• Microbiology and Biotechnology Letters
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• v.35 no.1
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• pp.11-16
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• 2007

The arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora genome was amplified by PCR and subcloned into the pHCE-IA vector, in which the hyper consitutive expression (HCE) promoter from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toevii was employed. The transformant cell, Escherichia coli BL21 (DE3)/pHCE-AST, on LB agar plate containig 4-methylumbelliferyl sulfate, showed an intense fluorescence at 360 nm, indicating that 4-methylumbelliferone was liberated by desulfatate activity. When BL21 (DE3)/pHCE-AST was grown on LB media containing 0.4% glucose or 0.4% glycerol, the arylsulfatase activity was higher at glycerol rather than at glucose. On 2% glycerol medium, the arylsulfatase activity reached 15.0 unit/ml, which was 2.6-fold higher expression level than that with 1% glycerol. The DNA ladder in agarose prepared from agar by this recombinant enzyme revealed similar resolution and migration patterns with a commercial agarose. This results suggests that arylsulfatase overexpressed in E. coli could be applicable to the economic production of electrophoretic-grade agarose.

• Microbiology and Biotechnology Letters
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• v.37 no.4
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• pp.355-360
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• 2009

In this study, the arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora genome was expressed on the cell surface of S. cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The constructed plasmid, pCTAST (7.1 kb), was introduced to S. cerevisiae EBY100 cell, and yeast transformants on YPDG plate showed the hydrolyzing activity for 4-methylumbelliferyl-sulfate and p-nitrophenyl-sulfate. When S. cerevisiae EBY100/pCTAST was grown on YPDG medium, the arylsulfatase activity of cell pellet reached about 1.2 unit/mL, whereas no extracellular arylsulfatase activity was detected. The DNA ladder in agarose prepared from agar by this recombinant arylsulfatase showed similar resolution and migration patterns with a commercial agarose. This results revealed that arylsulfatase expressed on the cell surface of S. cerevisiae could be applicable to the economic production of electrophoretic-grade agarose.

• Proceedings of the KSRS Conference
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• v.2
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• pp.827-830
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• 2006

The tsunami from the megathrust earthquake magnitude 9.3 on 26 December 2004 is the largest tsunami the world has known in over forty years. This tsunami destructively attacked 13 countries around Indian Ocean with at least 230,000 fatalities, displaced people 2,089,883 and 1.5 million people who lost their livelihoods. The ratio of women and children killed to men is 3 to 1. The total damage costs US$ 10.73 billion and rebuilding costs US$ 10.375 billion. The tsunami's death toll could have been drastically reduced, if the warning was disseminated quickly and effectively to the coastal dwellers along the Indian Ocean rim. With a warning system in Indian Ocean similar to that operating in the Pacific Ocean since 1965, it would have been possible to warn, evacuate and save countless lives. The best tribute we can pay to all who perished or suffered in this disaster is to heed its powerful lessons. UNESCO/IOC have put their tremendous effort on better disaster preparedness, functional early warning systems and realistic arrangements to cope with tsunami disaster. They organized ICG/IOTWS (Indian Ocean Tsunami Warning System) and the third of this meeting is held in Bali, Indonesia during $31^{st}$ July to $4^{th}$ August 2006. A US$ 53 million interim warning system using tidal gauges and undersea sensors is nearing completion in the Indian Ocean with the assistance from IOC. The tsunami warning depends strictly on an early detection of a tsunami (wave) perturbation in the ocean itself. It does not and cannot depend on seismological information alone. In the case of 26 December 2004 tsunami when the NOAA/PMEL DART (Deep-ocean Assessment and Reporting of Tsunami) system has not been deployed, the initialized input of sea surface perturbation for the MOST (Method Of Splitting Tsunami) model was from the tsunamigenic-earthquake source model. It is the first time that the satellite altimeters can detect the signal of tsunami wave in the Bay of Bengal and was used to validate the output from the MOST model in the deep ocean. In the case of Thailand, the inundation part of the MOST model was run from Sumatra 2004 for inundation mapping purposes. The medium and high resolution satellite data were used to assess the degree of the damage from Indian Ocean tsunami of 2004 with NDVI classification at 6 provinces on the Andaman seacoast of Thailand. With the tide-gauge station data, run-up surveys, bathymetry and coastal topography data and land-use classification from satellite imageries, we can use these information for coastal zone management on evacuation plan and construction code.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.1
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• pp.47.1-47.1
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• 2018

