• Microbiology and Biotechnology Letters
• /
• v.27 no.3
• /
• pp.252-259
• /
• 1999

Anoxic sediments collected from Shiwha-ho area were used to find the relationship between the heavy-metal, organic content and anaerobic respiration bacteria by most probable number (MPN) method. Analysis of the sediments showed that COD content was higher in the sediments collected from Ansan-cheon and Shiwha-ho than those collected from sea area nearby. Particularly noticeable was the fact that heavy metal concentration was much higher in the sediments of Shiwha-ho area contaminated by heavy-metal, although they were rich in electron donor and electron acceptor for Fe(III)-reducing bacteria using lactate as an electron donor was in the range of 1.1$\times$106-4.6$\times$107MPNs/ml in the sediments collected from the sea-side of the lake, which were lower in heavy-methal concentration and higher in Fe-Mn content than those from other region. The number of Fe(III)-reducing bacteria using acetate as an electron donor was in the rang eof 4.3$\times$102-8.1$\times$105MPNs/ml in the same sediments. Chromate-reducing bacteria were more populated(4.6$\times$104-8.1$\times$105MPNs/ml) in the sediments contaminated by heavy metals. The number of sulfate-reducing bacteria wee counted in the sediments collected from the more contaminate inner-side than those from the sea-side of the lake.

• Journal of the Mineralogical Society of Korea
• /
• v.15 no.3
• /
• pp.231-240
• /
• 2002

Microbial metal reduction influences the biogeochemical cycles of carbon and metals as well as plays an important role in the bioremediation of metals, radionuclides, and organic contaminants. The use of bacteria to facilitate the production of magnetite nanoparticles and the formation of carbonate minerals may provide new biotechnological processes for material synthesis and carbon sequestration. Metal-reducing bacteria were isolated from a variety of extreme environments, such as deep terrestrial subsurface, deep marine sediments, water near Hydrothemal vents, and alkaline ponds. Metal-reducing bacteria isolated from diverse extreme environments were able to reduce Fe(III), Mn(IV), Cr(VI), Co(III), and U(VI) using short chain fatty acids and/or hydrogen as the electron donors. These bacteria exhibited diverse mineral precipitation capabilities including the formation of magnetite ($Fe_3$$O_4$), siderite ($FeCO_3$), calcite ($CaCO_3$), rhodochrosite ($MnCO_3$), vivianite [$Fe_3$($PO_4$)$_2$ .$8H_2$O], and uraninite ($UO_2$). Geochemical and environmental factors such as atmospheres, chemical milieu, and species of bacteria affected the extent of Fe(III)-reduction as well as the mineralogy and morphology of the crystalline iron mineral phases. Thermophilic bacteria use amorphous Fe(III)-oxyhydroxide plus metals (Co, Cr, Ni) as an electron acceptor and organic carbon as an electron donor to synthesize metal-substituted magnetite. Metal reducing bacteria were capable of $CO_2$conversion Into sparingly soluble carbonate minerals, such as siderite and calcite using amorphous Fe(III)-oxyhydroxide or metal-rich fly ash. These results indicate that microbial Fe(III)-reduction may not only play important roles in iron and carbon biogeochemistry in natural environments, but also be potentially useful f3r the synthesis of submicron-sized ferromagnetic materials.

• KSBB Journal
• /
• v.29 no.3
• /
• pp.139-144
• /
• 2014

Microorganisms play a significant role in bioremediation of heavy metal contaminated seawater. In this study, we reported an effective removal of Cu and Zn in marine envionment by using Desulfovibrio desulfuricans (D. desulfuricans) which belong to sulfate reducing bacteria. D. desulfuricans showed stable growth characteristics in high salt concentration and had resistance to heavy metals. Cu and Zn was removed not only by physical adsorption on the surface of bacteria but also by precipitation reaction of microbial metabolism by D. desulfuricans in seawater. In case of different heavy metal concentration, Cu was effectively removed 85% at 25 ppm and 60% at 50 ppm and Zn was effectively removed 54% at 50 ppm and 46% at 200 ppm, respectively.

• Journal of the Mineralogical Society of Korea
• /
• v.24 no.4
• /
• pp.279-287
• /
• 2011

In order to identify if bacteria surviving in soils and groundwater can change the oxidation/reduction potential of groundwater, Eh values of solution that contained bacteria were measured for 2 weeks. The Eh values of the solution reacted with sulfate-reducing bacteria decreased from -120 mV to -500 mV in 5 days, and $Desulfuricans$ was superior to $Vulgaris$ in reducing the solution. The Eh value was relatively higher for the solution containing $Shewanella$, iron-reducing bacteria, showing -400 mV. During the Eh decrease by the metal-reducing bacteria, a sulfide mineral such as mackinawite (FeS) started precipitating through the microbial reducing process for sulfate and ferric iron. These results show that the ORP of natrual groundwater may be sensitive to the geomicrobial respiration. In addition, a subsurface environment where groundwater is highly reduced and sulfide minerals are largely biogenerated may be a good place to retard the migration of oxidized radionu-clides by making them precipitated as reduced forms.

