• Title/Summary/Keyword: microbiological characteristics

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Role of dendritic cells in infectious disease and tumor (감염질환(infectious disease)과 종양면역(tumor immunity)에 있어서 수지상 돌기세포(dendritic cells)의 역할)

  • 임종석;김광동
    • The Microorganisms and Industry
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    • v.26 no.1
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    • pp.8-17
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    • 2000
  • Thirtythree strains of the Aspergillus spp. isolated from foodstuffs were observed through some physiological characteristics for detection of identification key of Aspergillus spp. 1) Each strain of Aspergillus spp. had their specific characteristics and could be used for identification of species. 2) Excellent amylase-producing fungi were observed among the isolated strains of Aspergillus spp. 3) Amylase activities increased for one week incubation period. 4) In the tests of common characters of aflatoxin-producing fungi among the 33 strains of Aspergillus spp., for example, conidial size, presence of sclerotia, kojic acid, and pigment production, coloration of phenol, reduction of methylene blue, etc.

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Effects of Staphylococcus carnosus on Quality Characteristics of Sucuk (Turkish Dry-Fermented Sausage) During Ripening

  • Kaban, Guzin;Kaya, Mukerrem
    • Food Science and Biotechnology
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    • v.18 no.1
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    • pp.150-156
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    • 2009
  • In this study, Staphylococcus carnosus isolated from traditional sucuk (Turkish dry-fermented sausage) was used in combination with Lactobacillus plantarum as a lactic culture in sucuk production. Sucuk produced with only L. plantarum was evaluated as a control group. Microbiological, physicochemical, and volatile profile characteristics of sucuk samples were investigated during ripening. In both sausages with S. carnosus and control group, pH value decreased to below 5.0 at the $3^{rd}$ day. In all samples, Aw value decreased as the ripening time progressed. Sausages with S. carnosus showed the higher nonprotein nitrogen (NPN) value than control group. However, the highest mean value for thiobarbituric acid reactive substances (TBARS) was observed in control group. Enterobacteriaceae dropped to undetectable levels at the $3^{rd}$ day in both groups. S. carnosus increased approximately 1 log unit within the first 3 days of the fermentation. In the presence of S. carnosus, significant changes were observed in only a few volatile compounds.

Changes in Fermentation Characteristics of Kimchi Added with Leek (부추첨가 김치의 발효특성 변화)

  • 이귀주;김유경
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.4
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    • pp.780-785
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    • 1999
  • The effect of addition in different amounts of leek(4, 8, 12% respectively) during fermentation of kimchi was investigated. Fermentation characteristics such as pH, acidity and total reducing sugars as well as microbiological properties were determined. During fermentation, pH was more slowly lowered in kimchi added with leek than in control and titrable acidity of these kimchi was lower than that of control. Viable cells of total bacteria and lactic acid bacteria in these kimchi were higher than that of control during fermentation. Content of total reducing sugars was higher than that of control. Three kinds of reducing sugars such as fructose, glucose and galactose were detected and the dominant one appeared to be fructose. These results suggested that addition of leek seems to retard fermentation of kimchi due to their anti microbial actvity.

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Characteristics and Numerical Taxonomy of Aeromonas spp. Isolated from Naktong Estuary (낙동강 하구에서 분리한 Aeromonas spp.의 수리학적 분류 및 특성)

  • 전도용;하영칠
    • Korean Journal of Microbiology
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    • v.27 no.4
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    • pp.385-390
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    • 1989
  • One hundred and sixty one strains of Aeromonas were isolated at three sites from August, 1986 to December, 1986 in Naktong Estuary. Cluster analysis was performed on total of 42 morphological and biochemical characteristics of the isolated strains. At the level of 85% and 84% similarity, three major clusters and two minor clusters had been identified: the first three clusters were different one another from MR reaction and gas production utilizing glucose. The five clusters were classified as two serotypes on the basis of serotyping, which was consistent with the result of cytotoxicity test.

