• Food Science of Animal Resources
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• v.37 no.2
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• pp.313-319
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• 2017

This study was conducted to develop calcium-fortified shank bone extract (SBE) and to determine the effect of adding calcium lactate on physico-chemical characteristics of SBE during cold storage. The following five experiment groups were used: Control (0%, no addition), T1 (0.05% calcium lactate), T2 (0.1% calcium lactate), T3 (0.5% calcium lactate), and T4 (1% calcium lactate). When the concentration of calcium lactate added to the SBE was increased, the pH, redness, and yellowness values were significantly reduced, whereas the salinity, sugar content, and turbidity of SBE were significantly increased. Sensory parameters such as aroma, flavor, and overall acceptability in the control, T1, and T2 had similar scores. The TBARS values of SBE was significantly increased when 1% of calcium lactate was added, and the VBN values of SBE with calcium lactate at day 7 were higher than that of control (p<0.05). However, the addition of calcium lactate showed an inhibition effect on the growth of total microbial counts in SBE until 4 d of storage. The calcium content of SBE was increased by the addition of calcium lactate in a dose-dependently manner. The proper addition level of calcium lactate in the SBE was determined to be 0.1%.

• Applied Biological Chemistry
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• v.46 no.1
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• pp.12-15
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• 2003

The effect of chlorella addition on quality and shelf-life of soybean curd was investigated. The yield of chlorella soybean curd was the highest with 1.0% (w/w) chlorella addition. Hardness, gumminess, and chewiness of chlorella soybean curd increased, whereas Hunters color values decreased in proportion to amount of chlorella added. No significant differences were observed between the sensory properties of chlorella and ordinary soybean curds. Microbial counts of soybean curd stored in sterilized distilled water as tofu-immersion solution increased, whereas pH decreased during storage at $15^{\circ}C$. After 5 days storage, microbial counts of the soybean curds containing 0.5 and 1.O% chlorella were lower than that of the ordinary soybean curd. These results imply that chlorella is a useful additive to suppress the proliferation of aerobic microorganism in soybean curd at the optimal concentration of chlorella around 1%.

• Korean journal of food and cookery science
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• v.14 no.2
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• pp.195-200
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• 1998

Wheat flour and glutinous rice pastes added Kimchi were fermented at 10$^{\circ}C$ for 14 days. The numbers of total viable microorganisms and lactics were counted. Textural property by compression force and relevant changes in pectic composition were determined. Other physical properties such as color and viscosity of Kimchi juices were evaluated. Regarding the microorganism counts, the total cell counts of all Kimchi samples increased until 5th days but thereafter all decreased during fermentation. The number of lactics showed same tendency in all Kimchi samples. The compression force of control, wheat flour paste added Kimchi (WHFP-Kimchi) and glutinous rice paste added Kimchi (GLRP-Kimchi) decreased up to 72.0%, 77.0%, 66.5% respectively. During fermentation, hot water soluble pectin (HWSP) increased, whereas sodium hexametaphosphate soluble pectin (HXSP) and HCI soluble pectin (HCISP) decreased. The red chromaticity of all Kimchi juices decreased. The viscosity of all Kimchi samples decreased in the order of GLRP-Kimchi, WHFP-Kimchi and control.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.3
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• pp.364-368
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• 2011

An experiment was conducted to examine the effects of Cordyceps militaris mycelia on microbial populations and fibrolytic enzyme activities in vitro. C. militaris mycelia was added to buffered rumen fluid with final concentrations of 0.00, 0.10, 0.15, 0.20, 0.25 and 0.30 g/L and incubation times were for 3, 6, 9, 12, 24, 36, 48 and 72 h. At all incubation times, the supplementation of C. militaris mycelia linearly increased the number of total viable and celluloytic bacteria; maximum responses were seen with 0.25 g/L supplementation of C. militaris mycelia. The addition of C. militaris mycelia above the level of 0.20 g/L significantly (p<0.01) increased the number of total and cellulolytic bacteria compared with the control. On the other hand, the response of fungal counts to the supplementation of C. militaris mycelia showed a linear decrease; the lowest response was seen with 0.30 g/L supplementation of C. militaris mycelia. It would seem that C. militaris mycelia possess a strong negative effect on rumen fungi since the lowest level of C. militaris mycelia supplementation markedly decreased fungal counts. Carboxylmethyl cellulase activities were linearly increased by the addition of C. militaris mycelia except at 3 and 9 h incubation times. At all incubation times, the supplementation of C. militaris mycelia linearly increased the activities of xylanase and avicelase. In conclusion, the supplementation of C. militaris mycelia to the culture of mixed rumen microorganisms showed a positive effect on cellulolytic bacteria and cellulolytic enzyme activities but a negative effect on fungi.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.4
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• pp.592-596
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• 2000

