• Natural Product Sciences
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• v.18 no.2
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• pp.97-101
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• 2012

Six lignan compounds, 1-(17,21-dihydroxyphenyl)-9-(12,13-dihydroxyphenyl)-1-nonanone (malabaricone C) (1), 7'-(3',4'-methylenedioxyphenyl)-8,8'-dimethyl-7-(3,4-dihydroxyphenyl)-butane (2), 7'-(3',4'-dimethoxyphenyl)-8,8'-dimethyl-7-(3-methoxy-4-hydroxyphenyl)-butane (3), 7-(4-hydroxy-3-methoxyphenyl)-7'-(3',4'-methylenedioxyphenyl)-8,8'-lignan-7-methyl ether (4), (+)-erythro-(7S,8R)-${\Delta}^{8^'}$-7-hydroxy-3,4,3',5'-tetramethoxy-8-O-4'-neolignan (5), and (+)-erythro-(7S,8R)-${\Delta}^{8^'}$-7-acetoxy-3,4,3',5'-tetramethoxy-8-O-4'-neolignan (6), were isolated from the seeds of Myristica fragrans. The chemical structures of these compounds were determined on the basis of spectroscopic analyses including 2D NMR. Compounds 1 - 6 were evaluated for their cytotoxic activity against the HL-60, MCF-7, and A549 cancer cell lines in in vitro.

• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.92-101
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• 2006

The bacterial diversity of the bovine rumen was examined using a PCR-based approach. 16S rDNA sequences were amplified and cloned from three fractions of rumen (solid, fluid, and epithelium) that are likely to represent different bacterial niches. A total of 113 clones were sequenced, and similarities to known l6S rDNA sequences were examined. About $47.8\%$ of the sequences had $90-97\%$ similarity to 16S rDNA database sequences. Furthermore, about $62.2\%$ of the sequences were $98-100\%$ similar to 16S rDNA database sequences. For the remaining $6.1\%$, the similarity was less than $90\%$. Phylogenetic analysis was also used to infer the makeup of the bacterial communities in the different rumen fractions. The Cytophaga-Flexibacter-Bacteroides group (CFB, $67.5\%$), low G+C Gram-positive bacteria (LGCGPB, $30\%$), and Proteobacteria $(2.5\%)$ were represented in the rumen fluid clone set; LGCGPB $(75.7\%)$, CFB$(10.8\%)$, Proteobacteria $(5.4\%)$, high G+C Gram-positive bacteria (HGCGPB, $5.4\%$), and Spirochaetes $(2.7\%)$ were represented in the rumen solid clone set; and the CFB group $(94.4\%)$ and LGCGPB $(5.6\%)$ were represented in the rumen epithelium clone set. These findings suggest that the rumen fluid, solid, and epithelium support different microbial populations that may play specific roles in rumen function.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.16 no.2
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• pp.116-122
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• 2013

Purpose: Preterm infants on parenteral nutrition are at a relatively high risk for hypertriglyceridemia because they have immature lipoprotein lipase activity. The purpose of this study was to analyze the clinical factors affecting lipid metabolism in preterm infants receiving parenteral nutrition and to evaluate the influence of intravenous heparin on serum triglycerides to determine the adequate heparin dose to prevent hypertriglyceridemia in preterm infants. Methods: A single-center retrospective review was conducted among preterm infants receiving parenteral nutrition between January 2006 and February 2011. In 75 patients, 110 determinations were performed within 28 days postnatal age. Demographic and clinical data, including laboratory parameters, the dose and the duration of lipid administration, and the amount of intravenous heparin, were analyzed. Results: Serum triglycerides were higher in the small for gestational age (SGA) infants than in the appropriate for gestational age infants ($185.5{\pm}134.9$ mg/dL vs. $126.9{\pm}101.9$ mg/dL, p=0.019). Birth weight, gestational age, and body weight were negatively correlated with serum triglyceride level (r=-0.289, p=0.002; r=-0.208, p=0.029; r=-0.287, p=0.002, respectively). The serum triglyceride level was statistically lower in preterm infants receiving 1 U/mL of heparin than in those receiving 0.5 U/mL heparin or no heparin. Conclusion: Preterm infants receiving parenteral nutrition, particularly SGA and extremely low birth weight infants, tend to have hypertriglyceridemia. Thus, administration of 1 U/mL of heparin rather than 0.5 U/mL or none may be helpful to prevent hypertriglyceridemia in preterm infants.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.2
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• pp.101-108
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• 2016