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Phenotypic characteristics and antimicrobial susceptibility of verotoxin -producing E coli from slaughtered cattle

  • Byun Jae-Won;Kim Kyoung-Ho;Lee Sung-Mo;Hwang Hyun-Soon;Kim Yong-Hee
    • Korean Journal of Veterinary Service
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    • v.28 no.4
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    • pp.407-412
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    • 2005
  • Ten isolates of Verotoxin-producing Escherichia coli (VTEC) were detected in slaughtered cattle and investigated their phenotypic characteristics and antimicrobial susceptibility. None of the isolates was positive for eae gene. Only one isolate was positive for uidA gene. Eight out of ten isolates of VTEC were originated from broker's cattle. Thus microbiological monitoring for broker farms should be performed to minimize VTEC contamination. In the antimicrobial susceptibility test, all the isolates were highly resistant to bacitracin and lincomycin whilst they are susceptible to apramycin and neomycin.

Fermentation Characteristics of Low-Salt Kimchi with Starters on Fermentation Temperature and Salt Concentration (종균이 첨가된 저염김치의 발효온도 및 소금 농도에 따른 발효 특성)

  • Moon, Sung-Won;Park, Sun-Hyun;Kang, Byung-Sun;Lee, Myung-Ki
    • The Korean Journal of Food And Nutrition
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    • v.27 no.5
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    • pp.785-795
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    • 2014
  • This study was conducted to develop low-salinity Kimchi with acetic acid, starters and fruits added for health and taste. The quality characteristics of the developed Kimchi by storage period and salt concentrations were investigated herein. The effects of salinity and fermentation temperature ($0^{\circ}C$, $5^{\circ}C$) on the quality characteristics of low-salinity Kimchi were investigated through physicochemical and microbiological analysis. The sensory characteristics are shown in the results below. The pH and acidity were quickly changed by higher temperature over longer storage periods, with gradual decrease in the pH accompanied with an increase in acidity. The microbiological analysis revealed increase in the total cell number regardless of the concentration and strain added during the storage period. Finally, test of the overall acceptability with regard to sensory characteristics according to the different strain sets in low-salinity Kimchi showed the average score for smell, salty taste and overall acceptability were highest in the hetero-fermentation strain group.

On the Cultural Characteristics and Wood-Decayedness of Wood-Decaying Fungi in Korea (목재부후균의 배양적 특성과 부후성에 관한 연구)

  • 윤정구;홍순우;백수봉
    • Korean Journal of Microbiology
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    • v.19 no.3
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    • pp.128-136
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    • 1981
  • In order to investigate early identification of species of the wood-decaying fungi in the the mycelial stage, the authors isolated of 41 species, 28 genus, 9 family at 8 locations in Korea and cultivated these isolates on the various kinds of solid media. After investigating such cultural characteristics as oxidase reactions with tannic and gallic acid, various morphological features of colony and growth grade, appeared on the various media, the authors obtained the following results : 1. The oxidase reactions with tannic and gallic acid in the PDTA, DTA, PDGA and DGA media are available for identificantion of the wood-decaying fungi. 2. The oxidase reactions with guaiacol, pyrogallol and hydroquinone in the PDGUA, PDPA and PDHA media are not so much available for identification of the wood-decaying fungi. 3. Morphological features of colonies such as mycelium color, floccose, floccose-powdery, mycelloid, powdery-mycelloid, velvet, radiate, contoured, rosulate and growth grade on the PDA, PSA and PXA media are useful for identification of wood-decaying fungi. 4. It is believed that early identification in species level of wood-decaying fungi using cultural characteristics in the mycelial stage is possible. 5. The key for the identification of 41 species of wood-decaying fungi is proposed by the cultural characteristics using several solid media.

