• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.1
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• pp.70-74
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• 2000

Agar for microbiological medium was prepared with pilot-scale for industrial application by the process of microfiltration ($0.4 {\mu}m\;pore size$) in $40{\~}50{\circ}C$, washing with sot water, and treatment with $0.25\;N NaOH\;at\;70{\circ}C$. Transparency, gel strength, viscosity, sulfate content, and syneresis ratio of agar prepared from Gelidium amansii was compared with commercial agar for microbiological medium. Gel strength and transparency were increased with processing, however, it's viscosity, sulfate content, and syneresis ratio were reduced. The final agar product was superior to commercial agar for microbiological medium.

• The Microorganisms and Industry
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• v.26 no.2
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• pp.2-12
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• 2000

• Korean Journal of Microbiology
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• v.27 no.3
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• pp.310-315
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• 1989

Tabtoxin produced in Pseudomonas syringae pv. tabace irreversibly inhibits its known physiological target, glutamine synthetase so that causes wildfire disease on leaves of host plant. In this study, we examined a rapid and sensitive microbiological method for tabtoxin assay in several media. In minimal A agar medium nd minimal glucose agar medium, growth inhibition zone of Agrobacterium tumefaciens was larger than that of other indicator strain. However, mostly, growth inhibition zone of indicator strains on the minimal glucose agar medium was smaller than that of on the miniaml A agar medium. In complex agar medium, growth inhibithiton zone was not observed in all the tested indicator strains. Pseudomonas syringae pv. tabaci produced more tabtoxin according to the incubation time. When glutamine was added to the minimal glucose agar medium, growth inhkbition zone of Agrobacterium tumefaciens was reduced.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.222.2-222.2
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• 2002

• Proceedings of the Microbiological Society of Korea Conference
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• 1998.04a
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• pp.45.2-45.2
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• 1998

• Biomedical Science Letters
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• v.29 no.4
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• pp.249-255
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• 2023

Transporting clinical samples for microbiological testing requires a proper transport medium that guarantees the survival of microorganisms. Therefore, the aim of the study was to determine the ability of Amies Transport Medium (ATM) to maintain the viability of microorganisms in clinical specimens and its suitability as a transport medium for microbiological testing. This study evaluated the performance of swab provided by KS Co., Ltd. for three groups of bacteria comprising aerobic and facultative anaerobic bacteria, anaerobic bacteria, and fastidious bacteria, according to the Clinical and Laboratory Standard Institute (CLSI) 8.11.2. The ATM stability test was conducted by dividing the medium into two groups based on the product expiration date of use. All tested media, A and B (the date of manufacture and expiration date are different) showed ≥5 CFU, and there was no significant difference in the result values of Category A and Category B with different serial numbers for each test. The results of this experiment when cross-checked with the guidelines suggest that ATM is a suitable transport medium for microbiological testing, as it maintains the viability of microorganisms and is suitable for overgrowth trials. In addition, compared to the number of CFUs at the origin, the number of CFUs did not increase by more than 1 log after storage. These results have important implications for the development of transport media that can guarantee the survival of microorganisms in clinical specimens.

• The Korean Journal of Mycology
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• v.44 no.2
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• pp.87-93
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• 2016

From 182 non-pathogenic wild yeast isolates from flowers, Pichia silvicola UL6-1 and Sporobolomyces carnicolor 402-JB-1 were selected for potent ${\gamma}$-aminobutyric acid production and microbiological characteristics were investigated. Pichia silvicola UL6-1 formed ascospores and pseudomycelia. The strain was also halotolerant, growing well in 5% NaCl-containing yeast extract-peptone-dextrose (YPD) medium. Sporobolomyces carnicolor 402-JB-1 did not form ascospores or pseudomycelia and grew well on 10% glucose-yeast extract-peptone medium.

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.196.1-196
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• 2005

• The Microorganisms and Industry
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• v.19 no.3
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• pp.41-45
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• 1993

The cultural conditions of the osmophilic red color yeasts (Strain L$_{1}$, L$_{2}$, L$_{3}$ and L$_{4}$) isolated and identified in the previous report were examined and the results obtained were as follows ; 1. The optimum medium for growth of these osmophilic red color yeasts was soy sauce medium. 2. These strains were grown exceedingly well on the medium containing 3 percent of NaCl but somewhat restrained on the medium containing 6% or more. 3. The optimum temperautre for growth of these strains was 25.deg.C and their lethal temperature was 68.deg.C(treatment for 5 minutes). 4. The optimum pH for growth of these strains was 6.0.

• Korean Journal of Microbiology
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• v.29 no.5
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• pp.267-269
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• 1991

The hyphal tip laccase of Coprinus congregatus which is a membrane-associated enzyme and shows diffdrdnt banding patterns of PAGE analysis when compared with the enzyme of liquid culture (Choi et al. 1987) has been successfully secreted to culture medium in liquid shake culture by lowering the pH of medium to 4.0. When the fungus is cultivated in YpSs(pH 4.0) liquid, only the hyphal tip laccase is found in the medium after 6 hr incubation and there is no liquid-type enzyme when examined by PAGE analysis.