• Title/Summary/Keyword: miR-21

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Fabrication of LiNiO2 using NiSO4 Recovered from NCM (Li[Ni,Co,Mn]O2) Secondary Battery Scraps and Its Electrochemical Properties (NCM(Li[Ni,Co,Mn]O2)계 폐 리튬이차전지로부터 NiSO4의 회수와 이를 이용한 LiNiO2 제조 및 전기화학적 특성)

  • Kwag, Yong-Gyu;Kim, Mi-So;Kim, Yoo-Young;Choi, Im-Sic;Park, Dong-Kyu;Ahn, In-Sup;Cho, Kwon-Koo
    • Journal of Powder Materials
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    • v.21 no.4
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    • pp.286-293
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    • 2014
  • The electrochemical properties of cells assembled with the $LiNiO_2$ (LNO) recycled from cathode materials of waste lithium secondary batteries ($Li[Ni,Co,Mn]O_2$), were evaluated in this study. The leaching, neutralization and solvent extraction process were applied to produce high-purity $NiSO_4$ solution from waste lithium secondary batteries. High-purity NiO powder was then fabricated by the heat-treatment and mixing of the $NiSO_4$ solution and $H_2C_2O_4$. Finally, $LiNiO_2$ as a cathode material for lithium ion secondary batteries was synthesized by heat treatment and mixing of the NiO and $Li_2CO_3$ powders. We assembled the cells using the $LiNiO_2$ powders and evaluated the electrochemical properties. Subsequently, we evaluated the recycling possibility of the cathode materials for waste lithium secondary battery using the processes applied in this work.

The Effect of GCSB-5 a New Herbal Medicine on Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation

  • Cho, Hee Kyung;Kim, So-Yeon;Choi, Mi Jung;Baek, Seung Ok;Kwak, Sang Gyu;Ahn, Sang Ho
    • Journal of Korean Neurosurgical Society
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    • v.59 no.2
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    • pp.98-105
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    • 2016
  • Objective : Lumbar disc herniation can induce sciatica by mechanical compression and/or chemical irritation. The aim of this study was to compare the effects of GCSB-5 (Shinbaro$^{(R)}$) and NSAIDs on pain-related behavior and on the expressions of microglia, astrocytes, CGRP, TRPV1, IL-6, and CX3CL1 in a rat model of lumbar disc herniation. Methods : 112 male Sprague-Dawley rats underwent implantation of nucleus pulposus to a dorsal root ganglion (DRG). Rats were divided into five groups as follows; a saline group (the vehicle control group) (n=27), a 10 mg/kg aceclofenac group (the aceclofenac group) (n=22), and 100, 300 or 600 mg/kg GCSB-5 groups (the GCSB-5 100, 300, or 600 groups) (n=21 for each group). Rats were tested for mechanical allodynia at 3 days after surgery and at 1 day, 3 days, 7 days, 14 days, 21 days, 28 days, 35 days, 42 days, 49 days, and 56 days after treatment commencement. Immunohistochemical staining of microglia (Iba1), astrocytes (GFAP), CGRP, and TRPV1, and PCR for IL-6 and CX3CL1 were performed on spinal dorsal horns and DRGs at 56 days after medication commencement. Results : After 56 days of GCSB-5 300 administration, mechanical withdrawal thresholds were significantly increased (p<0.05), and immunohistochemical expressions of Iba1, GFAP, CGRP, and TRPV1 were reduced than other groups, but this difference was not statistically significant. Conclusion : These results indicate GCSB-5 reduces mechanical allodynia and downregulates neuroglial activity and the expressions of CGRP and TRPV1 in the spinal segments of a rat model of lumbar disc herniation.

