• Journal of Plant Biotechnology
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• v.31 no.3
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• pp.191-195
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• 2004

Arabidopsis NDPK2 (AtNDPK2) is a key singaling component that regulate cellular redox state and known to enhance multiple stress tolerance when over-expressed in Arabidopsis plant (Moon et al. 2003). In order to develop transgenic potato plants with enhanced tolerance to multiple stresses, we placed an AtNDPK2 cDNA under the control of a stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system and selected on MS medium containing 100 mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of AtNDPK2 cDNA into the potato genome. When potato leaf discs were treated with methyl viologen (MV) at 10 $\mu$M, transgenic plants showed higher tolerance to MV than non-transgenic or vector-transformed plants. The NDPK2 transgenic potato plants will be further used for analysis of stress-tolerance to multiple environmental stresses.

• Korean Journal of Pharmacognosy
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• v.34 no.2 s.133
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• pp.138-141
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• 2003

In order to screen new tyrosinase inhibiting principle which is expected to be a new biofunctional skin whitening cosmetics, we have isolated 600 strains of the marine- derived fungi and investigated tyrosinase inhibiting activity for their acetone extracts. The significant activities (>70% Inhibition) were observed in the extract of 10 strains of fungi (MFA7, MFA27, MFA58, MFA317, MFA318, MFA345, MFA412, MFA552, MFA562, MFA581). These active strains were cultured in SWS medium with 1 L scale and the resulting broth and mycelium were extracted to afford mycelium extract (000M) and broth extract (000B), respectively. Tyrosinase inhibiting activity for all extracts has been tested. As the results, the broth extracts of 4 strains (27B, 58B, 552B and 581B) exhibited relatively high levels of activity of $IC_{50}$ values of $3.0-19.0\;{\mu}g/mL$. The active component of 581B was purified by assay-guided isolation to yield the known kojic acid (1), and its structure was determined by physicochemical evidence. Kojic acid showed the significant tyrosinase inhibitory activity with $IC_{50}$ values of $12.0\;{\mu}M$.

• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.976-983
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• 2003

Seven Escherichia coli cells defective with aerobic growth were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created a transcriptional fusion to lacZY. These insertion mutant cells were tested on an XG ($5-bromo-4-chloro-3-indolyl-{\beta}-D-galactopyranoside$) medium for anaerobic expression of lacZ by fusion to a promoter. The chromosomal DNA from these strains were digested by EcoRI, and the EcoRI fragments that contained the fused gene and lacZ sequence were identified by Southern hybridization, using lacZ containing plasmid as a probe. The EcoRI fragment from each strain was cloned and sequenced. The sequence data were compared with the GenBank database. The mutated gene of three strains, CYT4, CYT5, and OS11, was found to be identical, and it was nrdAB that encoded ribonucleoside diphosphate reductase. The gene nrdAB was at min 50.5 on the Escherichia coli linkage map and 2,348,084 on the physical map, and is involved in hemAe-related reduction-oxidation reaction. OS6 and OS14 mutant strains had insertion at min 8.3 and the mutated gene was hemB. The hemB encodes 5-aminolevulinate dehydratase or porphobilinogen synthase. The OS3 mutant had insertion in cydB at min 16.6. The cydAB encodes cytochrome d oxidase. In the case of OS1, the fusion was made with sucA, the E1 component of ${\alpha}-ketoglutarate$ dehydrogenase.

• Journal of Dairy Science and Biotechnology
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• v.24 no.1
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• pp.1-7
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• 2006

This experiment was performed to identify the improvement of raw milk quality after introducing raw milk grading system(1993, June). The purpose of this experiment was to investigate chemical component and microbiological quality of raw milk in jeju. This experiment made it possible to spread high standard of quality of raw milk or milk product including yoghurt, ice cream etc., and to provide dairy industry information for the construction of Jeju international free city master plan. As a result, automatic milking system is improved a lot after introducing raw milk grading system and sustained good condition compared with other provinces. High ratio was shown dairy farm in jeju for pre-milking, pre-cooling system equipment and self laboring. Otherwise, the ratio of dairy farm doing test of mastitis is low. The ratio of first grade distribution in Jeju is 80.64%, which means that was improved before introducing raw milk grading system. The number of somatic cells found in summer more than that of other seasons in raw milk. However, these data is a little higher than the nation wide data medium. Also, general components, annual lipid ratio is 3.90% that improved compared with before introducing raw milk grading system. These data showed low in summer and similar to nation wide.

• The Korean Journal of Pharmacology
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• v.17 no.2
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• pp.7-16
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• 1981

Higenamine ($Ca_{26}H_{17}No_3$. HCI, d1-1- (4-hydroxybenzyl) -6,7-dihydroxy-1,2,3,4-tetrahydroiso-quinoline hydrochloride), which has recently teen isolated from the Aconite root, was known to the cardiotonic component of the Aconite root. The positive inotropic effect of Higenamine was observed in the isolated electrically driven left atrium from rabbits with respect to the influences of extracellular calcium and of calcium antagonists, e.g. $La^{+++}$ and verapamil. A synergistic relation in the positive inotropic effect could be demonstrated between Higenamine and extra cellular calcium. The inotropic potency of $10^{-7}\;g/ml$ Higenamine was equivalant to that of 0.058 mM of calcium in the medium. In the preparation, of which contractility had been reduced by the treatment of $La^{+++}(10^{-5}-10^{-4}M)$ and verapamil$(2{\times}10^{-7}-10^{-6}M)$, Higenamine was able to restore the contractility. These results indicated that one of the possible mechanism of positive inotropism of Higenamine was to accelerate the influx of calcium from the extracellular space through the sarcolemma.

