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Dietary Supplementation with Acanthopanax senticosus Extract Modulates Cellular and Humoral Immunity in Weaned Piglets

  • Kong, Xiangfeng;Yin, Yulong;Wu, Guoyao;Liu, Hejun;Yin, Fugui;Li, Tiejun;Huang, Ruilin;Ruan, Zheng;Xiong, Hua;Deng, Zeyuan;Xie, Mingyong;Liao, Yiping;Kim, Sungwoo
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.9
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    • pp.1453-1461
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    • 2007
  • This study was conducted to test the hypothesis that dietary supplementation with an herbal extract of Acanthopanax senticosus (AS) enhances the immune response in weaned piglets. Sixty piglets weaned at 21 days of age were randomly assigned to 3 treatment groups representing the addition of 0 or 1 g/kg of the AS extract or 0.2 g/kg of colistin (an antibiotic) to maize- and soybean meal-based diets (n = 20 per group). On days 7, 14 and 28 after initiation of the addition, total and differential counts of leucocytes, proliferating activity of peripheral lymphocytes, serum levels of immunoglobulins (Ig) and cytokines and the spleen index were determined. The AS extract decreased (p<0.05) the number of neutrophils on days 7 and 28 in comparison with the control group and reduced (p<0.05) serum interleukin-$1{\beta}$ level on day 28 compared with the other 2 groups. Dietary supplementation with the AS extract increased (p<0.05) the lymphocyte/leukocyte ratio on day 28 compared with the control group and increased the proliferating activity of lymphocytes on days 14 and 28 compared with the other 2 groups. The AS extract increased (p<0.05) the serum content of IgG on day 7 and of IgG and IgM on day 28 compared with the other 2 groups, as well as increasing the serum content of tumor necrosis factor on day 7 and spleen index on days 7 and 28 compared with the control group. Collectively, these findings suggest that the AS extract as a dietary additive enhances the cellular and humoral immune responses of weaned piglets by modulating the production of immunocytes, cytokines and antibodies.

Effect of Sterols on Phytophthora infestans and Oospore Production on detached Potato Plants (감자 역병균에 대한 스테롤류의 영향 및 감자절편에서의 난포자 형성)

  • 이왕휴;이용훈;이두구
    • Korean Journal of Plant Resources
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    • v.14 no.1
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    • pp.8-14
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    • 2001
  • The effects of media, cholesterol, $\beta$-sitosterol and lecithin on the growth and oospore production of the isolates KM10, U6, CDB6, MHB6, JD1 (A$^2$type) of Phytophthora infestans isolated in Korea and F8l7, DNC303 (A$^1$type), IB908, DN107 (A$^2$type) obtained from Japan were investigated. Mycelium of P. infestans grew better on V-8 juice agar and rye meal agar than on the other media. Oospores were produced most abundantly on V-8 juice agar. Mycelium extended more 16.6, 8.3, and 5.2% on V-8 juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively, and oospores are produced 76.0, 58.0, and 34.6 % on V-8juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively. Oospores more produced on detached potato plant disks when $A^1$ and $A^2$ type exist simultaneously which indicating that variation of population can occur in the field, but the rate of oospore formation and the number of oospores produced was low and small quantity.

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A Survey of College Students Opinions on School Cafeteria in Northern Kyungkido Area (경기 북부 지역 대학생들의 학교식당 이용실태)

  • 황윤경;이지호;김수희
    • The Korean Journal of Food And Nutrition
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    • v.10 no.2
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    • pp.201-207
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    • 1997
  • This survey was based on the college students opinions on their cafeteria in northern Kyungkido area during three weeks(5th Nov.∼21st Nov., 1996). The rate of the daily usage of the college cafeteria was 64.29% in day time class, 44.35% in night time class. The first reason of using the college cafeteria was 'no other place to eat' in day time class(49.38%) and night time class(68.01%). The second reason was 'to be economical' and the third was 'to save the time'. The main reason for not using the college cafeteria was 'bad taste' in day time class(52.09%) and night time class(31.99%). The percentage of the students who ate outside of the college in day time class was 68.63% and night time class was 37.37, 31.18% of the night time class ate the dinner at home after school. The average type of the meal were : the boiled rice, noodles, cookies and bread(from the most to the least). The students survey showed rather dissatisfactory response on taste, preference, the number of the side dishes except the quantity of the food. The necessary improvements for the college cafeteria were, first, 'improve the quality and taste of the food' and 'increase the variety of the menu', 'lower the price' and 'sanitation' were followed.

