• Journal of Mushroom
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• v.21 no.3
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• pp.118-125
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• 2023

We conducted an on-site application study at the livestock cooperative fertilizer plant to compare the composting period, temperature change, moisture content, and chemical properties between livestock manure compost using sawdust as a moisture regulator with those using spent oyster mushroom substrate. The composting period, moisture content, and fertilizer composition of compost containing spent oyster mushroom substrate did not differ from that of conventional compost mixed with sawdust after the first and second fermentation and post-maturation stages, it was suitable as a material for manufacturing livestock manure compost. The spent oyster mushroom substrate also lower the production cost of livestock manure compost by replacing the more expensive sawdust. The developed technology is expected to contribute towards the utilization of by-products of the oyster mushroom harvest while simultaneously producing high quality livestock manure compost.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.4
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• pp.599-607
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• 1998

To clarify annual reproductive cycle of Korean dark sleeper, Odontobutis platycephala (Iwata et Jeon), we examined the seasonal changes of gonadosomatic index (GSI), the proportional frequency of oocyte development stages in the ovary and the changes of sex steroid hormone levels in blood from December 1995 to November 1997. In July and August, GSI was 0.35 to 0.72 and most oocytes in the ovary were chromatin-nucleolus stage and perinucleolar stage (proportional frequency: $87\%\~96\%$). In September, GSI was 1.20 $\pm$ 0.12, some oocytes in the ovary were yolk vesifle stage (proportional frequency: $22.8\%$) and vitellogenic stage which appeared very rarely(proportional frequency: $2.2\%$). GSI increased gradually from October and reached 4.59± 0.61 to December. During this period, oocytes of vitellogenic stage increased slightly (proportional frequency in December: $22.1\%$). In January, GSI was 4.32 $\pm$ 0.72 but the proportional frequency of oocytes in vitellogenic stage increased (proportional frequency: $51.2\%$). from February, GSI was increased sharply and reached to 10.51 $\pm$ 1.04 in March, the highest value throughout the year and the proportional frequency of oocytes in vitellogenic stage also reached the highest levels (proportional frequency: $60\%$). From April, GSI was gradually decreased and fell down to 1.11 $\pm$ 0.35 in June. During this period, the proportional frequency of mature oocytes was the highest in April (proportional frequency of mature oocyte stage: $40\%$ in April, $12\%$ May, $5\%$ June) throughout the year, and atretic ovarian follicles were appeared. The blood level of estradiol-17$\beta$ ($E_2$), which stimulates the hepatic synthesis and secretion of vitellogenin, was $0.84{\pm}0.20\;ng/m{\ell}$ in August, and thereafter was not changed until December. from January, it increased sharply and reached the highest level of $ 2.85{\pm}0.35\;ng/m{\ell}$ in March throughout the year, but fell to $0.14{\pm}0.02\;ng/m{\ell}$ in July(P<0.05), 17$\alpha$-hydroxprogesterone(17$\alpha$-OHP) was the peak $13.37{\pm}0.52ng/m{\ell}$ in March, but no significant changes in other period(below $3ng/m{\ell}$, P<0.05). 17$\alpha$, 20$\beta$-dihydroxy-4-pregnen-3-one(17$\alpha$, 20$\beta$-P), which was known as the final maturation inducing hormone in teleost, was $0.74{\pm}0.09ng/m{\ell}$ in April and $0.54{\pm}0.07ng/m{\ell}$ in May, but no significant changes in other period (below $0.26\;ng/m{\ell}$, p<0.05). Taken together these results, the annual reproductive cycle of O. platycephala divided into 4 periods as follows: 1) ripe and spawning period from April to June, main spawning period was from April to May, 2) Resting period from July to August, 3) Growing period from September to December, 4) Maturing period from January to March. Moreover, It was showed that the changes of sex steroid hormone in blood played a important roles in the annual reproductive cycle of O. platycephala.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.23 no.1
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• pp.27-36
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• 2017