• Journal of Electrical Engineering and Technology
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• v.10 no.5
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• pp.2018-2030
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• 2015

A two stage ac drive configuration consisting of a single-phase line commutated rectifier and a three-phase voltage source inverter (VSI) is very common in low and medium power applications. The deterministic pulse width modulation (PWM) methods like sinusoidal PWM (SPWM) could not be considered as an ideal choice for modern drives since they result mechanical vibration and acoustic noise, and limit the application scope. This is due to the incapability of the deterministic PWM strategies in sprawling the harmonic power. The random PWM (RPWM) approaches could solve this issue by creating continuous harmonic profile instead of discrete clusters of dominant harmonics. Insufficient filtering at dc link results in the amplitude distortion of the input dc voltage to the VSI and has the most significant impact on the spectral errors (difference between theoretical and practical spectra). It is obvious that the sprawling effect of RPWM undoubtedly influenced by input fluctuation and the discrete harmonic clusters may reappear. The influence of dc link fluctuation on harmonics and their spreading effect in the VSI remains invalidated. A case study is done with four different filter capacitor values in this paper and results are compared with the constant dc input operation. This paper also proposes an ingenious RPWM, a ripple dosed sinusoidal reference-random carrier PWM (RDSRRCPWM), which has the innate capacity of suppressing the effect of input fluctuation in the output than the other modern PWM methods. MATLAB based simulation study reveals the fundamental component, total harmonic distortion (THD) and harmonic spread factor (HSF) for various modulation indices. The non-ideal dc link is managed well with the developed RDSRRCPWM applied to the VSI and tested in a proto type VSI using the field programmable gate array (FPGA).

• Journal of Korea Society of Industrial Information Systems
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• v.19 no.1
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• pp.53-60
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• 2014

IEEE 802.15.4 is one of the most widely adopted physical layer standards in the area of LR-WPAN(Low-Rate Wireless Personal Area Network). Numerous previous researches have contributed to deep insights on energy efficiency, transmission throughput, and reliability that IEEE 802.15.4 delivers to the LR-WPAN. As a research that is orthogonal and complementary to previous researches, we explore the implementation and practical performance evaluation of IEEE 802.15.4 MAC software. We implement the MAC software from the perspective of the networking stack, exploring the issues raised when the MAC software serves as a functional component in a complete networking stack consisting of MAC, network as well as well as application support layers. The performance is evaluated on a realistic experimental software environment integrated with operating system, networking stack, and applications.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10057-10059
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• 2015

Positron emission tomography (PET) as the functional component of current hybrid imaging (like PET/CT or PET/MRI) seems to dominate the horizon of medical imaging in coming decades. $^{18}$Flourodeoxyglucose ($^{18}FDG$) is the most commonly used probe in oncology and also in cardiology and neurology around the globe. However, the major capital cost and exorbitant running expenditure of low to medium energy cyclotrons (about 20 MeV) and radiochemistry units are the seminal reasons of low number of cyclotrons but mushroom growth pattern of PET scanners. This fact and longer half-life of $^{18}F$ (110 minutes) have paved the path of a centralized model in which $^{18}FDG$ is produced by commercial PET radiopharmacies and the finished product (multi-dose vial with tungsten shielding) is dispensed to customers having only PET scanners. This indeed reduced the cost but has limitations of dependence upon timely arrival of daily shipments as delay caused by any reason results in cancellation or rescheduling of the PET procedures. In recent years, industry and academia have taken a step forward by producing low energy, table top cyclotrons with compact and automated radiochemistry units (Lab-on-Chip). This decentralized strategy enables the users to produce on-demand doses of PET probe themselves at reasonably low cost using an automated and user-friendly technology. This technological development would indeed provide a real impetus to the availability of complete set up of PET based molecular imaging at an affordable cost to the developing countries.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.2
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• pp.250-260
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• 2007

It was investigated that the emission characteristics of volatile organic compounds (VOCs) from small and medium companies located on industrial complexes in Metropolitan area. The emission characteristics are intermittent sources in which VOCs emissions are highly depends on the working condition. Optimized ventilation system to improve air quality in working area for the three typical companies were installed. Adsorption characteristics of major VOCs such as MEK, IPA, and toluene emitted front the companies were investigated for design of the activated carbon vessel as a VOCs control facility in each company. Concentration of total hydrocarbon and gas amounts needed to ventilation were also used as a design parameter. Mixed adsorbent to improve adsorption characteristics of problematic solvents like IPA and the design guideline of the activated carbon vessel have been suggested.

• Proceedings of the Korea Contents Association Conference
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• 2007.11a
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• pp.473-477
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• 2007

Industry for artificial joint is a complex one based on various technologies, including mechanics, biotechnology, material engineering, and medicine which are connected closely. This industry has been already known to a higher value-added business, and as going into an aging society, is expected a market growth. Also, it is a knowledge based component industry and each components assembled into product is almost all import items. This characteristics made many small and medium and venture enterprises participate in this area. For these reasons, it is needed a government's promotion. In this study, commercialization environment was analyzed and then commercialization items and strategies was figured out. Firstly, three factors for need, environment, technology were analyzed and then growth drivers growth restraints were provided. Secondly, items and strategies for commercialization were analyzed based on needs and trends of technology. Commercialization opportunities are expected to be created based on strategies following as; building a integrated technology development system, making a public opinion, strengthening a marketing, entering into niche market, B2B strategies etc.