• Applied Biological Chemistry
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• v.49 no.4
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• pp.259-265
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• 2006

Effect of the fungicide mepanipyrim on the resistant and sensitive isolates of Botrytis cinerea was studied in vitro and also tested to control Botrytis rot of strawberry, cucumber and grape in the field. These isolates were selected by relative mycelial growth and spore germination on potato dextrose agar(PDA) incorporated with $100{\mu}g\;a.i./ml$ of benomyl and procymidone, respectively, compared to the unamended PDA. Mycelial growth of the selected resistant isolates was significantly inhibited by mepanipyrim but the inhibition rate was similar to other fungicides belong to benzimidazole or dicarboximide, although spore germination was not inhibited even by the higher concentration of mepanipyrim. When the benomyl and procymidone resistant isolates were inoculated to cucumber leaves, lesion development was significantly inhibited with application of $250{\mu}g\;a.i./ml$ of mepanipyrim but not with that of benomyl and procymidone. In addition, when $250{\mu}g\;a.i./ml$ of mepanipyrim was applied to strawberry, cucumber, and grape in the field, the control of Botrytis rot was significantly different from that of the untreated control(Duncan's multiple range test, p<0.05). The results suggested that mepanipyrim might be an alternative fungicide for the control of benomyl- and procymidone-resistant pathogens of Botrytis rot.

• The Korean Journal of Pesticide Science
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• v.19 no.3
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• pp.329-334
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• 2015

With 86 isolates of Botrytis cinerea obtained from diseased fruits of tomato, strawberry and cucumber, it was conducted to detect the fungicide resistance of B. cinerea against mepanipyrim through an agar dilution method. FGA medium was used for monitoring the resistance. Among 86 isolates of B. cinerea, resistant isolates were composed by 23.3%, which was different according to regions isolating the pathogen. In accordance with isolation region, the isolation frequency of resistant isolates was as follows; that of Gyeonggi, Gyeongnam/Pusan and chungnam was 28.6%, 33.3% and 12.8%, respectively. The resistant isolates of B. cinerea showed the cross resistance between mepanipyrim and pyrimethanil. Also the pathogenicity of B. cinerea resistant to mepanipyrim was high as like that of sensitive isolates. Because of them, it should be necessary to manage the spraying system of mepanipyrim and the monitoring for the fungicide resistance.

• The Plant Pathology Journal
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• v.17 no.6
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• pp.367.2-367
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• 2001

• Journal of Applied Biological Chemistry
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• v.52 no.3
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• pp.126-132
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• 2009

In order to elucidate the relationship between the rainfastness of fungicides and their water solubilities, the fungicide residues on the leaf surface of hot pepper was assessed and compared after the drop-wise applications of fungicide solutions on leaf surface followed by artificial raining. As the raining was progressed after application of aqueous acetone solution of fungicides, the residue levels of fungicides were drop rapid at the early stage of raining, but the decreasing rates of residue level were slowed down thereafter. The initial rainfastness was reversely proportional to the water solubilities of the fungicides. Whole amount of dimethomorph residue, which water solubility is 18 mg/L, was washed off by 2.5 mm of raining. Although WP formulations of fungicides showed remarkable decreases of rainfastness compared to the aqueous acetone formulations, the fungicides having low water solubility showed better rainfastness. Chlorothalonil and mepanipyrim suspension concentrates was better in rainfastness than their WP formulation, and the rainfastness of mepanipyrim suspension was reversely proportional to the median diameter of suspension particles in the range of 1 to 4 ${\mu}m$. In brief, the rainfastness of 5 fungicides tested on the pepper leaf was, in the early stage of raining, closely related to water solubility. But, as the raining is progressed, the effect of the unknown factor, which is related with the particle size of fungicides, becomes serious.