Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Effects of Hyeongbangpaedok-san (HBPDS) on Allergic Contact Dermatitis (ACD) induced by DNFB in Mice

형방패독산(荊防敗毒散)이 DNFB로 유발된 생쥐의 알레르기접촉피부염에 미치는 영향

  • Park, Geun-Hee (Dept. of Sasang Contitutional Medicine, Graduate School of Dongshin University) ;
  • Lim, Tae-Hyung (Dept. of Sasang Contitutional Medicine, Graduate School of Dongshin University) ;
  • Park, Hye-Sun (Dept. of Sasang Contitutional Medicine, Graduate School of Dongshin University)
  • 박근희 (동신대학교 한의과대학 사상체질의학과) ;
  • 임태형 (동신대학교 한의과대학 사상체질의학과) ;
  • 박혜선 (동신대학교 한의과대학 사상체질의학과)
  • Received : 2014.05.07
  • Accepted : 2014.06.23
  • Published : 2014.06.30
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Abstract

Objectives The present study was carried out to investigate effects of HBPDS on allergic contact dermatitis (ACD) induced by 2,4-Dinitro-1-fluorobenzene (DNFB) in mice. Methods In this experiment, effects of HBPDS on body weights, skin thicknesses, skin weights, histopathological changes, clinical aspects, erythema index, melanin index, production levels of cytokines in ACD mice were investigated. In addition, effects on proliferation rates, release of ${\beta}$-hexosaminidase and histamine were also investigated in vitro. Results & Conclusions 1) HBPDS inhibited enlargement of skin thickness and weight significantly (P < 0.05). 2) HBPDS treatment prevented spongiosis, edema and immune cell infiltrations. 3) Erythema, desquamation and keratosis were diminished by oral administration of HBPDS. 4) Production levels of TNF-alpha and IFN-gamma in serum were decreased by HBPDS treatment in vivo. 5) More than 200 ${\mu}g/ml$ of HBPDS treatment decreased ${\beta}$-hexosaminidase release and more than 400 ${\mu}g/ml$ of HBPDS treatment also decreased histamine release in vitro.

Keywords

References

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