• Journal of Korean Society of Environmental Engineers
• /
• v.30 no.8
• /
• pp.798-807
• /
• 2008

Due to economic impairment derived from metal corrosion of pumping station installed around coastal area, it was needed for related cause-effect to be investigated for understanding practical corrosion behavior and providing proper control. This research was thus carried out to determine whether the microbe can influence on metal corrosion along with its control in the laboratory. For this study, groundwater was sampled from the underground pump station(i.e. I Gas Station) where corrosion was observed. Microbial diversity on the samples were then obtained by 16S rDNA methods. From this, microbial populations showing corrosion behaviors against metals were reported as Leptothrix sp.(Iron oxidizing) and Desulfovibrio sp.(Sulfur reducing) Iron oxidizing bacteria were dominantly participating in the corrosion of iron, while sulfate reducing bacteria were more preferably producing precipitate of iron. In case of galvanized steel and stainless steel, iron oxidizing bacteria not only enhanced the corrosion, but also generated its scale of precipitate. Sulfate reducing bacteria had zinc steel corroded greater extent than that of iron oxidizing bacteria. In the inactivation test, chlorine or UV exposure could efficiently control bacterial growth. However as the inactivation intensity being increased beyond a threshold level, corrosion rate was unlikely escalated due to augmented chemical effect. It is decided that microbial corrosion could be differently taken place depending upon type of microbes or materials, although they were highly correlated. It could be efficiently retarded by given disinfection practices.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2008.05a
• /
• pp.82-85
• /
• 2008

• Journal of Microbiology and Biotechnology
• /
• v.30 no.7
• /
• pp.1005-1012
• /
• 2020

Acid mine drainage (AMD) has been a serious environmental issue that threatens soil and aquatic ecosystems. In this study, an acid-tolerant sulfate-reducing bacterium, strain S4, was isolated from the mud of an AMD storage pond in Vietnam via enrichment in anoxic mineral medium at pH 5. Comparative analyses of sequences of the 16S rRNA gene and dsrB gene involved in sulfate reduction revealed that the isolate belonged to the genus Desulfovibrio, and is most closely related to Desulfovibrio oxamicus (with 99% homology in 16S rDNA sequence and 98% homology in dsrB gene sequence). Denaturing gradient gel electrophoresis (DGGE) analyses of dsrB gene showed that strain S4 represented one of the two most abundant groups developed in the enrichment culture. Notably, strain S4 was capable of reducing sulfate in low pH environments (from 2 and above), and resistance to extremely high concentration of heavy metals (Fe 3,000 mg/l, Zn 100 mg/l, Cu 100 mg/l). In a batch incubation experiment in synthetic AMD with pH 3.5, strain S4 showed strong effects in facilitating growth of a neutrophilic, metal sensitive Desulfovibrio sp. strain SR4H, which was not capable of growing alone in such an environment. Thus, it is postulated that under extreme conditions such as an AMD environment, acid- and metal-tolerant sulfate-reducing bacteria (SRB)-like strain S4 would facilitate the growth of other widely distributed SRB by starting to reduce sulfate at low pH, thus increasing pH and lowering the metal concentration in the environment. Owing to such unique physiological characteristics, strain S4 shows great potential for application in sustainable remediation of AMD.

• Journal of Plant Biotechnology
• /
• v.3 no.1
• /
• pp.13-17
• /
• 2001

A Chromium (VI)[Cr(VI)] reductase gene from heavy metal reducing bacteria Pseudomonas aeruginosa HP014 was used to transform tobacco plant cells. A chimeric construct containing the Cr(VI) reductase gene was transfered to tobacco leaf disks using an Agrobacteriun tumefaciens binary vector system. From the leaf disks, transformed plantlets were regenerated. Hybridization experiments demonstrated that the Cr(VI) reductase gene was inserted into and expressed in the regenerated plants. The Cr(VI) reduction activity showed that the transgenic plants may be a another possible tool to reduce the pollution of the toxic Cr(VI) in soil.

• Journal of the Mineralogical Society of Korea
• /
• v.16 no.2
• /
• pp.171-180
• /
• 2003

The objective of this study is to investigate biogeochemical processes to sequester $CO_2$and metals utilizing metal-rich fly ash (MRFA). Microbial conversion of $CO_2$into sparingly soluble carbonate minerals has been studied using MRFA under different $pCO_2$and different bicarbonate concentrations. Scaling from test tube to fermentation vessels (up to 4-L) using metal-reducing bacteria and MRFA has proved successful at sequestering carbon dioxide and metals. $CO_2$sequestration via precipitation processes using MRFA may complement the process of $CO_2$capture from fossil fuel plants while potentially stabilizing fly ash wastes.

• Korean Journal of Materials Research
• /
• v.32 no.3
• /
• pp.168-179
• /
• 2022

Microbiologically Influenced Corrosion (MIC) occurring in underground buried pipes of API 5L X65 steel was investigated. MIC is a corrosion phenomenon caused by microorganisms in soil; it affects steel materials in wet atmosphere. The microstructure and mechanical properties resulting from MIC were analyzed by OM, SEM/EDS, and mapping. Corrosion of pipe cross section was composed of ① surface film, ② iron oxide, and ③ surface/internal microbial corrosive by-product similar to surface corrosion pattern. The surface film is an area where concentrations of C/O components are on average 65 %/16 %; the main components of Fe Oxide were measured and found to be 48Fe-42O. The MIC area is divided into surface and inner areas, where high concentrations of N of 6 %/5 % are detected, respectively, in addition to the C/O component. The high concentration of C/O components observed on pipe surfaces and cross sections is considered to be MIC due to the various bacteria present. It is assumed that this is related to the heat-shrinkable sheet, which is a corrosion-resistant coating layer that becomes the MIC by-product component. The MIC generated on the pipe surface and cross section is inferred to have a high concentration of N components. High concentrations of N components occur frequently on surface and inner regions; these regions were investigated and Na/Mg/Ca basic substances were found to have accumulated as well. Therefore, it is presumed that the corrosion of buried pipes is due to the MIC of the NRB (nitrate reducing bacteria) reaction in the soil.