In order to determine the relationship between water content and flower qualities of oriental hybrid lily cv. 'Siberia' cut flower, flowers were subjected to dry and cold storage at $5^{\circ}C$ for 3, 6, and 12 days and subsequently exposed to ambient temperature ($26^{\circ}C$) in bottles with water for up to 16 days. Flowers stored at $22^{\circ}C$ in dry condition for 3 days were used as the control. Changes in fresh weight, moisture content, water balance, flowering stages, osmolality and vase life of cut flowers were observed. Flowers treated with cold and dry storage had higher moisture content compared to control sample. However, this trend was evident only for 3-day cold and dry stored sample during the whole storage period. The fresh weight of cut flowers increased gradually when the samples were transferred to ambient temperature in water bottles and then declined steadily before reaching the peak in between 6-8 days of vase life. However, the changes of fresh weight of control sample were substantially faster than samples pre-treated with cold and dry storage. This was also correlated with the water balance of cut flower as it reached the minus (-) value in 6-8 days of vase life at ambient temperature. Cut lily flowers showed high osmolality values corresponding with the duration of dry storage regardless of low or higher temperature. However, osmolality had no effect on vase life since flower stem absorbed water rapidly at the end of dry storage period. Our vase life results suggest that cold and dry storage of lily cut flowers for a certain period could ensure longer vase life at ambient temperature. It was observed that prolonging the storage period at cold and dry condition for more than a week significantly increased bud abortion, reduced longevity of flowers and reduced the vase life of cut flowers. On the other hand, the shorter cold and dry storage treatment delayed the bud opening and senescence of the flowers, thus, slowering the normal maturation and aging. Results indicated that dry and cold storage at $5^{\circ}C$ for 3 days was effective in maintaining and preserving overall quality and vase life at ambient condition of oriental hybrid lily cut flowers.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.4
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• pp.257-263
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• 2016

Recently, an interest has developed in the use of whole green grains as functional food materials. The present study was conducted to provide the baseline data for the stable production of whole green grains in 20 cultivars of wheat by investigating the greenness of grain with maturation (from $20^{th}$ to $41^{st}$ day after heading, at an interval of 3 days). On the $20^{th}$ day after heading, the grains were dark green with a wrinkled long-oval shape. After the $35^{th}$ day of heading, the grains turned almost yellow with an oval shape. Their redness ('a' value of chromaticity) increased from the $20^{th}$ to $41^{st}$ days after heading, indicating a negative value up to the $32^{nd}$ day after heading. A significant decrease in their chlorophyll content was observed with maturation. The yield of whole green wheat grain (including greenish yellow grain) was the highest from the $32^{nd}$ to $35^{th}$ after heading. Therefore, we concluded that the optimal harvesting period for whole green wheat grain was from the $32^{nd}$ to $35^{th}$ day after heading. The heading time of various cultivars ranged from April 28 to May 5, the time of Jopummil cultivar grew the fastest among them, such as Gurumil, Alchanmil, but Dahongmil got the latest in heading time. The greenness of seven cultivars (Jeokjungmil, Keumkangmil, Jogyeongmil, Jopummil, Baekjungmil, Yeonbaekmil, and Milseongmil) was relatively higher than that of the others. The yield of greenish whole grain was relatively high in six cultivars (Alchanmil, Baekjungmil, Eunpamil, Yeonbaekmil, Dahongmil, and Urimil). Based on their greenness and yield, the Baekjungmil and Yeonbaekmil cultivars have been considered to be optimal for the production of whole green wheat grain.

• Journal of Embryo Transfer
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• v.21 no.1
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• pp.21-27
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• 2006

This study was conducted to determine the distribution of cat follicles among varying ages and produce oocytes from preantral follicles cultured in vitro. We used ovaries from 41 cats ranging in age from 0.3 to 5 years. Ovaries were obtained from cats undergoing routine ovariectomy at local veterinary clinics. As a prelude to in vitro culture of preantral follicles, the length and the width and the weight of ovaries among cats of varying ages were measured. Ovaries were fixed in 10% formalin, embedded in paraffin, cut into $3{\mu}m$-sections, mounted on slides and stained with hematoxylin and eosin. Follicles were evaluated at 200X and 400X magnification. Distribution of follicles among cats of varying ages were evaluated according to follicle classification: primordial, primary, transitional, preantral and antral follicles. Preantral follicles were isolated by the simple mechanical procedure. Each follicle was cultured in a well containing $100{\mu}l$ of medium 199 supplemented with 10% fetal bovine serum (FBS) or polyvinylalcohol (PVA) for 16 days. Follicle diameters were measured under inverted microscope every 4 days. The length, the width and the weight of ovaries were increased gradually according to ages but there was not significant difference among cats of varying ages. Majority of follicles were primordial follicles (84%) regardless of cat ages (p<0.05). Follicle diameter increased until 4 days of culture. However, period longer than 4 days of culture in vitro had a deleterious effect on follicle survival regardless of supplement (FBS or PVA). A few oocytes were collected from preantral follicles cultured in vitro. These basic reproductive techniques in domestic cats can be a useful tool to save endangered feline species.

