• 한국약용작물학회지
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• 제24권2호
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• pp.129-135
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• 2016

Background: In this study, we investigated the antibacterial and nitric oxide (NO) production inhibitory activities of 75% ethanol extract of Prunus sargentii branches and its fractions against acne pathogens. Methods and Results: The antibacterial activity against acne causing pathogens was determined using the disc diffusion assay. The ethyl acetate fraction showed higher activities against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. In the DPPH radical and NO scavenging assays, the butanol fraction showed strong DPPH radical and NO scavenging abilities. These activities were related to the total polyphenol and flavonoid contents of butanol fraction. On the other hand, the chloroform and ethyl acetate fractions exhibited the highest NO production inhibitory activity in Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells compared to those exhibited by other fractions. Conclusions: The extract and its ethyl acetate fraction from the branches of P. sargentii exhibited antibacterial activity and could be used as functional materials in antimicrobial related fields. Moreover, the chloroform and ethyl acetate fractions are potential antiinflammatory agents and butanol fraction acts as an effective radical scavenger.

• 한국자원식물학회지
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• 제21권3호
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• pp.196-203
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• 2008

Phytotoxicity, antioxidant activity, and cytotoxicity of the aqueous or methanol extracts from the young sprouts of the six Compositae medicinal plants were determined. Aqueous leachates at 40g dry tissue $L^{-1}$ (g $L^{-1}$) Cirsium japonicum and Aster yomena showed the highest inhibitory effect on alfalfa (Medicago sativa L.). Total phenolic content showed the highest amount in methanol extracts from Ixeris dentata, and followed by A. yomena, and Cephalonoplos segetum. Methanol extracts of C. segetum and I. dentata at 25${\mu}g$ m$L^{-1}$ exhibited the highest DPPH radical scavenging activity by 87.2, and 52.8%, respectively. By means of HPLC analysis, MeOH extracts of C. segetum had the highest amount of antioxidant chlorogenic acid. Based on MTT assay, the methanol extracts from Y. sonchifolia ($IC_{50}$ = 65.7${\mu}g$ $mL^{-1}$) showed the highest cytotoxicity against Calu-6. These results suggest that plant extracts had a dose-dependent biological potentials including phytotoxicity, antioxidant activity, and anticancer activity, and that their activities exhibited differently depending on plant species.

• 대한한방종양학회지
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• 제4권1호
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• pp.211-232
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• 1998

Because there were lots of side effects and tolerances to the existing anticancer therapeutics, the experiment extracting the anticancer effect from medicinal herbs is in progress liviely. Therefore the purpose of this study were to research the tendency and the course of anticancer studies. To research the tendency of anticancer studies, medicinal herbs of fifty three experimental papers were analyzed and to examine the course of studies, anticancer papers in the medical world were used. The obtained results were as follows: Methods of herbal medicinal treatments were elimination the pathogenic factor(祛邪) and supporting healthy energy(扶正) method used. In this study, immediately tumor bearing and immune response were the most important point. The subject of immediately tumor bearing was not in the specific cancer but in the influence on the life span of general cencerous cells. In the experimental study of immune response, the effect on NK cell activity of medicinal herbs most studied. The combined usage of medicinal herbs and anticancer agent mostly intended to know whether it inhibits the tumor cell growth. The serum test and blood cell number test show if medicinal herbs inhibit side effect of anticancer agent. More than 80 percents of used medicinal herbs, there were anticancer activities. However anticancer experimental studies using medicinal herbs two weak points. The one, it was difficult to choose a prescription according to differentiation of symptoms and signs(辨證論) of the Oriental Medicine, because we put to the test not a man but a mouse. The other, as we observed the indirect effect of the whole physiological regulation caused by synergic effects of the complex prescription, we don't understand the detailed mechanism of the herb. Therefore, if the anticancer effect of the herb is proved the experiment, we should research the concrete medical action of medicinal herbs and immunological analysis of herbal medicines to the body.

• 대한예방한의학회지
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• 제21권2호
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• pp.95-103
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• 2017

Objectives : The aim of this study is to analyze the news contents of Korean style medicinal tea and investigate how it is portrayed in the consumer market. Methods : Total of 355 articles are selected by inputting "Korean style medicinal tea" and analyzed; 108 articles for 2013, 107 articles for 2014, 140 articles for 2015. Results : 175 different tea were found from Jan. $1^{st}$ 2013 to Dec. $31^{st}$ 2015, with ginger tea the most frequently covered for 68 times. Among 175 tea, 117 had one ingredient and 58 had more than one ingredient. The most frequently covered category was "Disease and Health Promotion" followed by "Commercial Ads", "Korean style medicinal tea Culture", and others. From 355 articles, 188 provided the information on the efficacy of Korean style medicinal tea, 88 presented the pharmacological activity, 84 addressed the recipe, 80 explained the taste, and 31 included ancient literature. The most frequently covered tea were ginger tea, omija tea, gugija tea, jujube tea, mogwa tea. Autumn and winter had 109 articles, spring had 89, and summer had 86. Conclusions : This study showed the potential of Korean style medicinal tea as a successful future content. Most of the articles about Korean style medicinal tea contained information about the efficacy but practical information such as the recipe and drinking method were not addressed much. Therefore, future study is needed to investigate additional information about Korean style medicinal tea.

