• Title/Summary/Keyword: miR-23a

Search Result 297, Processing Time 0.027 seconds

Muscle Activity Analysis of Lower Limb Training for Early Rehabilitation Cycling System in Supine Position (누운 자세에서 조기재활 자전거 시스템의 하지 운동 근 활성도 분석)

  • Shin, Sun Hye;Seo, Shin Bae;Yu, Mi;Jeong, Ho Chon;Cho, Kwang Soo;Hong, Jung Pyo;Hong, Chul Wn;Kwon, Tae Kyu
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.33 no.9
    • /
    • pp.753-760
    • /
    • 2016
  • This study was undertaken to develop a bed-type cycling system of lower limbs for rehabilitation. This system consists of two modes of cycling: active and passive. Different velocity and loads are provided for improving the muscle function recovery and increasing the muscular strength. To analyze the muscle activity pattern, we measured muscular activity of lower limbs in the rectus femoris (RF), biceps femoris (BF), tibialis anterior (TA), medial gastrocnemius (MG), and soleus (SOL), while cycling in the supine position, and based on the pedaling direction. A total of 18 young and 23 elderly, healthy subjects participated in this study. Muscle activity of MG muscles was significantly different in the two age groups. This study could provide the reference data to develop cycling exercises for lower limbs during rehabilitation of the elderly patients.

PCR Detection Method for Rapid Diagnosis of Bacterial Canker Caused by Clavibacter michiganensis on Tomato (토마토 궤양병 신속 진단을 위한 Clavibacter michiganensis의 PCR 검출법)

  • Park, Mi-Jeong;Back, Chang-Gi;Park, Jong-Han
    • Research in Plant Disease
    • /
    • v.24 no.4
    • /
    • pp.342-347
    • /
    • 2018
  • Bacterial canker caused by Clavibacter michiganensis is considered to be one of the most serious diseases, leading to economic damage to tomato worldwide. Diagnosis of the bacterial canker on tomato is known to be difficult because the causal pathogen is slow-growing on artificial media as well as causes latent infection in tomato. In this study, as a less time-consuming method, a specific primer set was newly designed for rapid detection of C. michiganensis. The method presented here is so simple, easy, and fast that it can be useful and practical in direct detection of the bacterial canker pathogen from tomato plants.

Composition and functional diversity of bacterial communities during swine carcass decomposition

  • Michelle Miguel;Seon-Ho Kim;Sang-Suk Lee;Yong-Il Cho
    • Animal Bioscience
    • /
    • v.36 no.9
    • /
    • pp.1453-1464
    • /
    • 2023
  • Objective: This study investigated the changes in bacterial communities within decomposing swine microcosms, comparing soil with or without intact microbial communities, and under aerobic and anaerobic conditions. Methods: The experimental microcosms consisted of four conditions: UA, unsterilized soil-aerobic condition; SA, sterilized soil-aerobic condition; UAn, unsterilized soil-anaerobic condition; and San, sterilized soil-anaerobic condition. The microcosms were prepared by mixing 112.5 g of soil and 37.5 g of ground carcass, which were then placed in sterile containers. The carcass-soil mixture was sampled at day 0, 5, 10, 30, and 60 of decomposition, and the bacterial communities that formed during carcass decomposition were assessed using Illumina MiSeq sequencing of the 16S rRNA gene. Results: A total of 1,687 amplicon sequence variants representing 22 phyla and 805 genera were identified in the microcosms. The Chao1 and Shannon diversity indices varied in between microcosms at each period (p<0.05). Metagenomic analysis showed variation in the taxa composition across the burial microcosms during decomposition, with Firmicutes being the dominant phylum, followed by Proteobacteria. At the genus level, Bacillus and Clostridium were the main genera within Firmicutes. Functional prediction revealed that the most abundant Kyoto encyclopedia of genes and genomes metabolic functions were carbohydrate and amino acid metabolisms. Conclusion: This study demonstrated a higher bacteria diversity in UA and UAn microcosms than in SA and SAn microcosms. In addition, the taxonomic composition of the microbial community also exhibited changes, highlighting the impact of soil sterilization and oxygen on carcass decomposition. Furthermore, this study provided insights into the microbial communities associated with decomposing swine carcasses in microcosm.

