• Bulletin of the Korean Chemical Society
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• 제24권7호
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• pp.943-947
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• 2003

We have studied the migration behavior of single-stranded DNA using capillary gel electrophoresis under various conditions. It was found that optimum electric fields should be less than 150 V/cm for the good tradeoff between the separation time and the resolution. It seems that the gel matrix with the combination of different polymer average molecular weights is important to extend the maximum readable DNA bases. The total gel concentration less than 3.1% in the mixed gel system showed good separation efficiency up to 600 bases. The best result was obtained with the poy(ethylene)oxide (PEO) gel concentration of 1.2% of Mr 8,000,000 and 1.8% of Mr 600,000. We observed that the capillary length between 50 cm to 100 cm (effective length) should be employed for the optimization between the total DNA migration time and the maximum readable length. A trizma base-boric acid-ethlyenediaminetetraacetic acid (EDTA) (TBE) buffer was commonly used for DNA sequencing, but we found that 3-[tris(hydroxymethyl)methyl amino]-1-propane sulfonic acid (TAPS) buffer worked as well for the single-stranded DNA separation. Especially, TAPS buffer showed a good resolution for very short DNA bases (1 to 30 bases).

• Industrial Engineering and Management Systems
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• 제3권2호
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• pp.129-133
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• 2004

Studies on the optimal location of retail store have been made in case of no obstacle(Minagawa etal. 1999). This paper deals with the location problem of retail store considering obstacles (e.g. rivers, railways, highways, etc.) and obstacle-overcoming points (e.g. bridges, railway crossings, zebra crossings, overpasses, etc.). We assume that (1) commercial goods dealt here are typically convenience goods, (2) the population is granted as potential demand, (3) the apparent demand is a function of the maximum migration length and the distance from the store to customers, (4) the scale of a store is same in every place and (5) there is no competitor. First, we construct the basic model of customers' behavior considering obstacles and obstacle-overcoming points. Analyzing the two dimensional model, the arbitrary force attracting customers is represented as a height of a cone where the retail store is located on the center. Second, we formulate the total demand of customers and determine the optimal location that maximizes the total demand. Finally, the properties of the optimal location are investigated by simulation.

• 한국패류학회지
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• 제32권4호
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• pp.241-247
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• 2016

Macrophage Migration Inhibitory Factor (MIF) are well-defined role as unique cytokine and critical mediator in acute and chronic inflammatory diseases, autoimmune diseases. In this study, we isolated and characterized a full-length of MIF cDNA from the abalone (Haliotis discus hannai). The full-length cDNA of abMIF was of 1264 bp, consisting of a 5'-terminal UTR of 143 bp, an open reading frame of 360 bp and a 3-terminal UTR of 761 bp. The abalone MIF cDNA encodes a 119-amino acid polypeptide with a calculated molecular mass of 13.4 kDa and isoelectric point of 9.07. Multiple alignments and phylogenetic analysis with the deduced abalone MIF protein and showed strong homology with disk abalone (Haliotis discusdiscus). The deduced amino acid sequence of abMIF exhibited homology with other reported MIFs, such as 80%, with that of other disk abalone H. discus discus MIF gene. Quantitative real-time PCR (qRT-PCR) analysis indicated that abMIF was highly expression observed in hapatopacreas, intestine, foot, and gonad of normal conditioned abalone. Even though AbMIF mRNA level in hemocytes was low under the normal condition, it was sharply up-regulated and reached the maximum at 6 h post-infection with Vibrio parahaemolyticus, and then decreased at 24 h post-infection. This result indicates that abMIF plays an important role in responding in the innate immune system.

• 한국환경복원기술학회지
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• 제14권3호
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• pp.157-168
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• 2011

In order to promote efficiency of upstream and downstream migration of fishes, this study has developed a combined fishway of herring-bone bottom baffle type and brush type fishways. The results obtained are as follows : 1. In a channel with constant incline, the velocity of current generally shows a distinct tendency of acceleration as it goes down the stream. But in the hydraulic experiment of herring-bone bottom baffle type fishway, the velocity reached its maximum only at 0.4m/sec, and it tended to be stable without any acceleration. 2. The velocity in the brush type fishway showed a distinct tendency of acceleration as the discharge increased. But its greatest velocity was only 0.3m/sec, and its velocity change according to the discharge increase was only 0.15m/sec at maximum. 3. The maximum velocity in the combined type fishway was less than half of the blast speed of the poorest swimmer, the juvenile eel with 90mm of body length. So any species of fishes are supposed to be able to migrate upstream from the estuary through this combined type fishway. 4. The field experiment of upstream migration showed that the combined type fishway can promote efficiency of upstream and downstream migration of any species of fishes.

