• Journal of Plant Biotechnology
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• 제6권4호
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• pp.265-268
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• 2004

Cell growth and ginseng saponin production by large-scale suspension (bioreactor) cultures of Panax ginseng were investigated under various inoculum sizes. Cell growth was low at an inoculum size of 40 g FW/L, and the maximum cell growth was obtained with increasing inoculum size up to 100 g FW/L. The cell density of 333 g FW/L and 12.7 g DW/L was obtained at inoculum size of 100 g FW/L after 30 days of cultivation. Maximum saponin production of $4.40\;\cal{mg/g}$ DW was achieved at 60 g FW/L of inoculum size. Thus, inoculum size 60 g FW/L was suitable for optimum biomass accumulation as well as saponin production during bioreactor cultivation of ginseng suspension cells.

• 대한전자공학회논문지
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• 제24권6호
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• pp.1074-1079
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• 1987

We present a fast, constructive algorithm for the automatic placement of standard cells, which consists of two steps. The first step is responsible for cell-row assignment of each cell, and converts the circuit connectivity into a multi-stage graph under to constraint that sum of the cell-widths in each stage of the multi-state graph does not exceed maximum cell-row width. Generatin of feed-through cells in the final layout was shown to be drastically reduced by this step. In the second step, the position of each cell within the row is determined one by one from left to right so that the cost function such as the local channel density is minimized. Our experimental result shows that this algorithm yields near optimal results in terms of the number of feed-through cells and the horizontal tracks, while running about 100 times faster than other iterative procedures such as pairwise interchange and generalized force directed relaxation method.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.131-135
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• 2005

Under sulfur deprived conditions, PS II and photosynthetic $O_2$ evolution by Chlamydomonas reinhardtii UTEX 90 are inactivated, resulting in shift from aerobic to anaerobic condition. This is followed by hydrogen production catalyzed by hydrogenase. We hypothesized that the photosynthetic capacity and the accumulation of endogenous substrates such as starch for hydrogen production might be different according to cell age. Accordingly, we investigated (a) the relationships between hydrogen production, induction time of sulfur deprivation, increase of chlorophyll after sulfur deprivation, and residual PS II activity, and (b) the effect of initial cell density upon sulfur deprivation. The maximum production volume of hydrogen was 151 ml $H_2$/l with 0.91 g/l of cell density in the late-exponential phase. We suggest that the effects of induction time and initial cell density at sulfur deprivation on hydrogen production, up to an optimal concentration, are due to an increase of chlorophyll under sulfur deprivation.

• 한국통신학회논문지
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• 제33권10B호
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• pp.911-918
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• 2008

본 논문에서는 방향성 안테나를 이용하여 3섹터 구조를 갖는 Narrow-Beam Trisector Cell (NBTC)과 Wide-Beam Trisector Cell (WBTC) 구조의 WiBro 시스템에 IEEE802.16j 기반의 멀티홉 중계기를 도입하여 커버리지를 확장하는 새로운 형태의 NBTC와 WBTC 구조를 제안한다. 제안한 구조는 최적화 모델을 통해 트래픽 분포에 따른 최적 배치된 기지국과 멀티홉 중계기의 수, 각 기지국의 확장되는 커버리지, 그리고 설치비용 등을 기지국만 배치하는 기존의 전형적인 구조와 비교 분석하였다. 분석 결과를 통해 주어진 전체면적에 중계기를 효율적으로 배치한다면 절약되는 설치비용을 알 수 있었고, 제안하는 멀티홉 중계기가 도입된 NBTC와 WBTC 방식 중에서 트래픽 분포가 높은 지역에서는 WBTC 방법을 이용하고, 트래픽 분포가 적은 지역에서는 NBTC 방법을 이용한다면 커버리지 확장과 설치비용 절약 측면에서 보다 효율적임을 알 수 있었다.

• Journal of Information Display
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• 제9권3호
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• pp.33-38
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• 2008

A simple ray tracing model is developed to establish the relationship between the distributions of observed visible light and the excited Xe species in a PDP cell. The ray tracing model can obtain the density distribution of the excited Xe species. It shows the two independent discharge modes created during a single period of sustaining discharge. The maximum density of excited Xe species is located about $148{\mu}m$ away from the center of the gap between two sustaining electrodes. We also found the loss rate of VUV photons by comparing the luminance profile predicted in our theoretical model from experimental results. According to calculations based on our model, only about 22 percent of the photons can impinge the phosphor layer.