The Biomechanical Analysis of Two and Half Rotation Technic of Penche in Rhythmic Gymnastics (리듬체조 퐁쉐 2회전 1/2턴 기술의 역학적 분석)

  • Seo, Se-Mi;Ryu, Ji-Seon;Kim, Tae-Sam
    • Korean Journal of Applied Biomechanics
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    • v.21 no.3
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    • pp.269-279
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    • 2011
  • This study was analyzed the characteristics on the stability of posture while conducting a through two and half rotation technic of pench$\acute{e}$ in rhythmic gymnastics. Two rhythmical gymnastics player(LKH and SSJ) who is a member of the national team were selected, and for obtain the kinematic and kinetic variables were used a ProReflex MCU 240 infrared camera(Qualisys, Sweden) and a Type9286A force platform(Kistler, Switzerland). The mechanical factors were computed by using Visual3D program and Matlab R2009a. During the landing and rotation phase the results showed following characteristics; 1) In medial-lateral and horizontal displacement of the support foot, LKH showed smaller movement than SSJ, but SSJ showed smaller movement than LKH in swing foot. LKH showed bigger movement in medial-lateral axis of COP and vertical axis of COG, but SSJ showed bigger movement in horizontal axis of COP and medial-lateral axis of COG. 2) SSJ showed bigger maximum horizontal and vertical velocity at P1 and P2 than LKH. 3) In the inclination angle of COP and COG, SSJ showed smaller change than LKH, but within medial-lateral tilt of the shoulder, LKH performed rotation motion in horizontal position than SSJ. There was no differences in each force components during rotation, but on landing phase, the results showed a characteristic that SSJ exerted bigger breaking force and vertical force than LKH.

Antioxidant and Cytotoxic Activity of Compounds from the Stem of Juncus effusus (등심초로부터 분리된 화합물의 항산화 활성 및 암세포 성장 억제 효과)

  • Kim, Ye Jin;Park, Chan Ik;Park, Jae Sung;Ahn, Eun Mi
    • Journal of Applied Biological Chemistry
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    • v.57 no.1
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    • pp.47-51
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    • 2014
  • The stems of Juncus effusus were extracted with 70% aqueous ethanol and the concentrated extract was partitioned with ethyl acetate, n-butanol and $H_2O$, successively. Two compounds were isolated from the ethyl acetate fraction through the repeated silica gel and Sephadex LH-20 column chromatographies. According to the results of physico-chemical and spectroscopic data including NMR and IR, the chemical structures of the compounds were determined as dehydroeffusol (1) and effusol (2). Dehydroeffusol and effusol exhibited potent scavenging activity for DPPH and ABTS radicals with the $IC_{50}$ values as $130{\pm}3.21$ and $79{\pm}1.53{\mu}M$ in DPPH assay, and as $39{\pm}3.51$ and $24{\pm}2.73{\mu}M$ in ABTS assay, respectively. The compounds also significantly inhibited the proliferation of human cancer cell lines, AGS and A549.

Outcomes of Abdominal Total Gastrectomy for Type II and III Gastroesophageal Junction Tumors: Single Center's Experience in Korea

  • Kim, Kyoung-Tai;Jeong, Oh;Jung, Mi-Ran;Ryu, Seong-Yeop;Park, Young-Kyu
    • Journal of Gastric Cancer
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    • v.12 no.1
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    • pp.36-42
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    • 2012
  • Purpose: The aim of this study was to evaluate the surgical outcomes of abdominal total gastrectomy, without mediastinal lymph node dissection for type II and III gastroesophageal junction (GEJ) cancers. Materials and Methods: We retrospectively reviewed surgical outcomes in 67 consecutive patients with type II and III GEJ cancers that were treated by the surgical resection between 2004 and 2008. Results: Thirty (45%) patients had type II and 37 (55%) had type III tumor. Among the 65 (97%) patients with curative surgery, 21 (31%) patients underwent the extended total gastrectomy with trans-hiatal distal esophageal resection, and in 44 (66%) patients, abdominal total gastrectomy alone was done. Palliative gastrectomy was performed in two patients due to the accompanying peritoneal metastasis. The postoperative morbidity and mortality rates were 21.4% and 1.5%, respectively. After a median follow up of 36 months, the overall 3-years was 68%, without any differences between the Siewert types or the operative approaches (transhiatal approach vs. abdominal approach alone). On the univariate analysis, the T stage, N stage and R0 resection were found to be associated with the survival, and multivariate analysis revealed that the N stage was a poor independent prognostic factor for survival. Conclusions: Type II and III GEJ cancers may successfully be treated with the abdominal total gastrectomy, without mediastinal lymph node dissection in the Korean population.

