• 한국안광학회지
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• 제18권2호
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• pp.93-99
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• 2013

목적: 본 연구는 현장현시 건성안 진단 테스트 중에 현재 사용되고 있으나 부정확한 결과로 신뢰도가 떨어지는 쉬르머 용지를 대체 할 수 있는 차세대 건성안 진단 테스트 용지를 미세유체공학을 이용하여 개발하고자 하였다. 방법: 왁스로 패턴을 제조한 친수성 크로마토그래피 용지를 pH에 따른 색 변화가 나타나도록 안토시안으로 염색을 하였다. 인공 누액의 젖음 속도를 인공 누액과 32명의 참가자의 눈물을 이용하여 임상 측정하였다. 결과: 인공 누액을 이용하였을 경우 쉬르머 용지에서는 소량의 용액은(0.5 ml이하) 흡수거리가 도출되지 않았으나 새로 개발된 용지는 시간에 따른 인공 누액의 흡수거리가 확연히 나타났다. 임상실험에서도 새로 개발된 검사 용지는 TBUT (tear break-up time)결과와 부합하는 건성안 진단 결과를 보였다. 결론: 개발된 건성안 진단 용지는 간편하게 사용될 수 있을 뿐만 아니라 기존 쉬르머 용지와 같은 현장현시 건성안 진단 매체와 비교 했을 경우 건성안 판별의 정확성이 높았다.

• Biomolecules & Therapeutics
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• 제22권4호
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• pp.355-362
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• 2014

We have developed a fully automated high throughput drug screening (HTDS) system based on the microfluidic cell culture array to perform combinational chemotherapy. This system has 64 individually addressable cell culture chambers where the sequential combinatorial concentrations of two different drugs can be generated by two microfluidic diffusive mixers. Each diffusive mixer has two integrated micropumps connected to the media and the drug reservoirs respectively for generating the desired combination without the need for any extra equipment to perfuse the solution such as syringe pumps. The cell array is periodically exposed to the drug combination with the programmed LabVIEW system during a couple of days without extra handling after seeding the cells into the microfluidic device and also, this device does not require the continuous generation of solutions compared to the previous systems. Therefore, the total amount of drug being consumed per experiment is less than a few hundred micro liters in each reservoir. The utility of this system is demonstrated through investigating the viability of the prostate cancer PC3 cell line with the combinational treatments of curcumin and tumor necrosis factor-alpha related apoptosis inducing ligand (TRAIL). Our results suggest that the system can be used for screening and optimizing drug combination with a small amount of reagent for combinatorial chemotherapy against cancer cells.

• 한국전기전자재료학회논문지
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• 제35권4호
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• pp.342-347
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• 2022

In-situ analyzation and detection of real-time chemical reactions can be a significant part in interpreting the underlying mechanism in very reactive chemical reactions. To do this, first we have designed a microfluidic device (MFD) pattern for observation of synthesis of hierarchical nanostructures based on graphene oxide (GO), conjugating the well-known coupling reaction by which the solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-mediated coupling is enhanced in the presence of n-hydroxysuccinimide (NHS) to make amide bonding, hereafter called as the EDC coupling. Then, we have manufactured microfluidic devices with multiple tens of micrometer-sized channels that can circulate those nanomaterials to be chemically reacted in the channels. These microfluidic devices were made by negative photo lithography and soft lithography. We showed the possibility of using Raman spectroscopy to reveal the basic mechanism of the energy storage applications.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2013년도 춘계학술대회 논문집
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• pp.161-162
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• 2013

• 한국가시화정보학회지
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• 제19권2호
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• pp.41-47
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• 2021

River and stream are the important water supply source in our lives. Eutrophication causes excessive green algae growth including microcystis, which makes harmful to ecosystem and human health. Therefore, the water purification process to remove green algae is essential. In Korea, green algae alarm system exists depending on the concentration of green algae cells in river or stream. To maintain the growth amount under control, green algae monitoring system is being used. However, the unmanned, small and automatic monitoring system would be preferable. In this study, we developed the 3D printed device to measure the concentration of green algae cell using microfluidic droplet generator and deep learning. Deep learning network was trained by using transfer learning through pre-trained deep learning network. This newly developed microfluidic cell counter has sufficient accuracy to be possibly applicable to green algae alarm system.

