• 한국환경농학회지
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• 제24권4호
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• pp.379-385
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• 2005

중금속으로 오염된 폐수를 처리하기 위하여 다양한 처리 방법들이 제시되고 있으나, 최근에는 미생물을 이용한 중금속 처리 방법에 대한 관심이 높아지고 있다. 따라서 중금속으로 오염된 토양에서 중금속에 대하여 강한 내성과 우수한 생물흡착능력을 동시에 가지고 있는 미생물 CPB를 분리하여 중금속에 대한 내성, 흡착 능력 및 흡착의 최적조건을 조사하였다. 중금속에 대한 내성은 전체적으로 $400mg{\ell}^{-1}$ 이상의 농도에서도 높은 내성을 보였으며, 생장 저해도는 단일 중금속 보다 중금속이 복합으로 존재 할 경우 더 크게 나타났다. 중금속 흡착 능력은 Pb>Cd>Cu>Zn의 순으로 흡착 능력이 나타났다. 외형적 흡착형태는 중금속의 종류에 따라 세포 표면 및 세포 막 부근에 electron dense particles들이 형성되었으며, 이는 EDS 분석을 통하여 중금속 화합물인 것으로 확인되었다. 중금속을 흡착하는데 있어 전반적인 최적 pH는 $5{\sim}7$범위였고, 최적 온도는 $25{\sim}35^{\circ}C$이다.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.607-611
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• 2004

Glycinin, one of the predominant storage proteins in soybeans, was examined as to whether it could be used as a calcium-binding mediator after chemical phosphorylation and enzymatic hydrolysis. Glycinin is composed of six subunits. One of the proglycinin subunits $(A_{la}B_{lb})$ was overexpressed in E. coli to obtain nonphosphorylated proteins with homogeneity. To investigate the enhanced calcium-binding properties of the phosphopeptides, the proglycinin was purified, phosphorylated, and hydrolyzed with trypsin. The proglycinin expressed in E. coli was purified by ammonium sulfate precipitation, ion-exchange chromatography, and cryoprecipitation. Chemical phosphorylation by sodium trimetaphosphate was performed to obtain phosphorylated proglycinin. After the phosphorylation, one-dimensional isoelectric focusing gel electroanalysis confirmed the phosphorylation of the proglycinin. The phosphorylated peptides were then hydrolyzed with trypsin, followed by a binding reaction with calcium chloride. The calcium-bound phosphopeptides were finally separated using immobilized metal $(Ca^{2+})$ chromatography. Consequently, a limited tryptic hydrolysate of the isolated phosphopeptides exhibited an enhanced calcium-binding ability, suggesting the potential of glycinin phosphopeptides as a calcium-binding mediator with greater availability.

• 분석과학
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• 제8권3호
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• pp.259-264
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• 1995

수용액서 poly-NTOE(1, 12-diaza-3, 4:9, 10-dibenzo-5, 8-dioxacyclopentadeca-1, 12-ylene-2, 7-dihydroxyoctamethylene)를 흡착시킨 실리카겔을 고정상으로 하는 액체 크로마토그래피를 이용하여 혼합 금속이온용액으로부터 전이금속 및 후전이금속이온들의 분리효율을 결정하였다. pcly-NTOE와 금속이온들간의 결합상수 및 분리인자들의 순위는 전이금속이온의 경우 Co(II)Zn(II)였으며, 후전이금속이온들의 경우 Cd(II)

• Bulletin of the Korean Chemical Society
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• 제32권spc8호
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• pp.2928-2932
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• 2011

A chemosensor based on a rhodamine-hydroxamate platform containing a pyridine and a cyclen binding units has been developed for the detection of $Cd^{2+}$ in aqueous solutions. The probe responds selectively toward $Cd^{2+}$ over other biologically relevant metal ions. The fluorescent probe shows 1:1 binding stoichiometry and the detection limit for $Cd^{2+}$ in water proved to be as low as 25 nM.

• 약학회지
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• 제39권4호
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• pp.380-384
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• 1995

The isoelelectric points of Cd-BP(l) and Cd-BP(II), cadmium-binding proteins, were 6.01 and 5.35, respectively. Both of them contained zinc. As for the amino acid composition, Cd-BP(I) contained a lot of glycine and lysine but none of such aromatic amino acids as tyrosine and phenylalanine.. On the other hand, Cd-BP(II) contained leucine, histidine, asparti cacid and alanine but no aromatic amino acids.

• Bulletin of the Korean Chemical Society
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• 제29권1호
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• pp.234-236
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• 2008

• 한국진공학회:학술대회논문집
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• 한국진공학회 2008년도 제34회 동계학술대회 초록집
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• pp.114-114
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• 2008

• 한국분말야금학회:학술대회논문집
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• 한국분말야금학회 1995년도 추계학술강연 및 발표대회 강연 및 발표논문 초록집
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• pp.34-34
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• 1995

• Archives of Pharmacal Research
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• 제12권2호
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• pp.119-124
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• 1989

Cotton xanthate, which was obtained by treating cotton with carbon disulfide in alkaline solution, was treated with the solution of polyvalent metal ions to produce cotton xanthate-metal chelates. This chelation reaction was readily and simply achieved, and antimicrobial agents with suitable structures could subsequently be coupled to the chelate with ease at moderate pH values and in aqueous solution. Metal ions used in present work include Cu(II), Zn(II) and Fe(III). Tetracycline, streptomycin, neomycin and pyrithion were used as antimicrobial/antifungal agents. Antibacterial activities were measured employing ditch plate method against G(+) Staphylococcus aureus, Streptococus faecalis, and G(-) Escherichia coli, Enterobacter aerogenes, and the fungus, Aspergillus niger. All the cotton xanthate-metal-antimicrobial agent chelates exhibited activities whereas the cotton xanthate-metal chelates themselves were inactive. Considering the extensive washing procedures and results from control experiments, possibility of the involvement of physical adsorption for the binding of drugs could be excluded.

• Bulletin of the Korean Chemical Society
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• 제32권11호
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• pp.4021-4026
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• 2011

Functions of the luteinizing hormone releasing hormone (LHRH) and its induced release by divalent metal ions have received great attention because this neurotransmitter subsequently regulates the secretion of luteinizing hormone (LH). Metal-LHRH complexes were synthesized by addition of various Cu(II),Ni(II),Zn(II) ions into LHRH in order to understand how the induced release of LHRH is possible. The degree of complexation was monitored by $^1H$, $^{13}C$-NMR chemical shifts, and final products were identified by Mass spectrometry. Solutionstate structure determination of Zn(II)-LHRH out of metal-complexes was accomplished by using NMR and NMR-based distance geometry (DG). Interproton distance information from nuclear Overhauser effect spectroscopy was utilized for structure determination. Structure obtained in this study has a cyclic conformation exhibiting a specific ${\alpha}$-helical turn with residue numbers His[2]-Leu[7] out of 10 amino acids. Comparison of chemical shifts and EPR studies of Ni(II),Cu(II)-LHRH complexes exhibit that these metal complexes have 4-coordination geometry.