• Journal of Plant Biotechnology
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• 제46권3호
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• pp.143-157
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• 2019

Medicinal plants are high-value natural resources that have been used as precautionary drugs by many people globally. The increasing global demand for bioactive compounds from medicinal plants has led to the overexploitation of many valuable species. One widely used approach to overcome this problem is the use of adventitious root cultures as a propagation strategy. This review examines the scientific research published globally on the application of adventitious root cultures for many medicinal plants. Adventitious roots generated under aseptic environments in suitable phytohormone-augmented medium exhibit high growth rates and production of important secondary metabolites. Parameters such as medium properties and composition, growth hormone type, and elicitation strategies for in vitro grown adventitious roots of medicinal plants, are the main topics discussed in this review. We also examine current developments in bioreactor system cultivation for plant bioactive compounds using adventitious root cultures, a technology with possible commercial applications, via several studies on adventitious root culture of medicinal plants in which bioreactor systems play a role. In conclusion, the development of adventitious root cultures for medicinal plants is highly useful because of their capability for vegetative propagation and germplasm preservation.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.1028-1034
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• 2021

The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated. Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation. To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid. Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solid-state fermentation. Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation. Therefore, solid-state fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.

• 한국수처리학회지
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• 제26권6호
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• pp.27-42
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• 2018

This study analyzed the utility of ammonium chloride ($NH_4Cl$) as a nitrogen source for methanotroph communities. When cultured in nitrate mineral salt (NMS) medium, the methanotroph community we identified four families, seven genera, and 16 type I and type II species of methanotrophs. Among species in the Methylobacter genus, Methylobacter marinus could be actively cultured in NMS medium without NaCl addition. Following the addition of 25 mM $NH_4Cl$, the numbers of the type I genera Methylomonas, Methylococcus, and Methylobacter were increased, whereas the numbers of the type II genera Methylocystis and Methylosinus were decreased after 5 days. In methanotroph communities, certain concentrations of $NH_4Cl$ affected methane consumption and growth of methanotrophs at the community level. $NH_4Cl$ caused a considerable decrease in the methane consumption rate and the expression of soluble methane monooxygenases (sMMOs) but did not inhibit the growth of Methylomonas methanica expressing sMMO. These results could be attributed to competitive antagonism of MMOs due to their direct involvement in ammonia oxidation.

• 한국의류학회지
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• 제45권1호
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• pp.200-216
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• 2021

This article considers short-form fashion videos as distinguished from fashion films, defines the concept, details the expressive characteristics of short-form fashion video, and reveals the method of producing it. For the methodology, a literature review was conducted to derive the concept and expression techniques. A case study was also performed to define the expressive characteristics. Five short-form fashion videos were also produced based on the results. The final results are as follows. First, short-form fashion video was defined as a fashion medium on the purpose of fashion communication within 60 seconds and classified by three digital image formats. Second, the result of analyzing the expression of the short-form fashion video shows the simplicity and reconstitution, characterization and remediation, borderless and expansion, and synesthesia trigger of the fashion image. Third, five short-form fashion videos were produced based on the theme of the digital garden. It shows that the short-form fashion video intensively expresses the content as a medium whose sensational expression is more prominent than the composition of the story by the short running time that reflects the taste of digital mainstream.

• 영어영문학
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• 제58권6호
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• pp.1167-1191
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• 2012

For Romantic women writers the paratext itself is essentially a masculine literary space affiliated with established writing practices; however, this paper suggests that Charlotte Turner Smith's mode of discourse in her use of notes and their relation to the text proper are never fixed in her contemplative blank-verse long poem, Beachy Head (1807). Even though the display of learning in the paratext partly supports the woman writer's claim to authority, this paper argues that Smith's endnotes also indicate her way of challenging the double bind for women writers, summoning masculine authority on the margins of her book while simultaneously interrogating essentialist thinking and instructions about one's identity in a culture and on the printed page. The poem shows how the fringes of the book can be effectively transformed from a masculine site of authority to an increasingly feminized site of interchange as Smith writes with an awareness of patriarchal, imperial abuses of power in that area of the book. There is a persistent transgression of cultural/textual boundaries occurring in Beachy Head, which explores the very scene and languages of imperial encounter. Accordingly, if Wordsworth's theory of composition suggests a subjective and abstract poetic experience-an experience without mediation-in which its medium's purpose seems to be to disappear from the reader's consciousness, an examination of the alternative discourse of self-exposure in Smith's poem reveals the essentially fluid nature of media-consciousness in the Romantic era, which remains little acknowledged in received accounts of Romantic literary culture.

