Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Analysis of Bioactive Compounds in the Fruits of Crataegus pinnatifida by High-Performance Liquid Chromatography

  • Bae, Yoon-Ho (College of Pharmacy, Catholic University of Daegu) ;
  • Cuong, To Dao (College of Pharmacy, Catholic University of Daegu) ;
  • Lee, Jae-Hyun (College of Oriental Medicine, Dongguk University) ;
  • Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu) ;
  • Choi, Jae-Sue (Department of Food Science and Nutrition, Pukyoung National University) ;
  • Min, Byung-Sun (College of Pharmacy, Catholic University of Daegu)
  • Received : 2012.02.12
  • Accepted : 2012.05.03
  • Published : 2012.06.30
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Abstract

In order to facilitate the quality control of the fruits of Crataegus pinnatifida, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the three bioactive compounds: chlorogenic acid (1), rutin (2), and hyperin (3), which were selected as the chemical markers of C. pinnatifida. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254nm. All three calibration curves showed good linearity ($R^2$ > 0.998). The recoveries of three marker compounds were in the range of 94.87~111.52 %. The contents of chlorogenic acid (1), rutin (2), and hyperin (3) of the fruits of C. pinnatifida collected from 23 district markets in Korea, Japan, and China were 0.16~0.65 mg/g, 0.07~1.24 mg/g, and 0.03~0.62 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of the fruits of C. pinnatifida.

Keywords

References

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