• 제목/요약/키워드: miR-29a

검색결과 228건 처리시간 0.032초

Identification of Genes Suitable for DNA Barcoding of Morphologically Indistinguishable Korean Halichondriidae Sponges

  • Park, Mi-Hyun;Sim, Chung-Ja;Baek, Jina;Min, Gi-Sik
    • Molecules and Cells
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    • 제23권2호
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    • pp.220-227
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    • 2007
  • The development of suitable genetic markers would be useful for defining species and delineating the species boundaries of morphologically indistinguishable sponges. In this study, genetic variation in the sequences of nuclear rDNA and the mitochondrial cytochrome c oxidase subunit 1 and 3 (CO1 and CO3) regions were compared in morphologically indistinguishable Korean Halichondriidae sponges in order to determine the most suitable species-specific molecular marker region. The maximal congeneric nucleotide divergences of Halichondriidae sponges in CO1 and CO3 are similar to those found among anthozoan cnidarians, but they are 2- to 8-fold lower than those found among genera of other triploblastic metazoans. Ribosomal internal transcribed spacer regions (ITS: ITS1 + ITS2) showed higher congeneric variation (17.28% in ITS1 and 10.29% in ITS2) than those of CO1 and CO3. Use of the guidelines for species thresholds suggested in the recent literature indicates that the mtDNA regions are not appropriate for use as species-specific DNA markers for the Halichondriidae sponges, whereas the rDNA ITS regions are suitable because ITS exhibits a low level of intraspecific variation and a relatively high level of interspecific variation. In addition, to test the reliability of the ITS regions for identifying Halichondriidae sponges by PCR, a species-specific multiplex PCR primer set was developed.

Genetic Diversity and Speciation of Rana rugosa (Amphibia; Ranidae)

  • Yang, Suh-Yung;Min, Mi-Sook;Kim, Jong-Bum;Suh, Jae-Hwa;Kang, Young-Jin
    • Animal cells and systems
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    • 제4권1호
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    • pp.23-30
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    • 2000
  • Horizontal starch gel electrophoresis for 29 populations (n=543) of the wrinkled frog, Rana rugosa, from Korea and Japan was peformed to assess the degree of genic variation and genetic diversity, and to understand the biogeographic pattern of distribution and speciation. A sum of 22 presumptive loci was screened from 17 enzymes and general proteins. Four loci, Aco, Est-3, Me-2, and Pgm, demonstrated high levels of polymorphism. The degree of average genetic variation of R. rugosa was P=22.7% (9.1-40.9%), Ho=0.086 (0.048-0.165) and He=0.090 (0.042-0.168). In the south-eastern region of the Korean peninsula (Chongsong, Yongchon, Ulsan, Kyongju, Pohang, yongdok and Ulchin), a few unique alleles in the Mpi locus were detected and their biogeographic implications were considered. The degree of genetic differentiation among the Korean populations was moderate (S=0.900), whereas the degree of genetic diversity between Korean and Japanese populations was notably high (S=0.687, D=0.293). This result corresponds with the data obtained by the mitochondrial cytochrome b gene sequence (Lee et al., 1999) suggesting that the Korean and Japanese R. rugosa might have evolved a specific level of genetic differentiation since their geographic isolation.

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Relationships among Bullying, Ego-Resilience, Perceived Stress, and College Adaptation in Nursing Students (간호대학생의 집단따돌림 피해 경험, 자아탄력성 및 지각된 스트레스와 대학생활 적응과의 관계)

