• KSBB Journal
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• v.6 no.4
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• pp.385-388
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• 1991

Embryogenic cell suspension cultures of B. striata were established as transferred selected embryogenic callus into liquid medium. Protoplasts isolated from embryogenic cell suspensions were electroporated in buffered solutions containing plasmid DNA of pBI121. Transient GUS (beta-glucuronidase) activity measurement and selection for kanamycin resistent showed that expression of foreign genes and stable transformation were achieved. GUS transient gene expression was increased by increasing DNA concentration of pBI121 plasmid and affected by the level of the applied voltage. An optimal level of GUS activity was obtained after electroporation with a pulse of 200-300 voltage/1180 uF. Protoplast viability was up to the 80% at the optimal voltage.

• Structural Engineering and Mechanics
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• v.76 no.5
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• pp.619-629
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• 2020

In current study, surface/interface effects for pull-in voltage and viscous fluid velocity effects on dimensionless natural frequency (DNF) of fluid-conveying piezoelectric nanosensor (FCPENS) subjected to direct electrostatic voltage DC with nonlinear excitation, harmonic force and also viscoelastic foundation (visco-pasternak medium and structural damping) are investigated using Gurtin-Murdoch surface/interface (GMSIT) theory. For this analysis, Hamilton's principles, the assumed mode method combined with Lagrange-Euler's are used for the governing equations and boundary conditions. The effects of surface/interface parameters of FCPENS such as Lame's constants (λI,S, μI,S), residual stress (τ0I,S), piezoelectric constants (e31psk,e32psk) and mass density (ρI,S) are considered for analysis of dimensionless natural frequency respect to viscous fluid velocity u̅f and pull-in voltage V̅DC.

• Journal of the Korean Vacuum Society
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• v.8 no.3B
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• pp.346-352
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• 1999

Diamond-like carbon (DLC) lims were deposited by using end hall type ion gun. Benzene gas was used for the generation of carbon ions. In order to systematically control the ion energy, we applied to the substrate DC, pulsed DC or 250 kHz medium frequency bias voltage, DLC films of superior mechanical properties of hardness 39$\pm$4 GPa and elastic mudulus 290$\pm$50GPa (2 to 6 times better than those of the films deposited by plasma assisted CVD method) could be obtained. Deposition rate was much higher than when using Kaufman type ion source, which results from higher ion beam current of end hall type ion gun. The mechanical properties and atomic bond structure were independent of the bias voltage type ion gun. The mechanical properties and atomic bond structure were independent of the bias voltage type but intimately related with the magnitude of the bias voltage. With increasing the negative bias voltage, the structure of the films changed to graphitic one resulting in decreased content of three dimensional inter-links. Degradation of the mechanical properties with increasing bias voltage could be thus understood in terms of the content odf three dimensional inter-links.

• Journal of Power Electronics
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• v.9 no.2
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• pp.133-145
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• 2009

In this paper a modular high performance MV-to-LV rectifier based on a cascaded H-bridge rectifier is presented. The proposed rectifier can directly connect to the medium voltage levels and provide a low-voltage and highly-stable DC interface with the consumer applications. The input stage eliminates the necessity for heavy and bulky step-down transformers. It corrects the input power factor and maintains the voltage balance among the individual DC buses. The second stage includes the high frequency parallel-output DC/DC converters which prepares the galvanic isolation, regulates the output voltage, and attenuates the low frequency voltage ripple ($2f_{line}$) generated by the first stage. The parallel-output converters can work in interleaving mode and the active load-current sharing technique is utilized to balance the load power among them. The detailed analysis for modeling and control of the proposed structure is presented. The validity and performance of the proposed topology is verified by simulation and experimental results.

• Journal of the Korean Chemical Society
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• v.18 no.2
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• pp.110-116
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• 1974

Reduction of chromate ion at the Pt-electrode was investigated in neutral unbuffered solutions, in buffered solutions of pH between 8 and 10, and in strongly alkaline medium. In buffered solutions of pH between 8 and 10, the number of electrons transfered in the reduction of chromate ion increased progressively with increasing pH. When chromate ion was reduced in 0.2 N sodium hydroxide medium the following mechanism was suggested: $CrO_4^=+H_2O+2e{\rightarrow}CrO_3^=+2OH^-,\;CrO_3^=3H_2O+e{\rightarrow}Cr(OH)_3+3OH^-$ When tetramethylammonium hydroxide (pH=13.5) was used as the supporting electrolyte, a second wave indicated strong adsorption. In unbuffered solutions of 0.1 N potassium chloride the linear sweep voltammogram consists of three or four distinct waves depending on the initial voltage and the voltage sweep rates, but the first wave was difficult to explain as a diffusion controlled wave.

