• Journal of Microbiology and Biotechnology
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• v.21 no.5
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• pp.509-514
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• 2011

This study was conducted to investigate the phenotypic and genotypic characteristics of Korean isolates of Cronobacter spp. (Enterobacter sakazakii). A total of 43 Cronobacter spp., including 5 clinical isolates, 34 food isolates, 2 environmental isolates, and 2 reference strains (C. sakazakii ATCC 29004 and C. muytjensii ATCC51329) were used in this study. Korean isolates of Cronobacter spp. were divided into 11 biogroups according to their biochemical profiles and 3 genomic groups based on the analysis of their 16S rRNA gene sequences. Biogroups 1 and 2 contained the majority of isolates (n=26), most of which were contained in 16S rRNA cluster 1 (n=34). Korean isolates of Cronobacter spp. showed diverse biochemical profiles. Biogroup 1 contained C. sakazakii GIHE (Gyeonggido Research Institute of Health and Environment) 1 and 2, which were isolated from babies that exhibited symptoms of Cronobacter spp. infection such as gastroenteritis, sepsis, and meningitis. Our finding revealed that Biogroup 1, C. sakazakii, is more prevalent and may be a more pathogenic biogroup than other biogroups, but the pathogenic biogroup was not represented clearly among the 11 biogroups tested in this study. Thus, all biogroups of Cronobacter spp. were recognized as pathogenic bacteria, and the absence of Cronobacter spp. in infant foods should be constantly regulated to prevent food poisoning and infection caused by Cronobacter spp.

• Journal of Navigation and Port Research
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• v.39 no.1
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• pp.15-21
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• 2015

In high precision positioning systems based on GNSS, ambiguity resolution is an important procedure. Correct ambiguity leads to positioning results which have high precision between millimeters and centimeters. However, when the ambiguity is determined incorrectly, ensuring accuracy and precision of the positioning result is impossible. An ambiguity validation test is required to obtain correct ambiguity when ambiguity resolution is performed based on the ILS (Integer Least Squares), which shows the best performance in point of theory and experiment when compared with other methods such as IR (Integer Rounding) and IB (Integer Bootstrapping). Comparison between the candidates of the validation test is needed to judge ambiguity correctly, because ILS searches for candidates of integer ambiguity, unlike other methods which calculate only one integer ambiguity. We analyzed the experimental performance of ambiguity validation tests. R-ratio, F-ratio and W-ratio were adopted for analysis. The performance of validation tests was evaluated by classifying normal operation, detection, missed detection and false alarm. As a result, strengths and weaknesses of validation tests was showed to experimental. we concluded that validation tests must be selected according to environment.

• Korean Journal of Food Science and Technology
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• v.48 no.6
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• pp.536-541
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• 2016

The purpose of this study was to evaluate changes in the quality characteristics of mackerel fish fillet under freezing storage conditions. The changes in product quality were determined by measuring pH, volatile basic nitrogen (VBN); thiobarbituric acid (TBA), lightness ($L^*$), redness ($a^*$), yellowness ($b^*$) values, shearing force, and microbiological evaluation (Total Plate Count, TPC) during 21 days of storage at -20, -5, and $-1^{\circ}C$, and their shelf-lives were established. TPC as an effective quality indicator was used to estimate the shelf-life via linear regression analysis. The TPC increased steadily with increasing storage periods at every temperature (p<0.05) and showed a significant relationship with temperature at $-1^{\circ}C$ (r=0.949), $-5^{\circ}C$ (r=0.919), and $-20^{\circ}C$ (r=0.905). Therefore, based on TPC, the shelf life of the mackerel fillet was noted to be 32 days when stored at $-1^{\circ}C$, more than 51 days when stored at $-5^{\circ}C$, and about 2 months when stored at $-20^{\circ}C$.

• Journal of dental hygiene science
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• v.15 no.6
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• pp.825-835
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• 2015

The purpose of this study was to examine the relationships between depression, stress, self-esteem, oral health-related quality of life (OHIP-14) in middle-aged women. Data were as collected by self-reported questionnaires from 205 middle-age women. The instruments were the Center for Epidemiologic Studies Depression scale (CES-D), Brief Encounter Psychosocial Instrument (BEPSI), Self-Esteem Scale (SES). The data were analyzed using t-test, one-way ANOVA, Pearson correlation coefficients and multiple regression with IBM SPSS Statistics ver. 21.0. Pearson correlation coefficient analysis found that oral health impact profile (OHIP) was significantly associated with depression (r=-0.560, p<0.001), stress (r=-0.595, p<0.001), self-esteem (r=0.522, p<0.001). The OHIP was explained 39.4% by stress (${\beta}$=-0.362, p<0.001), self-esteem (${\beta}$=0.203, p=0.009) using multiple regression analysis. These results indicate that the intervention program of oral health promotion for community-middle- aged women is needed from now on.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.278-286
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• 2021

Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.8
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• pp.124-134
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• 2017

The purpose of this study was to investigate the MBTI personality type, emotional intelligence, and stress coping strategies of nursing students and to examine the influence of these factors on their level of college adjustment.The subjects were 267 nursing students who are located in Chonnam city. The survey was conducted from March 6th to March 31st, 2017. The level of college adjustment showed a close correlation with the emotional intelligence(r=.29, p<.001) and stress coping strategies(r=.27, p<.001), and the level of MBTI personality type was not statistically insignificant in the nursing students. It was found that the effect of the emotional intelligence and stress coping strategies on the college life adjustment was 17.5%(F=8.03, p<.001). According to the results of this study, it is necessary to develop a systematic management program to integrate the educational curriculum and extra-curricular activities(in order to enhance the) emotional intelligence and stress coping strategies in nursing students. In addition, the type of psychological function and psychological temperament of the MBTI personality type were found not to have influenced the level of college adaptation of the nursing students and this should be confirmedin a large scale sample.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.11
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• pp.321-331
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• 2017

This study was conducted to identify communication types of nurses, satisfaction with nursing services and intent to revisit the hospital by hospitalized patients, and to investigate factors influencing intent to revisit the hospital at a time of expansion of comprehensive nursing care service. The subjects consisted of 123 hospitalized patients in sixgeneral hospitals with more than 100 beds in Gyeongsangnam-do. Data were collected by a self-report questionnaire from July 1 to July 30, 2017. The collected data were analyzed by t-test, ANOVA, Pearson's correlation coefficients, and hierarchical regression. There were significant differences in intend to revisit the hospital depending on age(F=9.83, p<0.001), gender(t=-3.82, p<0.001), marital status(t=2.24, p=0.028), education(F=11.92, p<0.001) and days of hospitalization(F=7.81, p<0.001). Intent to revisit the hospital indicated a significantly positive correlation between informative(r=0.609, p<0.001) and affiliative(r=0.536, p<0.001) communication types and satisfaction with nursing services(r=7.97, p<0.001), as well as a significantly negative correlation with authoritative communication types(r=-0.356, p<0.001). Factors influencing intent to revisit the hospital were informational communication type(${\beta}=0.38$, p=0.003) and satisfaction with nursing services(${\beta}=0.84$, p<0.001). Based on these findings, it is necessary to develop various educational programs and conduct systematic education to facilitate effective communication between nurses and patients in order to improve the quality of nursing service and satisfaction of patients admitted to comprehensive nursing care services.

• Journal of Nutrition and Health
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• v.42 no.5
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• pp.423-433
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• 2009

Folate and vitamin $B_{12}$ are essential cofactors for homocysteine (Hcy) metabolism. Homocysteinemia has been related with cardiovascular and neurodegenerative disease. We examined the effect of folate and/or vitamin $B_{12}$ deficiency on biomarkers of one carbon metabolism in blood, liver and brain, and analyzed the correlation between vitamin biomarkers in mild and moderate homocysteinemia. In this study, Sprague-Dawley male rats (5 groups, n = 10) were fed folatesufficient diet (FS), folate-deficient diet (FD) with 0 or 3 g homocystine (FSH and FDH), and folate-/vitamin $B_{12}$-deficient diet with 3 g homocystine (FDHCD) for 8 weeks. The FDH diet induced mild homocysteinemia (plasma Hcy 17.41 ${\pm}$ 1.94 nmol/mL) and the FDHCD diet induced moderate homocysteinemia (plasma Hcy 44.13 ${\pm}$ 2.65 nmol/mL), respectively. Although liver and brain folate levels were significantly lower compared with those values of rats fed FS or FSH (p < 0.001, p < 0.01 respectively), there were no significant differences in folate levels in liver and brain among the rats fed FD, FDH and FDHCD diet. However, rats fed FDHCD showed higher plasma folate levels (126.5 ${\pm}$ 9.6 nmol/L) compared with rats fed FD and FDH (21.1 ${\pm}$ 1.4 nmol/L, 22.0 ${\pm}$ 2.2 nmol/L)(p < 0.001), which is the feature of "ethyl-folate trap"by vitamin $B_{12}$ deficiency. Plasma Hcy was correlated with hepatic folate (r = -0.641, p < 0.01) but not with plasma folate or brain folate in this experimental condition. However, as we eliminated FDHCD group during correlation test, plasma Hcy was correlated with plasma folate (r = -0.581, p < 0.01), hepatic folate (r = -0.684, p < 0.01) and brain folate (r = -0.321, p < 0.05). Hepatic S-adenosylmethionine (SAM) level was lower in rats fed FD, FDH and FDHCD than in rats fed FS and FSH (p < 0.001, p < 0.001 respectively) and hepatic S-adenosylhomocysteine (SAH) level was significantly higher in those groups. The SAH level in brain was also significantly increased in rats fed FDHCD (p < 0.05). However, brain SAM level was not affected by folate and/or vitamin $B_{12}$ deficiency. This result suggests that dietary folate- and vitamin B12-deficiency may inhibit methylation in brain by increasing SAH rather than decreasing SAM level, which may be closely associated with impaired cognitive function in nutritional homocysteinemia.

