• Title/Summary/Keyword: miR-23a

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Evaluation of Nursing Educational Objectives Achievement & Related Factors in Nurses Within Five Years After Graduation: Focusing on Critical Thinking Disposition and Clinical Competence (일개대학 간호학과 졸업 후 5년 이내 간호사의 교육목표 달성도와 영향요인 -비판적 사고 성향과 임상수행능력을 중심으로)

  • Han, Mi-Hyun;Jeong, Seung-Eun;Kim, Jee-Yoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.12
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    • pp.836-846
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    • 2018
  • The purpose of this study was to evaluate nursing educational objectives achievement, critical thinking disposition, and clinical competence and their relationships in nurses within five years after graduation. Subjects were 82 nurses who graduated one nursing college within five years and 68 managers in nursing unit of hospital. Questions were given to nurses. They reported their achievement of educational objectives of the nursing college, critical thinking disposition and clinical competence. Nursing unit managers received the same questions to evaluate nurses working at their unit. Data were collected from January to march 2014 and analyzed by descriptive statistics, t-test, ANOVA, Pearson correlation and multiple regression with SPSS/WIN 23.0 program. Mean scores for achievement of educational objectives, critical thinking disposition, and clinical competence were $3.60{\pm}0.60$, $3.46{\pm}0.28$, and $4.17{\pm}0.56$, respectively. Educational objectives achievement showed significant correlations with critical thinking disposition (r = 0.52, p <. 001) and clinical competence (r = 0.52, p < .001). Regression analysis showed that critical thinking disposition (${\beta}=.30$, p = .018) and clinical competence (${\beta}=.26$, p = .029) were significant factors for predicting educational objectives achievement. Manager's evaluation score for educational objectives achievement and clinical competence was higher than graduated nurses' report (p < .001). This could be used as a feed- back to reset educational objectives and upgrade the curriculum. These study results could be used to, establish strategies to improve educational objectives achievement.

Crystal Structures of Ni2$^{2+}$ - and Tl$^+$ - Exchanged Zeolite X, $Ni_{17}Tl_{58}Si_{100}Al_{92}O_{384} and Ni_{12}Tl_{68}Si_{100}Al_{92}O_{384}$

  • Song, Mi Gyeong;Yun, Bo Yeong;Kim, Yang
    • Bulletin of the Korean Chemical Society
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    • v.22 no.2
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    • pp.164-170
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    • 2001
  • The crystal structures of fully dehydrated Ni2+- and Tl+ -exchanged zeolite X (Ni17Tl58-X, and Ni12Tl68-X; X=Si100Al92O384) have been determined by single-crystal X-ray diffraction techniques in the cubic space group Fd3 at $21(1)^{\circ}C$ (a=24.380(4) $\AA$, 24.660(4) $\AA$, respectively). Their structures have been refined to the final error indices R1=0.037 and R2=0.043 with 485 reflections, and R1=0.039 and R2=0.040 with 306 reflections, respectively, for which I >36(I). In Ni17Tl58-X, 17 Ni2+ ions per unit cell were found at only two sites: 15 at site I at the center of the hexagonal prism (Ni-O=2.203(9) $\AA)$ and the remaining 2 at site II near single six-oxygen rings in the supercage (Ni-O=2.16(3) $\AA).$ Fifty-eight Tl+ ions were found at five crystallographic sites: 28 at site II (Tl-O=2.626(8) $\AA)$, 2 at site I' in the sodalite cavity near the hexagonal prism (Tl-O=2.85(1) $\AA)$, another 2 at site II' in the sodalite cavity (Tl-O=2.77(1) $\AA).$ The remaining 26 were found at two nonequivalent Ⅲ' sites with occupancies of 23 and 3. In Ni12Tl68-X, 12 Ni2+ ions per unit cell were found at two sites: 10 at site I (Ni-O=2.37(2) $\AA)$ and the remaining 2 at site II (Ni-O=2.13(2) $\AA).$ Sixty-eight Tl+ ions were found at five crystallographic sites: 28 at site II (Tl-O=2.63(1) $\AA)$, 12 at site I' (Tl-O=2.62(1) $\AA)$, 2 at site II' (Tl-O=3.01(2) $\AA)$, and the remaining 26 at two III' sites with occupancies of 23 and 3. It appears that Ni 2+ ions prefer to occupy site I and II, in that order. The large Tl+ ions occupy the remaining sites, I', II, II' and two different III' sites. In both crystals, only the Ni2+ ions at site II were reduced and migrated to the external surface of zeolite X when these crystals were treated with hydrogen gas.

