• 한국식품영양학회지
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• 제30권6호
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• pp.1286-1291
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• 2017

In this study, Pyracantha angustifolia (Franch.) C. K. Schneid was extracted with 70% methanol at room temperature for 48 hrs and concentrated under reduced pressure to measure its total polyphenol contents; furthermore, the effect of electron donating ability was examined. Methylene chloride, ethyl acetate, and methanol were used to fractionate the extract to testify total polyphenol contents, electron donating abilities, the removal abilities of superoxide radical as well as hydrogen peroxide. The total polyphenol contents were $2007.30{\pm}109.28{\mu}g\;GAE/mL$ in 70% methanol extract, $273.39{\pm}10.19{\mu}g\;GAE/mL$ in methylene chloride fraction, $80.57{\pm}0.64{\mu}g\;GAE/mL$ in ethyl acetate fraction, and $1,160.87{\pm}44.71{\mu}g\;GAE/mL$ in methanol fraction. The total polyphenol contents showed significant differences (p<0.05) between the solvents. The electron donating ability was $79.07{\pm}7.31%$ for 70% methanol extract, $22.34{\pm}0.64%$ for methylene chloride fraction, $5.33{\pm}0.28%$ for ethyl acetate fraction, and $32.26{\pm}1.10%$ for methanol fraction. The electron donating abilities were significantly different(p<0.05) between the solvents. The removal ability of superoxide radical was $0.018{\pm}0.003$ for 70% methanol extract, $0.007{\pm}0.002$ for methylene chloride fraction, $0.0147{\pm}0.003$ for ethyl acetate fraction, and nothing for methanol fraction. The measurement of hydrogen peroxide decomposition was $0.022{\pm}0.0046$ for 70% methanol extract, $0.0027{\pm}0.0015$ for methylene chloride fraction, $0.0037{\pm}0.0012$ for ethyl acetate fraction, and $0.0009{\pm}0.0001$ for methanol fraction.

• 한국식품영양학회지
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• 제31권4호
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• pp.543-548
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• 2018

This study was conducted to investigated the antioxidant activities of extract for Megmoondong fruit. Liriope platyphylla L. was extracted with 70% methanol at room temperature for 48 hr and concentrated under reduced pressure to measure its total polyphenol contents and electron donating ability. The total polyphenol contents were $7,253.50{\pm}335.43{\mu}g\;GAE/mL$ in 70% methanol extract, $1,239.77{\pm}9.30{\mu}g\;GAE/mL$ in methylene chloride fraction, $919.30{\pm}50.83{\mu}g\;GAE/mL$ in methanol fraction, $105.44{\pm}2.04{\mu}g\;GAE/mL$ in ethyl acetate fraction. The total polyphenol contents showed significant differences (p<0.05) between the solvents. The electron donating ability was $69.17{\pm}12.61%$ for 70% methanol extract, $33.11{\pm}1.77%$ for methylene chloride fraction, $5.19{\pm}2.59%$ for ethyl acetate fraction, and $20.16{\pm}1.04%$ for methanol fraction. The electron donating abilities were significantly different (p<0.05) between the solvents. The removal ability of superoxide radical was $0.0174{\pm}0.0007$ for 70% methanol extract, $0.0164{\pm}0.0007$ for methylene chloride fraction, $0.0172{\pm}0.0007$ for ethyl acetate fraction, and nothing for methanol fraction. The measurement of hydrogen peroxide decomposition was $0.0985{\pm}0.1021$ for 70% methanol extract, $0.0896{\pm}0.0893$ for methylene chloride fraction, $0.0115{\pm}0.0085$ for ethyl acetate fraction, and $0.0170{\pm}0.0180$ for methanol fraction. The Liriope platyphylla L. extracts obtained from methylene chloride showed significantly relevant results in the total polyphenol contents and electron donating ability, which was higher than the original extract.

• 한국식품위생안전성학회지
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• 제1권1호
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• pp.13-21
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• 1986

Two short-term bioassays were employed to asses the mutagenic and clastogenic activities in browning reaction of pentose-creatine, pentose-glycine and pentose-creatine-glycine browning reaction model system. Methylene chloride extract of rhamnose-creatine-glycine browning reaction exhibited the strongest mutagenicity toward Salmonella typhimurium TA98 with S-9. Methylene chloride extract of pentose-creatine and pentose-glycine browning reaction solutions was also tested for mutagenicity, with positive responses. Methylene chloride extract of pentose-creatine-glycine browning reaction solutions induced significant increase in chromosome aberrations in the treated Chinese hamster ovary(CHO) cells. Each of pentose-creatine and pentose-glycine browning reaction solutions induced a relatively low frequency of chromosome aberrations in the treated cells.