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Mineral Metabolism in Singleton and Twin-pregnant Dairy Goats

  • Harter, C.J.;Castagnino, D.S.;Rivera, A.R.;Lima, L.D.;Silva, H.G.O.;Mendonca, A.N.;Bonfim, G.F.;Liesegang, A.;St-Pierre, N.;Teixeira, I.A.M.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.1
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    • pp.37-49
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    • 2015
  • During pregnancy, the maternal body undergoes significant physiological changes. The present study assessed the changes on calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na) and potassium (K) metabolism in singleton and twin-pregnant dairy goats. The 42 goats used ($49.5kg{\pm}7.6$ body weight [BW]) were assigned at random to treatments that were factorially arranged to account for 2 breeds (Oberhasli and Saanen), 2 pregnancy types (singleton and twin) and 3 gestation periods (80, 110, and 140 days). Digestibility trials were performed at 80, 110, and 140 days of gestation. Mineral retention during pregnancy was determined in the maternal body, femur, uterus, mammary gland, fetus and fetal fluid. Blood samples were taken during pregnancy before and after a meal, and Ca, P, Mg, Na, K ions and alkaline phosphatase activity determined in serum. Bone mineral density was determined in the right femur. Statistical analyses were performed using the SAS MIXED procedure. Dry matter intake decreased linearly up to 140 days of gestation. Maternal BW gain, and Ca, P, and Mg retention (g/kg) decreased linearly with the advance of gestation days. Macromineral retention in maternal body (g/kg) was greater in Oberhasli than Saanen goats, and their fetuses had higher Ca, P, and Mg deposition (mg/g). Mineral retention (mg/g) increased in fetuses according to pregnancy development, with no differences between singleton and twin pregnancy. In the mammary gland, the retention of all minerals (g) increased with the days of pregnancy. In conclusion, related to Ca, P, and Mg metabolism can be divided into two stages. Up to 80 days of gestation, was characterized by the preparation of the maternal body reserves for future mineral demands. From 80 days of gestation onward, was characterized by the transfer of maternal body reserves for fetal development and colostrum production. Na and K supply was provided by adjustments in endogenous excretion and an increase in intestinal absorption. Finally, mineral metabolism was specific to each genotype and, except for Na, was not affected by the number of fetuses.

Further Modifications to the Mobile Nylon Bag Technique to Determine Nutrient Digestibility for Swine