• 한국수소및신에너지학회논문집
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• 제19권6호
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• pp.498-504
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• 2008

Metal-reducing bacterium, Geobacter sulfurreducens is available for mediator-less microbial fuel cell (MFC) because it has biological nanowires(pili) which transfer electrons to outside the cell. In this study, in the anode chamber of the MFC system using G. sulfurreducens, the concentrations of NaCl, sodium phosphate and sodium bicarbonate as electrolytes were mainly optimized for the generation of electricity from acetate. 0.4%(w/v) NaClO and 0.5M $H_2SO_4$ could be utilized for the sterilization of acrylic plates and proton exchange membrane (major construction materials of the MFC reactor), respectively. When NaCl concentration in anode phosphate buffer increased from 5 to 50 mM, power density increased from 6 to $20\;mW/m^2$. However, with increasing sodium phosphate buffer concentration from 5 to 50 mM, power density significantly decreased from 18 to $1\;mW/m^2$. Twenty-four mM sodium bicarbonate did not affect electricity generation as well as pH under 50 mM phosphate buffer condition. Optimized anode chamber of MFC using G. sulfurreducens generated relatively high power density ($20\;mW/m^2$) with the maximum coulombic efficiency (41.3%).

• 생태와환경
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• 제34권3호통권95호
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• pp.175-184
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• 2001

방류수내 남조류 세포의 밀포와 종조성 및 독소의 계절별, 일일 변화를 일본 중심에 위치하고 있는 Suwa호의 수문에서 1998년 5월부터 10월까지 조사하였다. Microcystis의 높은 세포밀도가 M. ichthyoblabe가 우점했던 7월과 M. viridis가 우점종으로 나타난 9월에 관찰되었다. Microcystis의 종조성과 총 세포밀도는 3종류의 microcystin의 정성${\cdot}$정량적인 변화와 관련이 있었다. M. ichthyoblabe가 우점했던 7월 동안에는 MC-RR과MC-LR만이 검출되었으나 M. aeruginosa의 개체수 증가와 함께 M. viridis가 우점종으로 나타난 8월부터 10월까지는 MC-YR을 포함한 3종류의 microcystin 모두 검출되었다. 방류수내 Microcystis의 세포밀포와 microcystin의 함량의 일 변화는 바람의 일 변화에 큰 영향을 받았다. 아침에 비해 오후에 바람이 강하게 부는 풍속주기의 조건에서 Microcystis의 세포밀도와 독소의 함량은 아침에 증가하고 오후에 감소하는 경향을 나타냈다. 본 연구의 결과들은 수문이나 취수탑에서 방류시기의 조절은 부영양 호수에서 물을 취수하여 이용함에 있어 원수내 조류의 생물량을 저감할 수 있는 유효한 방법임을 제시한다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권1호
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• pp.72-74
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• 2001

Transgenic Nicotiana tabacum cells were cultivated for the production of murine granulocyte macrophage-colony stimulating factor (mGM-CSF) in both a stirred tank bioreactor and an airlift bioreactor with draft tube. Cell growth and mGM-CSF production in the airlift bioreactor were found to be better than those achieved in the stirred tank bioreactor. In the airlift bioreactor, 9.0g/L of cells and 2.2ng/mL of mGM-CSF were obtained (11.0g/L and 2.4ng/mL, respectively in shake flasks). Although the lag period was prolonged and mGM-CSF production was lowered by 33% in the stirred thank bioreactor as compared to the control culture, the maximum cell density was increased up to 12.0g/L due to better mixing by agitation at the higher cell density.

• Journal of Information Display
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• 제11권4호
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• pp.149-153
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• 2010

To improve the luminescence characteristics of high-efficiency alternating-current plasma display panels (AC-PDPs), we developed a new hoof-type electrode structure, and we studied the spatiotemporal behavior of the density of the excited Xenon atom in the $1s_5$ metastable state via laser absorption spectroscopy. Using this structure, the maximum density of the excited Xenon atom per cell was improved by 2.4 times that when the conventional electrode structure was used.

• Nuclear Engineering and Technology
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• 제55권2호
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• pp.749-755
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• 2023

A new approximation method was proposed for treating the non-resonant spatial self-shielding effects of double heterogeneous region such as the double heterogeneous effect of VHTR fuel compact in the thermal energy range and that of BP compact with BISO. The method was developed based on the effective homogenization method and a spherical unit cell model with explicit coated layers and a matrix layer. The self-shielding factor was derived from the relation between the collision probabilities for a double heterogeneous compact and the effective cross section for the homogenized compact. First, the collision probabilities and transmission probabilities for all layers of the spherical model were calculated using conventional collision probability solver. Then, the effective cross section for the homogenized sphere cell representing the homogenized compact was obtained from the transmission probability calculated using the probability density function of a chord length. The verification calculations revealed that the proposed method can predict the self-shielding factor with a maximum error of 2.3% and the double heterogeneous effect with a maximum error of 200 pcm in the typical VHTR problems with various packing fractions and BP compact sizes.