A Study on Health Knowledge, Health Promoting Behavior and Needs for Healthcare Service in the Elderly in a Rural Area (일 농촌지역 노인의 건강지식과 건강증진행위 및 의료 서비스 욕구에 관한 연구)

  • Park, Mi Hui;Choi, Eun Hee;Choi, Kyung Sook
    • Journal of East-West Nursing Research
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    • v.21 no.1
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    • pp.18-27
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    • 2015
  • Purpose: The purpose of this study was to investigate the relationship between health knowledge and health promoting behavior in the elderly. Methods: The participants of this study were 114 men and women over 65 years at P-myeon, Yeongju-si, Gyeongsangbuk-do. Data were collected from March 1st to April 9th in 2011. The survey was carried out via the face to face interview using structured questionnaires. Results: Eighty nine percent of the participants responded that they were aware of the types of healthcare service. The most desirable service was 'long term care insurance system'. The average of health knowledge was 7.94 (${\pm}1.51$) out of 10 and average of health promoting behavior was 2.81 (${\pm}0.30$) out of 4. Health promoting behavior showed a positive correlation with health knowledge (r=.189, p=.044). Conclusion: The health promoting behavior in the elderly was related with health knowledge. Therefore, the enhancement of health knowledge is needed to improve health promoting behavior in the elderly. Education for self care and providing information for health care are needed for health maintenance and improvement in elderly. In addition, program development for providing health knowledge and nursing intervention for supporting health promoting behavior are in need.

Screening and Characterization of Lactic Acid Bacteria Strains with Anti-inflammatory Activities through in vitro and Caenorhabditis elegans Model Testing

  • Lee, Hye Kyoung;Choi, Sun-Hae;Lee, Cho Rong;Lee, Sun Hee;Park, Mi Ri;Kim, Younghoon;Lee, Myung-Ki;Kim, Geun-Bae
    • Food Science of Animal Resources
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    • v.35 no.1
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    • pp.91-100
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    • 2015
  • The present study was conducted to screen candidate probiotic strains for anti-inflammatory activity. Initially, a nitric oxide (NO) assay was used to test selected candidate probiotic strains for anti-inflammatory activity in cultures of the murine macrophage cell line, RAW 264.7. Then, the in vitro probiotic properties of the strains, including bile tolerance, acid resistance, and growth in skim milk media, were investigated. We also performed an in vitro hydrophobicity test and an intestinal adhesion assay using Caenorhabditis elegans as a surrogate in vivo model. From our screening, we obtained 4 probiotic candidate lactic acid bacteria (LAB) strains based on their anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell cultures and the results of the in vitro and in vivo probiotic property assessments. Molecular characterization using 16S rDNA sequencing analysis identified the 4 LAB strains as Lactobacillus plantarum. The selected L. plantarum strains (CAU1054, CAU1055, CAU1064, and CAU1106) were found to possess desirable in vitro and in vivo probiotic properties, and these strains are good candidates for further investigations in animal models and human clinical studies to elucidate the mechanisms underlying their anti-inflammatory activities.

Application of Chlorophyll Fluorescence Imaging Technique to Estimate Fresh Weight in Kiwifruit (엽록소 형광이미징 기술을 이용한 키위과일의 생체중 예측)

  • Lee, Mi Kyung;Yoo, Sung Yung;Kim, Tae Wan;Ku, Hyun-Hwoi
    • Korean Journal of Environmental Agriculture
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    • v.39 no.2
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    • pp.138-141
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    • 2020
  • BACKGROUND: Fresh weight is one of the major quality measurement factors in determining the quality of fresh fruits. A practical method has been developed for rapid and non-destructive measurement using the Chlorophyll Fluorescence Image (CFI) technique to estimate changes in fresh weight of post-harvest products. METHODS AND RESULTS: Kiwifruit (Actinidia deliciosa) was used and measured for the fresh weight and CFI under different temperature conditions at 0, 10, and 20℃, from 0 to 21 days after storage (DAS). We observed the fresh weight of kiwifruit and measured the surface image for determining Fv/Fm value in terms of maximum quantum yield on each day. To estimate freshness of kiwifruit we applied linear regression between the measured fruit weights and Fv/Fm values. Results showed that fruit weights were reduced by 4% at 0℃, 6% at 10℃, and 14% at 20℃ for 21 days, respectively. And also, the value of Fv/Fm was shown as decreasing trend at all temperature conditions, especially at 20 ℃. Fv/Fm values showed highly significant correlation (R2>0.9) with fresh weight of kiwifruit at all different storage temperatures. CONCLUSION: Thus, CFI technique can be useful to estimate the fresh weight of kiwifruit.

