• Molecules and Cells
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• v.43 no.10
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• pp.856-869
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• 2020

To elucidate the mechanism of action of HOXA11-AS in modulating the cisplatin resistance of nasopharyngeal carcinoma (NPC) cells. HOXA11-AS and miR-454-3p expression in NPC tissue and cisplatin-resistant NPC cells were measured via quantitative reverse transcriptase polymerase chain reaction. NPC parental cells (C666-1 and HNE1) and cisplatin-resistant cells (C666-1/DDP and HNE1/DDP) were transfected and divided into different groups, after which the MTT method was used to determine the inhibitory concentration 50 (IC₅₀) of cells treated with different concentrations of cisplatin. Additionally, a clone formation assay, flow cytometry and Western blotting were used to detect DDP-induced changes. Thereafter, xenograft mouse models were constructed to verify the in vitro results. Obviously elevated HOXA11-AS and reduced miR-454-3p were found in NPC tissue and cisplatin-resistant NPC cells. Compared to the control cells, cells in the si-HOXA11-AS group showed sharp decreases in cell viability and IC₅₀, and these results were reversed in the miR-454-3p inhibitor group. Furthermore, HOXA11-AS targeted miR-454-3p, which further targeted c-Met. In comparison with cells in the control group, HNE1/DDP and C666-1/DDP cells in the si-HOXA11-AS group demonstrated fewer colonies, with an increase in the apoptotic rate, while the expression levels of c-Met, p-Akt/Akt and p-mTOR/mTOR decreased. Moreover, the si-HOXA11-AS-induced enhancement in sensitivity to cisplatin was abolished by miR-454-3p inhibitor transfection. The in vivo experiment showed that DDP in combination with si-HOXA11-AS treatment could inhibit the growth of xenograft tumors. Silencing HOXA11-AS can inhibit the c-Met/AKT/mTOR pathway by specifically upregulating miR-454-3p, thus promoting cell apoptosis and enhancing the sensitivity of cisplatin-resistant NPC cells to cisplatin.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.4
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• pp.454-462
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• 2018

This study was conducted to examine SNS addiction proneness and interpersonal satisfaction among undergraduate students and the relationships between these two variables, as well as to establish baseline data for appropriate intervention of SNS addiction prevention. The participants of this study were 316 undergraduate students in D and K city, and data were collected between June 30 and July 30, 2017. Data were collected by a self-administered online survey and analyzed by descriptive statistics, t-tests, and Pearson's correlation coefficients using SPSS. The results showed that SNS addiction proneness and interpersonal satisfaction were negatively correlated (r=-0.57, p<0.01), indicating students with higher SNS addiction had lower interpersonal satisfaction. There were no significant differences in SNS addiction proneness and interpersonal satisfaction by gender (t=0.05, p=0.963), number of SNS networks (t=0.66, p=0.513), or number of SNS-only networks (t=-1.24, p=0.216). Students who used SNS for data collection showed significantly higher interpersonal satisfaction (t=3.02, p=0.030); however, there was no significant differences in SNS addiction proneness among purposes for using SNS (t=0.39, p=.759). The results of this study will be useful baseline data for developing an intervention to improve interpersonal satisfaction and prevent SNS addiction among undergraduate students.

• Journal of Korean society of Dental Hygiene
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• v.12 no.2
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• pp.345-353
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• 2012

Objectives : The author has studied about correlation of gingival exposure upon smiling and oral facial status that reduce facial aesthetic. Methods : The subjects in this study are 91 female vulunteers who were in aged $21.4{\pm}1.89$ in Suwon. Objectives should be normal oral and facial status without the prosthodontic, orthodontic appliance or conqenital missing tooth, and agree to be examined the oral status and impression taking. 1.Measure the length of gingival exposure upon smiling. 2.Measure of the size on central incisor. 3.Measure of Facial. SPSS(SPSS 10.0 for windows, SPSS Inc, Chicago, USA) was utilized for calculating the correlation coefficient between gingival exposure upon smiling and facial status. Regression analysis was calculated in order to predict the R square for gingival exposure upon smiling. Results : 1.Correlation coefficient between the gingival exposure and length of maxillary central incisor was calculated as reversed correlation(r=-.302, p<0.01), and between the gingival exposure and the ratio of the length of central incisor/width of central incisor was revealed as reversed correlation(r=-.250, p<0.05) on smiling. 2.There was correlation between the gingival exposure and the facial height(r=.351, p<0.01), the lower facial height(r=.454, p<0.01) and the upper lip height(r=.274, p<0.01) upon smiling. 3.There was correlation between the gingival exposure and the ratio of the facial height/facial width(r=.358, p<0.05), the ratio of the upper facial height/facial width(r=.214, p<0.05), and the ratio of the lower facial height/facial height(r=.383, p<0.01) upon smiling. 4.The equation of the regression analysis for gingival exposure upon smiling could be estimated as gingival exposure upon smiling=-5.139+.279${\times}$lower facial height-.615${\times}$maxillary central incisal length-.05${\times}$nasolabial angle. Conclusions : Considering these results, it recommended that treatment planning should be designed in consideration of such factors as the length of maxillary central incisor, facial height, upper lip height and lower facial height, in order to promote the easthetic problems of face on smiling.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.3
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• pp.59-65
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• 2009

