• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.1
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• pp.59-64
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• 2017

Scutellaria baicalensis Georgi is one of major herbs used to treat patients with skin diseases diagnosed by wind-heat syndrome. This study was designed to investigate the effects of Scutellaria baicalensis Extract (SBE) on skin lesion of Contact dermatitis (CD) in mice. We investigated the effects of SBE on skin thickness, skin lesions, erythema index and melanin index in vivo. The effects on body weights and spleen/body weight ratio were also investigated in mice with CD induced by topical application of 1-Fluoro-2,4-dinitrofluorobenzene (DNFB). In our results, topical application of SBE lowered skin thickness significantly. In addition, SBE ameliorated skin lesions such as erythema, exudate and petechia in mice with CD. SBE also inhibited erythema index elevated by DNFB significantly. finally, SBE did not affect body weight and spleen/body weight ratio. In conclusion, these data imply that SBE can ameliorate skin lesion of CD such as erythema, exudate and petechia, and be used to treat CD patients with relative safety.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.6
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• pp.455-464
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• 2018

This study investigated whether the whitening effects of fullerene serum occurred through improvement of melanin production and skin tone and evaluated its potential for use as a cosmetic material for adult women aged 30-55 with dull skin color and pigmentation. Subjects were divided into a control group with fullerene-less serum (B) and an experimental group with serum containing 0.1% fullerene (A) and evaluated over 8 weeks. Visual assessment and measurement of skin conditions (melanin index, skin brightness) were conducted before use of and 4 and 8 weeks after use of the product, and a questionnaire survey and safety assessment were conducted at the end of the experiment. The results revealed that the highest decrease was 0.37% in the experimental group (A) after eight weeks of use of the product, but that this difference was not statistically significant. Moreover, no significant differences were observed upon inter-group comparison. Evaluation of changes in the melanin index and inter-group comparison revealed a significant decline in the experimental group (A) at all time points. Additionally, the $L^*$ value (skin brightness) of the experimental group (A) showed a significant increase (improvement) after eight weeks, while in inter-group comparison revealed a significant increase (improvement) at all time points (4 and 8 weeks later) (p<0.05). Based on a questionnaire survey of efficacy, positive response rates such as improvement of the target parts on week 8 were high in terms of 'improvement of skin color.' Moreover, the safety assessment revealed no adverse reactions. In conclusion, serum containing 0.1% fullerene revealed improved melanin production and skin tone (brightness) and confirmed its potential for use as a cosmetic material with brightening efficacy. Taken together, the results of this study indicate systematic investigation of the effective application and cosmetics formulations of this product are warranted.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.4
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• pp.341-348
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• 2023

In this study, basic skin characteristic data was measured by measuring skin hydration, skin sebum secretion rate, skin melanin index, skin redness index, skin redness image analysis, transepidermal water loss (TEWL), and amount of stratum corneum before and after creating a temporary high temperature and low humidity environments targeting Korean women in their 20s to 50s. Stratum corneum by tape stripping was collected at each measurement and skin biomarkers including total protein content, carbonylated protein, neutral lipid, and lipid peroxidation were analyzed. Based on the results, the differences before and after creating a high temperature and low humidity environments were confirmed, the correlation between skin characteristics and skin biomarkers was confirmed, and a new skin index was created based on this. The new skin index can be used in product efficacy evaluation, and the possibility of constructing a new clinical study method and using skin biomarker discovery research through additional research was confirmed.

• Journal of Sasang Constitutional Medicine
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• v.26 no.2
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• pp.180-193
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• 2014

Objectives The present study was carried out to investigate effects of HBPDS on allergic contact dermatitis (ACD) induced by 2,4-Dinitro-1-fluorobenzene (DNFB) in mice. Methods In this experiment, effects of HBPDS on body weights, skin thicknesses, skin weights, histopathological changes, clinical aspects, erythema index, melanin index, production levels of cytokines in ACD mice were investigated. In addition, effects on proliferation rates, release of ${\beta}$-hexosaminidase and histamine were also investigated in vitro. Results & Conclusions 1) HBPDS inhibited enlargement of skin thickness and weight significantly (P < 0.05). 2) HBPDS treatment prevented spongiosis, edema and immune cell infiltrations. 3) Erythema, desquamation and keratosis were diminished by oral administration of HBPDS. 4) Production levels of TNF-alpha and IFN-gamma in serum were decreased by HBPDS treatment in vivo. 5) More than 200 ${\mu}g/ml$ of HBPDS treatment decreased ${\beta}$-hexosaminidase release and more than 400 ${\mu}g/ml$ of HBPDS treatment also decreased histamine release in vitro.

• Journal of Radiation Industry
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• v.10 no.4
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• pp.231-242
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• 2016

This study was conducted to evaluate the synergistic protective effects of mixtures of corn silk, perilla leaf and grape stem extract (CPG mixture) against UVB-induced skin damage and compound 48/80-induced pruritus in mice. The results showed that treatment with CPG mixture exhibited much stronger suppressive effect on erythema and melanin index as well as melanin formation than treatment with ascorbic acid (AA) in UVB-irradiated mice. Moreover, the treatment with CPG mixture showed ameliorative effect on immune cell infiltration and collagen fiber destruction in UV-irradiated mice. The treatment with CPG mixture inhibited glutathione (GSH) depletion, lipid peroxidation and production of pro-inflammatory cytokines in UVB-irradiated mice. Furthermore, the treatment with CPG mixture inhibited compound 48/80-induced scratching behavior and histological changes in mice. Taken together, these results indicated that CPG mixture has potentials as functional and therapeutic materials against skin damage and itch-related skin diseases.