  • Park, Mi Sook;Oh, Chung-Uk;Kang, Hye-Kyung
    • Journal of the Korea Convergence Society
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    • 제8권2호
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    • pp.73-81
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    • 2017
  • The purpose of this study was to investigate the relationship among bullying, ego-resilience, perceived stress and adaptation on college in nursing students. A total of 212 students from 1-4 grade in one nursing college were recruited into the study. The data were collected using questionnaire about bullying, ego-resilience, perceived stress and adaptation and were analyzed with PASW Statistics 18.0 program. Bullying and perceived stress had a significant negative association with nursing college adaptation(r=-.261, p<.001; r=-.261, p<.001) and nursing college adaptation was positively correlated with ego-resilience(r=.337, p<.001). The significant predictors of nursing college adaptation were grade(${\beta}$=-.216, p=.003), satisfaction with major(${\beta}$=-.245, p<.001), the latest semester grade(${\beta}$=.210, p=.028) and ego-resilience (${\beta}$=.324, p<.001), explaining 29.1% of the variance in nursing college adaptation(F=9.691, p<.001). According to the results, ego-resilience could be a key factor to improve college adaptation and bullying and perceived stress were associated with adaptation. Therefore many trials have to be need for enhancing college adaptation in a multiple and convergent way and it is suggested that the program to strengthen college adaptation should be developed.

Factors Relating Quality of Sleep: Comparison between Young-old People and Old-old People (수면의 질 영향 요인: 전기노인과 후기노인 비교)

  • Seo, Yeong-Mi;Kim, Jeong-Sook;Je, Nam Joo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • 제20권6호
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    • pp.332-341
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    • 2019
  • This study is a descriptive survey study to investigate the quality of sleep and to compare the factors relating quality of sleep in the young-old people and the old-old people. The subjects of the study were 200 elderly people who live in the G-region applying convenient expression method. Using a structured questionnaire, sociodemographic characteristics, lifestyle and health-related characteristics, depression, and quality of sleep were examined. We analyzed ${\chi}^2-test$, t-test, ANOVA(scheffe's test), Pearson's correlation coefficient and multiple regression using IBM SPSS WIN/21.0. The results of the study were as follows. The quality of sleep was significantly worse in the old-old people than in the young-old people. In young-old people, only depression (r=-.22, p=.038) showed a significant negative correlation with sleep quality. Old-old people was a significant negative correlation between sleep quality and depression (r=-.19, p=.045) and subjective health status (r=-.29, p=.002). In the young-old people, job, chronic illness, and economic level were significant influencing factors in the quality of sleep. In the old-old people, chronic disease, subjective health status, and smoking were significant influencing factors. The results of this study can be used as basic data for establishing tailored strategy for ages that can improve health of the elderly.

Variance of Agronomical Quantitative Traits in Mung Bean (Vigna radiata (L.) R. Wilczek var. radiata) Germplasm

  • Hyemyeong Yoon;Yu-Mi Choi;Kebede Taye Desta;Sukyeung Lee;Myong-Jae Shin;Xiaohan Wang;Joungyun Yi;Young-ah Jeon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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    • pp.31-31
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    • 2023
  • Mung bean(Vigna radiata (L.) R. Wilczek var. radiata) is a legume that originated in India. It is the third most cultivated legume in Korea after soybean and adzuki bean. Recently, the use of mung bean seeds and sprouts in trendy foods such as rice noodles and Chinese-style stir-fry is expanding thereby increasing its demand. Subsequently, improvement of mung bean varieties is also being actively conducted. In this study, the important agricultural characteristics of 324 mung bean germplasm were recorded and statistically investigated. Seeds of the mung bean germplasm were cultivated at an experimental field located in the National Agrobiodiversity Center (Jeonju, Korea) and 10 quantitative agricultural traits were investigated. Basic statistics, correlation analysis, and principal component analysis were then performed. The results showed significant variations of the quantitative traits among the germplasms (p < 0.05). The days to flowering, maturity, and growth were in the ranges of 31~80, 22~72, and 57~110 days with means of 45, 47, and 92 days, respectively. The highest frequency (f = 192) was for lodging score with 11~50%, while simultaneous maturity (f = 182) was below 50%. Other quantitative traits related to yield including the number of seeds per pod (CV = 10.9%), number of pods per plant (CV = 41.2%), and one-hundred seeds weight (CV = 36.6%) also showed significant variations. Correlation analysis showed positive correlations between the days to maturity and one-hundred seeds weight (r = 0.41) and the days to growth and simultaneous maturity (r = 0.39). In contrast, one-hundred seeds weight was negatively correlated to the number of pods per plant (r = -0.41) and the days to flowering (r = -0.29). Similarly, the days to growth and the number of pods per plant had a negative association with each other (r = -0.29). The principal component analysis revealed the number of days to maturity as the most influential variable along the first principal component (23.7%). In general, this study revealed wide variations in quantitative traits among the studied mung bean germplasm, which could provide several options for cultivar development.