Experience and Perception of Sexual Harassment During the Clinical Practice and Self-esteem among Nursing Students (간호학생의 임상실습 시 성희롱 피해경험, 성희롱 심각성 인식 및 자아존중감)

  • Kim, Tae Im;Kwon, Yun Jung;Kim, Mi Jong
    • Women's Health Nursing
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    • v.23 no.1
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    • pp.21-32
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    • 2017
  • Purpose: The purpose of this study was to investigate the experience and perception of sexual harassment during the clinical practice among nursing students and to identify the relationship between these variables and self-esteem. Methods: Participants included 191 senior nursing students who voluntarily participated. Sexual harassment experiences and perceptions were measured using the tools developed by the researcher. Self-esteem was measured using self-esteem inventory developed by Rogenberg. Results: 50.8% of the participants experienced sexual harassment. Physical harassment was the most common, followed by verbal, visual and gender-role related sexual harassment. 'Blocking the way' and 'Brushing one's body on purpose' were the most common behaviors. The perception of sexual harassment severity was 3.35 out of 5 points. There were negative correlation between sexual harassment experience and self-esteem (r=-.17, p=.013). The subjects who experienced sexual harassment showed lower self-esteem score than those who did not (t=-2.10, p=.037). The main offender was a male patient in 40s, and the psychiatric ward was the most vulnerable to sexual harassment. Conclusion: Results showed that sexual harassment in clinical practice among nursing students were serious. It is necessary to develop an educational program to prepare them for sexual harassment in clinical practice.

The Severity and Variables Influencing Depression in Cancer Patients with Pain (통증이 있는 암환자의 우울 정도 및 우울에 영향을 미치는 요인)

  • Kim, Hyun-Sook;Yun, Young-Ho;Lee, So-Woo;Heo, Dae-Seog;Son, Haeng-Mi;Huh, Bong-Yul
    • Journal of Hospice and Palliative Care
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    • v.2 no.2
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    • pp.125-137
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    • 1999
  • Purpose : Surveying the rates, severity, and variables influencing depression and their correlation between pain and depression in Korean cancer patients, we attempted to provide a basic database for the effective depression management program. Methods : The results of survey were colleted from 10 patients who were hospitalized at Seoul National University Hospital for cancer treatment from February to June of 1999. Factors of depression and the level of pain were examined by self-reported survey employing Korean version of Beck Depression Inventory(BDI) and an abridged version of Brief pain Inventory respectively. The purpose of this study and guidelines for the questionnaires were clearly explained to participating patients by Resgitered Nurses before answering the survey. Demographic and clinical characteristics of patients were compiled by reviewing their medical records in corporation with a family physician. The difference in the level of depression among patient groups was analyzed with the t-test and ANOVA, and the correlation between variables with Pearson correlation coefficient. Results : 1) 142 subjects comprised 79 male and 63 female, and their mean age was 51.86. 2) The mean scores of the worst pain for last 24-hours was 6.08(SD 2.23), the average pain for last 24-hours 4.44(SD 1.85), and the mean scores of pain at the time of survey 3.48(SD 2.25), while the mean scores of the least pain for last 24-hours 2.25(SD 1.83). 3) The mean BDI scores were 23.73(SD 0.99), and 55.6% of patients were evaluated to be in depression(cutting point 21). Scores of depression for cancer patients were higher than normal population. 4) The correlation between worst pain for last 24-hours and depression(r=0.252, P=0.002), average pain for last 24-hours and depression(r=0.225, P=0.007), present pain and depression(r=0.291, P=0.000) were significant. 5) Significant differences were found among groups of cancer patients with pain with respect to gender(t=3.59, p=0.000), level of education(F=4.063, P=0.009), ECOG(F=3.352, P=0.021). There was significant positive correlation between depression and pain(r=0.171, P=0.042). Conclusions : More than 50% of cancer patients with pain are suffering from depression. We have shown that the variables like the degree of pain, gender, level of education, ECOG, and age are significantly related to the depression in cancer patients. The findings of this study may be used for assessing high-risk patients in need of intervention and for planning effective therapeutic strategies for them after the routine assessment. Further study is necessary to investigate the cultural differences and the variables influencing on depression in Korean cancer patients.

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