• 생명과학회지
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• 제18권11호
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• pp.1499-1506
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• 2008

식용 및 약용으로 이용되는 산초(Zanthoxylum schinifolium)의 줄기로부터 항암활성 성분을 분리하기 위하여, 산초 줄기를 유기용매로 추출하고 각 추출물의 인체 급성백혈병 암세포에 대한 독성 및 세포자살 유도 활성을 조사하였다. Methanol (SS-7), methylene chloride (SS-8), ethyl acetate (SS-9), n-butanol (SS-10)로 추출한 각 시료와 유기용매 추출 후 잔여분획 (SL-14)의 세포 독성을 인체 급성백혈병 Jurkat T 세포주를 대상으로 조사한 결과, 암세포에 대한 세포독성이 주로 methylene chloride 추출분획인 (SS-8)에서 확인되었다. Methylene chloride 추출물 (SS-8)의 Jurkat T 세포주에 대한 세포독성의 기전은 mitochondria로부터cytochrome c 방출, caspase-9 및 caspase-3의 활성화, PARP 분해, internucleosomal DNA fragmentation 등의 일련의 생화학적 반응을 수반하며, 항 세포자살단백질인 Bcl-xL단백질의 과발현에 의해 억제되는 세포자살 기전임을 확인하였다. FADD가 disruption된 Jurkat T cell clone I2.1 ($FADD^{-/-}$) 및 caspase-8가 결핍된 Jurkat T cell clone I9.2 (caspase-$8^{-/-}$)와 함께 the wild-type Jurkat T cell clone A3에 미치는 SS-8의 세포독성작용을 비교 분석한 결과, wild-type Jurkat A3, FADD-deficient Jurkat clone I2.1및 caspase-8-deficient Jurkat clone I9.2 모두는 SS-8의 세포독성에 대해 유사한 정도의 감수성을 나타내었다. 이는 SS-8에 의해 유도되는 apoptosis에 있어서, Fas/FasL system이 관계되지 않음을 시사한다. 한편, SS-8를 GC-MS 분석하여, 9,12-octadecanoic acid (18.62%), 2,4-dihydro-5-methyl-4-(1-methylethylidene)-2-(4-nitrophenyl)- 3H-pyrazol-3-one (14.97%), hexadecanoic acid (14.23%), (z,z)-6,9-pentadecadien-1-ol (13.73%), 5,6-dimethoxy- 2-methyl benzofuran (10.95%), 그리고 4-methoxy-2-methylcinnamic acid (5.38%) 등을 포함한 16가지의 구성 성분과 그 조성비를 확인하였다. 이상의 연구결과는 산초 줄기에 함유된 항암 활성에 대한 규명과 이해를 증진시킨다.

• 생명과학회지
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• 제23권12호
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• pp.1460-1470
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• 2013

잡곡류의 항혈전작용을 조사하기 위해 국내산 11종의 잡곡으로부터 80% 에탄올 추출물을 얻어 혈액응고저해활성을 thrombin time (TT)법으로 측정한 결과, 황금찰수수의 에탄올 추출물이 가장 높은 혈액응고저해활성을 보였으며, 뒤이어 청차조 유래의 에탄올 추출물도 혈액응고저해활성을 보였다. 황금찰수수의 에탄올 추출물을 n-hexane, methylene chloride, ethyl acetate 및 n-butanol로 분획하였을 때, 대부분의 TT-저해활성은 hexane과 methylene chloride 분획에 분포하였으며, 이들 분획의 활성은 동일 농도의 aspirin (최종 농도 480 ${\mu}g/ml$)보다 더 높은 것으로 나타났다. 또한 황금찰수수의 에탄올 추출물의 혈액응고저해활성은 activated partial thromboplastin time (APTT)법 및 prothrombin time (PT)법으로도 확인되었다. 이때 APTT-저해활성은 hexane 및 methylene chloride분획에 주로 분포하였으나 PT-저해활성은 hexane 분획에 주로 분포하였다. 이들 분획들의 APTT-저해활성 및 PT-저해활성은 warfarin (최종농도 3.13 mg/ml) 보다 더 높은 것으로 확인되었다. 황금찰수수 유래 에탄올 추출물의 TT-저해활성은 열 안정성 및 pH 안정성이 매우 우수하였다. 한편, 이와 같은 황금찰수수 유래 에탄올추출물, hexane 분획 및 methylene chloride 분획의 경우는 피브린 응괴를 가용화할 수 있는 피브린용해활성을 지닌 것으로 나타났다. 이러한 결과들은 황금찰수수가 혈액응고저해활성과 혈전용해활성을 지니고 있음을 보여주며, 아울러 항혈전 식이요법의 소재가 될 수 있음을 시사한다.

