• Biomolecules & Therapeutics
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• v.21 no.2
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• pp.146-152
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• 2013

This study examined the total polyphenol content of eight wild edible plants from Ethiopia and their effect on NO production in Raw264.7 cells. Owing to its relatively high polyphenol concentration and inhibition of NO production, the methanol extract of Adansonia digitata L. leaf (MEAD) was subjected to detailed evaluation of its antioxidant and anti-inflammatory effects. Antioxidant effects were assessed by measuring free-radical-scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and oxygen-radical-absorbance capacity (ORAC) assays, while anti-inflammatory effects were assessed by measuring inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. In the ORAC assay, MEAD was 10.2 times more potent than vitamin C at eliminating peroxyl radicals. In DPPH assay, MEAD also showed a strong ROS scavenging effect. MEAD significantly inhibited iNOS activity ($IC_{50}=28.6{\mu}g/ml$) of LPS-stimulated Raw264.7 cells. We also investigated the relationship between iNOS expression and nuclear factor kappa B (NF-${\kappa}B$) activation. MEAD inhibited $I{\kappa}B{\alpha}$ degradation and NF-${\kappa}B$ translocation from the cytosol to the nucleus in LPS-induced RAW264.7 cells without significant cytotoxic effects, as confirmed by MTT assay. These results suggest that MEAD inhibits anti-inflammatory iNOS expression, which might be related to the elimination of peroxyl radicals and thus the inhibition of $I{\kappa}B{\alpha}$-mediated NF-${\kappa}B$ signal transduction.

• Smart Structures and Systems
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• v.17 no.6
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• pp.957-980
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• 2016

Structural damage detection (SDD) is a challenging task in the field of structural health monitoring (SHM). As an exploring attempt to the SDD problem, a hybrid self-adaptive Firefly-Nelder-Mead (SA-FNM) algorithm is proposed for the SDD problem in this study. First of all, the basic principle of firefly algorithm (FA) is introduced. The Nelder-Mead (NM) algorithm is incorporated into FA for improving the local searching ability. A new strategy for exchanging the information in the firefly group is introduced into the SA-FNM for reducing the computation cost. A random walk strategy for the best firefly and a self-adaptive control strategy of three key parameters, such as light absorption, randomization parameter and critical distance, are proposed for preferably balancing the exploitation and exploration ability of the SA-FNM. The computing performance of the SA-FNM is evaluated and compared with the basic FA by three benchmark functions. Secondly, the SDD problem is mathematically converted into a constrained optimization problem, which is then hopefully solved by the SA-FNM algorithm. A multi-step method is proposed for finding the minimum fitness with a big probability. In order to assess the accuracy and the feasibility of the proposed method, a two-storey rigid frame structure without considering the finite element model (FEM) error and a steel beam with considering the model error are taken examples for numerical simulations. Finally, a series of experimental studies on damage detection of a steel beam with four damage patterns are performed in laboratory. The illustrated results show that the proposed method can accurately identify the structural damage. Some valuable conclusions are made and related issues are discussed as well.

• Journal of Microbiology and Biotechnology
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• v.22 no.7
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• pp.939-946
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• 2012

Antibiotic production with Streptomyces sindenensis MTCC 8122 was optimized under submerged fermentation conditions by artificial neural network (ANN) coupled with genetic algorithm (GA) and Nelder-Mead downhill simplex (NMDS). Feed forward back-propagation ANN was trained to establish the mathematical relationship among the medium components and length of incubation period for achieving maximum antibiotic yield. The optimization strategy involved growing the culture with varying concentrations of various medium components for different incubation periods. Under non-optimized condition, antibiotic production was found to be $95{\mu}g/ml$, which nearly doubled ($176{\mu}g/ml$) with the ANN-GA optimization. ANN-NMDS optimization was found to be more efficacious, and maximum antibiotic production ($197{\mu}g/ml$) was obtained by cultivating the cells with (g/l) fructose 2.7602, $MgSO_4$ 1.2369, $(NH_4)_2PO_4$ 0.2742, DL-threonine 3.069%, and soyabean meal 1.952%, for 9.8531 days of incubation, which was roughly 12% higher than the yield obtained by ANN coupled with GA under the same conditions.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.327-327
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• 2018