• Parasites, Hosts and Diseases
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• 제23권2호
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• pp.247-252
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• 1985

In the present study, the effect of primary infection to reinfection with Ascaris suum larvae was experimented in mouse model. Mice were challenged with 1,000 infective stage eggs of Ascaris suum. The embryonated eggs were directly introduced into stomach of mice. Reinfection was performed at 50 days after the primary infection with same method as primary infection. Mice were sacrificed 3, 5, 7, 10, 15 and 20 days after infection in both groups respectively. Larvae collected from livers and lungs with Baermann's apparatus were enumerated and measured after sacrifice. Sera of mice were also collected at same time. The results of the experiment were as follows: With antigen prepared from coelomic fluid of adult Ascaris suum and sera collected from mice before reinfection, the production of antibody in experimental mice was confirmed by the gel-diffusion technique. In the livers of reinfected mice, the larvae were recovered up to 10 days after challenge, otherwhile in the primary infected mice, the larvae were observed up to 7 days. The maximum number of larvae were observed in the lungs of primary infected mice on 10 days after inoculation. In the lungs of reinfected mice, maximum number of larvae were recovered on 7 days after, only few larvae were recovered on 10 days after reinfection. As regards the growth of the larvae, the third stage larvae, over $500{\mu\textrm{m}}$ in length, appeared in livers at 5 days after reinfection, but it couldn't be found on 7 days and 10 days after challenge. The third stage larvae continuously developed were observed in lungs of mice from 5 days after reinfection. In conclusion, it was found that development of larvae in livers of immune mice were probably repressed by the immune mechanisms being rises in livers and defence mechanism is also acting by interfering with the process of larval penetration into the lung from the liver.

• 한국결정성장학회지
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• 제10권2호
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• pp.122-127
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• 2000

불규칙 입자형성을 갖는 Ce-TZP와 알루미나가 분산된 Ce-TZP 세라믹스를 세라아 도핑조선과 열처리 조건을 변화시켜 제조한 다음, 미세구조를 관찰하였다. 제조된 시편들은 상대밀도가 99% 이상인 고밀도의 소결체였으며, 정방정 및 입방정상 지르코니아 입자로 구성되었다. 도핑하지 않거나 소결만 시킨 시편의 경우 직선적인 입계와 정상적인 입자형성을 나타낸데 비하여 세리아를 침적법으로 도핑한 후 고온으로 열처리한 시편에서는 확산구동 입계이동이 일어나 입계 및 입자형성이 불규칙하였으며. 이러한 Ce-TZP에서는 입자당 평균 입계같이 정상입자에 비하여 크게 증가하였다. 알루미나를 분산시켜 소결한 {{{{ { Al}_{2 }{ O}_{3 } }}}}/Ce- TZP 시편의 경우, 알루미나 입자에 의해서 입성장이 크게 억제되었고, 세리아를 도핑한 후 소결과 열처리를 행한 {{{{ { Al}_{2 }{ O}_{3 } }}}}/Ce-TZP에서는 불규칙 입자형상이 형성되면서도 입성장이 억제되어 입자크기에 비하여 입계면적이 크게 증가하였다. 분산된 알루미나 입자들은 소결과 열처리 과정 중 입자크기가 증가하였고, 열처리 동안 많은 입자들이 입계에서 입내로 위치가 변화하였다. 정상적인 입자형성을 갖은 시편에서는 균열진전시 입계파괴가 주로 일어났으나 불규칙 입자형성을 갖는 시편에서는 주로 입내파괴가 관찰되었다.