We investigated changes in the physiochemical quality of sailfin sandfish sauces fermented with two kojis during long-term storage at room temperature. Four fish sauces, including a control, were prepared by salt-fermentation with soybean koji (S-koji) or rice koji (R-koji) after autolysis. During storage, for 12 months, the color and levels of amino acids, total volatile base nitrogen (T-VBN) and organic acids but not moisture, salt or total nitrogen levels or pH differed markedly according to fish sauce type. The total nitrogen level was highest in S-koji, but there was no difference in the rate of increase in amino acid levels among the four fish sauces during storage. The T-VBN, of autolysis and S-koji were significantly higher than those of the control and R-koji during the entire storage period (P<0.05). The initial organic acid level did not differ among the four fish sauces, whereas it was notably higher in R-koji than in the other sauces at the end of storage. Hunter L, a and b values decreased in all fish sauces with increasing storage time. In conclusion, soybean koji may enhance the levels of nitrogen compounds as well as T-VBN in fish sauce, while rice koji reduced the formation of excess T-VBN and increased organic acid levels during storage at room temperature.

• The Korean Journal of Rehabilitation Nursing
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• v.9 no.1
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• pp.34-41
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• 2006

Purpose: This study was to analyze the relationship the physical disability, cognitive disorder and body image. Method: The research was a descriptive relationship study. A sample is composed of 101 hospitalized stroke patients. Data were collected from November, 2005 to December, 2005. The survey instruments used in the study Sharon and Glen's physical disability scale, Lim's cognitive disorder scale and Osgood's body image scale. The collected data were analyzed frequency, percentage, mean, standard deviation, ANOVA, Duncan test, Pearsons' correlation coefficients. Result: The level of physical disability the score was 2.26, cognitive disorder 1.84 and body image 3.54. and they were relation to significant correlation. The body image showed significant negative correlation with physical disability, cognitive disorder. Physical disability showed significant positive correlation with cognitive disorder(r=.639, p=.000), and significant negative correlation with body image(r=-.420, p=.000). Cognitive disorder showed significant negative correlation with body image(r=-.620, p=.000). There were significant differences of body image by general characteristics as follows: age(p=.000), occupation(p=.004), education(p=.008), disease(p=.007). monthly income(p=.006), burden of medical expenses(p=.001), duration of stroke(p=.008). Conclusion: There was a significant correlation between physical disability, cognitive disorder and body image. there will be considered useful nursing intervention effect to physical disability, cognitive disorder and body image of stroke patients.

• Journal of Life Science
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• v.23 no.1
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• pp.24-30
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• 2013

The purpose of this study was to identify genetic polymorphisms of the mitochondrial cytochrome b (mtDNA cyt b) gene in Korean black (KB) cattle breed and to analyze the genetic relationship between the KB and other breeds. We determined the complete sequence of the mtDNA cyt b gene in 38 KB cattle. We also analyzed their genetic diversity, and phylogenetic analysis was performed by comparison with Korean cattle (KC, called Hanwoo) and breeds from China and Japan. A nucleotide substitution was detected in the KB cattle, and two haplotypes were defined. In the neighbor-joining (NJ) tree, the haplotypes of KB were located in Bos taurus lineage with those of KC, Japanese black (JB), Yanbian and Zaosheng breeds. However, the haplotypes of Chinese breeds, excluding Yanbian and Zaosheng, were separated into B. taurus and B. indicus lineages. In the NJ tree of breeds based on Dxy genetic distances, Chinese breeds mixed with B. taurus and B. indicus lineages were located between B. indicus and B. taurus lineages. KB was contained within B. taurus lineage and was determined to be genetically more closely related to two Chinese (Yanbian and Zaosheng) breeds than to KC and JB. The haplotype distribution and the results of the phylogenetic analysis suggest that KB and KC have genetic differences in their mtDNA cyt b gene sequences.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.17
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• pp.7749-7754
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• 2015

