• 제목/요약/키워드: miR-361-3p

검색결과 9건 처리시간 0.019초

The Effect of miR-361-3p Targeting TRAF6 on Apoptosis of Multiple Myeloma Cells

  • Fan, Zhen;Wu, Zhiwei;Yang, Bo
    • Journal of Microbiology and Biotechnology
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    • 제31권2호
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    • pp.197-206
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    • 2021
  • microRNA-361-3p (miR-361-3p) is involved in the carcinogenesis of oral cancer and pancreatic catheter adenocarcinoma, and has anti-carcinogenic effects on non-small cell lung cancer (NSCLC). However, its effect on multiple myeloma (MM) is less reported. Here, we found that upregulating the expression of miR-361-3p inhibited MM cell viability and promoted MM apoptosis. We measured expressions of tumor necrosis factor receptor-associated factor 6 (TRAF6) and miR-361-3p in MM cells and detected the viability, colony formation rate, and apoptosis of MM cells. In addition, we measured expressions of apoptosis-related genes Bcl-2, Bax, and Cleaved caspase-3 (C caspase-3). The binding site between miR-361-3p and TRAF6 was predicted by TargetScan. Our results showed that miR-361-3p was low expressed in the plasma of MM patients and cell lines, while its overexpression inhibited viability and colony formation of MM cells and increased the cell apoptosis. Furthermore, TRAF6, which was predicted to be a target gene of miR-361-3p, was high-expressed in the plasma of patients and cell lines with MM. Rescue experiments demonstrated that the effect of TRAF6 on MM cells was opposite to that of miR-361-3p. Upregulation of miR-361-3p induced apoptosis and inhibited the proliferation of MM cells through targeting TRAF6, suggesting that miR-361-3p might be a potential target for MM therapy.

N6-Methyladenosine modification (m6A) of circRNA-ZNF638 contributes to the induced activation of SHF stem cells through miR-361-5p/Wnt5a axis in cashmere goats

  • Ronghuan Yin;Ronglan Yin;Man Bai;Yixing Fan;Zeying Wang;Yubo Zhu;Qi Zhang;Taiyu Hui;Jincheng Shen;Siyu Feng;Wenlin Bai
    • Animal Bioscience
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    • 제36권4호
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    • pp.555-569
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    • 2023
  • Objective: The objective of this study was to investigate the effects of N6-Methyladenosine modification-circRNA-zinc finger protein 638 (m6A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats. Methods: The m6A modification of ZNF638 was analyzed using methylation immunoprecipitation with real-time quantitative polymerase chain reaction technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m6A dependence were evaluated through the overexpression of circRNA-ZNF638/its m6A-deficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3'- untranslated region was analyzed through Dual-luciferase reporter assay. Results: The m6A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m6A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of Wnt5a gene in SHF-stem cells. We further demonstrated that the internal m6A modification within circRNA-ZNF638 is required for mediating the miR-361-5p/Wnt5a pathway to regulate the induced activation of SHF stem cells through an introducing of m6A-deficient mutant of circRNA-ZNF638. Conclusion: The circRNA-ZNF638 contributes the proper induced activation of SHF-stem cells in cashmere goats in m6A-dependent manner through miR-361-5p/Wnt5a axis.

간호대학생의 임상실습 만족도에 영향을 미치는 요인 (Factors Influencing Satisfaction on Clinical Practice in Nursing Students)

  • 전민경;김미숙
    • 한국산학기술학회논문지
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    • 제18권1호
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    • pp.40-48
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    • 2017
  • 본 연구는 간호대학생의 정서조절능력, 스트레스 대처방식과 임상실습 만족도 간의 관계를 파악하고, 간호대학생의 임상실습 만족도에 영향을 미치는 요인을 파악하기 위한 서술적 조사연구이다. 자료 수집은 K시와 B시에 소재한 4개의 간호대학에서 3, 4학년에 재학 중인 간호대학생 201명을 대상으로 2016년 5월 1일부터 2016년 6월 30일까지 자가보고 설문지를 통하여 조사하였다. 수집된 자료는 SPSS/Win v 20.0을 이용하여 t-test, ANOVA, Scheffe's test, Pearson's correlation coefficient, stepwise multiple regression으로 분석하였다. 간호대학생의 감정조절 능력 평균은 5점 만점에 $3.79{\pm}0.48$점, 적극적 대처는 4점 만점에 $2.79{\pm}0.41$점, 소극적 대처는 $2.53{\pm}0.36$점, 임상실습 만족도는 5점 만점에 $3.58{\pm}0.46$점 이었다. 임상실습 만족도는 정서조절능력(r=.391, p<.001), 적극적 대처(r=.361, p<.001) 및 소극적 대처(r=.276, p<.001)와 통계적으로 유의한 상관관계가 있는 것으로 나타났다. 또한 임상실습 만족도에 영향을 미치는 주요 요인으로는 전공만족도(${\beta}=-.318$, p<.001)와 정서조절 능력(${\beta}=.230$, p<.001)으로, 이들 모형의 설명력은 28.1%로 나타났다. 본 연구결과를 바탕으로 간호대학생의 임상실습 만족도를 향상시키기 위해서는 간호대학생의 전공만족도를 높이고, 긍정적인 정서조절 능력을 향상시킬 수 있는 효과적인 프로그램의 개발이 필요하리라 사료된다.

