• Journal of Plant Biotechnology
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• v.6 no.4
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• pp.265-268
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• 2004

Cell growth and ginseng saponin production by large-scale suspension (bioreactor) cultures of Panax ginseng were investigated under various inoculum sizes. Cell growth was low at an inoculum size of 40 g FW/L, and the maximum cell growth was obtained with increasing inoculum size up to 100 g FW/L. The cell density of 333 g FW/L and 12.7 g DW/L was obtained at inoculum size of 100 g FW/L after 30 days of cultivation. Maximum saponin production of $4.40\;\cal{mg/g}$ DW was achieved at 60 g FW/L of inoculum size. Thus, inoculum size 60 g FW/L was suitable for optimum biomass accumulation as well as saponin production during bioreactor cultivation of ginseng suspension cells.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.250-253
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• 2000

We researched effects of growth on initial inoculum size, liquid volume, and medium feeding rate etc. Cell suspension inoculated at low cell concentrations showed a typical growth reduction, whereas root cultures showed an improvement in growth. In this paper, Hairy roots showed high growth rate at 0.4 % inoculum size and 100 mL liquid volume in 250 mL flask cultures.

• KSBB Journal
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• v.16 no.4
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• pp.376-380
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• 2001

The human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was produced from cell suspension culture of transgenic tobacco which was transformed by using Agrobacterium harboring the hGM-CSF gene. To improve the production of hGM-CSF in batch culture system, the effects of initial sucrose concentration and inoculum size were investigated. The results show that the hGM-CSF production was not affected by small inoculum size in medium containing either low or high concentration of sucrose. However, the production of hGM-CSF was increased under increasing of the inoculum sizes and sucrose concentration. Under the combination of inoculum and sucrose concentration, the maximum hGM-CSF production of 720 $\mu$g/L was obtained at 90 g/L of initial sucrose concentration and 110 g/L of inoculum size.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.5
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• pp.377-381
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• 2006

A study was carried out to determine a suitable light intensity and inoculum size for the growth of Rhodopseudomonas palustris strain B1. The pollution reduction of sago effluent using free and immobilised R. palustris cells was also evaluated. The growth rate in glutamatemalate medium was highest at 4 klux compared to 2.5 and 3 klux. The optimal inoculum size was 10% (v/v). Both the COD and BOD of the sago effluent were reduced by 67% after three days of treatment. The difference in biomass production or BOD and COD removal with higher inoculum sizes of 15 and 20% was minimal. This could be attributed to limited nutrient availability in the substrate. The use of immobilised cells of R. palustris reduced the pollution load 10% less compared to pollution reduction by free cells. Hence, there was no significant difference in using free or immobilised cells for the treatment of sago effluent.

• Journal of Life Science
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• v.13 no.5
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• pp.705-711
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• 2003

The gellan was produced by Pseudomonas elodea under aerobic condition. In this study, the effects of inoculum size, carbon sources and concentration, nitrogen source, and C/N ratio on the cell growth and the production of gellan were evaluated. The maximum growth of P. elodea and gellan production was obtained at 5% (v/v) of inoculum size and glucose showed best results among 9 carbon sources tested. The maximum specific yield of 2.22 and productivity of $0.03 g/\ell$h were obtained at 1.0% (w/v) of glucose. The maximum gellan production was obtained at medium without ammonium nitrate. This indicates that nitrogen limitation is essential for the production of gellan. The highest cell and gellan production were obtained at 20 of C/N ratio.

• Journal of Life Science
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• v.6 no.2
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• pp.94-103
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• 1996

To understand effect of inoculum size, cell density, sucrose concentration and concentrations of MS basal on suspension culture and protoplast isolation of wild viola(Viola patrinii DC.) callus from petiole segments this experiment was conducted. In the lot of 30 mesh inoculum size, two observations were; One was that a considerable increase in the fresh and dry weight of callus was determined. Another was that the callus mass was relatively compact compared with others. A recommendable cell density was 0.4g for 20ml culture medium and the higher sucrose concentration, the higher fresh and dry weight were obtained. The dilution of MS basal salt was differently affected on fresh and dry weight; the highest fresh weight was found in 1x MS salt, while the higest dry weight was in 1/3x dilution.The addition of casein hydrolysate(3g/L) was more effective to increase of both fresh and dry weight. THe contents of protein was great in the inoculum lots with larger inoculum sizeand higher concentration of MS basal salts contenting 3g/L of casein hydrolysate and higher sucrose compared with others. The greatest protoplasts were isolated from the lot of 10 mesh size treated with 1%pectinase SE-150 and 2% cellulase YC. In general, for optimal protoplast isolation the followingconditions were recommended; 1) Use of smaller cell size cultured for 2-5 weeks, and 2) more than 5 hours incubation using the combined mixture of the enzymes with proper concentrations.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.203-206
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• 1992