• The Korean Journal of Food And Nutrition
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• v.23 no.3
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• pp.352-360
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• 2010

This study was performed to develop a Korean natural cheese with traditional medical wine, making it different from foreign natural cheese. The effects of cheese with Sasam(Codonopsis lanceolate) wine(CLW) on the quality properties during the ripening period of natural cheese were investigated. The properties investigated were growth of lactic acid bacteria, characteristics of ripening, and sensory characteristics. Four vats of cheese were made on the same day from the same tank of fresh milk. Cheese samples were prepared with CLW at 2.0%, 4.0% and 6.0% of raw milk. Changes in gross composition, viable cell counts, pH, water soluble nitrogen(WSN), non casein nitrogen(NCN), non protein nitrogen(NPN), and proteolysis during maturation were measured. Polyacrylamide gel electrophoresis(PAGE) patterns were determined with control cheese. Viable cell counts of control and CLW cheese were not significantly different. The pH of CLW cheese increased gradually during maturation, and saponin levels and levels of NPN, NCN, and WSN were higher in CLW cheeses than control cheese. For most compositional data, the 4.0% CLW cheese was most similar to the control cheese. The PAGE pattern of cheese caseins indicated that the CLW cheeses degraded more rapidly than the control cheese. Control and 2.0% CLW cheese had good sensory scores, while scores for 4.0% and 6.0% CLW cheese were lower. However, sensory data depreciated with added levels of CLW, especially at a level of 4.0% or more. Further studies on levels of CLW and processing methods are required to improve sensory quality.

• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.318-331
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• 2021

This study was conducted on the assumption that bone age would be more effective when it comes to physique and physical fitness assessment for adolescents, and the purpose of this study was to identify the differences in physique and physical fitness for students in their adolescence through bone age and chronological age in order to contribute to the well-balanced physique and physical fitness development in adolescents and the health improvement in students. Total 874 adolescents(483 males, 391 females) aged 11~16 were selected as subjects out of the total population of 1100 adolescents aged 6~16 based on the PAPS(Physical Activity Promotion System) and age standards of the TW3 method; and skeletal maturation, which symbolize the indicators of biological maturation, were evaluated by using the TW3(Tanner-Whitehouse 3) method after hand-wrist radiographs, and birth date was used for chronological age. A stadiometer and InBody 270 (Biospace, Korea) were used to measure 2 components in physique. A total of 7 components in physical fitness, which included muscular strength, muscular endurance, flexibility, power, cardiovascular endurance, balance, agility, were measured as well. A independent samples t-test was conducted for data processing using SPSS 25.0, and the significance level was set at p< .05. The study results are as follows. First, bone age and chronological age used for physique comparison in males aged 11 and 12, height and weight showed significant difference; in males aged 13, weight showed signicant difference. Weight and height in females aged 11, and height in females aged 12 showed significant difference. Second, bone age and chronological age used for physical fitness comparison in males aged 11, muscular strength, power, flexibility, cardiovascular endurance showed significant difference; in males aged 12, muscular strength. power, cardiovascular endurance; in males aged 13, flexibility showed significant difference. Muscular strength, power, flexibility, muscular endurance, cardiovascular endurance in females aged 11, and flexibility in females aged 14 showed significant difference. As a result, this study concluded that in a period of rapid skeletal growth, evaluating physique and physical fitness based on bone age is more accurate than evaluating based on chronological age.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.4
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• pp.358-364
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• 2021

This study was conducted to select a desiccant and determine its concentration for safe usage to improve the harvesting efficiency of soybeans. Soybeans were treated with a desiccant (non-selective herbicide) before and after the maturation stage. The drying effect of the desiccant was higher at earlier treatment times than at the maturation stage, but the difference was not statistically significant. The higher efficacy might be related to the drying process of the leaves and stems, with most of the leaves and stems having already been dried by the time of hand harvesting. Desiccant treatments had no adverse effects on soybean yield, weight of 100 grains, seed quality, or seed germination rate compared with the untreated control. Pesticide residue analysis showed minimum residue concentration to be lower than the tolerance level of pesticide residues. In conclusion, it was confirmed that the desiccant was effective in drying soybean, and that there was no damage to the quality of soybean seeds. In addition to the drying effect, the dessicant treatment also facilitates the removal of weeds that interfere with the mechanical harvest and improves harvesting efficiency through the drying of the growth imbalanced individual. The desiccant treatment is expected to shorten the mechanical harvesting time by 1-2 weeks. It is thought that the selection of the proper cultivation period for other crops after soybean cultivation will be more advantageous.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.17
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• pp.115-133
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• 1974