Factors Influencing Intra-Operative Body Temperature in Laparoscopic Colectomy Surgery under General Anesthesia: An Observational Cohort

  • Kong, Mi Jin;Yoon, Haesang
    • Journal of Korean Biological Nursing Science
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    • 제19권3호
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    • pp.123-130
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    • 2017
  • Purpose: This study aimed to identify factors influencing intra-operative core body temperature (CBT), and to develop a predictive model for intra-operative CBT in laparoscopic abdominal surgery. Methods: The prospective observational study involved 161 subjects, whose age, weight, and height were collected. The basal pre-operative CBT, pre-operative blood pressure, and heartbeat were measured. CBT was measured 1 hour and 2 hours after pneumoperitoneum. Results: Explanatory factors of intra-operative hypothermia (< $36^{\circ}C$) were weight (${\beta}=.361$, p< .001) and pre-operative CBT (${\beta}=.280$, p= .001) 1 hour after pneumoperitoneum (Adjusted $R^2=.198$, F= 7.56, p< .001). Weight was (${\beta}=.423$, p< .001) and pre-operative CBT was (${\beta}=.206$, p= .011) 2 hours after pneumoperitoneum (Adjusted $R^2=.177$, F= 5.93, p< .001). The researchers developed a predictive model for intra-operative CBT ($^{\circ}C$) by observing intra-operative CBT, body weight, and pre-operative CBT. The predictive model revealed that intra-operative CBT was positively correlated with body weight and pre-operative CBT. Conclusion: Influence of weight on intra-operative hypothermia increased over time from 1 hour to 2 hours after pneumoperitoneum, whereas influence of pre-operative CBT on intraoperative hypothermia decreased over time from 1 hour to 2 hours after pneumoperitoneum. The research recommends pre-warming for laparoscopic surgical patients to guard against intra-operative hypothermia.

간호대학생의 자아존중감, 간호전문직관, 인지적 정서조절 전략이 임상실습스트레스에 미치는 영향 (Effects of Self-esteem, Nursing Professionalism, Cognitive Emotion Control Strategy on Clinical Practice Stress in Nursing Students)

  • 강미란;정경숙
    • 디지털융복합연구
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    • 제18권6호
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    • pp.353-361
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    • 2020
  • 본 연구의 목적은 간호대학생의 자아존중감, 간호전문직관, 인지적 정서조절 전략이 임상실습스트레스에 미치는 영향을 확인하기 위함이다. 연구대상은 B시 소재 간호대학에 재학 중이며 임상실습을 2학기 이상 경험한 학생 160명을 대상으로 하였으며, 자료수집은 2019년 12월 1일부터 15일까지 시행하였다. 자료분석은 기술통계, t-test, ANOVA, Pearson's corelation coefficient, Scheffe 사후검증과 다중회귀분석을 이용하였다. 연구결과, 임상실습스트레스는 5점 만점에 평균 3.19점이었다. 임상실습스트레스는 자아존중감(r=.22, p=.005), 간호전문직관(r=-.26, p=.001)과는 음의 상관관계를, 인지적 정서조절전략(r=.38, p<.001)과는 양의 상관관계를 보였다. 임상실습스트레스의 영향요인은 부적응적 인지적 정서조절전략(β=.46, p<.001), 간호전문직관(β=-.20, p<.001), 학년(β=.20, p=.002), 간호학 선택 동기(β=-.15, p=.019)이었으며 설명력은 총 35.5%로 나타났다. 따라서 임상실습에 대한 스트레스 감소를 위하여 간호전문직관 향상과 함께 스트레스 상황 시 부적응적 인지적 정서조절 전략을 낮출 수 있는 프로그램을 개발하고 적용해 불 것을 제언한다.