  • Thacker, P.A.;Qiao, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.8
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    • pp.1149-1156
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    • 2001
  • Previous studies conducted with swine have reported that the mobile nylon bag technique (MNBT) does not always accurately predict in vivo nutrient digestibilities. Therefore, in this study, the MNBT was modified so that nutrient digestibilities would more closely resemble those from conventional (Con) digestibility studies obtained using the indicator method. A total of 19 feeds were tested including five cereal grains, five legumes, three high protein sources and six mixed diets. The principle changes to the MNBT included the use of a fecal collection harness which minimized the number of bags lost. In addition, previous protocols involved pooling of bags within pig while in the present experiment all bags were analyzed separately to increase the precision of the test. Finally, chemical analyses were done using the entire nylon bag plus residue rather than opening.the bags and scraping out the contents. With the exception of the barley sample (p=0.01), dry matter digestibility (DMD) coefficients obtained with the MNBT were not significantly different from those obtained with the indicator method. The linear regression equation relating the MNBT to the indicator method was Con DMD=-O.77+1.02 MNBT DMD ($r^2=0.93$: p<0.0001). There was no significant (p>0.05) difference in gross energy digestibility (GED) coefficients determined using the MNBT or the indicator method for any of the 19 feeds. The regression line equation relating the MNBT to the indicator method was Con GED=-5.68+1.06 MNBT GED ($r^2=0.94$: p<0.0001). The MNBT was less effective in predicting in vivo crude protein digestibility (CPD) than it was in predicting dry matter and energy digestibility. Differences greater than five percentage units were observed for two of the legumes, Kabuli chickpeas (p=0.02) and the extruded pea-canola seed mixture (p=0.01) as well as for three of the mixed diets including the unheated hulled barley-based diet (p=0.01), the unheated hulless-barley based diet (p=0.08) and the barley-soybean meal based diet (p=0.008). The regression equation relating the MNBT to the indicator method was Con CPD=5.75 + 0.90 MNBT CPO ($r^2=0.76$; p<0.0001). This study indicates that the modified MNBT can be used for the rapid determination of dry matter and energy digestibility in a wide variety of ingredients. For the measurement of crude protein digestibility, the technique produces results similar to conventional digestibility studies for cereal grains and high protein feeds but tends to overestimate protein digestibility for legumes and mixed diets.

Screening in the Era of Economic Crisis: Misperceptions and Misuse from a Longitudinal Study on Greek Women Undergoing Benign Vacuum-assisted Breast Biopsy

  • Domeyer, Philip John;Sergentanis, Theodoros Nikolaos;Katsari, Vasiliki;Souliotis, Kyriakos;Mariolis, Anargiros;Zagouri, Flora;Zografos, George Constantine
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.9
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    • pp.5023-5029
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    • 2013
  • Background: To evaluate knowledge about screening tests and tests without proven screening value in a Greek Breast Unit population undergoing benign vacuum-assisted breast biopsy (VABB). Materials and Methods: This study included 81 patients. Three knowledge-oriented items (recommended or not, screening frequency, age of onset) were assessed. Regarding screening tests two levels of knowledge were evaluated: i). crude knowledge (CK), i.e. knowledge that the test is recommended and ii). advanced knowledge (AK), i.e. correct response to all three knowledge-oriented items. Solely CK was evaluated for tests without proven screening value. Risk factors for lack of knowledge were assessed with multivariate logistic regression. A second questionnaire was administered 18 months after VABB to assess its impact on the performance of tests. Results: Concerning screening tests considerable lack of AK was noted (mammogram, 60.5%; Pap smear, 59.3%; fecal occult blood testing, 93.8%; sigmoidoscopy, 95.1%). Similarly lack of CK was documented regarding tests without proven screening value (breast self-examination, 92.6%; breast MRI, 60.5%; abdominal ultrasound, 71.6%; barium meal, 48.1%; urine analysis, 90.1%; chest X-Ray, 69.1%; electrocardiogram, 74.1%; cardiac ultrasound, 75.3%). Risk factors for lack of AK were: place of residence (mammogram), age (Pap smear), personal income (sigmoidoscopy); risk factors for lack of CK included number of offspring (breast MRI, chest X-Ray), BMI (abdominal ultrasound), marital status (urine analysis), current smoking status (electrocardiogram). VABB's only effect was improvement in mammogram rates. Conclusions: A considerable lack of knowledge concerning screening tests and misperceptions regarding those without proven value was documented.