• Development and Reproduction
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• v.24 no.4
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• pp.297-306
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• 2020

Repetitive changes in the intracellular calcium concentration ([Ca²⁺]i) triggers egg activation, including cortical granule exocytosis, resumption of second meiosis, block to polyspermy, and initiating embryonic development. [Ca²⁺]i oscillations that continue for several hours, are required for the early events of egg activation and possibly connected to further development to the blastocyst stage. The sources of Ca²⁺ ion elevation during [Ca²⁺]i oscillations are Ca²⁺ release from endoplasmic reticulum through inositol 1,4,5 tri-phosphate receptor and Ca²⁺ ion influx through Ca²⁺ channel on the plasma membrane. Ca²⁺ channels have been characterized into voltage-dependent Ca²⁺ channels (VDCCs), ligand-gated Ca²⁺ channel, and leak-channel. VDCCs expressed on muscle cell or neuron is specified into L, T, N, P, Q, and R type VDCs by their activation threshold or their sensitivity to peptide toxins isolated from cone snails and spiders. The present study was aimed to investigate the localization pattern of N and P/Q type voltage-dependent calcium channels in mouse eggs and the role in fertilization. [Ca²⁺]i oscillation was observed in a Ca²⁺ contained medium with sperm factor or adenophostin A injection but disappeared in Ca²⁺ free medium. Ca²⁺ influx was decreased by Lat A. N-VDCC specific inhibitor, ω-Conotoxin CVIIA induced abnormal [Ca²⁺]i oscillation profiles in SrCl₂ treatment. N or P/Q type VDC were distributed on the plasma membrane in cortical cluster form, not in the cytoplasm. Ca²⁺ influx is essential for [Ca²⁺]i oscillation during mammalian fertilization. This Ca²⁺ influx might be controlled through the N or P/Q type VDCCs. Abnormal VDCCs expression of eggs could be tested in fertilization failure or low fertilization eggs in subfertility women.

• Proceedings of the KIPE Conference
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• 2004.11a
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• pp.1-4
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• 2004

From standstill to medium speed which enough to sensing back EMF, it is not easy to drive without additional equipments due to directly proportional to flux. Conventional method to drive is 'align and go' method, so called v/f control that is increased voltage duty in proportion to the increase of frequency. Because it isn't consider load of motor and doesn't know rotor position exactly, current is supplied too much current or not enough to rotate motor. This paper is proposed starting method without any poison sensor and can drive a BLDC motor from standstill to the medium speed smoothly without any rattling and time delay compared with the conventional starting algorithm. To verify algorithm of this paper, experimental results are presented.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1999.11a
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• pp.358-361
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• 1999

This paper describes on the fabrication and characteristics of hot-film type micro-flowsensors integrated with Pt-RTD\`s and micro-heater on the Si substrate, in which MgO thin-films were used as medium layer in order to improve adhesion of Pt thin-films to SiO$_2$ layer The MgO layer improved adhesion of Pt thin-films to SiO$_2$` layer without any chemical reactions to Pt thin-films under high as gas flow rate and its conductivity increased due to increase of heat-loss from sensor to external. Output voltage was 82 mV at N2 flow rate of 2000 sccm/min, heating power of 1.2W. The respons time was about 100 msec when input flow was step-input

• Journal of Electrical Engineering and Technology
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• v.11 no.6
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• pp.1656-1663
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• 2016

A capacitive wireless power transfer (C-WPT) system uses an electric field to transmit power through a physical isolation barrier which forms a pair of ac link capacitors between the metal plates. However, the physical dimension and low dielectric constant of the interface medium severely limit the effective link capacitance to a level comparable to the main switch output capacitance of the transmitting circuit, which thus narrows the soft-switching range in the light load condition. Moreover, by fundamental limit analysis, it can be proved that such a low link capacitance increases operating frequency and capacitor voltage stress in the full load condition. In order to handle these problems, this paper investigates optimal design of double matching transformer networks for C-WPT. Using mathematical analysis with fundamental harmonic approximation, a design guideline is presented to avoid unnecessarily high frequency operation, to suppress the voltage stress on the link capacitors, and to achieve wide ZVS range even with low link capacitance. Simulation and hardware implementation are performed on a 5-W prototype system equipped with a 256-pF link capacitance and a 200-pF switch output capacitance. Results show that the proposed scheme ensures zero-voltage-switching from full load to 10% load, and the switching frequency and the link capacitor voltage stress are kept below 250 kHz and 452 V, respectively, in the full load condition.

• Journal of Power Electronics
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• v.16 no.2
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• pp.455-463
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• 2016

This paper proposes a compensation method to improve the distorted space vectors when a 3-level Neutral Point Clamped (NPC) inverter has an unbalanced neutral point voltage. Since both the neutral point voltage of the DC link and the space vector of a 3-level NPC inverter are closely related depending on the output load connecting state, a distorted space vector can occur when the neutral point voltage of a 3-level NPC inverter is unbalanced. The proposed method can improve the distorted space vectors by adjusting the injection time of the small and medium vectors and by modulating the amplitude of the carrier waveforms. In this paper, the proposed method is verified by both simulation and experimental results based on a 3-level NPC inverter.