The Mediating Effect of Nursing Professionalism in the Relationship between Ehtical Values and Ethical Decision-making Confidence among Nursing Students (간호대학생의 윤리적 가치관과 윤리적 의사결정 자신감의 관계에서 간호전문직관의 매개효과)

  • Mi-Jung Kim;Eun-Ha Na
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.5
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    • pp.127-134
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    • 2024
  • This study aims to provide foundational data for fostering desirable ethical values and enhancing confidence in ethical decision-making among nursing college students by identifying the mediating effect on nursing professionalism on the relationship between ethical values and ethical decision-making confidence. The subjects of the study were survey of 163 third-year nursing students from one universities. The SPSS/23.0 program was used to analyze the data with descriptive statistics; an independent t-test, analysis of variance ANOVA, Pearson's correlation coefficient, and mediating effect analysis were performed using Process Macro 4.0. Ethical decision-making confidence among nursing students was found to be correlated with ethical values (r=.613, p<.001) and nursing professionalism (r=.548, p<.001). Nursing professionalism mediated the relationship between ethical values and ethical decision-making confidence. Based on these findings, it is necessary to establish a continuous and systematic ethics education program from the beginning of admission to nursing school, and to develop and implement various teaching methods that allow nursing students to experience ethical decision-making directly and indirectly, aiming to enhance their confidence in ethical decision-making.

Rapid diagnosis of experimental listeriosis in mice by polymerase chain reaction (중합효소연쇄반응을 이용한 실험적 리스테리아 감염증의 신속진단)

  • Kang, Ho-jo;Lee, Seong-mi;Suk, Ju-myoung;Lee, Deog-kyu;Son, Won-geun
    • Korean Journal of Veterinary Research
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    • v.38 no.3
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    • pp.559-564
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    • 1998
  • The polymerase chain reaction(PCR) assay was used for rapid diagnosis from blood and organ samples experimentally infected with Listeria monocytogenes. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L monocytogenes. Procedure A was based on dilution of the blood sample followed by lysis of bacterial cell and direct analysis of the lysate with PCR. In artificially infected blood samples with L monocytogenes, it was possible to detect fewer than 40 cells per ml of blood. However, L monocytogenes was detected low rates on infected organs by the direct PCR. In procedure B, enrichment cultivation was used to increase numbers of bacteria before lysis and PCR. L monocytogenes was detected from 23 samples of 24 liver and spleen, respectively, and 18 samples of 24 blood were found to be positive by PCR on a subset of 72 organ samples, whereas L monocytogenes were detected on 63 organ samples in classical culture technique. It was required to analyze including enrichment steps were 6h and 18h on the procedure A and B, respectively.

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Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction

  • Kang, In Jeong;Kang, Mi-Hyung;Noh, Tae-Hwan;Shim, Hyeong Kwon;Shin, Dong Bum;Heu, Suggi
    • The Plant Pathology Journal
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    • v.32 no.6
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    • pp.575-579
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    • 2016
  • Burkholderia glumae (bacterial grain rot), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), and Acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for A. avenae subsp. avenae and B. glumae, and transposase A gene sequence for X. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for B. glumae, 490 bp for X. oryzae, and 290 bp for A. avenae subsp. avenae with the $63^{\circ}C$ as an optimum annealing temperature. Samples collected from naturally infected fields were detected with two bacteria, B. glumae and A. avenae subsp. avenae but X. oryzae pv. oryzae was not detected. This assay can be used to identify pathogens directly from infected seeds, and will be an effective tool for the identification of the three pathogens in rice plants.

