• Asian Pacific Journal of Cancer Prevention
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• v.14 no.9
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• pp.5017-5021
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• 2013

Objective: MicroRNAs (miRNAs) are a small class of non-coding, single-stranded RNAs with a critical role in genesis and maintenance of renal cancer mainly through binding to 3'-untranslated regions (3'UTR) of target mRNAs, which causes a block of translation and/or mRNA degradation. The aim of the present study was to investigate the potential effects of miR-122 in human renal cell carcinomas. Methods: The expression level of miR-122 was quantified by qRT-PCR. MTT, colony formation, invasion and migration assays were used to explore the potential functions of miR-122 in human renal cell carcinoma cells. Results: Cellular growth, invasion and migration in two A498 and 786-O cells were significantly increased after miR-122 transfection. Further experiments demonstrated that overexpression of miR-122 resulted in the increase of phospho-Akt (Ser473) and phospho-mTOR (Ser2448), then activation of mTOR targets, p70S6K and 4E-BP1. Conclusions: The up-regulation of miR-122 may play an important role in the progress of renal cancer through activating PI3K/Akt signal pathway and could be a potential molecular target for anti-cancer therapeutics.

• Molecules and Cells
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• v.38 no.7
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• pp.643-650
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• 2015

The use of conditioned medium from mesenchymal stem cells may be a feasible approach for regeneration of bone defects through secretion of various components of mesenchymal stem cells such as cytokines, chemokines, and growth factors. Mesenchymal stem cells secrete and accumulate multiple factors in conditioned medium under specific physiological conditions. In this study, we investigated whether the conditioned medium collected under hypoxic condition could effectively influence bone regeneration through enhanced migration and adhesion of endogenous mesenchymal stem cells. Cell migration and adhesion abilities were increased through overexpression of intercellular adhesion molecule-1 in hypoxic conditioned medium treated group. Intercellular adhesion molecule-1 was upregulated by microRNA-221 in mesenchymal stem cells because microRNAs are key regulators of various biological functions via gene expression. To investigate the effects in vivo, evaluation of bone regeneration by computed tomography and histological assays revealed that osteogenesis was enhanced in the hypoxic conditioned medium group relative to the other groups. These results suggest that behavioral changes of endogenous mesenchymal stem cells through microRNA-221 targeted-intercellular adhesion molecule-1 expression under hypoxic conditions may be a potential treatment for patients with bone defects.

• Progress in Superconductivity and Cryogenics
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• v.25 no.3
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• pp.18-21
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• 2023

Pollution of the environment by micro-plastics is now a worldwide problem. Plastics are difficult to decompose and put a great load on the marine environment. Especially a plastic with a size of 5 mm or less is defined as micro-plastic and are carried by ocean currents over long distances, causing global pollution. These are not easily decomposed in the natural environment. In this paper, we aimed to experimentally demonstrate that micro-plastics in seawater can be continuously separated by electromagnetic Archimedes force. Using polyethylene particles of 3 mm in diameter as the separation target, a flow channel was fabricated and separation conditions were investigated by particle trajectory calculations for separation experiments. Based on the calculation results, a solenoid-type superconducting magnet was used as a source of magnetic field to conduct separation experiments of micro-plastics in seawater. Although a high separation rate was assumed in the simulation results, the experimental results did not show any significant improvement in the separation rate due to the electromagnetic Archimedes force. It was found that the gas generated by the electrolytic reaction may have inhibited the migration of the particles.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2839-2843
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• 2013