Comparison of Left Ventricular Volume and Function between 46 Channel Multi-detector Computed Tomography (MDCT) and Echocardiography (16 채널 Multi-detector 컴퓨터 단층촬영과 심초음파를 이용한 좌심실 용적과 기능의 비교)

  • Park, Chan-Beom;Cho, Min-Seob;Moon, Mi-Hyoung;Cho, Eun-Ju;Lee, Bae-Young;Kim, Chi-Kyung;Jin, Ung
    • Journal of Chest Surgery
    • /
    • v.40 no.1 s.270
    • /
    • pp.45-51
    • /
    • 2007
  • Background: Although echocardiography is usually used for quantitative assessment of left ventricular function, the recently developed 16-slice multidetector computed tomography (MDCT) is not only capable of evaluating the coronary arteries but also left ventricular function. Therefore, the objective of our study was to compare the values of left ventricular function quantified by MDCT to those by echocardiography for evaluation of its regards to clinical applications. Material and Method: From 49 patients who underwent MDCT in our hospital from November 1, 2003 to January 31, 2005, we enrolled 20 patients who underwent echocardiography during the same period for this study. Left ventricular end-diastolic volume index (LVEDVI), left ventricular end-systolic volume index (LVESVI), stroke volume index (SVI), left ventricular mass index (LVMI), and ejection fraction (EF) were analyzed. Result: Average LVEDVI ($80.86{\pm}34.69mL$ for MDCT vs $60.23{\pm}29.06mL$ for Echocardiography, p<0.01), average LVESVI ($37.96{\pm}24.52mL$ for MDCT vs $25.68{\pm}16.57mL$ for Echocardiography, p<0.01), average SVI ($42.90{\pm}15.86mL$ for MDCT vs $34.54{\pm}17.94mL$ for Echocardiography, p<0.01), average LVMI ($72.14{\pm}25.35mL$ for MDCT vs $130.35{\pm}53.10mL$ for Echocardiography, p<0.01), and average EF ($55.63{\pm}12.91mL$ for MOCT vs $59.95{\pm}12.75ml$ for Echocardiography, p<0.05) showed significant difference between both groups. Average LVEDVI, average LVESVI, and average SVI were higher in MDCT, and average LVMI and average EF were higher in echocardiogram. Comparing correlation for each parameters between both groups, LVEDVI $(r^2=0.74,\;p<0.0001)$, LVESVI $(r^2=0.69,\;p<0.0001)$ and SVI $(r^2=0.55,\;p<0.0001)$ showed high relevance, LVMI $(r^2=0.84,\;p<0.0001)$ showed very high relevance, and $EF (r^2=0.45,\;p=0.0002)$ showed relatively high relevance. Conclusion: Quantitative assessment of left ventricular volume and function using 16-slice MDCT showed high relevance compared with echocardiography, therefore may be a feasible assessment method. However, because the average of each parameters showed significant difference, the absolute values between both studies may not be appropriate for clinical applications. Furthermore, considering the future development of MDCT, we expect to be able to easily evaluate the assessment of coronary artery stenosis along with left ventricular function in coronary artery disease patients.

Overall Prevalence and Distribution of Knockdown Resistance (kdr) Mutations in Aedes aegypti from Mandalay Region, Myanmar

  • Naw, Haung;Su, Mya Nilar Chaw;Vo, Tuan Cuong;Le, Huong Giang;Kang, Jung-Mi;Jun, Hojong;Mya, Yi Yi;Myint, Moe Kyaw;Lee, Jinyoung;Sohn, Woon-Mok;Kim, Tong-Soo;Na, Byoung-Kuk
    • Parasites, Hosts and Diseases
    • /
    • v.58 no.6
    • /
    • pp.709-714
    • /
    • 2020
  • Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) of mosquitoes confer resistance to insecticides. Although insecticide resistance has been suspected to be widespread in the natural population of Aedes aegypti in Myanmar, only limited information is currently available. The overall prevalence and distribution of kdr mutations was analyzed in Ae. aegypti from Mandalay areas, Myanmar. Sequence analysis of the VGSC in Ae. aegypti from Myanmar revealed amino acid mutations at 13 and 11 positions in domains II and III of VGSC, respectively. High frequencies of S989P (68.6%), V1016G (73.5%), and F1534C (40.1%) were found in domains II and III. T1520I was also found, but the frequency was low (8.1%). The frequency of S989P/V1016G was high (55.0%), and the frequencies of V1016G/F1534C and S989P/V1016G/F1534C were also high at 30.1% and 23.5%, respectively. Novel mutations in domain II (L963Q, M976I, V977A, M994T, L995F, V996M/A, D998N, V999A, N1013D, and F1020S) and domain III (K1514R, Y1523H, V1529A, F1534L, F1537S, V1546A, F1551S, G1581D, and K1584R) were also identified. These results collectively suggest that high frequencies of kdr mutations were identified in Myanmar Ae. aegypti, indicating a high level of insecticide resistance.