• 한국재료학회지
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• 제34권5호
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• pp.247-253
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• 2024

The separation of zirconium and hafnium using tributyl phosphate (TBP)-Dodecane extractants in nitric acid medium was performed. Zirconium oxychloride, used as extraction feed, was obtained from the synthesis of Kalimantan zircon sand concentrate smelted using NaOH. The extraction process was carried out by dissolving chloride-based metals in nitric acid media in the presence of sodium nitrate using TBP-Dodecane as an extractant. Some of the extraction parameters carried out in this study include variations in organic phase and aqueous phase (O/A), variations in contact time, and variations in nitric acid concentration. Extraction was carried out using a mechanical shaker according to the parameter conditions. X-ray fluorescence (XRF) was used for elemental (Zr and Hf) composition analysis of the aqueous solution. The results showed that zirconium was separated from hafnium at optimum conditions with an organic/aqueous ratio of 1:5, contact time of 75 min, and an HNO₃ concentration of 7 M. The resulting separation factor of zirconium and hafnium using TBP-Dodecane was 14.4887.

• Journal of Photoscience
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• 제9권2호
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• pp.341-343
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• 2002

Bacteriochlorophyll(BChl)-c containing species of green sulfur photosynthetic bacterium Chlorobium (ChI.) vibrioforme, which has BChl-d mainly, was detected. We obtained colonies on agar plates by spreading the liquid culture of ChI. vibrioforme f. sp. thiosulfatophilum strain NCIB 8327 which contained the high ratio of BChl-c/BChl-d, and transferred each colony into a new liquid medium. These cultures after growing were found to be classified into two categories. One possessed BChl-d as a light-harvesting pigment and the other did BChl-c. No colonies examined here contained both BChls-d and c. Therefore, the presence of both BChls-d and c in our cultures of ChI. vibrioforme was ascribed to the coexistence of two different cells which had BChl-d and c as the chlorosomal pigment, respectively. The change of pigment composition observed in our liquid cultures can be thus explained by the difference of growth rates between two kinds of cells.

• 한국자기학회지
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• 제11권6호
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• pp.278-284
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• 2001

최근 특허의 권리는 각 나라의 경쟁력에 지대한 영향을 미치고 있다. 특히 첨단기술 분야에 원천기술을 갖고 있다면 그 나라의 기술경쟁력은 우위를 차지하고 있다고 말할 수 있다 앞으로 우리 나라의 연구소나 대학의 연구결과가 특허 출원과 연관되어야, 정부출연 연구비의 투자가 국가 기술력을 증진시킬 것이다 자기기록매체분야는 자성의 특성을 조절하기 위해 합금매체를 사용하게 되는데, 이와 같은 합금은 특허출원명세서 작성 시 주의해야한다. 특히 동일성을 회피하기 위한 청구범위의 작성에 유념해야 한다. 즉, 합금발명의 특허성은 (1)합금성분의 조성범위 및 필요에 따른 조직상태 (2) 그 합금만이 가지고 있는 고유한 성질 또는/및 용도의 각 사항에 의해서 판단하므로 합금발명의 성립 내지 완성은 기계·기구 등 타 분야에 비해 엄격한 기준이 적용되고 있음을 주의할 필요가 있다.

• 한국식품영양과학회지
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• 제24권5호
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• pp.771-778
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• 1995

이 연구는 Bacillus cereus IAM1072의 배양액에서 직접 올리고당을 생산하기 위해 행하였다. 올리고당 생성 배양조건은 접종비 3%, pH, 7.0, 온도 $30^{\circ}C$, 배지액량 40ml, 배양시간 24시간이었으며, 올리고당 생성에 가장 효과적인 최적 배지조성은 12% soluble starch, 1.5% perptone, 0.25% $K_2HPO_4$이었다. 생성된 올리고당 중 $G_5$ 올리고당을 정제하였다. Methylation을 한 후 GC 분석을 한 결과 $G_5$ 올리고당은 glucose 단위가 5개로 구성된 ${\alpha}-1,4-glucosidic$ 결합한 올리고당이었다.

• 대한의생명과학회지
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• 제10권3호
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• pp.293-298
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• 2004

This study was performed to compare the growth rates, cellular fatty acid compositions and morphology by using electron microscope of Escherichia coli (E. coli) grown in various conditions including different concentrations of phenol, CdCl₂ and HgCl₂. Ninety eight E. coli strains were isolated from Naktong river and human feces. The content of unsaturated fatty acids, especially 16:1ω7c and 18:1ωc increased as the concentration of phenol and CdCl₂ increased. The content of unsaturated fatty acid increased up to 50 ppb of HgCl₂, but decreased at 75 ppb of HgCl₂. There were more unsaturated fatty acids than saturated fatty acid in the presence of toxic substances. However, the ration was reversed when the affected E. coli was transferred to toxic substance free fresh trypticase soy broth medium. Also, by using transmission electron microscope these cells were observed to various morphological deformation by heavy metals and their deposition on the surface. From these results, we suggested that the changes of major fatty acids composition and morphology of E. coli may be considered to indicate contaminated levels of heavy metals or organic solvents. The information presented here may be useful in predicting effects of heavy-metal and organic solvent contamination in streams and provides a basis for further studies of metal or organic solvent effects on microbial communities.