Objective: To investigate the effects of miR-106b on malignant characteristics of gastric cancer cells, and explore possible mechanisms. Methods: Expression of miR-106b, p21 and E2F was determined by real-time PCR. Transfection with miR-106b mimics was conducted, and gastric cancer cells with miR-106b overexpression were obtained. Cells transfected with mimic mutants and those without transfection served as negative and blank controls, respectively. Flow cytometry and transwell assays were adopted to detect the effects of miR-106b overexpression on cell cycle, migration and invasion of gastric cancer cells. Results:. The expression of miR- 106b in gastric cancer cells was significantly higher than that in normal gastric mucosa cells. Furthermore, the expression level of miR-106b rose according to the degree of malignacy among the three GC cell strains (MKN- 45 > SGC-7901 > MKN-28). Overexpression of miR-106b shortened the G0/G1 phase and accelerated cell cycle progression, while reducing p21 and E2F5, without any significant effects on the capacity for migration and invasion of gastric cancer cells. Conclusions: miR-106b may promote cell cycling of gastric cancer cells through regulation of p21 and E2F5 target gene expression.

• Journal of the Korean Geographical Society
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• v.43 no.6
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• pp.894-913
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• 2008

This study examines scale issues in the contemporary feminist migration literature. Scale appears as important, yet poorly understood concept in this field of study. The increasing attention to the feminization of migration requires not only gendered, but also scalar-sensitive approaches. Feminists criticize the conventional approach to the migration as a gender-blind approach that privileges national scale around which migration processes are organized. Claiming multiscalar and interscalar analyses, they propose investigations ranging from macro to micro processes which include globalized gendered division of labor, transnational family networks, and reproduction which takes place in and through the bodies and homes of migrant women. The migrant women, the major actors in recent transnational migration, cross various borders: the national boundaries and the public and private divides, in particular. This crossover can unsettle patriarchal gender relations which have been established based on the physical and symbolic division of nation-states and public/private spheres. Blurring these divisions accompanies social construction of various scales. The transnational family networks of migrant women, for example, show the construction of a transnational scale by migrant women as well as globalization from below. This paper points out misunderstandings of scale in the feminist migration literature and attempts to fill the gaps by introducing the meanings and implications of scales developed mostly by feminist geographers. In so doing, it promotes the interdisciplinary communication.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.5 no.2
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• pp.24-30
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• 1999

The migration mechanism of trace elements, Pb, Cd, Cu, Zn and Sn from brass food-contact utensiles was investigated. The migration of metals from brass was affected by the migration temperature (30, 60, 80 and $95^{\circ}C$), the simulant pH (2.5, 4.3, 6.0 and 7.0) and the migration time (30, 90, 180 and 360 min.). The amount of Pb migration was maximum at $95^{\circ}C$ with pH 2.5. This study indicated that the equilibrium of Pb migration was reached at 180 minutes. The mechanism of Pb migration was investigated with Scanning Electron Microscope (SEM) and Electron Probe Micro Analyzer (EPMA). To correlate the relations between the migration and the content of Pb in brass samples, ten brass samples having known content of Pb (the quantities of Pb in brass ; 0, 0.02, 0.09, 0.1, 0.2, 0.5, 0.9, 1.4, 5.4 and 9.2% (w/w)) were tested in the following conditions, 4% acetic acid, $95^{\circ}C$ and 30 minutes. The result represented that content of Pb in brass samples must be below 0.2% (w/w) to satisfy the 1.0 ppm (as Pb) of Korea Food Code.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.9
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• pp.5533-5537
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• 2013

MicroRNA-10b (miR-10b) has been reported to play an important role in some types of cancer, but the effects and possible mechanisms of action of miR-10b in the metastasis of nasopharyngeal carcinoma cells (NPC) have not been explored. The aim of the present study was to investigate the function of miR-10b in nasopharyngeal carcinoma and to determine the molecular mechanisms underlying its action. The MTT assay was used to assess proliferation of CNE-2Z cells. Wound healing and transwell migration assays were applied to assess cell migration and invasion, while and expression of E-cadherin and MMP-9 were detected using Western blot analysis. Real-time PCR was employed to detect the expression of genes related to migration and invasion and the $2^{-{\Delta}{\Delta}Ct}$ method was used to calculate the degree of expression. MTT assay showed the expression of miR-10b to have no effect on the proliferation of NPC cell lines. The wound healing assay showed that miR-10b mimics promoted the mobility and invasion of NPC cell lines. Inhibitors of miR-10b reduced the ability of NPC cell lines to migrate and invade. In addition, the expression of genes related to migration and invasion, such as E-cadherin, vimentin, and MMP-9, were confirmed to be different in the CNE-2Z NPC cell line transfected with miR-10b mimics and with miR-10b inhibitors. In the present study, miR-10b was found to upregulate the expression of MMP-9 and knockdown of miR-10b was found to significantly downregulate the expression of E-cadherin. On the whole, these results showed that miR-10b plays an important role in the invasion and metastasis of NPC cells.

