the Contingent Valuation Method(CVM) could be accepted in the economic analysis for a new agricultural product, here in potato microtubers. Through the regression results, the consumer behavior analysis had performed for potential users of potato microtubers, and through the demand function derived by the consequence of functional relationship, consumer's surplus was estimated.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.110-114
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• 2010

Highest increase of biomass was observed when tissue-cultured potato (Solanum tuberosum L. cv. Chubaek) shoots were cultured in a liquid medium containing 1/3 MS solution in a 18 L bioreactor, as compared to 1/4 and 1/2 MS solution. The medium containing 1/4 MS solution showed higher increase of shoot biomass than one containing 1/2 MS solution. Potato microtubers were formed when the medium was exchanged with the medium for microtuber formation and incubated under dark condition. The microtubers were observed first at some axillary buds one week after incubation under dark condition and then at most of the axillary buds by the end of 3 weeks. The 1.5 MS liquid medium and $20^{\circ}C$ were optimal conditions. By the end of 6 weeks, more 1,000 microtubers were formed in the 18 L bioreactor. Then, greened microtubers were harvested after one week culture under light condition.

• Journal of Biosystems Engineering
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• v.23 no.4
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• pp.373-380
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• 1998

The contribution of this paper is to provide the methods for the production automation of potato microtuber using the vision process in growth monitoring. The first method deals with computation for the growth density in the primary growth process. The second method addresses cognition process to identify the number and the volume of potato microtuber in secondary growth process. The third is to decide whether potato microtubers are infected by a virus or bacteria in growth process. The computation for the growth density in the primary growth process uses the method of Labeling. The second and third methods use template matching based on color patterns. With the developed method using vision process, this experiment is capable of discriminating weekly growth-rate in primary growth process, 85% cognition rate in secondary process and identifying whether there are infections. Therefore, we conclude that our experimental results are capable of growth monitoring for mass production of potato microtubers.

• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.252-256
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• 1998

A Study was conducted to investigate comparison of in vitro tuberization between normal and transgenic potato plantlets harboring adenosine deaminase gene in potato cultivar of Desiree. In time course study of in vitro tuberization, the rate of tuberization in four lines were increased till 6 weeks. but maintained stil after 7 weeks. Microtuber initiation of transgenic lines, 43 and 39 were faster than other lines, but no difference was observed after 5 weeks compared with normal plantlets. In all transgenic lines, the majoirty of microtubers produced were small(less than 100 mg) and medium(100-200mg) size rather than large size(more than 200 mg). Among 4 lines , line 9 produced the highest number of microtubers per each culture vessel. The results of this experiment suggest that there is no significant difference in microtuber production efficiency between normal and transgenic potatoes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.678-686
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• 1997

This experiment was carried out to develop the mass propagation system for producing plug plantlets using stem cuttings of virus-tree microtubers in potato. Cocopeat, vermiculite, perlite and peatmoss were combined and used as plug nursery media to find out the best combination suitable for the growth of seedlings derived from microtubers. Seedling growth was favored in high temperature (above 2$0^{\circ}C$) and a long-day photoperiod(above 16 hours) condition, while stolons and microtubers formed in outdoor condition. Shoot and root multiplication was not affected by NAA 10mg /1 or IAA 10mg /1 treatment. At the early growth stage of plug plantlets, the number of leaves and roots and the length of root increased significantly when nodes from the upper (near to apex) part of shoots rather than from basal part were taken. But after transplanting, these differences among these characters were not observed. At ninety days after transplanting the plug plantlets in spring time, plant was around 70 to 80cm in height, and the number of stolons and tubers were ten and seven, respectively.

• Journal of Forest and Environmental Science
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• v.24 no.2
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• pp.79-88
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• 2008

Twenty-eight of 44 Indian Ceropegia species are endemic and their survival is threatened. As a step towards conservation, we implied in vitro methods for the sustainable propagule production in C. hirsuta, C. lawii, C. maccannii, C. oculata and C. sahyadrica. Effects of explant, growth regulators, sucrose and photoperiod were studied. High frequency microtuber production was achieved with the seedling-apical buds, grown on MS medium containing 4-6 mg $1^{-1}$ BAP, 3-8% (w/v) sucrose, under continuous illumination. Each microtuber, when subcultured proliferated to form a cluster of secondary microtubers. Every primary and secondary microtuber bore at least one shoot-bud and a root primordium. Each tuber (formed with any of the significantly effective treatments) weighed more than 500 mg, enough to plant directly in non-sterilized soils. Microtubers could be produced and proliferated round the year. Proliferation could be solely attributed to in vitro procedures as these plants bear solitary tubers in vivo. Microtubers could be sprouted in vitro to prepare ready to pot plantlets. As, this novel method succeeded for all five species, though they belong to different eco-physiological backgrounds, we recommend its implementation in the conservation programs for a broader range of Ceropegia species, supported by other integrated strategies.

• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.87-91
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• 1997

In vitro grown microtubers of potato (cv Jaju) were used for introduction of herbicide resistance gene using bombardment with DNA-coated particles. The apical shoot-tip area of newly sprouted microtubers were intensively bombarded. After bombardment, microtubers were germinated and transplanted in a greenhouse. Northern blot analysis indicated that bar gene was expressed in two plantlets. After 5 weeks of growing, commercial herbicide Basta was sprayed to screen the resistant plants. All untransformed potato plants died after 7 days while two transgenic plants survived.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11a
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• pp.87-91
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• 2001

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.24-24
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• 2001

• Proceedings of the Plant Resources Society of Korea Conference
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• 1995.01a
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• pp.14-14
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• 1995