• Journal of the Korean Society of Radiology
• /
• v.13 no.4
• /
• pp.623-628
• /
• 2019

The aim of this study was to design fast non local means (FNLM) noise reduction algorithm and to confirm its application feasibility in light microscopic image. For that aim, we acquired mouse first molar image and compared between previous widely used noise reduction algorithm and our proposed FNLM algorithm in acquired light microscopic image. Contrast to noise ratio, coefficient of variation, and no reference-based evaluation parameter such as natural image quality evaluator (NIQE) and blind/referenceless image spatial quality evaluator (BRISQUE) were used in this study. According to the result, our proposed FNLM noise reduction algorithm can achieve excellent result in all evaluation parameters. In particular, it was confirmed that the NIQE and BRISQUE evaluation parameters for analyzing the overall morphologcal image of the tooth were 1.14 and 1.12 times better than the original image, respectively. In conclusion, we demonstrated the usefulness and feasibility of FNLM noise reduction algorithm in light microscopic image of small animal tooth.

• Applied Microscopy
• /
• v.37 no.3
• /
• pp.199-208
• /
• 2007

We have made an optical device to study the wave optics phenomena, such as image and diffraction pattern, constructive and destructive interference, by direct operation of laser beam and optical lenses. It consists of laser beam, goniometer, objective lens, intermediate lens, projection lens, CCD system, and computing system. As a result of the performance test, we were able to magnify samples up to 44 times with the resolution of about $5{\mu}m$. It is expected to help EM users understanding more easily principles of transmission electron microscopy (TEM).

• Proceedings of the KIEE Conference
• /
• 2008.04a
• /
• pp.111-112
• /
• 2008

In this study, an image processing algorithm with Lab VIEW IMAQ Module is presented and discussed for the development of a microscopic gap measuring system using CCD sensor. LabVIEW IMAQ development application that is developed by National Instrument Co. Inc. has a graphical interface and many image processing functions, so that it provides an easier environment for the development of a measurement algorithm using the image data. Actual microscopic gap measuring experiment was executed using the algorithm developed in this study and it showed a promising result. Also, we could obtain the convenience of Lab VIEW IMAQ in developing the algorithm.

• Corrosion Science and Technology
• /
• v.15 no.6
• /
• pp.265-270
• /
• 2016

The ellipso-microscopic observation of a titanium surface undergoing anodization in $0.05mol\;dm^{-3}$ of $H_2SO_4$ was conducted. During irradiation by ultra-violet (UV) light with a wavelength of 325 nm, the titanium surface allowed for the flow of a photo-induced current and showed up as a bright, patch-like image on an ellipso-microscopic view. The brightness and patch-pattern in the image changed with flowing photo-induced current. The changes in the brightness and the image corresponded to the formation and/or degradation of titanium oxide due to the photo-electrochemical reaction of the oxide. An in situ monitoring using the ellipso-microscope revealed that the film change was dependent on the irradiation light power, by UV-light increases the anodic current and results in the initiation of pitting at lower potentials as compared with the non-irradiated condition.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.34 no.11B
• /
• pp.1311-1319
• /
• 2009

Microscopic specimen was captured by an integral imaging microscope and displayed as a three-dimensional image by an integral imaging display system. We applied the generalized relationship between pickup and display using two different lens arrays to our integral imaging microscope and display system. In order to display three-dimensional microscopic image, scaling of the captured elemental images is required. We analyzed the effect of the scaling coefficient in terms of the distortion of the displayed three-dimensional image and the loss of the captured elemental images. In our experiment, microscopic specimen is picked up by an integral imaging microscope having $125{\mu}m$ elemental lens pitch and displayed as three-dimensional image by an integral imaging display system having 1mm elemental lens pitch. The scaling coefficient was chosen to minimize the elemental image loss.

• Journal of Multimedia Information System
• /
• v.1 no.1
• /
• pp.87-93
• /
• 2014

The aim of microscopic image restoration is to recover the image by applying the inverse process of degradation, and the results facilitate automated and improved analysis of the image. In this work, we consider the problem of image restoration as a minimization problem of convex cost function, which consists of a least-squares fitting term and regularization terms with non-negative constraints. The finite step method is proposed to solve this constrained convex optimization problem. We demonstrate the convergence of this method. Efficiency and restoration capability of the proposed method were tested and illustrated through numerical experiments.

