Journal of Korean Society of Water Science and Technology (한국수처리학회지)

Korean Society of Water Science and Technology (한국수처리학회)
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Effect of Ammonium Chloride on the Mixed Methanotrophs Species Composition and Methanol Metabolism

염화암모늄 영향에 따른 혼합종 메탄산화균의 종조성 변화 및 메탄올 대사 특성

  • Kim, I Tae (Department of Land, Water and Environment Research, Korea Institute of Civil Engineering and Building Technology) ;
  • Yoon, Younghan (Department of Land, Water and Environment Research, Korea Institute of Civil Engineering and Building Technology)
  • 김이태 (한국건설기술연구원 국토보전연구본부) ;
  • 윤영한 (한국건설기술연구원 국토보전연구본부)
  • Received : 2018.10.08
  • Accepted : 2018.11.20
  • Published : 2018.12.31
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Abstract

This study analyzed the utility of ammonium chloride ($NH_4Cl$) as a nitrogen source for methanotroph communities. When cultured in nitrate mineral salt (NMS) medium, the methanotroph community we identified four families, seven genera, and 16 type I and type II species of methanotrophs. Among species in the Methylobacter genus, Methylobacter marinus could be actively cultured in NMS medium without NaCl addition. Following the addition of 25 mM $NH_4Cl$, the numbers of the type I genera Methylomonas, Methylococcus, and Methylobacter were increased, whereas the numbers of the type II genera Methylocystis and Methylosinus were decreased after 5 days. In methanotroph communities, certain concentrations of $NH_4Cl$ affected methane consumption and growth of methanotrophs at the community level. $NH_4Cl$ caused a considerable decrease in the methane consumption rate and the expression of soluble methane monooxygenases (sMMOs) but did not inhibit the growth of Methylomonas methanica expressing sMMO. These results could be attributed to competitive antagonism of MMOs due to their direct involvement in ammonia oxidation.

Keywords

Acknowledgement

Supported by : Korea Institute of Civil Engineering and Building Technology(KICT)

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