Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Inoculum Size on Biomass Accumulation and Ginsenoside Production by Large-Scale Cell Suspension Cultures of Panax ginseng

  • Thanh Nguyen Trung (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Murthy Hosakatte Niranjana (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Department of Botany, Karnatak University) ;
  • Yu Kee-Won (CBN Biotech Co. Ltd.) ;
  • Jeong Cheol Seung (CBN Biotech Co. Ltd.) ;
  • Hahn Eun-Joo (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Paek Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
  • Published : 2004.12.01
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Abstract

Cell growth and ginseng saponin production by large-scale suspension (bioreactor) cultures of Panax ginseng were investigated under various inoculum sizes. Cell growth was low at an inoculum size of 40 g FW/L, and the maximum cell growth was obtained with increasing inoculum size up to 100 g FW/L. The cell density of 333 g FW/L and 12.7 g DW/L was obtained at inoculum size of 100 g FW/L after 30 days of cultivation. Maximum saponin production of $4.40\;\cal{mg/g}$ DW was achieved at 60 g FW/L of inoculum size. Thus, inoculum size 60 g FW/L was suitable for optimum biomass accumulation as well as saponin production during bioreactor cultivation of ginseng suspension cells.

Keywords

References

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