• 수산해양기술연구
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• 제11권1호
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• pp.15-20
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• 1975

The catch per unit effort and the migration of Alaska pollack Theragra chalcogramma were deduced from the catch data of 1, 208 hauls of a stern trawler operated on and around the continental shelf east of the Kamchatka Peninsula and Kurile Islands from October 1974 to December 1975. Alaska pollack occupied more than ninety per cent of the total catch of approximately 17, 760 metric tons, while the cuttle and flat fishes occupied less than five percent. Beside those fishes, approximately 131 metric tons of Alaska pollack roe were produced during the voyage from February to April 1975. The mean catch per unit effort in each sub-area of fishing ground was approximately 15.5 metric tons and the peak of the CPUE occured in January and August. The CPUE in the continental shelf east of Kamchatka Peninsula ($50^{\circ}- 52^{\circ} N$ Lat) showed the maximum of 25-34 metric tons in August. The main fish school is thought to migrate southward speedily in August-September along the continental shelf from the Kamchatka Peninsula to the east of Kurile Islands. The CPUE on the continental shelf east of Kamchatka Peninsula was higher than that on the steeper continental slope. However, the CPUE on the steeper flank of the bank east of Paramushiru Island and Onekotan Island was remarkably higher than that on the relatively flat top of the bank. The small-sized fishes of less than thirty centimeter in fork length began to recruit in the fishing ground since August 1965.

• Fisheries and Aquatic Sciences
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• 제25권5호
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• pp.298-310
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• 2022

Although giant and large fish species are highly important as a keystone species in the marine ecosystem, there have been no or few studies on their spatio-temporal variations around the Korean peninsula. For this, we analyzed daily reports made by observers at 57 fishery landing sites in Korea over 6 years, from 2011 to 2016. In total, 153 fish species were re-identified based on photos and descriptions recorded by Korean observers, of which five species were classified as a giant fish over 5 m in maximum total length (MTL) and 17 species as a large fish from 3 m to 5 m MTL according to the data presented by Froese & Pauly (2021). Among the giant and large fish species, Mola mola was the most abundant species, with 75 individuals landed as by-catch. The second most abundant species was Isurus oxyrinchus (31), followed by Mobula mobular (23), Lamna ditropis (17), Masturus lanceolatus (16), Sphyrna zygaena (14), and Prionace glauca (12). As a result of cluster analysis based on the number of individuals of giant and large fish species by year and sea, six years were separated into two clusters (2011-2013 vs. 2014-2016), with high contribution of M. mola, I. oxyrinchus, and M. lanceolatus; and three seas were separated into two clusters (eastern + southern seas vs. western sea), with high contribution of M. lanceolatus, L. ditropis, and I. oxyrinchus. The largest number of M. mola accounted for 64% of the total in 2014 and 2016, and 71% in summer (June-August). It is assumed to have a correlation between seawater temperature fluctuation and the occurrence of giant and large fish species. Our study highlights importance of longterm monitoring of giant and large fish species, and can help to understand the life cycle such as natal or nursery migration of giant and large fish species around the Korean peninsula.

• 한국수자원학회논문집
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• 제37권7호
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• pp.541-552
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• 2004

본 연구에서는 설치어도조사법을 적용하여 한강 하류부 잠실수중보의 회유성 어류 이동을 위해 설치된 계단식 어도에 대해 어류소상기능을 조사하고 피 개선방안을 제시하였다. 조사기간 동안에 어도 출구에서 채집된 어도이용 소상어류는 체장이 29cm 이상인 강준치가 361개체로서 대부분을 차지하였으며, 그 강준치에 대한 소상능력은 최대 2.53${\times}$10^{-3}$ 개체/hr/g으로 나타났다. 잠실수중보의 계단식 어도는 한강에 서식하는 어류의 다양성을 만족시키기 어려운 형태일 뿐만 아니라 어도 출구의 월류격벽 낙차가 너무 크고 과도한 유량이 유입되기 때문에 유영력이 약한 어류가 이용할 수 없다. 따라서 이 어도는 유영력이 큰 강준치와 누치 이외의 다른 어류에 대해서는 소상기능을 발휘하지 못하고 있는 것으로 확인되었다. 어도의 기능을 향상시키기 위해서는 다양한 어류가 이용할 수 있는 형식으로 어도 구조를 변경하고, 어도 시설의 기능을 고려하여 가동보를 운영하여야 한다. 또한 고정보 전체의 상시 월류로 인해서 생기는 어도의 설치효과 저하에 대해서는 저수로 양안 측에 어도를 추가 설치할 필요가 있는 것으로 판단된다.