Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Biosorption of Heavy Metal in Aqueous Solution by Heavy Metal Tolerant Microorganism Isolated from Heavy Metal Contaminated Soil

중금속으로 오염된 토양에서 분리한 중금속 내성 미생물의 수용액내 중금속 흡착

  • Kim, Sung-Un (Division of Applied life and Environmental Sciences, Sunchon National University) ;
  • Choi, Ik-Won (Department of Chemical Engineering, Osaka Prefecture University) ;
  • Seo, Dong-Cheol (Division of Applied Life Science, Gyeongsang National University) ;
  • Han, Myung-Hoon (Division of Applied life and Environmental Sciences, Sunchon National University) ;
  • Kang, Byung-Hwa (Division of Applied life and Environmental Sciences, Sunchon National University) ;
  • Heo, Jong-Soo (Division of Applied Life Science, Gyeongsang National University) ;
  • Shon, Bo-Kyoon (Division of Applied life and Environmental Sciences, Sunchon National University) ;
  • Cho, Ju-Sik (Division of Applied life and Environmental Sciences, Sunchon National University)
  • 김성은 (순천대학교 생명환경과학부) ;
  • 최익원 (오사카부립대학 회학공학과) ;
  • 서동철 (경상대학교 응용생명과학부) ;
  • 한명훈 (순천대학교 생명환경과학부) ;
  • 강병화 (순천대학교 생명환경과학부) ;
  • 허종수 (경상대학교 응용생명과학부) ;
  • 손보균 (순천대학교 생명환경과학부) ;
  • 조주식 (순천대학교 생명환경과학부)
  • Published : 2005.12.31
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Abstract

This study was conducted to find out a useful bioremediation technology for heavy metal contaminated soil and water. We isolated strain CPB from heavy metal contaminated soil and evaluated the tolerance level and adsorption capacity of strain CPB to heavy metals (Strain is not determined yet). Strain CPB showed variable tolerance limit to different kinds heavy metal or concentrations of heavy metals. The growth of strain CPB was significantly inhibited by mixed heavy metals (Cd+Cu+Pb+Zn) than that of by single heavy metal. Strain CPB showed high binding capacity with Pb (Pb>Cd>Cu>Zn). In general, strain CPB showed high uptake of heavy metals such as Pb, Cd and Cu. It was observed that the capacity of heavy metal uptake from mixture of heavy metals was reduced in comparison with single heavy metal treatment. But total contents of heavy metal bound with cell in mixed heavy metal showed higher than in single heavy metal treatment. Heavy metal adsorption in cells was affected by several external factors, such as temperature and pH etc.. The optimum temperature and pH of the adsorption of heavy metal into cells were ca. $25{\sim}35^{\circ}C$ and pH ca. $5{\sim}7$, respectively. A large number of the electron dense particles were found mainly on the cell wall and cell membrane fractions, which was determined by transmission electron microscope. Energy dispersive X-ray spectroscopy revealed that the electron dense particles were the heavy metal complexes the substances binding with heavy metals.

중금속으로 오염된 폐수를 처리하기 위하여 다양한 처리 방법들이 제시되고 있으나, 최근에는 미생물을 이용한 중금속 처리 방법에 대한 관심이 높아지고 있다. 따라서 중금속으로 오염된 토양에서 중금속에 대하여 강한 내성과 우수한 생물흡착능력을 동시에 가지고 있는 미생물 CPB를 분리하여 중금속에 대한 내성, 흡착 능력 및 흡착의 최적조건을 조사하였다. 중금속에 대한 내성은 전체적으로 $400mg{\ell}^{-1}$ 이상의 농도에서도 높은 내성을 보였으며, 생장 저해도는 단일 중금속 보다 중금속이 복합으로 존재 할 경우 더 크게 나타났다. 중금속 흡착 능력은 Pb>Cd>Cu>Zn의 순으로 흡착 능력이 나타났다. 외형적 흡착형태는 중금속의 종류에 따라 세포 표면 및 세포 막 부근에 electron dense particles들이 형성되었으며, 이는 EDS 분석을 통하여 중금속 화합물인 것으로 확인되었다. 중금속을 흡착하는데 있어 전반적인 최적 pH는 $5{\sim}7$범위였고, 최적 온도는 $25{\sim}35^{\circ}C$이다.

Keywords

References

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