• Journal of Biomedical Engineering Research
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• v.28 no.2
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• pp.195-198
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• 2007

In this paper, we introduce a digital cross-polarization and fluorescent color imaging system for quantitative evaluation of skin lesions. We describe the characterization of the imaging systems and the quantitative image analysis methods to show the feasibility for quantitative evaluation of skin lesions. The polarization color image was used to compute erythema and melanin index image which are useful for quantitative evaluation of pigmentation and vascular skin lesions, respectively. The fluorescent color image was used to quantitatively evaluate "sebum" and "vitiligo". In quantitative evaluation of various skin lesions, we confirmed the clinical efficacy of the imaging systems for dermatological applications. Finally, we sure that the imaging systems can be utilized as important assistant tools for the evaluation of skin lesions by providing reproducible quantitative result for widely distributed skin lesions.

• Applied Microscopy
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• v.38 no.3
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• pp.195-204
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• 2008

This study was intended to identify the effectiveness of Lithospermum erythrorhizon in the UVB-irradiated mouse skin. The C57BL mice were divided into three groups; the control group, the UVB irradiated group(UVB group), and the group treated with Lithospermum erythrorhizon extracts after UVB irradiation(UVB+Le group). 10 mouses were collected and sacrificed at 24 hrs, 48 hrs, 72 hrs, 120 hrs, and 168 hrs, respectively. In the result, the transepidermal water loss (TEWL) was decreased the UVB+Le group than UVB groups by time. At the 168 hrs group was significantly lower(p<0.05). In the result, the melanin value was decreased in the UVB+Le group than UVB group, but meaningless(p>0.05). In the result of erythema index, the UVB+Le group was meaningfully lower at 24 hrs, 48 hrs, and 72 hrs group than UVB group(p<0.05). In the result of scanning electron micrograph observation, the UVB+Le group was allevited swelling than UVB group at the 24 hrs, formation of the scab at the 48 hrs, regular plate shap at the 72 hrs, new keratin observated at the 120 hrs partially, and fine fiber covered epidermis surface at the 168 hrs. In the result of transmission electron micrograph observation, the UVB+Le group was facilitation of increased lamellar bodies and reformation lamellar bodies than UVB group at the all groups. Almost all the structures were recovered at the 160 hrs group. In conclusion, Lithospermum erythrorhizon extracts may recovery on the UVB-irradiated mouse skin.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.1
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• pp.25-32
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• 2022

In this study, basic skin characteristics data were collected by measuring skin hydration, skin melanin, skin redness, and skin torsion elasticity from Korean women in from 20's to 40's ages, and then, age and correlation analysis were conducted. This was used to create a skin index, and cluster analysis was performed to classify the groups into 4 clusters, and the skin characteristics of each cluster were confirmed. Then, two prototypes were used for two weeks to confirm the improvement effect on skin moisture, skin redness, and skin dead mass reduction, and then analyzed which product was more effective in which cluster of subjects participated in the skin characteristics test. As a result of the study, the possibility of preparing for the customized cosmetics market was confirmed by applying the skin index and cluster analysis results to product efficacy evaluation.

• Korean Journal of Acupuncture
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• v.28 no.3
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• pp.63-71
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• 2011

Objectives : The aim of this study is to develop the system and evaluate the optical analytical technique that reflects acupoint pigmentation and extravasated blood by cupping. Methods : We designed the system able to express XYZ coordinate on local skin color. To evaluate measurement-accuracy, we compared with 24 Color Checker Chart by standardized Commission Internationale de l'Eclairage. After confirming the performance of system, we experimented with cupping which was 80 kPa negative pressure for 1 minute on left/right BL13. The X, Y and Z values were converted to R, G and B, $L^*$, $a^*$ and $b^*$, Erythema Index (E.I.), and Melanin Index (M.I). We compared and analyzed two cases on before/after cupping. Results : The R, G and B values which were converted by X, Y and Z values had high linearity as a high level of R-square (R: 0.969, G: 0.996, and B: 0.992). Moreover, we confirmed that it was possible to quantitatively analyzed the change in skin color by cupping using R, G, B, $L^*$, $a^*$, $b^*$, E.I., and M.I. Conclusions : Therefore, we proposed the new analytical technique for objectifying the oriental medical diagnostic method using cupping and optical sensing technique.

• Herbal Formula Science
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• v.25 no.3
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• pp.337-347
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• 2017

Objectives : Atopic Dermatitis (AD) is chronic skin disease characterized by allergic hypersensitivity reactions. Samhwangsasim-tang Gamibang(SHSST) can treat skin disease by cooling down blood heat, clearing away congenital fever and detoxicating. This study was conducted to investigate the effects of SHSST on AD induced by oxazolone in mice. Methods : We investigated the effects of SHSST on skin tickness, skin lesions, erythema and melanin index, water content and histopathological changes in vivo. The effects on body weights and spleen body weight ratio were also investigated in mice with AD. Results : In our results, oral administration of SHSST ameliorated skin lesion such as flare, erythema, exudate and petechia and lowered skin thickness induced by oxazolone. In addition, SHSST lowered erythema index and elevated water content compared to AD control respectively. In the histopathological observation, oral administration of SHSST prevented epidermal hyperplasia and spongiotic changes which is a hallmark of skin inflammation respectively. Finally, SHSST did not affect spleen/body weight ratio. Conclusions : These data imply that SHSST can ameliorate skin lesion of AD such as flare, erythema, exudate and petechia, and be used to treat AD patients with relative safety.