• 생명과학회지
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• 제16권3호
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• pp.546-554
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• 2006

식용 및 약용으로 이용되는 산초 (Zanthoxylum schinifolium) 잎에 함유된 항암활성 성분을 분리하기 위하여 산초잎을 유기용매로 추출하여, 각 추출물의 암세포에 대한 독성 및 세포자살 유도 활성을 조사하였다. Methanol, methylene chloride, ethyl acetate, n-butanol로 추출한 각 추출물의 세포 독성을 인체 급성백혈병 Jurkat T 세포주, estrogen receptor-positive 유방암 세포주 MDA 361과 estrogen receptor-negative 세포주 MDA 438를 대상으로 조사한 결과, 이들 암세포주에 대한 세포독성이 methylene chloride 추출물 (SL-14)에서 주로 확인되었다. Methylene chloride 추출물 (SL-14)의 Jurkat T세포주에 대한 세포독성의 기전은 mitochondria로부터cytochrome c 방출, 이에 뒤이은 caspase-9 및 caspase-3의 활성화, PARP 분해, jnternucleosomal DNAfragmentation등의 일련의 생화학적 과정을 통해 유도되며 또한 Bcl-xL의 ectopic overexpression에 의해서는 negative regulation되는 세포자살임을 확인하였다. 또한 SL-14를 GC-MS 분석하여, 9,19-cyclolanost-24-en-3-ol (15.1%), 2-a-methyl-17, b-hop-21-ene (15.1%), 15-methyl-2,3-dihydro-1H benzazepin (11.95%), phytol (10.38%), lupeol (9.92%), 12-methylbenzofuran (8.23%) 등을 포함한 22가지의 구성성분과 그 조성비를 확인하였다. 이상의 연구결과들은 식용 산초잎에 함유된 항암 활성으로서의 세포자살유도 활성의 규명과 이해에 유익하게 활용될 것으로 기대된다.

• 한국식품영양학회지
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• 제29권6호
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• pp.946-951
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• 2016

In this study, Ilex serrata Thunb was extracted with 70% methanol and concentrated under reduced pressure to measure its total polyphenol contents; furthermore, we examined the effect of electron donating ability of the various extracts. The extract was fractionated using different solvents such as methylene chloride, ethyl acetate, and methanol to investigate total polyphenol contents and electron donating abilities. The total polyphenol contents were $1,058.59{\pm}85.85{\mu}g\;GAE/mL$ in 70% methanol, $297.29{\pm}29.43{\mu}g\;GAE/mL$ in methylene chloride, $313.28{\pm}9.22{\mu}g\;GAE/mL$ in ethyl acetate, and $837.82{\pm}31.16{\mu}g\;GAE/mL$ in methanol. The total polyphenol contents showed significant differences (p<0.05) between the solvents. The electron donating abilities were $72.93{\pm}0.29%$ for 70% methanol extract, $14.02{\pm}2.01%$ for methylene chloride, $24.05{\pm}1.75%$ for ethyl acetate, and $84.08{\pm}1.95%$ for methanol. The electron donating abilities were significantly different (p<0.05) between the solvents. The two portions of Ilex serrata Thunb extracts obtained from methanol showed significantly relevant results in the total polyphenol contents and electron donating ability, which was higher than the original extract.

• 한국자원식물학회지
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• 제5권1호
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• pp.49-56
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• 1992

In the preliminary antitumor screening tests of Crude Drugs and collected plants, the methanol ic extract of Forsythia Fruits in Korean marcket showed signif icant cytotoxic activity against Chinese hamster V-79 cells, but that in Japanese market did not. The former was identifid as Forsythia viridissima Lindley and latter as F.suspensa Vahl on the basis of the morphological observation. When an aqueous solution of the extract prepared from the fruits of F. viridissima (Oleaceae) was partitioned successively with n-hexane, methylene chloride, n-butanol, the cytoyoxic activitywas concetrated in the methylene chloride extract. Fractionation of the extract was made with the guidance of bioassay against V-79 cells to give cytotoxic lignans, matariresiol (1) and arctigenin (2). Their ICso values of compounds 1 and 2 were respectively $7.8{\;}\mu\textrm{g}/ml$ 1 and $1.65{\;}\mu\textrm{g}/ml$. Also, their structures were confirmed by comparison of physical and spectral data in the literature.