Conditions of fermentation for the production of demethyltetracycline were examined using the mutant, which was obtained through the cell fusion of demeclocycline producing strains, The optimum temperature and the initial pH of broth for demethyltetracycline fermentation were $25^{\circ}C$ and 6.7, respectively. Unlike any other cases, the control of pH with alkali solution during the fermentation process affected the productivity. As a general rule, the larger the inoculum size the higher the early consumption of sugar and the viscosity of broth, which means that fermentation proceeds more rapidly as the inoculum size is increased. The highest productivity was shown when the inoculum size was 5% (v/v), and the phase of seed also influenced the fermentation. Among the parameters of pre-culture thus examined, pH was the most important factor.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.1
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• pp.21-25
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• 2002

To maximize and sustain the productivity of Monascus pigments, various environmental and nutritional parameters, such as the initial moisture content, pH, inoculum size, sample size, and nutrient supplement, that influence pigment production were evaluated in solid-state cultures as follows: initial moisture content, $50\%;$ pH, 6.0; inoculum size $1\;\times\;10^4$ spore cells $(grams\;of\;dry\;solid\;substrate)^{-1};$ sample size, 300 g. All supplementary nutrients (carbon, nitrogen, and mineral sources) added has inhibitory effects on the cell growth and red pigment production. In open tray culture the maximum biomass yield and specific productivity of red pigments were 223 mg DCW $(grams\;of\;initial\;dry\;substrate)^{-1}$ and, $47.6\;OD_{500}\;(DCW\;grams)^{-1}h^h{-1}$ respectively.

• Korean journal of applied entomology
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• v.14 no.1 s.22
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• pp.1-5
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• 1975

The study has been carried out for the development of an inoculation method in screening resistant varieties and/or lines to bacterial leaf blight of rice with special consideration on plant ages and inoculum concentrations. A higher incidence of the disease was found on younger plants than on the older ones when the plants were inoculated with the same concentration of inoculum by clipping method under the same circumstances. Applications of extremly high concentration of inoculum resulted the death of younger seedlings from all varieties within short period after inoculation, and the inoculum that was lower than optimum concentration failed in distinguishing varietal characteristics in regard of resistance. The proper inoculum size for the screening of resistance among the varieties and/or lines depended on the age of plants. The optimum concentration of inoculum for the plant age of 14, 37, 48 and 58days was $10^6,\;10^7,\;10^7-10^{-9}\;and\; 10^9 cells/ml.,$ respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.2
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• pp.177-181
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• 1985

Aflatoxin production by Aspergillus parasiticus NRRL 2999 with two different levels of inoculum sizes ($10^2$ and $10^6$ spores) was studied on such varieties of soybeans produced in Korea as Bongeui, Hwang Keum, Jangbaek, Danyeop, Jangyeop and Namcheon. With the inoculum size of $10^2$ spores/10 g of soybeans, there were statistically difference (p<0.05) on the production of aflatoxins in the varieties of soybeans. The variety of Namcheon produced 7 times higher amount of aflatoxin $B_1$ than that of Bongeui. The variety of Jangyeop produced the highest level of aflatoxin $G_1$ among the varieties employed. Namcheon and Jangyeop were the varieties which supported high amounts of aflatoxins. Much higher levels of aflatoxins were obtained on the soybeans than on rice in general with the inoculum size of $10^2$ spores. With the inoculum size of $10^6$ spores/10 g of soybeans, significantly lower levels of aflatoxins were synthesized by the mold than those with $10^2$ spores. The variety of Namcheon resulted in the lowest production of aflatoxin $B_1$ while Hwangkeum was the variety that produced highest amount of aflatoxin $B_1$. There were no significant differences within the varieties on the production of aflatoxin $G_1$ by the mold except the variety of Hwangkeum. Lower quantities of aflatoxin $B_1$ were produced by the mold en the soybeans than on rice with the inoculum size of $10^6$ spores.