돌발홍수, 집중호우 등 강우가 발생 원인되는 자연재해에 효과적으로 대응하기 위한 연구가 활발히 이루어지고 있으나 강우의 시공간 변동성과 발생과정의 복잡한 물리과정으로 인해 강우 추정에 한계를 가진다. 일반적으로 강우 추정은 물리적, 추계학적 모형을 이용하며 추계학적 모형의 점과정(point process)을 이용하여 강우를 생산한다. 추계학적 강우 모형은 관측 강우의 시간 스케일, 강우발생 빈도, 강우 강도 등 강우 구조의 특성을 반영 할 수 있다는 장점을 가지고 있으나 생산되는 강우의 구조가 추정되는 매개변수에 크게 의존한다는 점에서 실제 강우에 적합한 매개변수 추정이 중요하다. 본 연구에서는 낙동강 유역내에 있는 20개의 강우관측 지점을 대상으로 1973년-2017년까지의 강우 관측자료를 수집하였으며 추계학적 강우생성 모형으로 점과정을 이용하는 추계학적 강우생성 모형인 NSRPM(Neymann-Scott rectangular pulse model)을 선정하였다. NSRPM모형의 매개변수를 추정하기위한 최적기법으로 DFP(Davidon-Fletcher-Powell), GA(genetic algorithm), Nelder-Mead, DE(differential evolution)를 이용하여 추정된 매개변수의 적합성을 분석하고 지역특성을 고려한 매개변수 추정 기법을 제시하였다. 추정된 모형의 매개변수를 분석한 결과 DE와 Nelder-Mead 기법이 높은 적합성을 보였으며 DFP, GA기법이 상대적으로 낮은 적합도를 보였다.

• Journal of the Korean Institute of Telematics and Electronics
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• v.25 no.5
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• pp.509-521
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• 1988

In the designs of integrated circuits, the buffer circuits used for driving a large capacitive load from minimum-structured logic circuit outputs have important effects upon system throughputs. Therefore it is important to optimize the buffer circuits. In this paper, the principle of designing CMOS buffer circuits which have the minimum delay and drive the given capacitive load is discussed. That is, the effects of load capacitance upon rise time, fall time, and delay of the CMOS inverter and the effects of parasitic capacitances are finely analysed to calculate the requested minimum-delay CMOS buffer condition. This is different from the method by C.A. Mead et. al.[2.3.4.]which deals with passive-load-nMOS buffers. Large channel width MOS transistor stages are necessary to drive a large capacitive load. The effects of polysilicon gate resistances of such large stages upon delay are also analysed.And, the area of buffer circuits designed by the proposed method is smaller than that of buffer circuits designed by C.A. Mead's method.

• Journal of Industrial Technology
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• v.22 no.A
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• pp.19-28
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• 2002

The Nelder-Mead simplex algorithm has been one of the most widely used methods for the nonlinear unconstrained optimization, since 1965. Recently, the new algorithm, (so-called the Fuzzy Simplex Algorithm), with fuzzy logic controllers for the expansion, reflection and contraction process of this algorithm has been proposed. In this paper, this new algorithm is developed. And, the formulation for the optimum design of the power screw's efficiency is made. And then, the developed fuzzy simplex algorithm as well as the original one is applied to this optimum design problem. The Fuzzy simplex algorithm results in a faster convergence in this problem, as reported in other study, too.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.05a
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• pp.978-981
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• 2005

The vibration analysis of damped sandwich beam is conducted using finite element method. The equation of motion presented by Mead and Markus is used to formulate FEM. Also as the thickness of the core in the damped sandwich beam goes to zero, conventional beam theory based on the transformed-section method and the equation of Mead and Markus are compared. According to the change of thickness and loss factor of the core, the forced frequency response of beam is calculated and discussed. And then using the half-power band width method, the damping ratio of each mode is calculated and discussed about each case.

• Journal of the Korea institute for structural maintenance and inspection
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• v.3 no.4
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• pp.139-144
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• 1999

Based on the recent developments of the reliability-based structural analysis and design as well as the extending knowledge on the probabiliistic characteristics of load and resistances, the probability based design criteria have been successfully developed for many standards. Since the probabilistic characteristics depend highly on the local load and resistances, it is recognized to develop the design criterion compatible with domestic requirements. The existing optimum design methods, which are generally based on the structural theory and certain engineering experience, do not realistically consider the uncertainties of load and resistances and the basic reliability concepts. This study is directed to propose a optimum design based Expected Total Cost Minimization on P.S.C Box Girder Bridge system which could possibly replace optimum design based traditional provisions of the current code, based on the Neldel-Mead Method reliability theory.

• Journal of Hydrospace Technology
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• v.2 no.2
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• pp.24-35
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• 1996

This paper describes parameter identification techniques formulated for the estimation of maneuvering coefficients of a submerged body. The first part of this paper is concerned with the identifiability of the system parameters. The relationship between a stochastic linear time-invariant system and the equivalent dynamic system is investigated. The second is concerned with the development of the numerically stable identification technique. Two identification techniques are tested; one is the ma7mum likelihood (ML) methods using the Holder & Mead simplex search method and using the modified Newton-Raphson method, and the other is the modified extended Kalman filter (MEKF) method with a square-root algorithm, which can improve the numerical accuracy of the extended Kalman filter. As a results, it is said that the equations of motion for a submerged body have higher probability to generate simultaneous drift phenomenon compared to general state equations and only the ML method using the Holder & Mead simplex search method and the MEKF method with a square-root algorithm gives acceptable results.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.10a
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• pp.216-216
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• 2003