Effects of Hermetia Illucens Supplementation on Fecal Score, Blood Profiles, Immune Response and Small Intestinal Morphology in Weaned Pigs (이유자돈 사료 내 동애등에(Hermetia illucens)의 첨가가 분변지수, 혈액성상, 면역반응 및 소장형태에 미치는 영향)

  • Choi, Yo-Han;Kim, Jo-Eun;Jung, Hyun-Jung;Cho, Eun Seok;Kim, Dong-Woo;Kim, Jin-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.4
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    • pp.392-399
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    • 2020
  • This study was undertaken to determine the effects of feed supplementation with Hermetia illucens (HI) on the fecal score, blood profiles, immune response, and small intestinal morphology in weaned pigs. A total of 24 weaned pigs (Landrace×Yorkshire×Duroc; 5.86±0.13 kg) were randomly allotted to 4 treatments and 6 replicates on the basis of initial body weight. The experiment was accomplished over 0-14 days. The dietary treatments included a corn-soybean meal diet supplemented with 0, 1, 2, and 3% HI. A linear response to increasing dietary HI was observed for the number of monocytes (p<0.01) and eosinophils (p<0.05), whereas red blood cells tended to decrease with increasing HI levels. Plasma TNF-α levels were also determined to linearly decrease with HI supplementation (p=0.07). Moreover, a linearly decreasing tendency (p=0.06) was observed in the fecal score with increasing dietary levels of HI. Weaned pigs fed diets supplemented with increasing dietary concentrations of HI showed linearly improved (p<0.05) duodenal villus height during the study period. Taken together, these results indicate the beneficial effects of HI on diarrhea reduction, immune response, and small intestinal morphology in weaned pigs.

A Study of Diabetic Control for Diabetic Patient in $^{18}F$-FDG PET ($^{18}F$-FDG PET 검사를 위한 당뇨 환자의 혈당 조절에 관한 연구)

  • Cha, Min-Kyeong;Nam, Ki-Pyo;Lee, Young-Hee;Choi, Jae-Min;Cho, Shee-Man
    • The Korean Journal of Nuclear Medicine Technology
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    • v.12 no.1
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    • pp.57-61
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    • 2008
  • Purpose: $^{18}F$-FDG PET is to evaluate the tumor using glucose metabolism. Blood Glucose Level (BGL) is important factor that affects on a result of examination. But it often appears the distort result on diabetic patient due to a failure of BGL control. The aim of this study is to make a effective guideline for diabetic patient prescribed $^{18}F$-FDG PET and improve accuracy and reliability of examination. Materials and methods: A subject of study is 69 diabetic patients and divided them into 3 groups; 1) BGL is less than 120 mg/dl & performed PET, 2) BGL is over than 120 mg/dl & performed PET, 3) BGL is over than 120 mg/dl & not performed PET. And we investigated the type of diabetic medications and the time of dosage, whether they have meal or not. And we analyzed SUV of liver and mediastinum to recognize the effect of BGL on PET images. Results: As a methods to diabetic control, Amalyl showed high percentage of 27.5% in oral medication and Humulin R showed 14.5% in insulin. Their peak time was 2-3 hrs, 2-4 hrs and duration time was 24 hrs, 5-7 hrs. The number of that had a breakfast was 16, 7 mens (43.8%) couldn't perform PET as over 120 mg/dl and 5 mens (31.3%) performed PET as less than 120 mg/dl after they took a medication. When we set the SUV of normal for 1.5-2.0, 1.0-1.5 on liver and mediastinum, the percentage out of the range was 31.8%, 52.9%, 62.5% in 120-139 mg/dl, 140-159 mg/dl, 160-200 mg/dl respectively. Conclusions: We completed standardized guideline for diabetic patients. As a result of this study, improved customer satisfaction and increased economic benefits. It is expect to be a effective model in other PET centers.

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Assessment of Food Service Management Practices in Child Care Centers Operated by Various Types of Foundations in the Chonbuk Area of Korea (전북지역 보육시설의 시설유형에 따른 급식운영관리 실태 조사)