• KSBB Journal
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• 제25권4호
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• pp.344-350
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• 2010

This paper demonstrates a microfluidic chip incorporating patterned hollow silica beads that can be effectively used for chemotaxis assay. The hollow silica bead has been exploited to develop a carrier for chemoattractant to induce cell migration. The microfluidic chip contains a patterned array of microfabricated docks which can hold only one bead per docking site. The hollow bead placed inside microfluidic chip releases chemotactic reagent (PDGF-BB) around its periphery in a controlled fashion which generates a signal for chemotatic migration of fibroblast cells. The number of cells migrated close to each bead has been assessed. On-chip cell migration assay showed a remarkable result proving the high efficiency and reliable accuracy in quantitative analysis. Therefore, the device could be extensively used in cell migration assay and other various studies related to cellular movements.

• 센서학회지
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• 제18권1호
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• pp.42-47
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• 2009

In this paper we propose a microfluidic chip that measures the mechanical stiffness of cell membrane using impedance measurement. The microfluidic chip is composed of PDMS channel and a glass substrate with electrode. The proposed device uses patch-clamp technique to capture and deform a target cell and measures impedance of deformed cells. We demonstrated that the impedance increased after the membrane stretched and blocked the channel.

• Korea-Australia Rheology Journal
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• 제19권2호
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• pp.61-66
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• 2007

The aggregation characteristics of red blood cells (RBCs) are known as important factors in the microvascular flow system, and increased RBC aggregation has been observed in various pathological diseases, such as thrombosis and myocardial infarction. This paper describes a simple microfluidic device for measuring the RBC aggregation by integrating a microfluidic slit rheometry and laser-backscattering technique. While a decreasing-pressure mechanism was applied to the microfluidic rheometry, a syllectogram (the light intensity versus time) showed an initial increase and a peak caused by the high shear stress-induced disaggregation, immediately followed by a decrease in the light intensity due to RBC aggregation. The critical shear stress (CST) corresponding to the peak intensity was examined as a new index of the RBC aggregation characteristics. The CST of RBCs increased with increasing aggregation-dominating protein (fibrinogen) in the blood plasma. The essential feature of this design was the combination of the rheometric-optic characterization of RBC aggregation with a microfluidic chip, which may potentially allow cell aggregation measurements to be easily carried out in a clinical setting.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제55권2호
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• pp.88-92
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• 2006

We present a microfluidic system with microvalves and a micropump that are easily integrated on the same substrate using the same fabrication process. The fabricated microfluidic system is suitable for use as a disposable device and its characteristics are optimized for use as a micro chemical analysis system (micro-TAS) and lab-on-a-chip. The system is realized by means of a polydimethylsiloxane (PDMS)-glass chip and an indium tin oxide (ITO) heater. We demonstrate the integration of the micropump and microvalves using a new thermopneumatic-actuated PDMS-based microfluidic system. A maximum pumping rate of about 730 nl/min is observed at. a duty ratio of 1 $\%$ and a frequency of 2 Hz with a fixed power of 500 mW. The measured power at flow cut-off is 500 mW for the microvalve whose channel width, depth and membrane thickness were 400 $\mu$m, 110 $\mu$m, and 320 $\mu$m, respectively.

• 청정기술
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• 제25권4호
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• pp.283-288
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• 2019

단분산성 마이크로입자는 약물캡슐화 및 전달을 위한 다양한 응용분야에서 사용되고 있다. 미세유체장치는 매우 균일한 액적을 생산할 수 있는 중요한 장치이며 이 액적은 단분산성 마이크로입자를 생성할 수 있는 중요한 템플레이트(template)로의 역할을 한다. 미세유체장치는 마이크론 크기의 채널로 구성되어 표면장력과 점성력 간의 균형을 정교하게 조절할 수 있으며, 이는 단분산성 액적을 형성하는 필수적인 기술 중의 하나이다. 본 연구는 유동집적채널 기반의 미세유체장치에서 매우 균일한 polycaprolactone (PCL) 생분해성 고분자 입자를 제조하는 방법을 제안한다. 유동집적채널 기반의 미세유체장치는 polydimethylsiloxane (PDMS) 기반의 소프트리소그래피(soft-lithography) 방법을 통해 제작된다. 액적 생성에서 중요한 요소는 마이크로 액적의 크기와 단분산성을 조절하는 것이다. 이를 위해, 본 연구에서는 이 미세유체장치에서 오일용액 분산상과 수용액 연속상의 부피유속을 제어하여 단분산성 액적 형성 조건을 최적화하였다. 그 결과 균일한 액적을 형성할 수 있는 dripping 영역에 대한 최척화된 유속조건을 확인하였다. 그런 다음, 마이크로입자를 생성하기 위해 PCL 고분자를 포함한 액적을 장치에서 형성한 후 용매의 증발에 의해 입자화 하였다. 입자의 크기는 부피유속과 미세유체채널의 크기에 의해 조절되며 입자의 단분산도는 변동계수(coefficient of variation, CV)값이 5% 이하로 제어될 수 있다.