구취발생에 관여하는 구강내부요인에 관한 연구 (A study on the intraoral factor related to oral malodor)

  • 정미애;이은숙
    • 한국치위생학회지
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    • 제8권1호
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    • pp.119-131
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    • 2008
  • This study was peformed in order to find out the relationship between the causing factors and the production of each gas 01 oral malodor, to contribute the oral malodor control at dental clinic as well as to establish the effective application of malodor control project for public oral health program 127 patients from 20 to 40 years old who had been visited for preventive dental cares were participated for the study. Such items as caries status, periodontal status, salivary flow, viscosity, pH. Snyder test, plaque deposit and tongue plaque were checked through the oral examination in order to find out the contributing factors Hydrogen sulfide, Methyl mercaptan, Di-methyl sulfide and Ammonia gas components were checked with Oral-Chroma and Attain, the oral malodor check units. Not only the corelation coefficiencies but also the multi-way variance analysis were calculated between each causing factor and each component of oral malodor gases to estimate the contributing factors of the oral malodor. 1. There was no relationship between the caries status and each component pf the oral malodor such as sulfur compound or Ammonia, both in laboratory test and VAS test (pF0.05). It revealed negative relationship between Hydrogen sulfide and FT(rM-0.1904. pE0.05) as well as the VAS and FT (rM-0.210. pE0.05). S0, it was estimated that the less oral malador was recognized when caries state changed to filled state in Hydrogen sulfide laboratory test or VAS test 2. High relationship was showed between salivary flow and Hydrogen sulfide (rM-0.183, pM0.039), Methyl mercaptan(rM-0.234, p-0.008). Dimethyl sulfide(rM-0.234, pM0.008) and Ammonia(-0.361. pM0.001) gas(pE0.05). 3. There was a high relationship between M-PHP(Modified-Patient Hygiene Performance Index) and tong plaque all kinds of sulfide(rM0.249. pM0.005). Ammonia gas component(rM0.232, pM0.009). 4. It was found that considerable relationship was appeared between the periodontal status and Ammonia gas (rM0.274, pM0.002), so, it should be needed to control Ammonia. Such dental Cares as the prevention or early treatment of periodontal disease and the accelerating the salivary flow as well as reducing the amounts and activities of filament or spiral typed oral micro-organism were recommended for adults, not only for dental care program at the dental clinics but also for public health programs, in order to promote the oral health and quality of file for individual and community peoples.

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치위생과 학생의 감염관리에 대한 인지도와 실천도 조사 (A study on recognition and practice of dental hygiene students for Infection control dental hygiene major courses)

  • 이지영;정미경
    • 한국치위생학회지
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    • 제9권2호
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    • pp.73-88
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    • 2009
  • The purpose of the study was to provide the basic for the prevention of infection control by analyzing the level of recognition and practice of dental hygiene students. It is important for dental clinic staffs to know and practice infection control measures in favor of effective infection control. Thus, this study conducted an one-month questionnaire survey (during October 2008) for dental hygiene students who ever experienced in 16-week clinical practice, and analyzed total 206 questionnaire forms as collected from them, so that it could determine potential associations between their recognition and practice of infection control and thereby suggest an efficient solution for infection control in dental hygiene. 1. It was found that our students' recognition about infection control averaged $4.49{\pm}.58$ points on the whole, and they were best aware of instrumental disinfection and sterilization among other measures, which was followed by hand washing, extirpation control, post-sterilization management, personal protective outfit and instrumental surface maintenance respectively. 2. It was found that our students' practice of infection control averaged $3.85{\pm}.57$ points on the whole, and they practiced hand washing most actively among other measures, which was followed by extirpation control, post-sterilization management, instrumental disinfection and sterilization, personal protective outfit and instrumental surface maintenance respectively. 3. It was found that our students scored higher mean points in recognition about every measure of infection control than those in practice (t=15.676, p=0.000). Particularly, it was notable that there were significant differences between their mean points in recognition and practice of some infection control measures, such as instrumental surface maintenance (t=15.361, p=0.000), personal protective outfit (t=15.245, p=0.000) and instrumental disinfection and sterilization (t=11.169, p=0.000). But there was least significant difference between mean points in recognition and practice of hand washing (t=5.460, p=0.000). 4. For potential associations between recognition and practice of infection control, it was found that higher recognition was in significantly positive associations with higher practice in every measure of infection control (r=.478, p=.000), such as extirpation control (r=.630, p=.000), instrumental disinfection and sterilization (r=.477, p=.000) and post-sterilization management (r=.433, p=.000). 5. It was found that there were differences in our students' recognition depending upon availability of infection control guideline (t=4.587, p=.011), and there were significant differences in practice depending on necessity of infection control education on a statistical basis (t=2.229, p=.027). Overall, it is found that our dental hygiene students are very likely to practice hand washing and extirpation control, because both of these measures are relatively easy to practice or are considered legally binding.