• Journal of Ecology and Environment
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• v.35 no.1
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• pp.9-14
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• 2012

Yellow bitterns (Ixobrychus sinensis) are a small wetland bird common to Asian countries including South Korea, Japan, and China. The aim of this study is to describe diet of yellow bitterns during the breeding season in artificial wetland of northeastern South Korea between May to August 1999-2001. For the purposes of this paper, we observe the frequency of nest visiting by parents during the chick rearing period. A total of 98 boluses regurgitated by 52 chicks aged 1 day to 11 days after hatching form the sample and are shown to contain 323 food items. A bolus contained mean 3.8 items and weighs 0.2 g to 7.7 g. The most regularly occurring food items recorded are fish (63%) and insets (33%). In terms of fish, top mouth minnows (Pseudorasbora parva) and crucian carps (Carassius auratus) are frequently observed. In terms of insects, there are mosquitoes (Diptera), instars of dragonfly (Libelluidae), damselflies (Coenagrinonidae) and water bugs (Diplonychus japonicus). Yellow bitterns were also shown to feed on bull frogs (Rana catesbeiana), shrimp (Palaemonidae), and spiders (Araneae). The size of fish in a bolus ranged from 15.56 mm to 93.73 mm (mean, 37.08 mm). The amount of food can be observed to increase with the age of chicks (r = 0.279, P = 0.025, N = 64) but parents did not provide larger fish as chicks grew. Parent birds visited nests more frequently when they have a larger brood ($F_{1,21}$ = 14.529, P = 0.001). Our results suggest that fish is the most important prey during the breeding season and that age of chicks is related to amount of diet in yellow bitterns.

• Journal of environmental and Sanitary engineering
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• v.19 no.3 s.53
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• pp.13-21
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• 2004

This study was focused on the evaluation of Nanjido landfill site by leachate analysis. Several parameters, for example pH, BOD, $COD_{Mn},\;COD_{Cr}$, SS, TP, $NH_3-N$, Color, were selected as major leachate quality parameters. $BOD/COD_{Cr}$. was used to estimate the biodegradable portion in organic matter. Samples were collected at the leachate storage wells and leachate treatment facility inflow in each quarter of 2003. The results were as follows : 1. Inflow quality of treatment plant in 2003 was analysed as $pH\;7.3\~8.0,\;BOD\;12.4\~30.0mg/L,\;COD_{Mn}\;101.2\~130.3mg/L,\;COD_{Cr}\;122.5\~238.0mg/L,\;SS\;16.1\~115.3mg/L$, $T-P\;0.27\~0.80mg/L,\;NH_3-N\;208.0\~~354.0mg/L$, Color $110.3\~129.0$ unit. 2. $BOD/COD_{Cr}$ of inflow ranged between $0.07\~0.16$ indicating that the landfill had the properties of old-fill and organic portion in leachate was mostly persistent organic matter. 3. Though concentrations of BOD, COD, $NH_3-N$, Color in leachate storage wells were mostly higher in first landfill than in second landfill, $BOD/COD_{Cr}$ ranging from 0.03 to 0.20, showed reversely. 4. Correlation coefficient$(R^2)$ between color and other parameters related to organic matter showed mostly above 0.7 and especially highest value with $COD_{Mn}$ of 0.7985.