To investigate a posttraumatic stress, social support and work burden and to identify high risk group and related factors which were exerted influence on posttraumatic stress of firefighters. Data were gathered from total 264 firefighters consisting of 85 fire distinguishers & rescue workers, 96 emergency medical personnel and 83 in ambulance & fire truck drivers in C province and were analyzed 22 items by IES-R, 20 work burden items by Choi(2000), 8 social support items by Oh(2006) using SPSSWIN 14.0 program. Posttraumatic stress of general characteristics by work was showed significant difference in age, marriage status, education, position, office duration, mobilization frequency and experience of traumatic events. Posttraumatic stress were showed significant difference in 3 groups and fire distinguishers & rescue workers is the highest group. Posttraumatic stress was correlated with work burden(r=.317, p<0.01) for fire distinguishers & rescue workers, social support(r=-.331, p<0.01) and work burden(r=.522 p<0.001) for emergency medical personnel and work burden(r=.454, p<0.01) for ambulance & fire truck drivers. The high risk groups are fire distinguishers & rescue workers(60%), emergency medical personnel(55.2%)and ambulance & fire truck drivers(45.8%). Related factors are the experience seeing victim's danger for fire distinguishers & rescue workers(odds ratio=1.216, 95% confidence interval:1.068-1.383), and are work burden(odds ratio=1.100, 95% confidence interval:1.043-1.159) and office duration(odds ratio=1.010, 95% confidence interval 1.001-1.018) for emergency medical personnel, and are the experience seeing victim's danger(odds ratio=1.178, 95% confidence interval:1.010-1.373), age(odds ratio=1.129, 95%confidence interval:1.020-1.249), work burden(odds ratio=1.103, 95% confidence interval:1.034-1.177) for ambulance & fire truck drivers.

• Journal of Ecology and Environment
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• v.40 no.1
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• pp.75-83
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• 2016

Background: In order to evaluate the effect of pH, known as a critical factor for shaping the biogeographical microbial patterns in the studies by others, on the bacterial diversity, we selected two sites in a similar geographical location (site 1; north latitude 35.3, longitude 127.8, site 2; north latitude 35.2, longitude 129.2) and compared their soil bacterial diversity between them. The mountain soil at site 1 (Jiri National Park) represented naturally acidic but almost pollution free (pH 5.2) and that at site 2 was neutral but exposed to the pollutants due to the suburban location of a big city (pH 7.7). Methods: Metagenomic DNAs from soil bacteria were extracted and amplified by PCR with 27F/518R primers and pyrosequenced using Roche 454 GS FLX Titanium. Results: Bacterial phyla retrieved from the soil at site 1 were more diverse than those at site 2, and their bacterial compositions were quite different: Almost half of the phyla at site 1 were Proteobacteria (49 %), and the remaining phyla were attributed to 10 other phyla. By contrast, in the soil at site 2, four main phyla (Actinobacteria, Bacteroidetes, Proteobacteria, and Cyanobacteria) composed 94 %; the remainder was attributed to two other phyla. Furthermore, when bacterial composition was examined on the order level, only two Burkholderiales and Rhizobiales were found at both sites. So depending on pH, the bacterial community in soil at site 1 differed from that at site 2, and although the acidic soil of site 1 represented a non-optimal pH for bacterial growth, the bacterial diversity, evenness, and richness at this site were higher than those found in the neutral pH soil at site 2. Conclusions: These results and the indices regarding diversity, richness, and evenness examined in this study indicate that pH alone might not play a main role for bacterial diversity in soil.