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A Study on the Quality Characteristics of Brand and Non-brand Korean beefs (국내산 브랜드 한우와 비브랜드 한우의 품질 특성 연구)

  • Kim, Mi-Hyun;Song, Hyo-Nam;Rho, Jeong-Hae
    • Korean journal of food and cookery science
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    • v.23 no.2 s.98
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    • pp.187-194
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    • 2007
  • The quality characteristics of brand and non-brand Korean beefs, found in retail circulation, were studied via physicochemical and microbiological analysis and sensory tests. The average price of 100 g of brand Korean beef sirloin was 7.6 USD (1USD=950won), while the price of non-brand Korean beef was 3.1 USD. The fat content of the brand beef seemed to be higher, yet the difference was not significant. In both the brand and non-brand beefs, pH levels were approximately 5.27 We found that lightness (Hunter L value) tended to be higher in the brand beef, but again, this difference was not significant. For the cooked beef texture, the brand beef seemed to have lower gumminess, and had significantly less fracturability, than the non-brand beef. Significant differences could not be found with regards to microbiological stability between the two types of beef. In the raw beef sensory test, significant differences were identified for meat color, fat color, fat in muscle, flavor, and general preference. Also, the after cooking sensory test showed that brand beef had better characteristics for color, flavor, juiciness, softness, taste, chewiness, and preference.

Quality Characteristics of Grapes during Post-irradiation Storage at Different Temperatures (감마선 조사 포도의 저장온도에 따른 품질특성 변화)

  • Yun, Hye-Jeong;Joe, Min-Ho;Kwon, Joong-Ho;Lim, Byung-Lak;Kim, Dong-Ho
    • Food Science and Preservation
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    • v.15 no.5
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    • pp.648-655
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    • 2008
  • A gamma irradiation process was applied to prolong the shelf-life of grapes and changes in microbiological, physicochemical, nutritional, and sensory characteristics were investigated during 6 weeks of post-irradiation storage at $4^{\circ}C$ and $25^{\circ}C$. Physiochemical characteristics of grapes such as total sugar content, pH, titratable acidity, and anthocyanine content, were stable after 1 kGy of irradiation, a dose recommended by CODEX for fruits and vegetables. The viable cell counts of contaminating microorganisms were reduced 1-2 logs by this radiation dose. After 6 weeks of storage at $4^{\circ}C$ the microbiological quality of irradiated grapes was stable, but all non-irradiated grapes had deteriorated. Whether or not grapes were irradiated, weight loss rapidly increased after 2 weeks of storage at $25^{\circ}C$, whereas weight loss was delayed by storage at $4^{\circ}C$. Immediately after gamma irradiation, the sensory evaluation results did not differ between samples, and the sensory quality of the irradiated grapes remained acceptable for over 6 weeks of storage at $4^{\circ}C$. The results suggest that 1 kGy of gamma irradiation does not detrimentally affect the nutritional or physical characteristics of grapes, especially when cold storage follows radiation treatment. In conclusion, a combination of gamma irradiation and cold storage prolonged the shelf-life of grapes by 6 weeks.

Microbiological Characteristics of Wild Yeast Strain Pichia anomala Y197-13 for Brewing Makgeolli

  • Kim, Hye Ryun;Kim, Jae-Ho;Bai, Dong-Hoon;Ahn, Byung Hak
    • Mycobiology
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    • v.41 no.3
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    • pp.139-144
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    • 2013
  • Makgeolli is a traditional cloudy-white Korean rice wine with an alcohol content of 6~7%. The present study investigated the morphological characteristics, carbon-utilizing ability, fatty acid composition, alcohol resistance, glucose tolerance, and flocculence of Saccharomyces cerevisiae Y98-5 and Pichia anomala Y197-13, non-S. cerevisiae isolated from Nuruk, which is used in brewing Makgeolli. Similar morphological characteristics were observed for both isolated wild yeast strains; and the carbon source assimilation of Y197-13 differed from that of other P. anomala strains. Strain Y197-13 was negative for D-trehalose, mannitol, arbutin, I-erythritol, and succinic acid. The major cellular fatty acids of strain Y197-13 included C18:2n6c (33.94%), C18:1n9c (26.97%) and C16:0 (20.57%). Strain Y197-13 was Crabtree-negative, with 60% cell viability at 12% (v/v) ethanol. The flocculation level of strain Y197-13 was 8.38%, resulting in its classification as a non-flocculent yeast.