• 한국약용작물학회지
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• 제9권2호
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• pp.116-123
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• 2001

한인진에 대한 항산화 효과와 항균 효과를 알아보기 위하여 추출용매별 고형분 함량을 측정하여 본 결과 물 추출물에서 18. 1%로 가장 많은 고형분을 얻을 수 있었으며 메탄올, 에탄을 추출물에서는 12. 8%, 12. 6%로 별 차이가 없었다. 항산화 효과를 알아보는 방법 중 DPPH 소거효과를 이용하여 측정하여 메탄올 추출물이 $36.2{\mu}g/ml$로 가장 효과 좋았지만, 대두유에 대한 한한인진 추출물의 항산화 효과를 $60^{\circ}C$로 20일간 저장하면서 과산화물가(POV)를 측정하여 대두유를 기질로 한 저장시험 에서 1,000ppm 농도의 물추출물과 메탄올 추출물이 저장 기간이 20일 되었을 때 46.8(meq/kg, 50.8(meq/kg)의 과산화물가를 나타내 대조구의 과산화물가 79.1 (meq/kg)에 비해 낮았다. 식품의 부패균이나 식중독의 원인균으로 알려진 세균 4종을 대상으로한 항균실험에서는 추출 용매에 따른 항균력은 에탄올 추출물이 가장 좋았으며 세균에 대한 항균력으로는 Vibrio parahaemolyticus에 대해 가장 좋았다. 한인진의 간보호 효과 시험에서는 한인진 추출물을 경구투여한 V, IV군의 간무게, 체중에 대한 간무게의 비율이 생리 식염수만을 투여한 III군보다는 낮았으나 시료 채취전후로 한인진 추출물을 경구투여한 V군과 IV군사이에는 별다른 차이점이 없었다. 예방효과를 알아보기 위해 10일간 실험을 실시한 결과 한인진 물추출물만을 단독 투여한 VI군의 체중은 계속 증가하였으나 한인진 물추출물을 계속 투여한 다음 시료 채취전에 사염화탄소를 투여한 VIII군의 간무게, 체중에 대한 간비율이 대조군 VII보다 다소 감소하였다. 병리학적인 소견으로 한인진추출물을 투여 한 군에서는 사염화탄소만 투여한 군보다 간세포의 괴사와 지방적의 축적이 감소하여 사염화탄소에 의한 간독성에 효과가 있는 것으로 사료된다.

• Bulletin of the Korean Chemical Society
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• 제26권4호
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• pp.619-628
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• 2005

We attempted to replace a benzopyran ring of 4-[(N-imidazol-2-ylmethyl)-4-chloroanilino]benzopyran, previously discovered as anti-angiogenic agent with antitumor activity, with pyranopyridines. The [3,2-c]-, [3,2-b]-, [2,3-c]-, and [2,3-b]-pyranopyridines with -(imidazol-2-ylmethyl)aniline moiety at the 4-position, were synthesized respectively, and evaluated for primary anti-angiogenic properties through primary cultured HUVEC tube formation assay. From this study, we found that the pyranopyridine ring, especially [3,2-b]- and [2,3-c]-isomer, can replace the benzopyran ring of the compound 1 and can be optimized through the introduction of substituents both on the pyranopyridine ring and the aniline moiety for the identification of a novel anti-angiogenic agent.

• 생약학회지
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• 제29권3호
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• pp.237-242
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• 1998

We have tested tyrosinase inhibitory activity on the methanol extracts of 23 species marine algae and 23 species traditional medicinal plant. Among them, four medicinal plants, Ephedra sinica, Atractylodes japonica, Pinnelia ternata, and Citrus aurantium, showed strong inhibition potency over 90% at concentration of 0.33 mg/mL. Also, two marine algae, Enteromorpha compressa and Sargassum singgildianum showed mild inhibition potency over 50% at concentration of 0.33 mg/mL.

• 동아시아식생활학회지
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• 제12권3호
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• pp.249-257
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• 2002

This study was conducted to investigate the effects of medicinal plants with additives on storage characteristics of sausage. The water activity of the sausage with the additives tended to decrease with the storage time: the highest value was shown in the Angelica added sausage samples, in both cases added with dried powder as well as with extracted powder. The Paeonia extract powder added sausage had the lowest pH value. The herbal plant added sausages showed the lowest cooking and storage losses among all. The values of TBA (thiobarbituric acid value) and VBN (volatile basic nitrogen) increased with storage time, but the values of the sample with the extract powder additive were lower than those of the freeze-dry powder additive. Total plate counts of bacteria increased with storage time fur all samples, but those for the samples with Pueraria, Platycodon, Angelica, Bupleurum and Paeonia additives exhibited lower values than the control. From the above results, the sausages with medicinal plant additives showed the superior storage safety and quality characteristics.

• Bulletin of the Korean Chemical Society
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• 제26권6호
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• pp.959-965
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• 2005

Investigation of structure-activity relationships of novel quinazolines has identified 7,8-dihydro-[1,4]dioxino-[2,3-g]quinazolines as a potent inhibitor of EGFR. These compounds have a benzodioxane framwork, which was prepared by regioselective O-alkylation of ethyl 3,4-dihydroxy benzoate by epoxide ring opening. Compounds 3f and 3k were more potent than ZD-1839 in EGF enzyme and EGFR autophosporylation inhibition assays.

• Archives of Pharmacal Research
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• 제23권3호
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• pp.243-245
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• 2000

The diethyl ether extract of isodon excisus var. coreanus exhibited significant inhibitory activity in aromatase assay. Bioactivity-guided fractionation of the extract led to the isolation of three active compounds: inflexin(ent-1${\alpha}$-hydroxy-3${\beta}$,6a-diacetoxykau r-16-en-11,15-dione) (1), ursolic acid (2), and ursolic acid 3-O-acetate (3).