• KSBB Journal
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• 제11권3호
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• pp.303-310
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• 1996

해양 미세조류인 Isochrysis galbana Parke의 성장에 미치는 여러 환경요인과 배지요인의 영향을 조사하고 최적 배양조건을 구하였다. 그 결과를 요약하 면 다음과 같다. 1. 인공바닷물은 자연바닷물보다 다소 낮은 비성 장속도와 최종 균체농도를 나타내었다. 2.. 자연바닷물이 기초배지로 사용되었을 때 2mM 이상의 충분한 질산염 농도가 필요하였다. 그러나 미량원소와 비타민의 영향은 관찰되지 않았다. 3. 완충용액요로 Tris를 첨가한 경우 pH는 일정 하게 유지가 가능하나 비성장속도와 최종 균체농도 가 감소하였다. 4. 최적 초기 pH와 온도는 각각 pH 8, $20^{\circ}C$ 였다. 5. 벚세기를 400-21001ux의 범위에셔 변화시켰을 때 비성장속도는 18501ux까지 증가하였고 그 이 후로는 일정하였다. 6. 10mL에서 70mL로 Working volume을 증가 시켰을 때 초기 성장속도는 비슷하였으나 최종 균체 농도가 감소하는 경향을 보였다. 7. Working volume이 30mL일 때 최적 교반속 도는 100rpm정도이고, 더 높은 교반속도에서는 최 종 균체농도의 감소가 관찰되었다. 8. 플라스크배양의 최적조건에서 최대 비성장속도 와 최종 균체농도는 각각 $O.021hr^{-1}$과 1.1g/L로 얻어졌다.

• Journal of Advanced Marine Engineering and Technology
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• 제35권4호
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• pp.414-420
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• 2011

평균전류밀도 0~2000 $A/m^2$ 의 운전범위에 대한 음극 지지형 고체산화물 연료전지의 단위셀에 대한 열응력해석을 수행하였다. 평균전류밀도가 2000 $A/m^2$ 운전에서, 단위전지 열유동에 대한 수치해석적 방법으로 얻어진 온도분포를 토대로 구조해석을 수행하였다. 온도 편차가 매우 미미한 상태 에서 이러한 유체-구조 연성 해석 방법을 통하여 완전 결합된 조건에서 최대등가응력이 전해질은 262.58MPa, 캐소드는 28.55MPa, 애노드는 15.1MPa로 계산되어 전해질에서 가장 높은 응력이 발생함 을 알 수 있었다. 또한, 마찰접합조건인 경우 마찰계수가 증가함에 따라 응력이 증가함을 알 수 있었으며, 이는 셀 내부 물질간의 결합력에 의한 응력이 지배적임을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.967-974
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• 2012

Cultivation of Spirulina platensis using ammonium salts or wastewater containing ammonium as alternative nitrogen sources is considered as a commercial way to reduce the production cost. In this research, by analyzing the relationship between biomass production and ammonium-N consumption in the fed-batch culture of Spirulina platensis using ammonium bicarbonate as a nitrogen nutrient source, an online adaptive control strategy based on optical density (OD) measurements for controlling ammonium feeding was presented. The ammonium concentration was successfully controlled between the cell growth inhibitory and limiting concentrations using this OD-based feedback feeding method. As a result, the maximum biomass concentration (2.98 g/l), productivity (0.237 g/l d), nitrogen-to-cell conversion factor (7.32 gX/gN), and contents of protein (64.1%) and chlorophyll (13.4mg/g) obtained by using the OD-based feedback feeding method were higher than those using the constant and variable feeding methods. The OD-based feedback feeding method could be recognized as an applicable way to control ammonium feeding and a benefit for Spirulina platensis cultivations.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.173-181
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• 2004

To delay the onset of apoptosis in the culture, transformed Tn 5B1-4 cells harboring anti-apoptotic genes, bcl-2 and baculovirus p35, have been established and analyzed for their anti-apoptotic ability in suspension culture using spinner flasks. In the suspension culture at agitation speeds of 100 rpm and 200 rpm, the cell growth of cell clone expressing Bcl-2 protein was much higher than other two clones and the maximum cell density of the clone was 6.0 ${\times}$ 10$^{6}$ cells/ml and 6.2 ${\times}$ 10$^{6}$ cells/ml at day three of the incubation. On the other hand, the cell growth of cell clone expressing baculovirus protein P35 was much higher than other two clones in suspension culture at agitation speed of 300 rpm and the maximum cell density of the clone was 6.1 ${\times}$ 10$^{6}$ cells/ml at day three of the incubation. Based on the pattern of genomic DNA laddering and the microscopic observation of apoptotic bodies, the more apoptotic bodies are induced in Tn 5B1-4 control cell clone at higher agitation speed. This result shows that the shear stress can be a main factor in inducing apoptosis in spinner flask culture. At low agitation speed, cell clone expressing Bcl-2 was more effective in delaying the onset of apoptosis than the cell clone expressing P35. On the other hand, at high agitation speed, cell clones expressing baculovirus P35 was more effective in delaying the onset of apoptosis than the cell clone expressing Bcl-2. Therefore, anti-apoptotic genes, bcl-2 and baculovirus p35, can playa distinct role depending on agitation speed in the suspension culture.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.545-548
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• 2001