This study was done on the metabolism of chemical components during the seed development of ginseng. The changes of the chemical components were inspected in the following periods: from the early stage of flower organ formation to flowering time, from the early stage of fruiting to maturity, during the moisture stratification before sowing. From flower bud forming stage to meiosis stage, the changes in the fresh weight, dry weight, contents of carbohydrates, and contents of nitrogen compounds were slight while the content of TCA soluble phosphorus and especially the content of organic phosphorus increased markedly. From meiosis stage to microspore stage the fresh and dry weights increase greatly. Also, the total nitrogen content increases in this period. Insolub]e nitrogen was 62-70% of the total nitrogen content; the increase of insoluble nitrogen seems to have resulted form the synthesis of protein. The content of soluble sugar (reducing and non-reducing sugar) increases greatly but there was no observable increase in starch content. In this same period, TCA soluble phosphorus reached the maximum level of 85.4% of the total phosphorus. TCA insoluble phosphorus remained at the minimum content level of 14.6%. After the pollen maturation stage and during the flowering period the dry weight increased markedly and insolub]e nitrogen also increased to the level of 67% of the total nitrogen content. Also in this stage, the organic phosphorus content decreased and was found in lesser amounts than inorganic phosphorus. A rapid increase in the starch content was also observed at this stage. In the first three weeks after fruiting the ginseng fruit grows rapidly. Ninety percent of the fresh weight of ripened ginseng seed is obtained in this period. Also, total nitrogen content increased by seven times. As the fruits ripened, insoluble nitrogen increased from 65% of the total nitrogen to 80% while soluble nitrogen decreased from 35% to 20%. By the beginning of the red-ripening period, the total phosphoric acid content increased by eight times and was at its peak. In this same period, TCA soluble phosphorus was 90% of total phosphorus content and organic phosphorus had increased by 29 times. Lipid-phosphorus, nucleic acid-phosphorus and protein-phosphorus also increased during this stage. The rate of increase in carbohydrates was similar to the rate of increase in fresh weight and it was observed at its highest point three weeks after fruiting. Soluble sugar content was also highest at this time; it begins to decrease after the first three weeks. At the red-ripening stage, soluble sugar content increased again slightly, but never reached its previous level. The level of crude starch increased gradually reaching its height, 2.36% of total dry weight, a week before red-ripening, but compared with the content level of other soluble sugars crude starch content was always low. When the seeds ripened completely, more than 80% of the soluble sugar was non-reducing sugar, indicating that sucrose is the main reserve material of carbohydrates in ginseng seeds. Since endosperm of the ripened ginseng seeds contain more than 60% lipids, lipids can be said to be the most abundant reserve material in ginseng seeds; they are more abundant than carbohydrates, protein, or any other component. During the moisture stratification, ginseng seeds absorb quantities of water. Lipids, protein and starch stored in the seeds become soluble by hydrolysis and the contents of sugar, inorganic phosphorus, phospho-lipid, nucleic acid-phosphorus, protein phosphorus, and soluble nitrogen increase. By sowing time, the middle of November, embryo of the seeds grows to 4.2-4.7mm and the water content of the seeds amounts to 50-60% of the total seed weight. Also, by this time, much budding material has been accumulated. On the other hand, dry stored ginseng seeds undergo some changes. The water content of the seeds decreases to 5% and there is an observable change in the carbohydraes but the content of lipid and nitrogen compounds did not change as much as carbohydrates.

• Journal of Bio-Environment Control
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• v.26 no.3
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• pp.175-180
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• 2017

This study was conducted for the domestic new cultivar to expand cultivation area and sustainable production. The new domestic cultivar, 'Jinok' and control cultivar, 'Campbell Early', had been investigated on vine growth and fruit quality by different cultivating conditions as open field, rain shelter and unheated plastic house. The growth period of 'Jinok' was faster than 'Campbell Early'. The harvesting date of 'Jinok' was on 13th of Aug. faster than 'Campbell Early' which was on 17th of Aug., and it was on 25th and 29th of Aug. for 'Jinok' and 'Campbell Early' on field cultivation, respectively. The growing length and width of new shoots were similar among the cropping systems. In the result of fruit characteristic evaluation, the soluble solids content of the 'Jinok' and 'Campbell Early' cultivating on the unheated plastic house were $15.4^{\circ}Brix$ and $15.9^{\circ}Brix$, respectively. In the open field condition, those were $15.9^{\circ}Brix$ and $15.8^{\circ}Brix$, respectively. The titratable acidity and chromaticity were similar among the treatments. In the appearance of past and disease, Phomopsis blight was occurred on mid of June and in the end of cultivation period on about early Aug., the damage by Phomopsis blight was the lowest about 14% in the unheated plastic house cultivation on the 'Jinok' cultivar and it was 39% in the open field condition. However, in the open filed cultivation for 'Campbell Early', Phomopsis blight was highly occurred about 49%. During this period, or the end of cultivation, it is necessary for the intensive control.