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Study on antimicrobial resistance and detection of MRSA of Staphylococcus aureus isolated from carcass in Daegu slaughterhouses (대구지역 도축장 출하가축의 도체에서 분리한 Staphylococcus aureus의 항생제 내성과 MRSA 검출에 관한 연구)

  • Hyun-Sook Lim;Dong-Keun Suh;Hwan-Deuk Kim;Hye-Hwa Lee;Jeong-Mi Kim;MiHa Im;Jae-Keun Cho
    • Korean Journal of Veterinary Service
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    • v.47 no.1
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    • pp.27-33
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    • 2024
  • At the present study, it was aimed to explore the states of antimicrobial resistant Staphylococcus aureus isolates from 1,360 chickens, pigs and cattle carcass (400 chickens, 480 pigs and 480 cattle) in Daegu province from January 2022 to December 2022. Among 1,360 samples, 81 of S. aureus were isolated cattle (1.4%), pigs (7.7%) and chickens (9.2%). In antimicrobial susceptibility test, all of the isolates were demonstrated susceptibility to rifampin. But the isolates were showed resistance other antibiotics in order of tetracycline (62.9%), ciprofloxacin (62.9%), tobramycin (58.0%), gentamicin (51.8%), amikacin (40.7%), penicillin (39.5%), clindamycin (35.8%), enrofloxacin (33.3%), trimethoprim/sulfamethoxazole (30.8%), oxacillin (30.8%), minocycline (29.6%), erythromycin (25.9%), quinupristin/dalfopristin (20.9%), chloramphenicol (12.3%), cefoxitin (9.8%). Among the 81 S. aureus isolates, 25 (30.8%) methicillin-resistant staphylococcus aureus (MRSA) were observed. Seven (28.0%) of 25 MRSA harbored mecA gene. About 96% of MRSA were multidrug resistance to at least 3 more drugs. A continuous monitoring and surveillance program to prevent antimicrobial resistance in livestock products is demanded.

Effect of Metastable Tetragonal $ZrO_2$ Phase on the Mechanicmal Properties in $Al_2O_3-ZrO_2$ System ($Al_2O_3-ZrO_2$계에서 기계적 성질에 미치는 준안정 저앙 $ZrO_2$상의 효과)

  • Kim, Jin-Young;Hwang, Kyu-Hong;Kim, Hwan
    • Journal of the Korean Ceramic Society
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    • v.21 no.2
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    • pp.149-155
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    • 1984
  • The effect of $ZrO_2$ dispersed phase on the mechanical properties in $Al_2O_3$-$ZrO_2$system has been studied. There are both metastable tetragonal phase and stable monoclinic phase of $ZrO_2$particles diespersed in Al2O3 matrix at room temperature. Metastable tetragonal $ZrO_2$ changes to the stable monoclinic structure within the stress field of the crack. And microcracks are formed by the expansion of $ZrO_2$during the tetragonal-monoclinic transformation on cooling. Therefore stress-induced phase transformation and inclusion-induced microcracking contribute to the mec-hanical properties of $Al_2O_3$-$ZrO_2$system. Sintered composites containing 10m/o $ZrO_2$ yield KiC values of 6.5MN/$m^{3/2}$ much greater than that of pure $Al_2O_3$ This increase results from microcrack extension and stress-induced phase transformation absor-bing energy by crack propagation. Flexural strength of composites is decreased considerably in comparison with pure $Al_2O_3$ This decrease results from microcrack as a crack former and higher porosity than pure $Al_2O_3$.

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DNA microarray-based characterization and antimicrobial resistance phenotypes of clinical MRSA strains from animal hosts

  • Schmitt, Sarah;Stephan, Roger;Huebschke, Ella;Schaefle, Daniel;Merz, Axel;Johler, Sophia
    • Journal of Veterinary Science
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    • v.21 no.4
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    • pp.54.1-54.11
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    • 2020
  • Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of severe infections in humans and animals worldwide. Studies elucidating the population structure, staphylococcal cassette chromosome mec types, resistance phenotypes, and virulence gene profiles of animal-associated MRSA are needed to understand spread and transmission. Objectives: The objective of this study was to determine 1) clonal complexes and spa types, 2) resistance phenotypes, and 3) virulence/resistance gene profiles of MRSA isolated from animals in Switzerland. Methods: We analyzed 31 presumptive MRSA isolates collected from clinical infections in horses, dogs, cattle, sheep, and pigs, which had tested positive in the Staphaurex Latex Agglutination Test. The isolates were characterized by spa typing and DNA microarray profiling. In addition, we performed antimicrobial susceptibility testing using the VITEK 2 Compact system. Results: Characterization of the 31 presumptive MRSA isolates revealed 3 methicillinresistant Staphylococcus pseudintermedius isolates, which were able to grow on MRSA2 Brilliance agar. Of the 28 MRSA isolates, the majority was assigned to CC398 (86%), but CC8 (11%) and CC1 (4%) were also detected. The predominant spa type was t011 (n = 23), followed by t009 (n = 2), t034 (n = 1), t008 (n = 1), and t127 (n = 1). Conclusions: The results of this study extend the current body of knowledge on the population structure, resistance phenotypes, and virulence and resistance gene profiles of MRSA from livestock and companion animals.