Microbiologically influenced corrosion (MIC) of metal is common in the natural environment and sulfate reducing bacteria are representative microorganisms for MIC. We found that biofilm fomlation by SRB on the metal surface might be controlled by quorum sensing, which is a cell density dependent regulation of cell metabolism. As cell free culture fluids (spent media) of Desulfovibrio vulgaris and D. desulfuricans were tested for quontrn sensing related test strains, it was found that spent media of two SRB induced increased luminescence of Vibrio harveyi BB886 (sensor 1+, sensor 2-) and BB170 (sensor 1-, sensor 2+). Quorum activities of D. vulgaris and D. desulfuricans appeared to be parallel to growth patterns, i.e., it was low in the lag phase, highly increased in the exponential phase, and reached maximum in the stationary phase. Interestingly, however, luminescence of V. harveyi BB886 and BB170 induced by a unit cell mass of the SHB showed a maximal peak in the late lag phase. Hence, it was suspected that quorum sensing of these two SHB play unknown roles in shifting cells from dormant to growth stages.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.312-315
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• 2005

A process for efficient recycle fed-batch culture was carried out to increase cell mass and spore production by Lactic acid bacteria isolated from Kimchi. A large quantity of cell mass obtained by feeding concentration of sugar in recycle fed-batch culture. When the high density of salt was created that the cell mass was come-down. In this study, cultured in different feeding concentration of sugar conditions. Lactic acid bacteria by recycle fed-batch culture was investigated in 2L working volume of fermenter, obtained the maximum cell mass was 15.17g/L.

• Environmental Engineering Research
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• 제22권2호
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• pp.157-161
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• 2017

Simultaneous treatment of food waste leachate and power generation was investigated in an air-cathode microbial fuel cell. A TCOD removal efficiency of $95.4{\pm}0.3%$ was achieved for an initial COD concentration of 2,860 mg/L. Maximum power density ranged was maximized at $1.86W/m^3$, when COD concentration varied between 60 mg/L and 2,860 mg/L. Meanwhile, columbic efficiency was determined between 1.76% and 11.07% for different COD concentrations. Cyclic voltammetric data revealed that the oxidation peak voltage occurred at -0.20 V, shifted to about -0.25 V. Moreover, a reduction peak voltage at -0.45 V appeared when organic matters were exhausted, indicating that reducible matters were produced during the decomposition of organic matters. The results showed that it was feasible to use food waste leachate as a fuel for power generation in a microbial fuel cell, and the treatment efficiency of the wastewater was satisfied.

• KSBB Journal
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• 제10권4호
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• pp.401-405
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• 1995

S. platensis의 옥외연속 대량배양을 위해 125P 배양조를 설계, 제작하여 배양조건에 따른 생육 변수를 검토하였다. 0.30(1/day)의 비생육 속도와 1.69(g/$\ell$) 의 최대 세포농도가 회분배양에셔 얻어졌 으며 유가삭 배양에셔 비생육 속도와 최대 세포농도 는 각각 0.22(1/day)와 1.75(g/$\ell$) 였다. 연속 배양 에서 얻어진 상.하층 배양조의 최대 균처l생산생은 각각 0.44와 0.43(g/$\ell/day$) 였다. 따라서 인위적으로 온도, 광도 등의 생육조건을 고려하여 S. platensis를 연속 배양할 경우 경제성이 클 것으로 사려된다. 아울라 top-driven stirrer와 air sparging 장치를 이용하여 교반 효과를 비교한 결과 top­d driven stirrer를 이용한 배양조의 균채 생산성이 다소 높은 갯으로 냐타났다.

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1024-1030
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• 2001

The key factors for high-density Haematococcus pluvialis cultures and conditions for astaxanthin induction were examined to maximize astaxanthin production. Light intensity was found to be the most important factor, and thus experiments were found to be the most important factor, and thus experiments were carried out using different light sources and intensities. A high cell density of over 2.7 g/l was obtained at $75{\mu}E/m^2/s$, whereas a much lower cell concentration (<1.0 g/ 1) was obtained with lower light intensities $(15-30{\mu}E/m^2/s$. A high light intensity and the supplement of 470 nm photons had a more dramatic effect on the final astaxanthin concentration and per cell astaxanthin content. A maximum astaxanthin concentration of 6.5 mg/l was obtained at a light intensity of $160{\mu}E/m^2/s$, whereas only 1.3 and 0.7 mg/l were obtained at 30 and $15{\mu}E/m^2/s$, respectively. A supplement of 470 nm photons enhanced the carotenoid and chlorophyll formation.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.36-39
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• 2013

We introduce a high-performance microbial fuel cell (MFC) that was operated using a 0.1M bicarbonate buffer as the cathodic electrolyte. The MFC had a 136.42 $mW/m^2$ maximum power density under continuous feeding of 5 mM acetate as fuel. Results of the electrode potential measurements showed that the cathode potential of the bicarbonate-buffered condition was higher than the phosphate-buffered condition, although the phosphate condition had less interfacial resistance between the membrane and electrolyte. Therefore, we posit here that the increased power of the bicarbonate-buffered MFC may be caused by the higher cathode potential rather than by the interfacial membrane-electrolyte resistance.