• Title/Summary/Keyword: metal chelating activity

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Antioxidant and Antidiabetic Activities of Aralia elata Seeds

  • Hu, Weicheng;Jung, Mee-Jung;Heo, Seong-Il;Wang, Myeong-Hyeon
    • Journal of Applied Biological Chemistry
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    • v.51 no.3
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    • pp.111-116
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    • 2008
  • Aralia elata seeds were successively extracted with water, methanol, ethanol, acetone and chloroform. The crude extracts were investigated for antioxidant and antidiabetic activities. The antioxidant properties of various extracts were evaluated by antioxidant tests, such as DPPH free radical-scavenging activity, hydroxyl radical-scavenging assay, metal-chelating activity, lipid peroxidation inhibition activity and reducing power assay. The 70% methanol extract exhibited the highest activity in the in vitro models of DPPH free radical-scavenging activity, metal-chelating activity, and reducing power assay. Acetone extract showed good effects on lipid peroxidation inhibition and hydroxyl radical-scavenging assay at a low concentration. In addition, the $\alpha$-glucosidase inhibition assay showed that 70% methanol extract had the highest activity. These results indicate the high possibility of using A. elata seeds for medical application due to their efficient antioxidant properties.

A Study on Antioxidant Activity of Bread with Waxy Black Rice Flour Added (찰흑미를 첨가한 식빵의 항산화성에 관한 연구)

  • Kim, Weon-Mo;Lee, Yoon-Shin
    • Culinary science and hospitality research
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    • v.13 no.4
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    • pp.178-185
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    • 2007
  • The antioxidant activities of the bread made from waxy black rice flour(WBRF) and wheat flour were investigated. The waxy black rice bread was manufactured with 10, 20, 30, or 40% waxy black rice flour(w/w). As the additive amount of WBRF were increased, DPPH(1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity and TEAC(trolox equivalent antioxidant capacity) were also increased. WBRF group showed the higher activity than the control group in DPPH radical scavenging activity and TEAC. The group with 10% WBRF added was not significantly different from the control group in metal chelating activity, but over 20% of addition, it had the higher metal chelating activity than that of the control group.

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Radical Scavenging and Anti-inflammation Activities from Different Extracts of Zanthoxylum schnifolium Fruits (추출용매에 따른 산초 (Zanthoxylum schnifolium)열매 추출물의 라디칼소거능과 항염증 효과)

  • Han, Woong;Wang, Myeong-Hyeon
    • Korean Journal of Pharmacognosy
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    • v.41 no.4
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    • pp.250-254
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    • 2010
  • This study was presented to the physiological activities such as metal chelating capacity, superoxide dismutase-like activity, anti-inflammation, cytotoxicity from Zanthoxylum schnifolium fruit. The values for metal chelating capacity of 1 mg/mL of 70% EtOH and 70% MeOH extracts were 68% and 67%, respectively, it was shown that metal chelating capacity in a dose-dependent manner. The 100% EtOH and 100% MeOH extract exhibited strong superoxide dismutase-like activity. The highest SOD-like activity obtained from 100% EtOH was 51% at a concentration of 4 mg/mL. The all extracts of Zanthoxylum schnifolium fruit except $H_2O$ extract significantly decreased the concentration of LPS-induced NO in Raw 264.7 cells. The 100% EtOH, 100% MeOH and 70% EtOH extract represented the highest activity in the anti-inflammation properties in vitro. However, the 100% EtOH and 100% MeOH extracts has cytotoxicity in Raw 264.7 cells and it may affect cell viability. Conclude that 70% EtOH extract was most suitable for anti-inflammation. Our results revealed that the Zanthoxylum schnifolium fruit expected to physiological activities.

Immobilization of Heavy metal mechanism in Contaminated Coastal Sediment using Biostimulant Ball (BSB) with Modified Zeolite

  • Subha, Bakthavachallam;Woo, Jung-Hui;Song, Young-Chae
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2016.05a
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    • pp.130-131
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    • 2016
  • Although many metals contaminated sediment from coastal area contain both anionic and cationic heavy metals, the current remediation technologies are not effective for stabilize heavy metals of both anionic and cationic elements from contaminated coastal region. the present work investigated the efficiency and mechanism of immobilization of Fe, Zn, Cr, Cu, Pb and Cd metal solutions in modified zeolite based biostimulant ball. Biostimulant ball containing acetate, nitrate and sulphate which are enhance the activity of marine microorganisms and it can act as electron donors and electron acceptors. Modified zeolite and chelating agent is greatly enhance the metal stabilization due to increased immobility of the analysed metals. The XRD, FT-IR and SEM of modified zeolite which cheating agents containing heavy metals were investigated. The results indicated that heavy metals could be effectively immobilized in modified zeolite and chelating agents in BSB added sediment. The immobilization of heavy metals in modified zeolite and chelating agents along with BSB could be due to stabilize of heavy metal cations. Immobilization of heavy metals using BSB with modified zeolite and chelating agent has lower cost effect and enhance the sediment quality.