Investigation of the body distribution of load pressure and virtual wear design of short pants harnesses in flying condition (플라잉 상태에서 바지형태의 하네스에 대한 하중압력 분포 측정 및 가상착의 적용)

  • Kwon, MiYeon
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.3
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    • pp.11-21
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    • 2021
  • Virtual reality is currently mainly used in games, but is starting to be applied as a variety of media fields, such as broadcasting and film. Virtual reality provides more fun than reality, and can provide new experiences in areas that cannot be experienced in reality due to the constraints of time, space, and environment. In particular, as the social non-contact arena has increased due to COVID-19, it is being applied to education, health, and medical industries. The contents are further expanding into design and military fields. Therefore, the purpose of this study was to observe the change in distribution of load and pressure felt by the body in the flying state while wearing a short pants harness, which are mainly used in the game and entertainment industry. In the experiment, the average pressure in the flying state was measured by attaching a pressure sensor to the back and front of a human mannequin. As a result, it was confirmed that the load concentrated on the waist in the flying state was 44 N, with a pressure of 1353 kPa. The pressure distribution was concentrated in front of the center of gravity, and was measured was at 98% by the pressure sensors, with an average pressure value of approximately 15 kPa, and a pressure value of approximately 12 kPa at the back, which was measured at 67% by the pressure sensor. The results of the load and pressure distribution measurement are presented as fundamental data to improve the wearability and comfort of harnesses in the future, and are compared to actual measured pressure values by analyzing the clothing pressure in flight through virtual wear of harnesses through the CLO 3D program.

Variance of Agronomical Quantitative Traits in Mung Bean (Vigna radiata (L.) R. Wilczek var. radiata) Germplasm

  • Hyemyeong Yoon;Yu-Mi Choi;Kebede Taye Desta;Sukyeung Lee;Myong-Jae Shin;Xiaohan Wang;Joungyun Yi;Young-ah Jeon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2023.04a
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    • pp.31-31
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    • 2023
  • Mung bean(Vigna radiata (L.) R. Wilczek var. radiata) is a legume that originated in India. It is the third most cultivated legume in Korea after soybean and adzuki bean. Recently, the use of mung bean seeds and sprouts in trendy foods such as rice noodles and Chinese-style stir-fry is expanding thereby increasing its demand. Subsequently, improvement of mung bean varieties is also being actively conducted. In this study, the important agricultural characteristics of 324 mung bean germplasm were recorded and statistically investigated. Seeds of the mung bean germplasm were cultivated at an experimental field located in the National Agrobiodiversity Center (Jeonju, Korea) and 10 quantitative agricultural traits were investigated. Basic statistics, correlation analysis, and principal component analysis were then performed. The results showed significant variations of the quantitative traits among the germplasms (p < 0.05). The days to flowering, maturity, and growth were in the ranges of 31~80, 22~72, and 57~110 days with means of 45, 47, and 92 days, respectively. The highest frequency (f = 192) was for lodging score with 11~50%, while simultaneous maturity (f = 182) was below 50%. Other quantitative traits related to yield including the number of seeds per pod (CV = 10.9%), number of pods per plant (CV = 41.2%), and one-hundred seeds weight (CV = 36.6%) also showed significant variations. Correlation analysis showed positive correlations between the days to maturity and one-hundred seeds weight (r = 0.41) and the days to growth and simultaneous maturity (r = 0.39). In contrast, one-hundred seeds weight was negatively correlated to the number of pods per plant (r = -0.41) and the days to flowering (r = -0.29). Similarly, the days to growth and the number of pods per plant had a negative association with each other (r = -0.29). The principal component analysis revealed the number of days to maturity as the most influential variable along the first principal component (23.7%). In general, this study revealed wide variations in quantitative traits among the studied mung bean germplasm, which could provide several options for cultivar development.