This study was carried out investigate the effect of sepiae as on the kimchi ferentation. Quality indices were pH, titratable acidity, reducing sugar content, microbial counts, and redness kimchi juice. Sepiae as retarded the decreasing rate of pH and the increasing rate of titratable acidity in kimchi during fermentation at 1$0^{\circ}C$, and the effect was more conspicouous at 1.5% than at 0.5%. Kimchi added with 1.5% sepiae as maintained a similar pH and titratable acidity after 5 days. Microbial counts of total and lactic bacteria were higher in sepiae os added kimchi than control control. Regardless of the of sepoae os, the redness of kimchi juice was gradually increased during storage.

• Journal of the Korean Society of Food Culture
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• v.11 no.4
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• pp.465-473
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• 1996

The purpose of this study was to evaluate microbiological hazards in the steps of production, transportation and selling of hamburger and sandwich that were marketed in CVS, then to identify methods of control. The reasults are as follows: As the reasult of operation surroundings of manufacturerand reserch of circulation, $4{\sim}6$ hours are needed from manufacturer to CVS. Also transportation car mean temperature was $10^{\circ}C$ which exceeds the standard of $7^{\circ}C$ or below. Hamburger: Critical control points identified were purchasing, cooking, post-preparation, transportation and holding at CVS. As the reasult of microbial analysis following the case of holding methods and reheating at CVS, microbes of cold holding and reheat after cold holding was within standard value. But in the case of room temperature microbes exceeded standard value. Sandwich: Critical control points identified were purchasing, cooking, post-preparation, transportation and holding at CVS. As the reasult of microbial analysis following the case of holding methods and reheating at CVS, total plate counts of cold holding and reheat after cold holding was within standard value. But in the case of room temperature holding after 24 hours total plate counts exceeded standard value. In the case of room temperature holding the number of microbes increased according to the passage of time. As a reasult of food poisoning bacteria, it was negative in every test in sample against V. parahaemolyticus, Salmonella, S. aureus.

• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.104-113
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• 2021

This study was conducted to investigate the efficiency of a microencapsulated mixture of organic acids (MOA) with low protein in piglet feed on growth performance, diarrhea score, nutrient digestibility, fecal microbial counts, and blood profiles in weaning pigs. A total of 80 pigs [(Landrace × Yorkshire) × Duroc; 6.8 ± 0.48 kg] were randomly assigned to four dietary treatment groups: high protein (HP); low protein (LP); MOA1, LP + 0.2% MOA; and MOA2, LP + 0.3% MOA. The MOA2 group had higher average daily weight gains (during days 0-14 and days 0-28), diarrhea score (during days 0-14, during days 14-28 and days 0-28) and greater digestibility of dry matter (days 14 and 28) compared to the LP group (p < 0.05). However, there were no significant differences (p > 0.05) between the pigs fed diets with the MOA1 and MOA2 in blood profiles and fecal microflora. In conclusion, this study indicates that piglets fed 0.3% MOA in low protein diets maintained similar growth performance and nutrient digestibility, but alleviated the incidence of diarrhea compared to piglets fed high protein diets.

• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.965-973
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• 2021

The intention of this research is to analyze the effects of lactulose (LAC) supplementation on the growth performance, nutrient digestibility, microbial shedding, and fecal noxious gas emissions on weaning pigs in a 42-day trial. Based on the initial body weight and sex, a total of 255 piglets (21 day old) were randomly allocated into one of three dietary treatments with 15 replications and five pigs (two female and three male) per pen. The dietary treatments were as follows: a corn-soybean meal-based basal diet (CON) supplemented with 0, 1, and 2 g·kg^-1 of LAC. During phase 1, significant (p < 0.05) increases in the average daily feed intake and average daily gain (ADG) were observed, whereas during phase 2 and overall experimental period, significant improvements (p < 0.05) in the body weight, ADG, and gain to feed ratio were observed in pigs fed a graded level of LAC compared to those fed the CON diet. Additionally, dietary LAC supplementation significantly improved (p < 0.05) the nutrient digestibility dry matter, nitrogen, and gross energy in both phase 1 and phase 2. Moreover, the inclusion of LAC supplementation significantly increased (p < 0.05) the fecal Lactobacillus counts and reduced (p > 0.05) the E. coli counts in pigs. Furthermore, LAC supplementation reduced (p > 0.05) fecal ammonia and hydrogen sulfide gas emissions during phase 2. The results here indicate that the addition of lactulose at 1 g·kg^-1 and/or 2 g·kg^-1 would be optimal to improve the performance outcomes of weaning piglets.

• Journal of Animal Science and Technology
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• v.49 no.3
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• pp.359-368
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• 2007

Holstein cows(n=12) were assigned to one of three diets containing control diet and diets containing a microbial culture, 50ml(T1) and 100ml(T2) SDN(R)(amicrobial culture), per day. The basal diet containing concentrate mixture, corn silage and timothy hay were fed midlactating cows for 12 weeks. Milk production tended to be higher for cows fed T2 diet (20.8kg/day) than fed T1(19.7kg/d) and control diet (19.2kg/day).  There was a tendency of an increase in 4% FCM for cows fed T2 diet(19.6kg/day) than T1(18.8kg/d) and control diet(18.4kg/day). Milk components were not found to be different between cows fed control diet and SDN(R) diets. There was a tendency an increase in milk protein for cows fed control diet(3.43%) compared with microbial diets, T1 and T2(3.08% and 3.20%). However, milk protein production was not significantly different between control diet(0.65kg/d) and T1(0.61kg/d) or T2(0.67kg/d). Somatic cell counts for cows fed T1(72,000) and T2(60,000/ml) were lower than cows fed control diet (108,000/ml) (P<0.05). In conclusion, the cows that were fed diets containing SDN(R) as a microbial culture resulted a tendency of an increase in milk production and a reduction of somatic cell counts which indicates improved milk quality and hygiene.

• Food Science of Animal Resources
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• v.29 no.1
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• pp.24-33
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• 2009

This study investigated the microbial contamination levels of raw meats used for short-ripened salami and changes in the microbial and physico-chemical properties of the product during storage at 10 and $25^{\circ}C$ for up to 120 days. The microbial counts of raw meats ranged between 2 and 4 Log CFU/g. Frozen-thawed sow meat showed higher total aerobe and Enterobacteriaceae counts than fresh chilled pork and pork back fat. Staphylococcus aureus was found in all raw materials except fresh chilled pork samples, and Clostridium perfringens was detected in a sample stored for 21 days at $25^{\circ}C$. The counts of total aerobes, lactic acid bacteria and Staphylococcus spp. decreased more rapidly at $25^{\circ}C$ than at $10^{\circ}C$ when the storage time was extended. The growth of Enterobacteriaceae, Pseudomonas spp., Clostridium spp., yeast, and mold were restricted to levels below 2 Log CFU/g during storage. The contents of salt, water, crude protein, crude fat, and ash of salami samples were 3.4, 33.4, 30.8, 32.7, and 4.3%, respectively, which were not affected by storage time or temperature. The pH value of the salami was initially 4.79 and increased to 5.02 and 5.26 after 120 days of storage at 10 and $25^{\circ}C$, respectively, whereas the water activity values decreased from an initial value of 0.91 to 0.90 and 0.88 after 120 days at 10 and $25^{\circ}C$, respectively. The TBA and VBN values increased slowly during storage. The redness value of the salami samples stored at $25^{\circ}C$ decreased more significantly than the samples stored at $10^{\circ}C$. With increased storage time, the values for the rheological characteristics of the salami in terms of hardness, brittleness, elasticity, cohesiveness, gumminess, and adhesiveness tended to decrease more remarkably at $25^{\circ}C$ than at $10^{\circ}C$. Based on sensory evaluation scores, it appears that short-ripened salami is no longer acceptable after 90 days at $10^{\circ}C$ and 30 days at $25^{\circ}C$.