• 생명과학회지
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• 제18권2호
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• pp.167-174
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• 2008

치아우식균으로 알려져 있는 Streptococcus mutans에 대한 초피(Zanthoxylum piperitum)의 항균활성을 조사하기 위해, 뿌리, 줄기, 잎, 과피, 그리고 종자 부분을 methanol, ethyl acetate, hexane, methylene chloride등의 유기용매로 추출한 후 그 추출물의 항균활성을 $1{\sim}3$ mg/disc 농도범위에서 측정하였다. 그 결과, 종자의 경우만 활성이 나타났으며 특히 methylene chloride 추출물에서 비교적 높은 항균활성이 확인되었다. 초피종자의 항균활성이 종자의 정유성분을 추출할 경우에도 회수되는지를 조사하기 위해 초피종자 및 초피과피로부터 정유성분을 추출하고 이들의 S. mutans에 대한 항균활성을 측정하였다. 이때 두 정유성분 모두는 종자의 methylene chloride 추출물 보다 더 강한 항균효과를 보였다. 이 정유성분들의 S. mutans에 대한 최소저해농도(MIC)를 액체배양으로 측정한 결과, 초피종자 정유성분의 MIC는 0.3 mg/ml 로, 또한 초피과피 정유성분의 MIC는 4 mg/ml로 나타났다. S. mutans에 대한 항균활성이 가장 높게 나타난 종자의 정유성분을 박층크로마토그래피 방법으로 7가지 분획으로 나누어 그 항균효과를 서로 비교 조사한 결과, 다섯번째 분획(CS-SD-E)의 항균활성이 다른 분획들의 항균활성에 비해 상대적으로 높았으나, 분획 전의 정유성분에 비해서는 모두 항균활성이 낮았다. 초피종자의 항균활성성분을 조사하기 위해, 초피종자의 methylene chloride 추출물과 초피종자 정유성분을 GC-MS로 비교 분석하였다. 초피종자의 methylene chloride 추출물에서는 S. mutans에 대한 항균활성이 이미 보고된 물질로서 carvacrol (0.24%), ${\beta}$-caryphyllene (1.72%), ${\alpha}$-humulene (0.88%)등이 검출되었고, 초피종자 정유성분에서는 sabinene (1.57%), linalool (1.55%), citronellal (13.67%), terpinene-4-ol (0.45%), citronellol (3.69%), geraniol (0.9%), linalyl acetate (1.35%), ${\beta}$-caryophyllene (1.35%), ${\alpha}$-humulene (0.7818%) 및 ${\delta}$-cadinene (0.67%) 등이 S. mutans에 대한 항균활성이 보고된바 있는 물질로서 확인되었다. 이상의 연구결과는 초피종자가 S. mutans에 대해 항균활성을 가지며, 수증기 증류를 통해 확보하는 종자의 정유성분 회수가 이러한 항균물질을 추출하는 효과적인 방법임을 시사한다. 아울러 본 연구결과는 초피종자 유래의 정유성분이 S. mutans에 의해 발생하는 치아우식의 예방에 활용될 수 있음을 시사한다.

• 한국식품영양학회지
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• 제32권6호
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• pp.581-588
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• 2019

In this study, S. glauca (Bunge) Bunge extract was testified total polyphenol contents, electron donating ability on DPPH, and scavenging ability of superoxide anion radical and hydrogen peroxide. Total polyphenol contents of S. glauca (Bunge) Bunge extract were 7,053.30±915.93 ㎍ GAE/mL. In the fractions, fraction by ethanol was the highest content of 10,973.30±1,000.24 ㎍ GAE/mL. Fractions of ethyl acetate and water were 2,386.70±166.53 ㎍ GAE/mL, 2,413.30±41.63 ㎍ GAE/mL respectively. It was shown that total content of polyphenol according to solvent was significant relation at p<0.05. In the experiment of the electron donating ability, 70% ethanol extract and methylene chloride fraction were -246.15±24.17%, -254.01± 16.54% respectively. In case of ethyl acetate fraction, it was the highest electron donating ability to DPPH radical, 39.06±0.34%, and then water fraction of 36.71±5.55%, ethanol fraction of 29.77±2.57%, gradually. Electron donating abilities revealed significant difference (p<0.05) between the solvents. The superoxide radical scavenging ability of standard material was 0.029±0.0011; 70% ethanol extract of 0.022±0.00052, methylene chloride fraction of 0.027±0.00031, ethyl acetate fraction of 0.024±0.0011, ethanol fraction of 0.021±0.00024 and water fraction of 0.024±0.00019. Hydrogen peroxide scavenging ability of 70% ethanol extract was -0.0029±0.00040 and the others were as follow; methylene chloride fraction (-0.0042±0.00058), ethyl acetate fraction (-0.003± 0.0041), ethanol fraction (-0.0029±0.0015) and water fraction (-0.0028±0.00090).