Parameter Optimization and Automation of the FLEXPART Lagrangian Particle Dispersion Model for Atmospheric Back-trajectory Analysis (공기괴 역궤적 분석을 위한 FLEXPART Lagrangian Particle Dispersion 모델의 최적화 및 자동화)

  • Kim, Jooil;Park, Sunyoung;Park, Mi-Kyung;Li, Shanlan;Kim, Jae-Yeon;Jo, Chun Ok;Kim, Ji-Yoon;Kim, Kyung-Ryul
    • Atmosphere
    • /
    • v.23 no.1
    • /
    • pp.93-102
    • /
    • 2013
  • Atmospheric transport pathway of an air mass is an important constraint controlling the chemical properties of the air mass observed at a designated location. Such information could be utilized for understanding observed temporal variabilities in atmospheric concentrations of long-lived chemical compounds, of which sinks and/or sources are related particularly with natural and/or anthropogenic processes in the surface, and as well as for performing inversions to constrain the fluxes of such compounds. The Lagrangian particle dispersion model FLEXPART provides a useful tool for estimating detailed particle dispersion during atmospheric transport, a significant improvement over traditional "single-line" trajectory models that have been widely used. However, those without a modeling background seeking to create simple back-trajectory maps may find it challenging to optimize FLEXPART for their needs. In this study, we explain how to set up, operate, and optimize FLEXPART for back-trajectory analysis, and also provide automatization programs based on the open-source R language. Discussions include setting up an "AVAILABLE" file (directory of input meteorological fields stored on the computer), creating C-shell scripts for initiating FLEXPART runs and storing the output in directories designated by date, as wells as processing the FLEXPART output to create figures for a back-trajectory "footprint" (potential emission sensitivity within the boundary layer). Step by step instructions are explained for an example case of calculating back trajectories derived for Anmyeon-do, Korea for January 2011. One application is also demonstrated in interpreting observed variabilities in atmospheric $CO_2$ concentration at Anmyeon-do during this period. Back-trajectory modeling information introduced in this study should facilitate the creation and automation of most common back-trajectory calculation needs in atmospheric research.

Comparision of Self-disclosure and Self-esteem between Baccalaureate and Associate Degree of Nursing Students. (4년제와 3년제 간호대학생의 자기표출과 자아존중감의 비교)

  • Lee, Kyu-Eun;Ha, Na-Sun;Lee, Yong-Mi;Yom, Young-Hee;Yee, Jung-Ae
    • Journal of Korean Academy of Nursing Administration
    • /
    • v.6 no.2
    • /
    • pp.165-176
    • /
    • 2000
  • The purpose of this study was investigate the self-disclosure and self-esteem of senior nursing students in two types of nursing education programs : baccalaureate and associate programs. The subjects were comprised of 365 associate degree nursing students from five junior college and 297 baccalaureate degree nursing students from six universities. The data were collected conveniently by self reporting questionnaires given to the students from september 13 to october 23, 1999. The instruments used JSDQ and Self-esteem scale. The data were analyzed by $SAS/PC^+$ program using descriptive statistics, $x^2-test$, t-test, pearson correlation coefficient. The results of this study are as follows : 1. There was significant difference in religion(p=.001), motive of entrance of college( p=.001) and satisfaction of nursing practice(p=.038) between baccalaureate degree nursing students and associate degree. 2. The extent of self-disclosure in baccalaureate degree nursing students had significantly higher than that in associate degree nursing students(p=.0022). 3. An object for self-disclosure were girlfriend, mother, boyfriend and father successively. 4. The extent of self-esteem in baccalaureate degree nursing students had significantly higher than that in associate degree(p=.0224). 5. The relationship between self-disclosure and self-esteem revealed a positive significant correlation(r=.2628, p=.0001).