Effects of hairline treatment on surface blackening and thermal diffusion of Zn-Al-Mg alloy-coated steel sheet (Zn-Al-Mg 합금도금강판의 헤어라인 처리가 표면흑색화 및 열확산도에 미치는 영향)

  • Jin Sung Park;Duck Bin Yun;Sang Heon Kim;Tae Yeob Kim;Sung Jin Kim
    • Journal of the Korean institute of surface engineering
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    • v.56 no.1
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    • pp.69-76
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    • 2023
  • The effects of hairline treatment on surface blackening and thermal diffusion behaviors of Zn-Al-Mg alloy coated steel sheet were evaluated by the three-dimensional surface profiler and laser-flash technique. The metallographic observation of coating damages by hairline treatments showed that several cracks were initiated and propagated along the interface between primary Zn/eutectic phases. As the hairline processing became more severe, the crack occurrence frequency in eutectic phase of coating layer and the surface roughness increased, which had a proportional relationship with the level of blackening on the coating surface. In addition, the higher interfacial areas of the blackened coating surface, caused by the hairline process, led to an increase in thermal diffusivity and conductivity of the coated steel sheet. On the other hand, when the coating damage by hairline treatment was excessive and the steel substrate was exposed, there was little difference between the thermal diffusivity/conductivity of the untreated sample though the blackening degree was higher than that of untreated sample. This work suggests that the increase in the surface areas of the coating layer without exposure to steel substrate through hairline treatment can be one of the effective technical strategies for the development of Zn-Al-Mg alloy coated steel sheets with higher blackening level and thermal diffusivity.

Analysis of the Diversity of Methicillin Resistant Staphylococcus aureus (MRSA) Strains Isolated from Diarrhea Patients in Busan Area (부산지역 설사환자에서 분리한 MRSA 균주의 다양성 분석)

  • Choi, Seong-Hwa;Park, Eun-Hee;Park, Yon-Koung;Kim, Joung-A;Kim, Nam-Ho;Lee, Young-Suk;Bin, Jae-Hun;Park, Ho-Kuk
    • Journal of Life Science
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    • v.18 no.8
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    • pp.1083-1089
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    • 2008
  • We investigated the molecular epidemiological characteristic of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from stool samples in Busan from 2004 to 2006. Among 142 isolates of S. aureus, 49 isolates (34.5%) were confirmed as MRSA. With the antimicrobial susceptibility tests, 37 isolates (75.5%) showed multiple resistance to more than 10 antibiotics, but all isolates were sensitive to vancomycin. All of MRSA had enterotoxin A in 30.6%, B 4.1%, C 8.3%, D, C/G, A 2.0% and None 51%. PFGE of SmaI-digested chromosomal DNA was performed on 49 sporadic MRSA isolates. Restriction fragment patterns consisted of 8 to 14 fragments ranged in size from 48.5 to 630.5 kbp. We could divided the isolates into 7 groups ($I{\sim}VII$) by analyzing PFGE patterns. Group I subdivided as 2 subgroups and 17 (34.7%) strains belong to the group I. Dendrogram of PFGE patterns showed that MRSA strains in Busan were heterogeneous but we could find out minor homogeneity in hospital.

A Study on the Current Status of High School Students' Exposure to Pornographic Materials (고등학생들의 음란 영상매체 접촉 실태)