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영세 노인들의 자기효능.사회적지지.우울간의 관계 분석 (The Relationship Among Self-Efficacy, Social Support and Depression of The Poor Elderly)

  • 송남호;김선미
    • 지역사회간호학회지
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    • 제11권1호
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    • pp.158-171
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    • 2000
  • The elderly population will increase from 5.8% in 1996 to 12.5% in 2020. The related problems of health will also become a very important issue in the future. Therefore. it is important to address the problems of geriatic nursing and geriatic health. The purpose of this study was to examine the relationship among self-efficacy, social support and depression of the poor elderly. The subjects of this study were 42 poor elderly in Tae Jeon city. The data were analyzed by the SPSSWIN computer program and include AONVA. Pearson Correlation, frequency analysis. The Cronbach Alpha of self-efficacy tools was .8297, social support tools was .9187 and depression tools was .8887. The data were collected through personal interviews using a questionnaire from September 27 to October 23. 1999. The conclusion of this study are summarized as follows: 1. There were mean scores for self-efficacy at $55.92{\pm}11.84$ for social support $86.00{\pm}18.43$ and for depression $60.31{\pm}15.61$. 2. There was a significant positive correlation between self-efficacy and social support(r= .361. p<.05) in the poor elderly. There was a significant negative correlation between social support and depression(r= -.640, p<,01), self-efficacy and depression(r= -.182) in the poor elderly. 3. In a significant test in the general characters of the subjects and in the higher social support level. we obtained the following results: living with partner is. have son is. living family members is. the higher economic level is. 4. In a significant test in the general characters of the subjects and in the lower depression level, we can obtained the following results: The old man is. living with partner is. living family members is. the higher economic level is, the higher health level is.

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The Iron Status of Very Low Birth Weight Infants Receiving Multiple Erythrocyte Transfusions during Hospitalization in the Neonatal Intensive Care Unit

  • Park, Sook-Hyun;Kim, Heng-Mi
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • 제18권2호
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    • pp.100-107
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    • 2015
  • Purpose: We investigated the iron status of very low birth weight infants receiving multiple erythrocyte transfusions during hospitalization in the neonatal intensive care unit (NICU). Methods: We enrolled 46 very low birth weight infants who were admitted to the Kyungpook National University Hospital between January 2012 and December 2013. Serum ferritin was measured on their first day of life and weekly thereafter. We collected individual data of the frequency and volume of erythrocyte transfusion and the amount of iron intake. Results: A total of 38 (82.6%) of very low birth weight infants received a mean volume of $99.3{\pm}93.5mL$ of erythrocyte transfusions in NICU. The minimum and maximum serum ferritin levels during hospitalization were $146.2{\pm}114.9ng/mL$ and $456.7{\pm}361.9ng/mL$, respectively. The total volume of erythrocyte transfusion was not correlated to maximum serum ferritin concentrations after controlling for the amount of iron intake (r=0.012, p=0.945). Non-transfused infants took significantly higher iron intake compared to infants receiving ${\geq}100mL/kg$ erythrocyte transfusion (p<0.001). Minimum and maximum serum ferritin levels of non-transfused infants were higher than those of infants receiving <100 mL/kg erythrocyte transfusions (p=0.026 and p=0.022, respectively). Infants with morbidity including bronchopulmonary dysplasia or retinopathy of prematurity received a significantly higher volume of erythrocyte transfusions compared to infants without morbidity (p<0.001). Conclusion: Very low birth weight infants undergoing multiply erythrocyte transfusions had excessive iron stores and non-transfused infants also might had a risk of iron overload during hospitalization in the NICU.