• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 심포지엄
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• pp.9-18
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot idtiation from nodal segments was very rare. Mature apices produced callus. Although removed of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.

• Journal of Forest and Environmental Science
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• 제38권3호
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• pp.174-183
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• 2022

The study aims to examine population indices of mature trees in Jebel Elgarrie forest, Blue Nile State, Sudan. We used remote sensing techniques to stratify the forest into vegetation classes depending on tree density. We distributed 97 circular sample plots (0.1 ha) proportionally to the area of the vegetation classes. In each sample plot we identified, counted and recorded all mature trees (DBH ≥10 cm). We calculated frequency, density, abundance, richness, evenness and diversity for each species and we drew abundance rank curve of mature trees. We used One-Way ANOVA to test for differences (α=0.05) in mean density (No./ha) of mature trees between vegetation classes. Results revealed that the forest was conveniently sub-divided into high density (C1), medium density (C2), low density (C3) and bare farm land (C4) classes. We identified fifteen tree species that belong to 10 families and 14 genera. Combretaceae and Fabaceae were the common families while Anogeissus leiocarpa was the most frequently occurring species. While species diversity varied between vegetation classes, diversity of the forest as a whole is low. While mean density of mature trees in C1, C2, C3 and C4 it was 100, 74, 10, and 0, respectively, it was 54 for the whole forest indicating low stocking, Following One-Way ANOVA, multiple comparisons revealed significant differences in mean density of mature trees between C1 & C3 and C2 & C3. The study provided empirical results on population indices of mature tree species, which would be of importance for successful management and conservation of the forest.

• 한국작물학회지
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• 제33권3호
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• pp.209-214
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• 1988

질소비료의 증비와 숙도의 차이가 버어리종 잎담배의 수량과 품질 및 내용성분에 미치는 영향을 시험한 결과는 다음과 같다. 1. 질소비료가 증가될수록 건조엽의 적색도가 증가되었고, 2. 엽분별 수확시기를 보면 하위엽은 미숙엽에서, 상위엽은 과숙엽에서 수확할 때 kg당 가격이 가장 낮았다. 3. 미숙엽과 과숙엽은 수량이 대등하였으나 과숙엽은 수량이 떨어졌다. 4. 질소비료의 증비와 숙도에 따른 단백질 pattern의 차이는 나타나지 않았다. 5. 생육후기에는 비료수분 및 숙도에 따른 Nitrate reductase 활성도에는 차이가 나타나지 않았다.

• 산업융합연구
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• 제20권12호
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• pp.251-259
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• 2022

최근 평생학습 요구 증가와 간호직의 높은 취업률로 간호학과를 지원하는 만학도의 수가 증가하고 있다. 본 연구는 간호학과 만학도 학생들의 자기효능감, 전공만족도, 대학생활 적응을 확인하고, 대학생활 적응에 영향을 미치는 요인을 파악하기 위해 시도되었다. 연구대상은 30세 이상 간호 대학생 148명으로, 2021년 10월부터 12월까지 자료 수집을 실시하였으며, 수집된 자료는 IBM SPSS 25.0 프로그램을 이용하여 분석하였다. 분석 결과 자기효능감은 3.69±0.51점, 전공 만족도는 4.07±0.50점, 대학생활 적응은 3.42±0.50점으로 나타났다. 간호학과 만학도 학생들의 대학생활 적응에 학년(β=.20, p=.001)과 자기효능감(β=.43, p<.001) 그리고 전공만족도(β=.37, p<.001)가 유의한 영향이 있었으며, 설명력은 54%였다. 간호학과 만학도 학생들은 앞으로 더 증가할 것으로 예측되며, 이상의 연구 결과를 바탕으로 자기효능감과 전공만족도를 높일 수 있는 중재 프로그램을 제공하여 간호학과 만학도 학생들의 대학생활 적응을 향상시킬 것을 제언한다.

• Clinical and Experimental Reproductive Medicine
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• 제27권2호
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• pp.191-200
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• 2000

Objective: This study was carried out to compare the effects of the stepwise exposure treatments on the morphological normality, fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing and to use as a fundamental data for the cryopreservation of human oocytes. Materials and Methods: The morphological normality and fertilization rates of the vitrified and ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were observed. After choosing the 3step exposure treatment groups, we observed the morphological normality and fertilization, blastocyst formation rate of the vitrified and ultra-rapid frozen mouse mature oocytes. Results: The morphological normality and fertilization rates of the vitrified mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 75%, 85%, 88% and 58%, 61 %, 54% respectively. There were no significant differences among treatments(p>0.05). The morphological normality and fertilization rate of the control was 92% and 65%. There were no significant differences in fertilization rate among control and treatments (p>0.05). The morphological normality and fertilization rates of the ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 83%, 83%, 84% and 75%, 63%, 56% respectively. There were no significant differences among treatments (p>0.05). The morphological normality and fertilization rate of the control was 95% and 67%. There were no significant differences among control and treatments (p>0.05). The morphological normality and fertilization rate of the vitrified or ultra-rapid frozen mouse mature oocytes after 3step exposure treatment were 69% and 75%, respectively. The blastocyst formation rate was 60% and 57%. The results did not differ significantly between vitrification and ultra-rapid freezing (p>0.05). Conclusion: As known in the above results, there were no significant differences in the fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing among the control and treatments. It is suggested that vitrification and ultra-rapid freezing method were effective for the cryopreservation of mouse mature oocytes.