• Korean Journal of Materials Research
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• v.20 no.1
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• pp.19-24
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• 2010

The current study examined Ag migration from the Ag paste bump in the SABiT technology-applied PCB. A series of experiments were performed to measure the existence/non-existence of Ag in the insulating prepreg region. The average grain size of Ag paste was 30 nm according to X-ray diffraction (XRD) measurement. Conventional XRD showed limitations in finding a small amount of Ag in the prepreg region. The surface morphology and cross section view in the Cu line-Ag paste bump-Cu line structure were observed using a field emission scanning electron microscope (FE-SEM). The amount of Ag as a function of distance from the edge of Ag paste bump was obtained by FE-SEM with energy dispersive spectroscopy (EDS). We used an electron probe micro analyzer (EPMA) to improve the detecting resolution of Ag content and achieved the Ag distribution function as a function of the distance from the edge of the Ag paste bump. The same method with EPMA was applied for Cu filled via instead of Ag paste bump. We compared the distribution function of Ag and Cu, obtained from EPMA, and concluded that there was no considerable Ag migration effect for the SABiT technology-applied printed circuit board (PCB).

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.18 no.8
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• pp.2450-2463
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• 2024

Many legacy information systems are currently being clouded. This is due to the advantage of being able to respond flexibly to the changes in user needs and system environment while reducing the initial investment cost of IT infrastructure such as servers and storage. The infrastructure of the information system migrated to the cloud is being integrated through the API connections, while being subdivided by using MSA (Micro Service Architecture) internally. DBMS (Database Management System) is also becoming larger after cloud migration. Scale calculation in most layers of the application architecture can be measured and calculated from auto-scaling perspective, but the method of hardware scale calculation for DBMS has not been established as standardized methodology. If there is an error in hardware scale calculation of DBMS, problems such as poor performance of the information system or excessive auto-scaling may occur. In addition, evaluating hardware size is more crucial because it also affects the financial cost of the migration. CPU is the factor that has the greatest influence on hardware scale calculation of DBMS. Therefore, this paper aims to calculate the conversion factor for CPU scale calculation that will facilitate the cloud migration between heterogeneous DBMS. In order to do that, we utilize the concept and definition of hardware capacity planning and scale calculation in the on-premise information system. The methods to calculate the conversion factor using TPC-H tests are proposed and verified. In the future, further research and testing should be conducted on the size of the segmented CPU and more heterogeneous DBMS to demonstrate the effectiveness of the proposed test model.

• Proceedings of the KIEE Conference
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• 2003.10a
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• pp.286-289
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• 2003

We investigated the influence of the properties of substrate material on the separation efficiency in microchip electrophoresis. We fabricated the various microchips and studied separation efficiency in microchannels composed of a single material such as quartz, glass, polydimethylsiloxane (PDMS), and polymethylmetha crylate (PMMA), as well as hybrid micro channels composed of different materials. New fabrication process for glass chip was suggested and some treatment is added to improve fabrication process in other chip. Separation efficiency was compared by measuring migration times and bandwidths of EOF and analytes in each microchip. The efficiency is the function of migration time, which is affected by the electroosmotic flow (EOF), and bandwidth of an analyte. EOF is highly dependent upon the characteristics of a microchannel wall surface. Migration time was more reproducible in silica chips than that of PDMS chip and more band broadening was observed in the microchip composed of hybrid material due to non-uniformity of surface charge density at the walls of the channel.