  • PDF

Fabrication of Compound K-loaded Polymeric Micelle System and its Characterization in vitro and Oral Absorption Enhancement in vivo

  • Hong, Sun-Mi;Jeon, Sang-Ok;Seo, Jo-Eun;Chun, Kyeung-Hwa;Oh, Dong-Ho;Choi, Young Wook;Lee, Do Ik;Jeong, Seong Hoon;Kang, Jae Seon;Lee, Sangkil
    • Bulletin of the Korean Chemical Society
    • /
    • v.35 no.11
    • /
    • pp.3188-3194
    • /
    • 2014
  • Compound K (CK) was formulated as polymeric micelles (PM) using Pluronic$^{(R)}$ F-127 to enhance the oral absorption of CK, an intestinal bacterial metabolite of ginseng protopanaxadiol saponin. The physicochemical properties of Ck-loaded PM were characterized and an in vitro transport study using the Caco-2 cell system as well as an in vivo pharmacokinetic study using SD rats was carried out. The hydrodynamic mean particle size of CK-loaded PM (CK-PM) was $254{\pm}23.45nm$ after rehydration and the drug loading efficiency was ca. 99.9%. The FT-IR spectroscopy, X-ray diffraction, differential scanning calorimetry and scanning electron microscopy data supported the presence of a new solid phase in the PM. The $P_{app}$ value of in vitro Caco-2 cell permeation of CK-PM and the oral absorption of CK was enhanced about 1.2-fold and 2.6-fold compared to CK suspension, respectively, showing that the present PM formulation enabled an enhancement of oral CK absorption.

A Study on Risk Factors of Musculoskeletal Disorders among Selected Female Hair Dressers (일부 여성 미용종사자들의 근골격계 증상의 위험요인)

  • Song, Mi-Ra;Han, Sung-Hyun
    • Journal of Korean Society of Occupational and Environmental Hygiene
    • /
    • v.15 no.3
    • /
    • pp.250-260
    • /
    • 2005
  • This study was designed to find the symptom prevalence rates of musculoskeletal disorders and the risk factors of musculoskeletal disorders among selected hair dressers. Methods; The survey were conducted from July 1 to 30, 2004 for 459 hair dressers working in Seoul and Gwangju using self-administration questionnaire. The risk factors on musculoskeletal disorders have analysed by multiple linear regression analysis. The results of this study are summarized as follows: The experience rate of musculoskeletal disorders symptom in the last one year was 56.4%. The experience rates of each body parts were 36.4% on the shoulders, 30.5% on the legs and the feet, 28.5% on the low back, 27.0% on the hand and the fingers, 23.3% on the neck and 17.0% on the arms and the elbows. The prevalence rate of musculoskeletal disorders symptom in the last one week was 40.5%, those of each body parts were 24.0% on the legs and the feet, 21.4% on the shoulders, 20.7% on the low back, 15.7% on the hand and the wrists, 15.0% on the neck, 9.4% on the arms. The risk factors on musculoskeletal disorders were working posture(${\beta}=0.32$), perceived stress(${\beta}=0.19$), working period(${\beta}=0.16$), standing working over 10 hour(${\beta}=0.16$), hard working(${\beta}=0.11$). The adjusted determinant coefficient($R^2$) of this regression model was 0.267. Based on the results, in order to prevent musculoskeletal disorders of hair dressers, working posture shall be improved and leisure opportunities to relieve stress, and health management program shall be provided.

A Study on the Relationship Between Perceived Family Support & Self Concept of Cesarean Section Mothers (가족의 지지와 제왕절개술 산모의 자아개념간의 관계연구)

  • Chang, Myung-Ja;Lee, Mi-La
    • 모자간호학회지
    • /
    • v.1
    • /
    • pp.23-33
    • /
    • 1991
  • Recently, Cesarean Section has been revealed as major events in maternity nursing, because it has influence on tile formatter of the self concept of cesarean section mothers as well as later coping mechanism and mother-child relationship including the mothering role. The study is conducted to clarify the relationships between the perceired family support and the self concept of cesarean section mothers, and to find out other Influencing factors on the formation of the self concept. Measuring instrument used in this study was Lee's scale to measure the self concept and the degree of perceived family support of the cesarean section mothers, The subject were 155 cesarean section mothers who were selected conveniently. Volunteers answered self reporting questionnaires which asked about their self concept. Data was analyzed as follows using SAS. The relationship between scores of the self concept and degree of the perceived family support was analyzed by Pearson's product moment correlation. In order to identify the influencing effects of demographic variables on the formation of self concept & F test were used. Result were summarized as follows : 1. Scores of self concept of cesarean section mothers were significantly correlated with scores of the perceived family support (r=0.3438, p<0.01) These results show that the family support is closely related to the self concept of cesarean mothers and has positive effects on their coping capacity. 2. Scores of self-concept of those who have lower ducation background and are multipara were significantly higher than those who have higher education background and are primipara(M=29.90$\pm$9.37, 27.69$\pm$3.89, t=2.006, df=105.8, p=0.0480: M=27.75$\pm$3.49. 29.46$\pm$8.68, t=-1.7183, 2. p=0.08).

  • PDF