  • Kim Han Sook
    • Journal of Korean Public Health Nursing
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    • v.16 no.1
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    • pp.45-63
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    • 2002
  • The purpose of this study is to understand the current status of high school students' exposure to pornographic materials as well as to provide basic data to develop sex education programs. This study was conducted through a self-administered questionnaire survey of 774 students of six high schools in Seoul, three boys' and three girls' respectively, from August 22 to August 25, 2000. This study shows that high school students' exposure to pornographic materials such as CD-ROMs, internet, video tapes and adult movies has become very serious. The results of this study are as follows. 1. Among the students surveyed, $23.5\%$ of them currently has opposite-sex friends, while $42.2\%$ used to have and $34.2\%$ has never had opposite-sex friends. 2. $55.3\%$ of the students pick up their sexual knowledge from their friends, followed by mass media $(47.5\%),\;school (21.1\%),\;adult\; magazines (17.1\%)\;, internet (14.1\%)$, pornography visual $materials (11.5\%),\; sexual\; books (3.6\%)$ and pornography printed $materials (2.4\%).$ 3. Regarding the frequency of sexual education taken in school, $54.2\%$ of the students answered 'often', while $37.6\%$ answered 'almost none', 'several times' $(4.4\%)$, 'never' $(2.9\%)$ and 'frequently' $(0.9\%)$, respectively. Regarding sexual education at home, $42.2\%$ answered 'almost none', followed by 'never' $(36.3\%)$, 'often' $(16.3\%)$, 'several times' $(3.3\%)$ and 'frequently' $(1.8\%)$, respectively. 4. $68.2\%$ of the students have been exposed to adult movies, followed by pornography videos $(60.1\%)$, internet chatting $(50.2\%)$ and pornography computer diskettes $(38.0\%)$. 5. The degree of exposure to pornographic materials of boy students is much higher than that of girl students. 6. It appears that there is an interrelation between the kind of schools and the degree of the students' exposure to pornographic materials. 7. The higher the students' grade is. the more they have been exposed to pornographic materials. 8. It shows that there is an interrelation between the students' academic performance and the degree of their exposure to pornographic materials. 9. There is also an interrelation between the amount of students' pocket money and the degree of their exposure to pornographic materials. 10. There is an interrelation between the degree of their adaptation to school life and the degree of their exposure to pornographic materials. 11. It appears that there is an interrelation between their parents' relations with them and the degree of the students' exposure to pornographic materials. 12. There is a significant difference in the degree of their exposure to pornographic materials in accordance with their residential area. 13. There is also a significant difference in the degree of their exposure to pornographic materials in accordance with the average income of their families. 14. There is also a significant difference in the degree of their exposure to pornographic materials in accordance with the mean sexual education at home.

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Framework for Developing Mobile Embedded Convergence Software using CBD (컴포넌트 기반 모바일 임베디드 컨버전스 소프트웨어 개발 프레임워크)

  • Kim, Haeng-Kon
    • Journal of Internet Computing and Services
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    • v.9 no.5
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    • pp.59-72
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    • 2008
  • Computing systems in the modern era are expanding rapidly to include mobile-based businesses that make us of the various convergence distributed business process. This has lead to growing interest in the field of mobile embedded software development methodology, which has in turn lead to the proliferation of the embedded mobility. The use of CBD (Component Based Development) provides reusability, maintainability and portability, all of which are very important and focus issues to the business process. It also comes with the inherent productivity, quality and reliability of CBD. To make efficient use of CBD, though, clarified interface definitions for component integration are necessary. These definitions should be made up of collaborative hierarchical and horizontal architecture layers. Successful definitions should apply an effective framework made up of the architecture and process. In this paper, we describe an interface specification for small grained mobile embedded components(MEC) for the mobile embedded domain to meet maximum user requirements. We build and deploy the reconfigurable design patterns and components (in business domain categories) to make a component hierarchy and business logics for mobile embedded software. Proposed components specification plays a major role in development of the software for handling inconsistency in existing specification. It also includes plenty of specification information, using semantics and modeling based mechanisms to support business processes. We propose a development model of mobile embedded software using CBD for very complex and dynamic mobile business. We can apply it in a plug and play manner to develop the software. We verify that our framework supports very good productivity, quality and maintainability to meet the user's requirements in mobile business.

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Evaluation of Biogas Production Rate by using Various Electrodes Materials in a Combined Anaerobic Digester and Microbial Electrochemical Technology (MET) (미생물 전기화학 기술이 적용된 단일 혐기성소화조에서 전극재질에 따른 바이오가스 생성 효율 평가)