• Journal of Biomedical Engineering Research
• /
• v.44 no.3
• /
• pp.176-181
• /
• 2023

During cancer treatment, the patient's response to drugs appears differently at the cellular level. In this paper, an image-based cell phenotypic feature quantification and key feature selection method are presented to predict the response of patient-derived cancer cells to a specific drug. In order to analyze the viability characteristics of cancer cells, high-definition microscope images in which cell nuclei are fluorescently stained are used, and individual-level cell analysis is performed. To this end, first, image stitching is performed for analysis of the same environment in units of the well plates, and uneven brightness due to the effects of illumination is adjusted based on the histogram. In order to automatically segment only the cell nucleus region, which is the region of interest, from the improved image, a superpixel-based segmentation technique is applied using the fluorescence expression level and morphological information. After extracting 242 types of features from the image through the segmented cell region information, only the features related to cell viability are selected through the ReliefF algorithm. The proposed method can be applied to cell image-based phenotypic screening to determine a patient's response to a drug.

• ALGAE
• /
• v.19 no.2
• /
• pp.79-90
• /
• 2004

Nuclear DNA contents of spermatia from eight ceramiacean and four dasyacean algae (Ceramiales, Rhodophyta) and microspores from two land plants were estimated by fluorescence microscopic image processing and their nuclear SSU rDNA sequence data were analyzed. In frequency distribution patterns, the DAPI-stained nuclear volume (NV) of spermatia showed two peaks corresponding to 1C and 2C. Nuclear 2C DNA contents estimated from NV were 0.45-2.31 pg in ceramiacean and 0.40-0.57 pg in dasyacean algae and 8.42-9.51 pg in two land plants, Capsicum annuum and Nicotiana tabacum. By nuclear patterning of vegetative cells derived from an apical cell, 2C DNA contents of spermatia were 2.31 pg in an alga having uninucleate and non-polyploid nucleus (Aglaothamnion callophyllidicola), 0.45-1.94 pg in algae having uninucleate and polyploid nucleus (Antithamnion spp. and Pterothamnion yezoense), and 0.40-0.62 pg in algae having multinucleate and non-polyploid nuclei (Griffithsia japonica and dasyacean algae). Each mature spermatium and microspore (pollen grain) seemed to have a 2C nucleus, which may provide a genetic buffering system to protect the genetic content of a spermatium and microspore from potentially lethal mutations. Nuclear DNA content and SSU rDNA sequence of Antithamnion sparsum from Korea were reasonably different from those of Antithamnion densum from France. The data did not support the previous taxonomic studies that these two taxa could be conspecific.

• Applied Microscopy
• /
• v.44 no.4
• /
• pp.138-143
• /
• 2014

Transmission electron microscope (TEM) is an excellent tool for studying the structure and properties of nanostructured materials. As the development of $C_s$-corrected TEM, the direct analysis of atomic structures of nanostructured materials can be performed in the high-resolution transmission electron microscopy (HRTEM). Especially, fast Fourier transform (FFT) technique in image processing is very useful way to determine the crystal structure of HRTEM images in reciprocal space. To apply FFT technique in HRTEM analysis in more reasonable and friendly manner, we made a new circular region of interest (C-ROI) FFT script and tested it for several HRTEM analysis. Consequentially, it was proved that the new FFT application shows more quantitative and clearer results than conventional FFT script by removing the streaky artifacts in FFT pattern images. Finally, it is expected that the new FFT script gives great advantages for quantitative interpretation of HRTEM images of many nanostructured materials.

• Journal of Animal Environmental Science
• /
• v.7 no.2
• /
• pp.103-110
• /
• 2001

This study conducted to monitor decomposition process of the charomatophore pollution solution of an animal slurry by using a CCD camera. After the solution was put into test tube, the images(R, G, B, H, L, S) values of the solution were measured by the imgae processing system, and those of it\`s optical density were measured for three hours to be decomposed by microscopic organism. The values of measured for three hours to be decomposed by microscopic organism. The values of measured images(R, G, B, H, L, S) were analysed and compared with those of the optical density. Some of the results are as follows. 1. High correlation coefficients, which analyzed by using data on linear equations, were 0.9557 and 0.9672. They were decreased regularly in this R-value experiment of RGB level. The microscopic organism in this experiment was effective for decomposition of the red charomatophore pollution solution. 2. The values of all correlation coefficients from relationship between RGB-value and optical density were more than 0.95 except H-values. RGB-values, which were average values of summed R, G, B values, had correlation coefficients of 0.9863, 0.9937. These results showed so good relationship that decomposition process of charomatophore pollution solution could be monitored by a image processing system.