The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Screening of γ-Aminobutyric Acid (GABA)-Producing Wild Yeasts and their Microbiological Characteristics

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Jeon, Sun-Jeong (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Hyang-Burm (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • Received : 2016.02.29
  • Accepted : 2016.06.13
  • Published : 2016.06.30
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Abstract

From 182 non-pathogenic wild yeast isolates from flowers, Pichia silvicola UL6-1 and Sporobolomyces carnicolor 402-JB-1 were selected for potent ${\gamma}$-aminobutyric acid production and microbiological characteristics were investigated. Pichia silvicola UL6-1 formed ascospores and pseudomycelia. The strain was also halotolerant, growing well in 5% NaCl-containing yeast extract-peptone-dextrose (YPD) medium. Sporobolomyces carnicolor 402-JB-1 did not form ascospores or pseudomycelia and grew well on 10% glucose-yeast extract-peptone medium.

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References

  1. Yi SH, Ann YG, Choi JS, Lee JS. Development of peach fermented wine. Kor J Food Nutr 1996;9:409-12.
  2. Lee TS, Lee SJ. Studies on the yeasts for the brewing of soy sauce (I): isolation, identification and classification of the yeasts in the soy sauce koji. J Kor Agric Chem Soc 1970;13:97-103.
  3. Kim JH, Kim NM, Lee JS. Physiological characteristics and ethanol fermentation of thermotolerant yeast Saccharomyces cerevisiae OE-16 from traditional Meju. Kor J Food Nutr 1999;12:490-5.
  4. Min JH, Ryu JJ, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Beakamsan of Korea. Kor J Mycol 2013;41:47-51. https://doi.org/10.4489/KJM.2013.41.1.47
  5. Hyun SH, Min JH, Kim SA, Lee JS, Kim HK. Yeasts associated with fruits and blossoms collected from Hanbat arboretum, Daejeon, Korea. Kor J Mycol 2014;42:178-82. https://doi.org/10.4489/KJM.2014.42.2.178
  6. Hyun SH, Lee JG, Park WJ, Kim HK, Lee JS. Isolation and diversity of yeasts from fruits and flowers of orchard in Sinammyeon of Yesan-gun, Chungcheongnam-do, Korea. Kor J Mycol 2014;42:21-7. https://doi.org/10.4489/KJM.2014.42.1.21
  7. Hyun SH, Han SM, Lee JS. Isolation and physiological functionality of yeasts from wild flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea. Kor J Mycol 2014;42:201-6. https://doi.org/10.4489/KJM.2014.42.3.201
  8. Hyun SH, Han SM, Kim HK, Lee JS. Yeasts diversity of wild flowers in mountains of Korea and their physiological functionalities. Kor J Mycol 2015;43:137-41.
  9. Hayakawa K, Kimura M, Kasaha K, Matsumoto K, Sansawa H, Yamori Y. Effect of a gamma-aminobutyric acid-enriched dairy product on the blood pressure of spontaneously hypertensive and normotensive Wistar-Kyoto rats. Br J Nutr 2004;92:411-7. https://doi.org/10.1079/BJN20041221
  10. Inoue K, Shirai T, Ochiai H, Kasao M, Hayakawa K, Kimura M, Sansawa H. Blood-pressure-lowering effect of a novel fermented milk containing gamma-aminobutyric acid (GABA) in mild hypertensives. Eur J Clin Nutr 2003;57:490-5. https://doi.org/10.1038/sj.ejcn.1601555
  11. Jakobs C, Jaeken J, Gibson KM. Inherited disorders of GABA metabolism. J Inherit Metab Dis 1993;16:704-15. https://doi.org/10.1007/BF00711902
  12. Wong CG, Bottiglieri T, Snead OC 3rd. GABA, gammahydroxybutyric acid, and neurological disease. Ann Neurol 2003;54:3-12.
  13. Ueno H. Enzymatic and structural aspects on glutamate decarboxylase. J Mol Catal B Enzym 2000;10:67-79. https://doi.org/10.1016/S1381-1177(00)00114-4
  14. Kim JH. Isolation and characterization of high GABA producing yeast isolated from fermented Bokbunja wine [dissertation]. Jeonju (Korea): University of Chonbuk; 2014.
  15. Lee SH. Study on a gamma-aminobutyric acid (GABA) producing yeast isolated from Korean fermented soybean product, meju and optimal condition for GABA synthesis [dissertation]. Jeonju (Korea): University of Chonbuk; 2014.
  16. Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33. https://doi.org/10.4489/KJM.2014.42.1.28
  17. Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Kor J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  18. Han SM, Hyun SH, Lee JS. Isolation and identification of yeasts from wild flowers in Deogyu mountain and their physiological functionalities. Kor J Mycol 2015;43:47-52. https://doi.org/10.4489/KJM.2015.43.1.47
  19. Holdiness MR. Chromatographic analysis of glutamic acid decarboxylase in biological samples. J Chromatogr 1983;277:1-24. https://doi.org/10.1016/S0378-4347(00)84819-7
  20. Han SM, Hyun SH, Lee HB, Lee HW, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers collected around Jangseong lake in Jeollanam-do, Republic of Korea, and characterization of the unrecorded yeast Bullera coprosmaensis. Mycobiology 2015;43:266-71. https://doi.org/10.5941/MYCO.2015.43.3.266
  21. Jeong SC, Hyun KW, Kim JH, Lee JS. Isolation of halotolerant yeast and the production of extracellular protease. Kor J Biotechnol Bioeng 2001;16:158-62.
  22. Kang KH, Bae IH, Lee CH. Studies on extracellular protease from Saccharomyces lipolytica: conditions of enzyme production. Kor J Appl Microbiol Biotechnol 1987;15:279-85.
  23. Lee JS, Yi SH, Kwon SJ, Ahn C, Yoo JY. Enzyme activities and physiological functionality of yeasts from traditional Meju. Kor J Appl Microbiol Biotechnol 1997;25:448-53.