KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Chemotactic Cell Migration around Hollow Silica Beads Containing Chemotatic Reagent

약물 담지 다공성 중공 실리카 미세구 주위 세포의 주화성 이동

  • Kim, Hae-Chun (Department of Chemistry, College of Natural Sciences, Kongju National University) ;
  • Kang, Mi-Seon (Department of Chemistry, College of Natural Sciences, Kongju National University) ;
  • Rhee, Seog-Woo (Department of Chemistry, College of Natural Sciences, Kongju National University)
  • 김해춘 (공주대학교 자연과학대학 화학과) ;
  • 강미선 (공주대학교 자연과학대학 화학과) ;
  • 이석우 (공주대학교 자연과학대학 화학과)
  • Received : 2010.06.07
  • Accepted : 2010.08.17
  • Published : 2010.08.31
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Abstract

This paper demonstrates a microfluidic chip incorporating patterned hollow silica beads that can be effectively used for chemotaxis assay. The hollow silica bead has been exploited to develop a carrier for chemoattractant to induce cell migration. The microfluidic chip contains a patterned array of microfabricated docks which can hold only one bead per docking site. The hollow bead placed inside microfluidic chip releases chemotactic reagent (PDGF-BB) around its periphery in a controlled fashion which generates a signal for chemotatic migration of fibroblast cells. The number of cells migrated close to each bead has been assessed. On-chip cell migration assay showed a remarkable result proving the high efficiency and reliable accuracy in quantitative analysis. Therefore, the device could be extensively used in cell migration assay and other various studies related to cellular movements.

Keywords

References

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