• 한국경제지리학회지
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• 제8권1호
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• pp.1-16
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• 2005

클러스터에 대한 기존 논의는 성숙산업지역과 첨단산업지역의 차이를 크게 부각시켰으며 성숙산업지역은 혁신과 기업가정신이 미약하고 경쟁력이 약하여 혁신지역으로 변모하기 어렵다는 평가를 한다. 하지만 지역의 사회적 자본, 국지적 네트워크형성, 신뢰의 형성 등의 클러스터형성의 주요 요소는 산업형성의 기간이 오래된 성숙지역에 풍부하다고 할 수 있다. 성숙산업지역인 부천의 조립금속산업과 전형적인 혁신지구인 대전의 생물벤처산업의 혁신여건과 혁신출동의 여건분석을 통해, 두 지역의 혁신환경을 비교하고 클러스터의 특성에 따라 차별적인 지역내, 지역간 연계방안, 기업지원체제에 대한 시사점을 도출하였다.

• 한국작물학회지
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• 제49권4호
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• pp.349-353
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• 2004

Mature embryo and leaf base segment of Korean oat were used as materials in an experiment to check plant regeneration efficiency. MS media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, and picloram were used for callus induction from mature embryos and leaf base segments. Three mg/l of 2,4­D and 3 mg/l of picloram in callus induction medium showed high frequency for plant regeneration from mature embryos. Leaf base segments were transferred to callus induction medium and incubated at $25^{\circ}C$ in 16/8 hr light/dark cycle for 3 weeks. Callus induction from leaf base segments of Malgwiri showed high efficiency in medium containing 3 mg/l of 2,4-D and 1 mg/l of kinetin $(91.8\%)$. In case of Samhangwiri, the combinations of phytohormones did not show significant difference. Regeneration from leaf base segments showed high frequency in shoot medium containing 1 mg/l of antiauxin, tri-iodobenzoic acid (TIBA) and 1 mg/l of 6-benzyladenine (BA). Calli induced from leaf base segments of Samhangwiri and Malgwiri in media containing 3 mg/l of 2,4-D and 3 mg/l of picloram showed high regeneration frequency. It appears that the callus initiation medium may be an important factor for subsequent plant regeneration.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.236-243
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• 2009

The halophyte Leymus chinensis (Trin.) is a perennial rhizome grass (tribe Gramineae) that is widely distributed throughout China, Mongolia and Siberia. This study was conducted to investigate an optimal condition for plant regeneration from mature seeds, leaf base segments, and root segments in L. chinensis. Plant growth regulators affecting embryogenic callus induction and plant regeneration were investigated by four-factor-three-level [L9 (34)] orthogonal test in this study. The effects of explants types (mature seeds, leaf base segments and root segments), callus types, medium types were examined in this study. Wild type (WT) and Jisheng No. 1 plants (JS) were used for primary callus induction. A clear explants difference was seen during callus induction; mature seeds were considered as the preferred explants; and the highest frequency of callus induction was obtained in Medium 6 using mature seeds as explants in WT. Plant regeneration ability was evaluated by frequencies of green callus forming, shooting, rooting, and shooting with roots. Effect of α-naphthalene acetic acid (NAA) on shoot regeneration was remarkable with the highest frequency of 70.8% in WT after 2-month culture. The medium with 0.2- 0.5 mg/L NAA was found to have the highest shoot induction. All regenerated shoots were successfully rooted when transferred on half-strength Murashige and Skoog (MS) basal medium. The acclimatized plantlets were grown to mature with flowering and seeds setting in green house conditions.

• International Journal of Oral Biology
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• 제43권1호
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• pp.23-27
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• 2018

Increased intracellular levels of $Ca^{2+}$ are generally thought to negatively regulate lipolysis in mature adipocytes, whereas store-operated $Ca^{2+}$ entry was recently reported to facilitate lipolysis and attenuate lipotoxicity by inducing lipophagy. Transient receptor potential mucolipin1 (TRPML1), a $Ca^{2+}$-permeable non-selective cation channel, is mainly expressed on the lysosomal membrane and plays key roles in lysosomal homeostasis and membrane trafficking. However, the roles of TRPML1 in lipolysis remains unclear. In this study, we examined whether the channel function of TRPML1 induces lipolysis in mature adipocytes. We found that treatment of mature adipocytes with ML-SA1, a specific agonist of TRPML1, solely upregulated extracellular glycerol release, but not to the same extent as isoproterenol. In addition, knockdown of TRPML1 in mature adipocytes significantly reduced autophagic flux, regardless of ML-SA1 treatment. Our findings demonstrate that the channel function of TRPML1 partially contributes to lipid metabolism and autophagic membrane trafficking, suggesting that TRPML1, particularly the channel function of TRPML1, is as therapeutic target molecule for treating obesity.

• 국제학술발표논문집
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• The 4th International Conference on Construction Engineering and Project Management Organized by the University of New South Wales
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• pp.647-652
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• 2011

Developments in the teaching of the National Diploma in Building students should be geared towards meeting the needs of the construction industry in South Africa. These needs are usually in the form of skills and knowledge required to resolve thorny issues encountered in practice. The lack of capacity in the construction industry needs to be addressed from the basis of ensuring that the education system caters for both mature entry and matriculation entry students. The paper looks at a comparison in performance between mature entry and matriculation (just finish High School) entry students focusing on the National Diploma in Building students at the University of Johannesburg. The paper will make a comparison between those students who work first before enrolling for their first year National Diploma in Building and those who are directly from high school. Cooperative education involves training and systematically developing students through the acquisition of the requisite skills, attitudes, values and knowledge required to adequately perform in their chosen careers. The paper describes the problems and experiences that have been encountered by both mature and matriculation entry students in their three years of study which affects their performance. The paper will then look at the value of prior experience in enabling students to finish their National Diploma in Building within a three year period. The paper closes with some recommendations for the future.