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Knowledge, Confidence, and Learning Needs Regarding Advance Directives among Hospital Nurses (상급종합병원 간호사의 사전연명의료의향서에 대한 지식, 자신감 및 교육요구도)

  • Jang, Nan-Soon;Park, Hae-Sook;Kim, Mi-Ra;Lee, Joo-Yeon;Cho, Yeo-Won;Kim, Kyoung-Mi;Son, Youn-Jung
    • Journal of Korean Critical Care Nursing
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    • 제11권1호
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    • pp.35-45
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    • 2018
  • Purpose : Nurses' knowledge regarding advance directives may affect their administration of and confidence towards end of life care. This study aimed to describe the relationships of knowledge, confidence, and learning needs with advance directives among hospital nurses. Method : This cross-sectional study was performed at a tertiary university hospital in Seoul between September 25 and October 14, 2017. Convenience sampling was used to recruit nurses who provided bedside care and had at least 1 year of clinical experience. We used a validated self-report questionnaire. Results : The mean score of knowledge, confidence and learning needs were $5.00{\pm}1.73$, $29.81{\pm}7.52$, and $64.54{\pm}8.48$ respectively. Hospital nurses' knowledge, confidence and learning needs were significantly different according to age, job position, educational level and perceived advance directives. Knowledge regarding advance directives was significantly associated with confidence (r = .27, p < .001) and learning needs (r = .16, p = .005). Conclusion : Knowledge regarding advance directives was relatively low compared to the findings of previous studies. Therefore, nurses should be knowledgeable and encouraged to initiate advance directives. It is necessary to develop a standardized educational program regarding advance directives based on Korean cultures.

Relationship among Hardiness, Social Support and Burnout of Nurses Working in Emergency Department (응급실 간호사의 강인성, 사회적지지 및 소진간의 관계)

  • Park, Mi-Kyung
    • Journal of the Korea Convergence Society
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    • 제8권10호
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    • pp.397-405
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    • 2017
  • This study was to identify hardiness, social support, burnout which might influence burnout in emergency department nurses. Data were collected by self-reporting questionnaires from 115 nurses in 8 emergency department. Data analysis was done with SPSS/Win 21.0 program. Burnout showed significantly negative correlations with hardiness(r=-.390, p<.001), social support(r=-.400, p<.001) and hardiness showed significantly positive correlations with social support(r=.351, p<.001). As results, key factor was hardiness, social support, that continuing to work in emergency department, were significant predictors and accounted for 30.6%. Based on these results, burnout showed statistically significant differences depending on hardiness, social support and wanted department state. It is needed to develop a program in order to improve hardiness and social support of emergency department nurses.

