• Applied Microscopy
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• v.11 no.1
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• pp.39-50
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• 1981

An electron microscopical observation was carried out to compare the general shape of the mast cells and structures of granules inside the cells in the stomach of 5 species in 3 orders of Mammals. In convenience, the granules in the cytoplasm were abbreviated as follows: 1) Homogeneous granule, GR1 2) Particulate granule, GR2 a. Dark dense particulate granule, GR2-1 b. Less dense particulate granule, GR2-2 3) Reticular granule, GR5 a. Dark dense reticular granule, GR5-1 b. Light dense reticular granule, GR5-2 In Mammalia including goat, dog, cat, and hamster, most of cytoplasmic organelle were Golgi apparatus and mitochondria, and most of the cytoplasmic granules were highly densed GR1and GR2. However GR5-1 and GR5-2 appeared in guinea pig while one side sunken or crescent-like types occured in both dog and guinea pig. All mast cells were oval or spindle with cytoplasmic processes around the cell. There was also found vacuoles and vesicles in these cells. These results demonstrated that there was a morphological difference between species of vertebrate in the mast cells and their cytoplasmic granules. It was also suggested that a variety of structures of granules were closely related with the composition (histamine, heparin, serotonin, hyaluronic acid etc.) and mature of the granules.

• Journal of Pharmacopuncture
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• v.12 no.3
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• pp.5-24
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• 2009

Injinho-tang(IJ) has been used for the treatment of hepatobiliary diseases. This study was performed to observe the effect of IJ extract on the hepatocellular carcinogenesis and hepatic cirrhosis induced by Diethylnitrosamine(DENA) and $CCl_4$ in Rats. Experimental groups were divided into two ; 8th and 12th week group, and subdivided into four; normal group(Nor), hepatocellular cancer and hepatic cirrhosis inducing control group(Con), and IJ extract 260mg/kg/day(IJA) or 520mg/kg/day(IJB) administered groups to Con. The results obtained are as follows: The body weight was decreased in the Con, IJA and IJB compared with the Nor from the 2nd week to the 12th week. The weight of liver and the weight of liver/100g body weight were decreased significantly in Con, IJA and IJB compared with the Nor. The activities of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) were significantly increased in the Con compared with Nor, but decreased in the IJA and IJB compared with Con from the 8th week group. The activities of alkaline phosphatase(ALP), lactacte dehydrogenase (LDH) and alpha fetoprotein(AFP) were increased significantly in the Con compared with Nor, but decreased in the IJA and IJB compared with Con. The activities of superoxide dismutase(SOD) were decreased in the IJA and IJB compared with Con, but the activities of catalase were increased in the IJA and IJB compared with Con. According to the light and electron microscopical observation, IJA and IJB improved the morphological and histopathological changes of the liver injured by DENA and $CCl_4$. The number of hepatic p53 positive cells was decreased in the IJA and IJB compared with Con. These results suggest that administration of IJ extract suppress or retard DENA and $CCl_4$-induced hepatocelluar carcinogenesis and hepatic cirrhosis in rats.

• The Korean Journal of Zoology
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• v.29 no.1
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• pp.13-22
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• 1986

In order to investigate the 'In Vitro 2-Cell Block' phenomenon found in certain mouse strains such as ICR, the present studies have been done. Fertilized eggs (1-cell) and 2-cell embryos recovered from the oviducts of the ICR mouse at the various time intervals after hCG injection to induce ovulation were cultured for 3 or 4 days to examine the capability for further cleavage beyond 2-cell stage. Consequently, it was found that some proportions of the 1-cell or 2-cell embryos recovered at 30 hours post hCG showed their cleaving capability and if the embryos were obtained after 48 hours of hCG injection, they were all at 2-cell stage and most of them developed to the blastocysts in vitro. It was also found that the embryos obtained at 27 hours post hCG showed their stronger capacity of further development in the groups cultured for shorter period than 24 hours in vitro before transferring to the oviduct. Based on the results, it can be inferred that mouse fertilized eggs should be remained inside the oviduct for a certain length of period after fertilization, or they should be cultured for a short period than 12 hours before returning back to the oviduct in order to develop to blastocysts. It was also found that though the embryos under the 2-cell block in culture showed normal feature up to 24 hours under the microscopical observation, they had already lost their capacity for the normal development, and if the culture of the 2-cell embryos was extended to 48 hours, they showed nuclei with heteropyknosis, and the vacuoles were were detected in the cytoplasm of embryonic cell if they were cultured for 72 hours.

• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.337-346
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• 1993

Experiments were performed to find out the effects of simulated acid rain(SAR) of pH 4.0 or pH 2.5 for 12 weeks on stomatal resistance, wettability and anatomical changes in Quercus acutissima and Ginkgo biloba seedlings. As a control, distilled water with a pH 6.5 was also sprayed. Stomatal resistance of Q. acutissima and of G. biloba remarkably increased after exposure to SAR. SAR increased the wettability of Q. acutissima leaves measured with water blue solution and of G. biloba leaves measured by leaf contact angle method. Anatomical changes in the leaves of Q. acutissima affected by SAR were the partial damage of epidermis and parenchymatous cells. Scanning electron microscopical observation showed that the number of trichomes in the leaves of Q. acutissima treated with SAR markedly decreased and the erosion of epicuticular wax was significant. No distinct damage was found in the G. biloba leaves at pH 4.0, while epidermis and vascular tissue were collapsed at pH 2.5. No significant alteration of surface structures in this tree species was observed.

• Applied Microscopy
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• v.32 no.3
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• pp.231-246
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• 2002

This study was carried out to investigate the effect of squalene (SQ) on the mouse hepatotoxicity induced by cadmium. ICR male mouse weighting about 30 gm were injected $CdCl_2$ (5.0 mg/kg) and SQ (180 mg/kg) into intraperitoneal. At the 1, 2, 3, 4, 5, 6, 7 days, livers were treated with superoxide dismutase (SOD) activity and transmission electron microscopical method and then observed with electron microscope. The results obtained were summarized as follows: SOD activity in the liver, Group A was higher than in normal. Group B was lower than in Group A. In the histological observation, nucleus of Group A showed irregular shape. Inner cavity of mitochondria swellen and development of cristae weakened. Swelling of Lamellae of rough endoplasmic reticulum (RER) showed. Nucleus of group B showed normal shape. Typical lamellae of RER were observed. These results described above treatment of SQ decreased the hepatotoxicity of the $CdCl_2$ and SOD activity in the mouse liver, and then it suggests SQ may be effective for the recovery of hepatic cell.

• Applied Microscopy
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• v.38 no.4
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• pp.353-361
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• 2008

We aimed to investigate whether the extracts of Lycii fructus, Lycii folium, and estradiol can attenuate on the level of cholesterol on serum and ultrastructure alteration of hepatocytes isolated from ovariectomized rats. Experimental groups are divided into five: Sham (sham-operated), OVX (ovariectomized animal), LCF (OVX, Lycii fructus), LCL (OVX, Lycii folium), and $E_2$ (OVX, estradiol). The body weights of OVX group showed increase compared with Sham, LCF, and LCL groups. The levels of total cholesterol and low-density lipoprotein (LDL) cholesterol were significantly decreased in the LCF and LCL groups compared with those of OVX group. Moreover, the High-density lipoprotein (HDL) cholesterol level was increased in the LCL group. As the results of electron microscopical observation, lipid droplets in the OVX group showed increase compared with Sham group. But in the LCF group not showed lipid droplets. In conclusion, administration of Lycii fructus or Lycii folium lowers the serum cholesterol content of the ovariectomized rats and also the damage of the hepatocytes and formation of lipid droplets.

• Applied Microscopy
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• v.28 no.4
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• pp.425-434
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• 1998

This study was designed to investigate the microglial reactions to the neurodegenerative changes in the cat retina. All experiments were performed using adult cats of both sex, weighing $2,500g\sim3,500g$. 5,7-DHT $(100{\mu}g)$ dissolved in 0.1% ascorbic acid was injected into the vitreous body. All injections were performed in one-side eye; the other side served as the control, which was injected only with 0.1% ascorbic acid. Cats were sacrificed at 1, 3, 7, 14 and 21 days after intravitreal injection of 5,7-DHT For light microscopy, retinae were fixed with 4% paraformaldehyde and processed using NDPase histochemistry. Same retinae were fixed with 1% para(formaldehyde-2.5% glutaraldehyde and processed for electron microscopy. NDPase-positive microglial cells were mainly distributed in the inner plexiform layer of the retina, and characterized by a small somata with a few slender processes, which were also extended in the ganglion cell layer (GCL) and inner nuclear layer (INL). The intensity of the microglia stained for NDPase was abruptly increased at 7 day as compared with that of the control, and thereafter continuously sustained until 21 day, the last experimental group in this study. Under the electron microscopical observation, microglial cells in the control group exhibited elongate nucleus with perinuclear chromatin condensation, and the perikaryon was scanty. However, a few hypertrophic glial cells were frequently found at 3 days after the drug injection. By 7 day, most microglial cells directed toward the degenerated neurons in the GCL, and the number of microglial cells was slightly increased as compared with the former group. At the 14 day, most microglial cells wrapped the degenerated cells in the GCL, and a few cells showed phagocytotic features. By 21 day, most microglial cells were engaged in phagocytotic activity, and their cytoplasm was filled with the phagorytosed material. Based on the results, 5,7-DHT may act as a specific neurotoxin to the cat retina, and microglial reactions to the neuronal death are already induced in early experimental stage. These results indicate that the microglial cells in the cat retina show characteristic features as a protective effect of neural tissue.