The NISS (Near-infrared Imaging Spectrometer for Star formation history) onboard NEXTSat-1 have successfully developed by KASI. The capability of both imaging and spectroscopy is a unique function of the NISS. At first, it have realized the low-resolution spectroscopy (R~20) with a wide field of view of $2{\times}2deg$. in a wide near-infrared range from 0.95 to $2.5{\mu}m$. The major scientific mission is to study the cosmic star formation history in local and distant universe. It will also demonstrate the space technologies related to the infrared spectro-photometry in space. Now, the NISS is ready to launch in late 2018. After the launch, the NISS will be operated during 2 years. As an extension of the NISS, the SPEHREx (Spectro-Photometer for the History of the Universe Epoch of Reionization, and Ices Explorer) is the NASA MIDEX (Medium-class Explorer) mission proposed together with KASI (PI Institute: Caltech). It will perform the first all-sky infrared spectro-photometric survey to probe the origin of our Universe, to explore the origin and evolution of galaxies, and to explore whether planets around other stars could harbor life. Compared to the NISS, the SPHEREx is designed to have much more wide FoV of $3.5{\times}11.3deg$. as well as wide spectral range from 0.75 to $5.0{\mu}m$. After passing the first selection process, the SPHEREx is under the Phase-A study. The final selection will be made in the end of 2018. Here, we report the status of the NISS and SPHEREx missions.

• Journal of Korean Society of Forest Science
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• v.101 no.3
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• pp.365-373
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• 2012

This study purposed to extract and assess the accuracy of assessment for deforestation area in Wonju city using medium resolution satellite image. The total size of deforestation area during the last nine years (2000-2008) was about 467 ha, and it was occurred annually about 52 ha. The most frequent form of deforestation was settlements (72%). Ninety percent of the size of deforestation was less than 2 ha in size. In addition, 79 percent of deforestation area was found within 500 m from the road network and within 100 m of the Forest/Non-forest boundary. This study compared the deforestation based on the administrative information (GIS deforestationI) with the deforestation (RS deforestation) extracted from the satellite imagery by vegetation indices (NDVI, NBR, NDWI). Extraction accuracy, mean-standard deviation${\times}1.5$ applied 3 by 3 filtering, showed reliable accuracy 35.47% k-value 0.20. However, error could be occurred because of the difference of land-use change and land-cover change. The actual rate of land-cover change deforestation area was 32% on administrative information. The 7.52% of forest management activities area was misjudged as deforestation by RS deforestation. Finally, the comparison of land-cover change deforestation (GIS deforestationII) with the RS deforestation accuracy, as a result NDVI mean-standard deviation${\times}2$ applied 3 by 3 filtering, showed improved accuracy 61.23%, k-value 0.23.

• Journal of Korean Society for Geospatial Information Science
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• v.18 no.1
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• pp.135-143
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• 2010

The national base map has its value sand roles as the basic spatial information of the nation. The current national base map that is a 1/5,000 digital map, however, has failed to perform its roles as basic spatial information due to the limitations with its quality and accuracy and requires measures to complement them. Thus this study set out to suggest ways to advance the current 1/5,000 national base map, selected topography and natural features of a digital map that could be made with GSD 0.25m digital aerial photographs, and set up the optimal ways to make a digital map by conducting an experiment of making an optimal digital map with such photographs. It also analyzed the map made with GSD 0.25m digital aerial photographs for accuracy and usability. In order to establish a set of criteria of making a digital map with GSD 0.25m digital aerial photographs, the investigator carried out analyses and picked topography and natural features items, which include 9 large categories, 31 medium categories, and 509 small categories. Then an experiment of making a digital map was conducted according to the digital map making method. As a result, solid drawing was selected as the optimal way to making a digital map, and the optimal process was established. Using the research achievements, a model digital map was made with GSD 0.25mm digital aerial photographs. The map recorded about two times horizontal and vertical location accuracy than the old 1/5,000 digital map and was capable of detailed descriptions of topography and natural features. A new national base map made with GSD 0.25m digital aerial photographs will provide reliable spatial data, thus increasing the level of satisfaction among people and the level of advancement of national base maps.