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Effect of Red Pepper Seed on Kimchi Antioxidant Activity During Fermentation

  • Sim, Ki-Hyeon;Han, Young-Sil
    • Food Science and Biotechnology
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    • v.17 no.2
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    • pp.295-301
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    • 2008
  • In this study, the antioxidant activities of red pepper seed kimchi extracts were examined. The extracts were evaluated by various antioxidant assays that included determinations of total phenolic and flavonoid contents, DPPH radical scavenging, superoxide anion radical scavenging, nitric oxide scavenging, superoxide dismutase (SOD) activity, metal chelating activity, and reducing power. All the kimchi extracts showed strong antioxidant activities by the tested methods. The 7% red pepper seed kimchi that was fermented for 6 days possessed the highest activity according to the performed tests. Yet, the highest scavenging activity was exhibited against nitrite, by extracts from kimchi for 0 day of fermentation rather than 6 days. All the activities for the kimchi fermented for 0 day with the addition of 7% red pepper seed increased markedly with an increase in concentration. With the exception of metal chelating and SOD activities, for the antioxidant properties assayed, the red pepper seed kimchi extracts were found to be highly effective.

Physiological Activity of Acai Berry (Euterpe oleracea Mart.) Extracted with Different Solvents (추출용매에 따른 아사이 베리의 생리활성)

  • Chung, Hai-Jung
    • Journal of the Korean Society of Food Culture
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    • v.27 no.1
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    • pp.75-81
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    • 2012
  • This study was conducted to investigate the physiological activity of acai berry (Euterpe oleracea Mart.) extracts from three different solvents (water, methanol, and ethanol). We measured total polyphenol and total flavonoid content, DPPH radical scavenging activity, nitrite scavenging activity, metal chelating effect, and reducing power. The extraction yield from water, methanol, and ethanol was 17.10, 9.50, and 37.51%, respectively. The highest total polyphenol content (10.54 mg/100 g) and total flavonoid contents (1.88 mg/100 g) was observed in water extract. DPPH radical scavenging activity was the highest in both water extract (72.03%) and methanol extract (74.79%) at levels of 5 mg/mL, which was similar to that of BHT (78.90%). Water extract yielded the highest metal chelating effect (92.54%) and reducing power (1.09) at levels of 5 mg/mL. Taken together, these findings suggest that extracts of acai berry can be used as functional food materials with antioxidative and nitrite scavenging activities.

Evaluation of Antioxidative activity of Korean Yam (Dioscorea batatas DECNE.) by n-Butanol and Ethyl Acetate Extracts

  • Duan, Yishan;Kim, Han-Soo;Kim, Gyeong-Hwuii
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.2
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    • pp.312-319
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    • 2015
  • In this study, n-butanol and ethyl acetate extracts were prepared from raw yam (Dioscorea batatas DECNE.). Their antioxidative potencies were investigated employing various in vitro methods, such as ferrous ion chelating, ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and nitric oxide (NO) radical scavenging and nitrite scavenging activity. The n-butanol fraction was assayed to possess stronger antioxidant activity by ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and NO radical scavenging activity. However, ethyl acetate extract was more effective in chelating ferrous ion and scavenging nitrite. Based on the results obtained, yam is a potential active ingredient that could be applied in antioxidation as well as bio-health functional food to take a good part in prevention of human diseases and aging.

Evaluation of Antioxidant Activity of Essential Oil from Artemisia vulgaris

  • Bhatt, Lok Ranjan;Lee, Jae-Sug;Baek, Seung-Hwa
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.2
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    • pp.528-531
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    • 2007
  • Artemisia vulgaris essential oil exhibited a strong metal chelating activity but withlow reducing power, and radical scavenging activity. However, a gradual increase in the radical scavenging activity was obtained with increasing concentration and reaction time.

Free Radical Scavenging Activity and Protective Ability of Methanolic Extract from Duchesnea indica Against Protein Oxidation and DNA Damage

  • Hu, Weicheng;Shen, Wei;Wang, Myeong-Hyeon
    • Preventive Nutrition and Food Science
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    • v.14 no.4
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    • pp.277-282
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    • 2009
  • The antioxidant potency of methanolic extract of Duchesnea indica (MDI; Indian strawberry) was investigated by employing various established in vitro systems, such as total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power assay, metal chelating assay, superoxide radical scavenging activity and protective ability of DNA damage and protein oxidation. MDI inhibited metal chelating by 75.57% at 2 mg/mL, scavenged 50% DPPH free radical at 29.13 ${\mu}$g/mL, and eliminated approximately 46.21% superoxide radical at the concentration of 1 mg/mL. In addition, MDI showed strong ability on reducing power, DNA damage protection and protein oxidation protection. Overall, results suggested that MDI might be beneficial as a potent antioxidant and effectively employed as an ingredient in food applications.

Studies on Preparation of Resion-Metal Chelates and Its Catalytic Activity for the Oxidation of Hydroxy Compounds and l-Ascorbic Acid (Resin-Metal Chelate의 제조와 Hydroxy화합물 및 l-Ascorbic Acid의 산화 촉매효과에 관한 연구)

  • Whang Kyu-Ja;Lee Young Sun;Kim Young Mi;Lee Yong-Keun
    • Journal of the Korean Chemical Society
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    • v.33 no.1
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    • pp.113-119
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    • 1989
  • Chelating resins containing hydrazide or triethylenetetramine side chain were prepared using a commercial cation exchage resin, Diaion WK 11, and their nitrogen contents were determined by elemental analysis. The synthesized resin, and commercial chelating resins, (Diaion-CR 10 and-CR 20) were treated with various metal chelates of which metal contents were subsequently determined by chelatometry. Sectioned beads of the resin-metal chelates were also observed using electron microprobs X-ray analyzer. To examine the catalytic activity of the resin-metal chelates, they were applied to the oxidation of various hydroxy compounds and l-ascorbic acid, and found to be effective catalysts.

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