Age and Growth of the Asian Clam Potamocorbula ustulata ustulata (Reeve, 1844) on the West Coast of Korea (한국 서해산 쇄방사늑조개, Potamocorbula ustulata ustulata (Reeve, 1844) 의 연령과 성장)

  • Ryu, Dong-Ki;Kim, Yeong-Hye
    • The Korean Journal of Malacology
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    • 제23권1호
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    • pp.17-23
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    • 2007
  • Samples of Potamocorbula ustulata ustulata were collected monthly from October 2004 to November 2005 in the Jujin estuary of Gochang, Chollabuk-do, west coast of Korean peninsula. Age of P. ustulata ustulata was determined by the rings on the shell. The relationship between the shell length and the ring diameter in each ring group was expressed as a regression line. Therefore, there is a correspondence in each ring formation. Based on the monthly variation of the marginal index (MI') of the shell, it is assumed that the ring of this species was formed once a year during October to December. The relationship between the shell length (SL) and the shell height (SH; mm) was highly correlated with shell height as the following equation: SH = 0.6438 SL + 0.5642 ($r^2\;=\;0.978$). The shell length (SL) - shell width (SW) relation was also expressed by the following equation: SW = 0.4352 SL - 0.5675 ($r^2\;=\;0.957$). Shell length (SL; mm) and the total weight (TW; g) followed: $TW\;=\;6.999\;{\times}\;10^{-5}\;SL^{3.2542}(r^2\;=\;0.975)$. Growth curves for the shell length and the total weight fitted to the von Bertalanffy's growth curve were expressed respectively as: $$SL_t=30.77[1-e^{-0.4572(t+0.7371)}],\;TW_t=4.87[1-e^{-0.4572(t+0.7371)}]^{3.2542}.$$

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Knowledge, Performance and Stress about Care for Delirium in Orthopedic Hospital Nurses (정형외과병원 간호사의 섬망간호에 대한 지식, 수행 및 스트레스)

  • Kim, Mi Young;Eun, Young
    • Journal of muscle and joint health
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    • 제20권1호
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    • pp.72-80
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    • 2013
  • Purpose: This study was conducted to investigate the correlation among nurses' knowledge, performance, and stress about care for delirium. Methods: The data were collected using the instruments for knowledge about nursing care (50 items), performance (23 items), and stress (20 items). Descriptive statistics and Pearson's correlation coefficient were used to analyze the data of 222 participants. Results: Nursing knowledge were different by clinical experience (F=3.12, p=.016), position at work (t=-2.54, p=.012), experience in caring patients with delirium (t=3.90, p<.001), and conflicts with other disciplines on matters related to delirium (t=4.00, p=.001). The difference in performance were associated with age (F=4.19, p=.001), clinical experience (F=2.67, p=.003), and whether there was a guideline for managing the patients with delirium (t=2.30, p=.022). Stress of nurses was different by whether they had a religion or not (t=-2.41, p=.017). The knowledge of care for delirium had the positive relationship with performance (r=.21, p=.001) and negative relationship with stress (r=-.29, p=.001). Conclusion: It is necessary to develop educational program and guideline for improving the knowledge and performance in care for delirium among nurses working at orthopedic hospitals.

The Association between Health Behaviors and Mental Health in Middle Aged

  • Choi Mi-Kyoung
    • Korean Journal of Health Education and Promotion
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    • 제21권4호
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    • pp.109-119
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    • 2004
  • This study was conducted to examine factors influencing mental health of middle-aged adults(N=203) in relation to sociodemographic variables, health behaviors, and social support. The survey with questionnaire was carried out for the subjects of the residents in two communities of a local city located in Gangwon-do, Korea from September 3 to 15, 2003. Data analysis procedure included stepwise multiple regression using mental health as the dependent variable, and sociodemographic variables, health behaviors, and social support as independent variables. There were significant differences in the mental health by birth place(t=-1.67, p<0.10), religion (t=2.27, p<0.05) and subjective economic status(F=2.29, p<0.10). Mental health showed significant positive correlations with both health behavior (r=0.462, p<0.001) and social support (r=0.142, p<0.05). Stepwise multiple regression analysis for mental health revealed that the most powerful predictor was health behavior(β=0.453, p<0.001). Health behavior and religion explained 24% of the variance. The results suggest the necessity of a intervention that considers the health behaviors should be included in middle-aged adults so as to promote mental health.