  • Shin, Wonbeom;Park, Jungyu;Lee, Beom;Kim, Yonggeun;Jun, Hangbae
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.2
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    • pp.82-88
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    • 2017
  • MET (Microbial Electrochemical Technology), such as MFC (Microbial Fuel Cell) and MEC (Microbial Electrolysis Cell), is a promising technology for producing sustainable biogas from an anaerobic digester (AD). At current stage, however, the most likely limiting factors, large internal resistances, should be overcome for successful scale up of this technology. Various researchers reported that application of electrode materials containing high current density, increase of ion strength and conductivity, configuration of electrode are good methods for minimizing internal resistances. Recently, stainless steel is receiving great attention because of not only high performance and durability but also low cost. Therefore, in this study, we evaluate electrochemical characteristics and biogas production rate using various electrode materials and configuration (graphite carbon coated with catalysts ($GC-C_M$) or not (GC), stainless steel mesh (SUS-M) and plate (SUS-P)). As the results, current densities of $GC-C_M$, GC, SUS-P, SUS-M were 2.03, 1.36, 1.04, $1.13A/m^2$, respectively. Methane yields of $GC-C_M$, GC, SUS-P, SUS-M were 0.27, 0.14, 0.19, 0.21 $L-CH_4/g-COD_{rem}$., respectively. Stainless steel shows high current density and methane yield, which are similar as graphite carbon coated with catalysts.

Antimicrobial resistance studies in staphylococci and streptococci isolated from cows with mastitis in Argentina

  • Elisa, Crespi;Ana M., Pereyra;Tomas, Puigdevall;Maria V., Rumi;María F., Testorelli;Nicolas, Caggiano;Lucia, Gulone;Marta, Mollerach;Elida R., Gentilini;Mariela E., Srednik
    • Journal of Veterinary Science
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    • v.23 no.6
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    • pp.12.01-12.10
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    • 2022
  • Background: Staphylococcus aureus and Streptococcus agalactiae are the main cause of clinical mastitis in dairy cattle in Argentina, whereas coagulase-negative staphylococci (CNS) and environmental streptococci are the main cause of subclinical mastitis. Bacteria isolated from infected animals show increasing antimicrobial resistance. Objectives: This study aims to determine the antimicrobial resistance of staphylococci and streptococci isolated from milk with mastitis, and to genotypically characterize the methicillin-resistant (MR) staphylococci. Methods: Isolation was performed on blood agar and identification was based on biochemical reactions. Antimicrobial susceptibility was according to the Clinical and Laboratory Standards Institute guidelines. The antimicrobial resistance genes, SCCmec type and spa type were detected by the polymerase chain reaction method. Results: We isolated a total of 185 staphylococci and 28 streptococci from 148 milk samples. Among the staphylococcal isolates, 154 were identified as CNS and 31 as S. aureus. Among the 154 CNS, 24.6% (n = 38) were resistant to penicillin, 14.9% (n = 23) to erythromycin, 17.5% (n = 27) to clindamycin, 6.5% (n = 10) to cefoxitin and oxacillin. Among the S. aureus isolates, 16.1% (n = 5) were resistant to penicillin, 3.2% (n = 1) to cefoxitin and oxacillin (MRSA). Six MR isolates (5 CNS and 1 MRSA) were positive to the mecA gene, and presented the SCCmec IVa. The MRSA strain presented the sequence type 83 and the spa type 002. Among the 28 streptococcal isolates, 14.3% (n = 4) were resistant to penicillin, 10.7% (n = 3) to erythromycin and 14.3% (n = 4) to clindamycin. Conclusions: The present findings of this study indicate a development of antimicrobial resistance in main bacteria isolated from cows with mastitis in Argentina.

Occurrence of EDC/PPCPs in Influent and Effluent of a Wastewater Treatment Plant (하수처리장 유입.유출수 내 EDC/PPCPs의 발생 특성)

  • Lee, Min-Ju;Ryu, Jae-Na;Oh, Je-Ill;Kim, Hyun-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.9
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    • pp.783-792
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    • 2009
  • This study investigated 31 selected EDCs(Endocrine Disrupting Compounds) and PPCPs(Pharmaceutical and Personal Care Products) in the influent and effluent of a wastewater treatment plant(WWTP) nearby Seoul metropolitan area. The chemical compounds of EDC/PPCPs detected from the plant influent sample include stimulant, X-ray contrast media and fire retardant. The total amount of each compound class were 59.67%, 20.20% and 9.00% respectively. However, in the effluent sample, the major micropolutants detected were oral beta-blocker(30.54%), fire retardant(20.49%), X-ray contrast media(18.17%). The EDC/PPCPs occurrence levels of this study were somewhat lower than previous domestic studies'. When compared to those of overseas, the values were even lower. Some pharmaceutical compound levels particularly measured in European studies were even several thousand times high. This study then compared PECs(Predicted Environmental Concentration) and MECs(Measured Environmental Concentration) of 9 selected pharmaceuticals compounds. The calculated PECs were substantially different with the MECs, while the occurrence order between the PECs and MECs in terms of concentrations of the compounds were similar.