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Quantitative Analysis of Bioactive Compounds in the Fruits of Crataegus pinnatifida by High-Performance Liquid Chromatography

  • Bae, Yoon-Ho;Cuong, To Dao;Lee, Jae-Hyun;Woo, Mi-Hee;Choi, Jae-Sue;Min, Byung-Sun
    • Natural Product Sciences
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    • v.18 no.2
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    • pp.83-88
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    • 2012
  • In order to facilitate the quality control of the fruits of Crataegus pinnatifida, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the three bioactive compounds: chlorogenic acid (1), rutin (2), and hyperin (3), which were selected as the chemical markers of C. pinnatifida. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254nm. All three calibration curves showed good linearity ($R^2$ > 0.998). The recoveries of three marker compounds were in the range of 94.87~111.52 %. The contents of chlorogenic acid (1), rutin (2), and hyperin (3) of the fruits of C. pinnatifida collected from 23 district markets in Korea, Japan, and China were 0.16~0.65 mg/g, 0.07~1.24 mg/g, and 0.03~0.62 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of the fruits of C. pinnatifida.

Development and Validation of the Cancer-Specific Posttraumatic Growth Inventory (암환자 특이형 외상후 성장 측정도구 개발 및 타당화)

  • Jung, Young-Mi;Park, Jin-Hee
    • Journal of Korean Academy of Nursing
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    • v.47 no.3
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    • pp.319-331
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    • 2017
  • Purpose: The purpose of this study was to develop a scale to evaluate posttraumatic growth in patients with cancer and to examine the validity and reliability of the scale. Methods: A literature review, semi-structured patient interviews and an expert panel consultation produced a 27 preliminary item questionnaire. Participants were 150 cancer patients recruited to test the reliability and validity of the preliminary scale. Data were analyzed using item analysis, exploratory factor analysis, convergent validity and internal consistency. Results: Item reduction and exploratory factor analysis led to 23 items, grouped into five subscales which were labelled new possibilities (6 items), coping skills (5 items), preciousness of life (5 items), relating to others (4 items), and personal strength (3 items). Convergent validity was evaluated by total correlation with the Functional Assessment of Cancer Therapy-General (r=.45, p<.001). The final scale demonstrated satisfactory internal consistency (Cronbach's ${\alpha}$ =.94). Conclusion: Findings from this study indicate that the Cancer-Specific Posttraumatic Growth Inventory has validity and reliability and is considered to be appropriate for assessing posttraumatic growth in patients with cancer.

Characterization of microbiota diversity of engorged ticks collected from dogs in China

  • Wang, Seongjin;Hua, Xiuguo;Cui, Li
    • Journal of Veterinary Science
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    • v.22 no.3
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    • pp.37.1-37.14
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    • 2021
  • Background: Ticks are one of the most common external parasites in dogs, and are associated with the transmission of a number of major zoonoses, which result in serious harm to human health and even death. Also, the increasing number of pet dogs and pet owners in China has caused concern regarding human tick-borne illnesses. Accordingly, studies are needed to gain a complete understanding of the bacterial composition and diversity of the ticks that parasitize dogs. Objectives: To date, there have been relatively few reports on the analysis of the bacterial community structure and diversity in ticks that parasitize dogs. The objective of this study was to investigate the microbial composition and diversity of parasitic ticks of dogs, and assessed the effect of tick sex and geographical region on the bacterial composition in two tick genera collected from dogs in China. Methods: A total of 178 whole ticks were subjected to a 16S ribosomal RNA (rRNA) next generation sequencing analysis. The Illumina MiSeq platform targeting the V3-V4 region of the 16S rRNA gene was used to characterize the bacterial communities of the collected ticks. Sequence analysis and taxonomic assignment were performed using QIIME 2 and the GreenGene database, respectively. After clustering the sequences into taxonomic units, the sequences were quality-filtered and rarefied. Results: After pooling 24 tick samples, we identified a total of 2,081 operational taxonomic units, which were assigned to 23 phyla and 328 genera, revealing a diverse bacterial community profile. The high, moderate and low prevalent taxa include 46, 101, and 182 genera, respectively. Among them, dominant taxa include environmental bacterial genera, such as Psychrobacter and Burkholderia. Additionally, some known tick-associated endosymbionts were also detected, including Coxiella, Rickettsia, and Ricketssiella. Also, the potentially pathogenic genera Staphylococcus and Pseudomonas were detected in the tick pools. Moreover, our preliminary study found that the differences in microbial communities are more dependent on the sampling location than tick sex in the tick specimens collected from dogs. Conclusions: The findings of this study support the need for future research on the microbial population present in ticks collected from dogs in China.