Background: Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection condition for cervical cancer cell lines. Materials and Methods: $Lipofectamine^{TM}2000$ as the carrier, miR-101 mimic was transfected into Hela cells and Siha cells. Using green fluorescent protein as reporter gene, to set different groups according to cell seeding density, the amount of miRNA, miRNA and the proportion of Liposomes, Whether to add serum into medium to study their impact on the liposomal transfection efficiency. Finally, MTT assay was used to analyze the relative minimal cell toxicity of liposome reagents. Results: The seeding density of Hela cell line and Siha are $1.5{\times}10^4$ (per well of 24 well plates), miRNA amount is 1ul of both, the ratio of miRNA and liposome is 1:0.5 of Hela cell line; 1:0.7 of Siha cell line respectively, after 24 hours we can get the highest transfection efficiency. Compared with serum medium, only Siha cells cultured with serum-free medium obtained higher transfection efficiency before transfection (P<0.01). MTT assay showed that according to the above conditions which has the lowest cytotoxicity. Conclusions: The method of Liposome to transfected is a suitable way and it can be an efficient reagent for miRNA delivery for Hela cells and Siha cells in vitro. It may serve as a reference for the further research or application.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.16 no.2
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• pp.144-152
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• 2009

Purpose: The purpose of this study was to predict the risk factors for vascular complications among patients with type 2 diabetes. Method: The data were collected from August to September, 2007 using clinical examination and questionnaires. Patients (N=101) were recruited from the endocrinology department of P University hospital in D city. Descriptive statistics, Pearson correlation coefficients and multiple linear regression were used to analyze the data. Results: The cardiovascular risk of patients with diabetes was significantly related to self care behavior, family history, and smoking status. The risk of peripheral vascular complications was not related to predictors included in the study. With multivariate analyses, significant predictors of cardiovascular risk for these patients were self care behavior, family history, and smoking status ($R^2=.40$, p<.0001). Conclusion: The findings of this study indicate that smoking cessation and improving self-care behavior are essential to reduce the risk of cardiovascular complications among patients with diabetes. To enhance self-care practices for the patients with diabetes, nursing interventions, such as telephone counseling, problem focused nursing counseling, and peer group activities should be considered.

• Korean Journal of Crystallography
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• v.2 no.2
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• pp.28-31
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• 1991

4-크로로-4'-메톡시-2-니트로디페닐아민, (C12H11N2O3CL, FW=278.70)의 단것세포 상수는 a=8,169(3), b=8.883(1), c=9.150(1) h, α =82. 98(1), β=104.80(2), y=101.43(2)", V=627.3 A3, F(000)=288.0, Dc=1.48g/cm3, u=3.06cm-1, 7=295" K, 공간군 Pi, 번호 2, 삼사 정계 이며, Z=2이다. λ (Mo-Ka)=0.7107A을 사용 하여 수집한 독립적인 회절 반점 1541개로 구조분 석한 최종 신뢰도 값은 각각 R=0.032, Rw=0.033 이며, S=0.46이다. 본 화합물은 암모니아의 두개 의 수소 대신에 4-크로로-페닐기와 4-메톡시-페닐기로 치환된 물질로써, 질소와 두 페닐기 사이의 각과 결합거리 들은 각각 125.42", 1.362 및 1.428 A 인바 수소와 함께 SP2_혼성결합을 하고있다. 질소를 포함한 두 면간 각은 63.29"이다. 분자간 어 떠 한 수소결합을 하고 지지 않다.

• Journal of Families and Better Life
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• v.29 no.2
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• pp.101-111
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• 2011

The purpose of study was to examine the associations between relationship with parents, personality traits and friendship quality among college students. A total of 373 college students (mean age=20.45, 53.6% girls) responded to questionnaires IPPA-R(Raja, McGee & Stanton, 1992) and IPIP(Goldberg, 1999). Pearson Correlation and regression analysis were used for data analysis 1) Both male and female students who have positive experiences with parents positive experiences with their friends. 2) For male college students, extroversion, openness to experience, agreeableness and conscientiousness were positively correlated with friendship quality. However, for female students, only extroversion and agreeableness were positively associated with friendship quality whereas neuroticism was negatively associated with friendship quality. 3) A good relationships with significantly interacted with conscientiousness of personality traits in predicting male students' friendship quality. For female college students, the moderating effect of extroversion was found in the link between relationship with mother and friendship quality.