• Korean Journal of Remote Sensing
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• v.32 no.6
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• pp.579-587
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• 2016

To obtain a stable energy supply and manage PhotoVoltaic (PV) systems efficiently, satellite imagery methods are being developed to estimate the solar PV potential. This study analyzed trends in the use of satellite imagery in solar PV and solar irradiation estimation technology. The imaging technology is used to produce solar energy resource maps. The trend analysis showed that the level of solar PV technology in Korea is 30% below that of advanced countries. It is impossible to raise such low-level technologies to the levels of advanced countries quickly. Intensive research and development is the only way to achieve the 80% technology level of advanced countries. The information produced in this process can contribute to the management of solar power plants. A valid technology development strategy would be to obtain effective data that can be used for fieldwork. Such data can be produced by estimating solar irradiation very accurately with several-hundred-meter resolution using Communication, Ocean, and Meteorological Satellites (COMS) and next-generation GEO-KOMPSAT 2A, developing core technologies for short- and medium-term irradiation prediction, and developing technologies for estimating the solar PV potential.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.11
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• pp.3121-3129
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• 2009

Conventional marine radar systems utilize pulse radar which is capable of high-power transmissions and is effective for remote detection purposes. A pulse radar is most commonly used on medium or large vessels due to its expensive installation and maintenance costs. I propose the use of a Frequency Modulated Continuous Wave (FMCW) radar system operated at low-power and high-resolution instead of the conventional pulse-radar based system. The transmitted and received signals of the FMCW radar system were theoretically analyzed and radar signal processing design and simulation experiments were performed to detect the range and speed. Intermediate Frequency (IF) signal mixed with virtual transmit and receive signals were generated to perform FMCW radar signal processing simulations where the IF signal underwent noise reduction through a lowpass filter. The maximum frequency was derived through the sample interval of the FFT size instead of using A/D converter. This maximum frequency was used to get the frequency range and frequency speed which were in turn used to calculate the range and speed. The virtual beat frequency generated using MATLAB is utilized to analyze the beat frequency used in the actual FMCW radar system signal processing. The differences in the range and speed of the beat frequency signals are processed and analyzed.

• Korean Journal of Optics and Photonics
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• v.28 no.4
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• pp.158-165
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• 2017

The physical shape of an ultrasound transducer has not been considered in previous studies of the photoacoustic saturation effect, where a photoacoustic signal's magnitude linearly increases as an absorption coefficient increases and it is finally saturated. In this paper, the effect of a spherically focused ultrasound transducer on photoacoustic nonlinearity is investigated. The focused ultrasound transducer's spatial filtering effect on photoacoustic signals is analytically derived considering the combined concept of a virtual point detector and Green function approach. The ultrasound transducer's temporal response (i.e., transfer function) effect on photoacoustic signals is considered by integrating photoacoustic signal values within the absorption area covered by a spatial resolution of the ultrasound transducer. Results from the analytically derived expression show that the magnitude of photoacoustic signals measured by a spherical focused ultrasound transducer shows a maximum at a specific absorption coefficient, and decreases after that maximum point as an absorption coefficient is increased. The origin of this photoacoustic nonlinearity is physically understood by comparing the ultrasound transducer's transfer functions and photoacoustic resonance spectra. In addition, this physical interpretation implies that the photoacoustic nonlinearity is strongly dependent on the irradiance distribution inside an absorption medium.

• Journal of Microbiology and Biotechnology
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• v.27 no.7
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• pp.1249-1256
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• 2017

In our search for new sources of bioactive secondary metabolites from Streptomyces sp., the ethyl acetate extracts from endophytic Streptomyces SUK 25 afforded five active diketopiperazine (DKP) compounds. The aim of this study was to characterize the bioactive compounds isolated from endophytic Streptomyces SUK 25 and evaluate their bioactivity against multiple drug resistance (MDR) bacteria such as Enterococcus raffinosus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter spp., and their cytotoxic activities against the human hepatoma (HepaRG) cell line. The production of secondary metabolites by this strain was optimized through Thornton's medium. Isolation, purification, and identification of the bioactive compounds were carried out using high-performance liquid chromatography, high-resolution mass liquid chromatography-mass spectrometry, Fourier transform infrared spectroscopy, and nuclear magnetic resonance, and cryopreserved HepaRG cells were selected to test the cytotoxicity. The results showed that endophytic Streptomyces SUK 25 produces four active DKP compounds and an acetamide derivative, which were elucidated as $cyclo-({\text\tiny{L}}-Val-{\text\tiny{L}}-Pro)$, $cyclo-({\text\tiny{L}}-Leu-{\text\tiny{L}}-Pro)$, $cyclo-({\text\tiny{L}}-Phe-{\text\tiny{L}}-Pro)$, $cyclo-({\text\tiny{L}}-Val-{\text\tiny{L}}-Phe)$, and N-(7-hydroxy-6-methyl-octyl)-acetamide. These active compounds exhibited activity against methicillin-resistant S. aureus ATCC 43300 and Enterococcus raffinosus, with low toxicity against human hepatoma HepaRG cells. Endophytic Streptomyces SUK 25 has the ability to produce DKP derivatives biologically active against some MDR bacteria with relatively low toxicity against HepaRG cells line.