  • Rho, Jeong-Ok;Lee, Eun-Pa;Lee, Jin-Sook
    • Korean journal of food and cookery science
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    • v.25 no.1
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    • pp.74-83
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    • 2009
  • We investigated the food service management practices in child care centers operated by various types of foundations in the Chonbuk area of Korea. Self-administered questionnaires were collected from 125 child care center directors. The statistical analysis was completed using an SPSS v11.5 program. Approximately 82.4% of the directors were women with an the average age of 45 years old. The average number of children in each public and private child care center was 84.7 and 88.8, respectively (P<.001), and the difference in time of operation was significant(P<.001). Only 20.8% of the centers employed a dietitian, whereas 92.0% of the centers employed a cook, thus, food service was not managed by professionals in most centers. In approximately 99.2% of the centers, meals were prepared in a conventional manner. The difference in daily meal frequency was significant(P<.05), with approximately 85.6% of the centers serving snacks twice a day primarily to supplement the intake of nutrients(4.44 point) and to add fun to the daily lives of the children(4.12 point). Approximately 40.7% of the directors of public centers and 57.6% of the directors of private centers responded that the most difficult aspect of food service management was financial management. Overall, 56.8% of the directors responded that the details of financial management had the greatest effect on their ability to improve the quality of food service. In most centers, the center director was also the purchasing manager and half of them purchased food every day. Approximately 97% of the directors responded that they do not include instant foods in their menu plans but they would use them if the children wanted to eat them. These results indicate that the food service management in child care centers in the Chonbuk area is relatively inadequate. We strongly recommend that they hire dietitians for food service administration and focus on strengthening sanitation management.

A study on the oral health behavior of oral prophylaxis clients (스켈링 실습실 방문자의 구강건강행위에 관한 연구)

  • Jang, Gye-Won;Kang, Yong-Ju;Jeong, Mi-Kyoung
    • Journal of Korean society of Dental Hygiene
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    • v.10 no.6
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    • pp.1083-1093
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    • 2010
  • Objective : The purpose of this study was to examine the relationship of the oral health behavior of oral prophylaxis clients and some residents in a community to their simplified oral environment index. Methods : The subjects in this study were 520 people who had their teeth cleaned in the oral prophylaxis practice lab in the department of dental hygiene at J health college. A survey was conducted from March 23 to June 3, 2010, by way of the self-reported questionnaire. The data materials are analyzed by general characteristics frequency and ratio, the relationship of oral health behavior and sex, age, scaling experience, simplified oral hygiene index $x^{2}$ test analysis. Results : 1. Concerning links between gender and oral health behavior including daily toothbrushing frequency, the largest group of the respondents brushed their teeth three times a day(p<0.05). As to educational experiences on toothbrushing method and the use of oral hygiene supplies, the women had more educational experiences than the men(p<0.05). 2. Regarding connections between age and oral health behavior involving daily toothbrushing frequency, the largest number of the respondents brushed their teeth three times a day in every age group (p <0.001). As for educational experiences on toothbrushing method, those who were in their 50s and up(64.7%) learned about that, and they had more educational experiences with age(p<0.05). As for scaling experiences, the older respondents had their teeth scaled more often (p<0.001). 3. As to relationship between scaling experiences and oral health behavior, there were differences in toothbrushing frequency according to scaling experiences(p <0.05). Regarding educational experiences on toothbrushing method, those who had their teeth cleaned received more toothbrushing education(p<0.001). Concerning smoking, the nonsmokers had more experiences to get their teeth cleaned (p<0.001). 4. As for links between simplified oral environment index and oral health behavior including a time for toothbrushing, the respondents who did toothbrushing after every meal(80.4%) had good simplified oral environment indexes(p<0.05). 5. In regard to relationship between simplified oral environment index and oral health behavior, oral environment index had a positive correlation to the use of oral hygiene supplies( r=0.129**), toothbrushing time(r=0.116**) and educational experiences on toothbrushing method (r=0.099**). Smoking(r=-0.092**) had a negative correlation to that. Conclusion : The above-mentioned findings illustrate that oral health behavior is one of crucial factors to affect oral health status and oral environment care. Therefore oral prophylaxis practice lab visitors should receive education on the right toothbrushing method and the use of oral hygiene supplies to promote their oral health, and an incremental oral health care system that involves regular scaling should be introduced.