• Title/Summary/Keyword: matK

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Molecular authentication of Lepidii seu Descurainiae Semen by the development of matK amplification primers and analysis of sequences (matK 증폭용 primer 개발 및 염기서열 분석을 통한 정력자(葶藶子) 유전자 감별)

  • Moon, Byeong Cheol;Kim, Wook Jin;Yang, Sungyu;Park, Inkyu;Yeo, Sang Min;Noh, Pureum
    • The Korea Journal of Herbology
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    • v.33 no.3
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    • pp.25-35
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    • 2018
  • Objectives : Lepidii seu Descurainiae Semen has been frequently adulterated with the seeds of several inauthentic plant species. However, the accurate identification of these plant seeds is very difficult. To develop a reliable genetic authentication tool for Lepidii seu Descurainiae Semen, we analyzed matK sequence. Methods : To obtain the matK sequences of plant materials, genomic DNA was extracted from 24 samples and PCR amplification was carried out using matK-AF/matK-8R universal primer set and matK-LDSF/matK-LDSR primer set. For identifying species-specific nucleotides and phylogenetic analysis, matK regions were sequenced and comparatively analyzed by the ClustalW and Maximum Likelihood method. Results : We developed a new primer set to amplify matK region in Lepidii seu Descurainiae Semen and closely related plant samples. From the comparative analysis of matK sequences, we identified species-specific marker nucleotides for D. sophia, L. apetalum, L. latifolium, E. cheiranthoides, E. macilentum, and D. nemorosa, respectively. Furthermore, phylogenetic analysis revealed clear classification depending on the species. These results indicated that the matK sequence obtained a new primer set in this study was useful to identify Lepidii seu Descurainiae Semen in species level. Conclusions : We developed a primer set and identified species-specific marker nucleotides enough to distinguish authentic Lepidii seu Descurainiae Semen and adulterants at the species level based on the matK sequences. These genetic tool will be useful to prevent adulteration and to standardize the quality of Lepidii seu Descurainiae Semen.

A Study on the Growth Characteristics of Riparian Plants on the Artificial Floating Islands (인공섬 수생식물 생육특성에 관한 연구)

  • Kim, Yong-Kyu;Koo, Bon-Hak;Ahn, Tong-Mahn
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.4 no.4
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    • pp.25-35
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    • 2001
  • This study aims to find out the growth characteristics of riparian plants on the artificial floating islands (AFI). Three types of experiments were performed. The vegetation growth were compared by the plant-supporting materials; natural cotton mat, coir mat on the AFI or in the water, and natural soil at the waterfront. Major findings are; 1. The survival rate of riparian plants was 100% on both coir-mat and cotton-matislands, which implies that we may substitute cheaper cotton for expensive coir-mat. 2. Both natural cotton mat and coir mat were good for the growth of plants. Especially, the natural cotton mat was good for Sparganium stoloniferum and Miscanthus sacchariflorus, and the coir mat was good for Typha latifolia. 3. The growth rate of stem and root showed difference between the stem-removed plants and stem-remaining plants. As a whole, the growth rate of stem-removed plants was bigger than the one of stem-remaining plants. 4. The growth rate of plants showed difference between those grown on the AFI and those grown in the water. The growth rate of plant on the AFI was bigger than the one in the water. 5. The growth rate of plants on the AFI was bigger than the one on the natural soil at the waterfront.

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Improved Electrical Conductivity of a Carbon Nanotube Mat Composite Prepared by In-Situ Polymerization and Compression Molding with Compression Pressure

  • Noh, Ye Ji;Kim, Han Sang;Kim, Seong Yun
    • Carbon letters
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    • v.13 no.4
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    • pp.243-247
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    • 2012
  • A fabrication method to improve the processability of thermoplastic carbon nanotube (CNT) mat composites was investigated by using in-situ polymerizable and low viscous cyclic butylene terephthalate oligomers. The electrical conductivity of the CNT mat composites strongly depended on the compression pressure, and the trend can be explained in terms of two cases, low and high compression pressure, respectively. High CNT mat content in the CNT mat composites and the surface of the CNT mat composites with fully contacted CNTs was achieved under high compression pressure, and direct contact between four probes and the surface of the CNT mat composites with fully contacted CNTs gave resistance of $2.1{\Omega}$. In this study the maximum electrical conductivity of the CNT mat composites, obtained under a maximum applied compression pressure of 27 MPa, was 11 904 S $m^{-1}$, where the weight fraction of the CNT mat was 36.5%.

Analysis of the MAT1-1 and MAT1-2 Gene Ratio in Black Koji Molds Isolated from Meju

  • Mageswari, Anbazhagan;Kim, Jeong-seon;Cheon, Kyu-Ho;Kwon, Soon-Wo;Yamada, Osamu;Hong, Seung-Beom
    • Mycobiology
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    • v.44 no.4
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    • pp.269-276
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    • 2016
  • Aspergillus luchuensis is known as an industrially important fungal species used for making fermented foods such as awamori and shochu in Japan, makgeolli and Meju in Korea, and Pu-erh tea in China. Nonetheless, this species has not yet been widely studied regarding mating-type genes. In this study, we examined the MAT1-1 and MAT1-2 gene ratio in black koji molds (A. luchuensis, Aspergillus niger, and Aspergillus tubingensis) and in Aspergillus welwitschiae isolated from Meju, a fermented soybean starting material for traditional soy sauce and soybean paste in Korea. The number of strains with the MAT1-1 locus was 2 of 23 (A. luchuensis), 6 of 13 (A. tubingensis), 21 of 28 (A. niger), and 5 of 10 (A. welwitschiae). Fungal species A. tubingensis and A. welwitschiae showed a 1 : 1 ratio of MAT1-1 and MAT1-2 mating-type loci. In contrast, A. luchuensis revealed predominance of MAT1-2 (91.3%) and A. niger of MAT1-1 (75%). We isolated and identified 2 A. luchuensis MAT1-1 strains from Meju, although all strains for making shochu in Japan are of the MAT1-2 type. These strains may be a good resource for breeding of A. luchuensis to be used in the Asian fermented-food industry.

Experimental Study on Characteristics of Natural Fiber Mat (수평 천연섬유배수재의 공학적 특성 평가를 위한 실험적 연구)

  • Kim, Ju Hyong;Cho, Sam-Deok
    • Journal of the Korean Geosynthetics Society
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    • v.4 no.2
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    • pp.3-9
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    • 2005
  • Natural fiber mat made with compressed coconut mat and jute filter is in the spotlight recently as an alternative material for sand mat, which is getting expensive in Korea. Tensile strength and permeability tests for the natural fiber mat were carried out to evaluate for its practical use in this study. Despite of very low tensile strength of coconut mat, that of jute filter was satisfied with conventional criteria of geotextile suggested by Christopher and Holtz(1985). Besides, permeability of fiber mat under high compressive pressure was greater than that of conventional sand material used as sand mat. It was found that the fiber mat has a great potential in substituting the conventional sand material.

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Sheetlike Waveguide for 2.4 GHz and 5 GHz Bands

  • Eom, Kun-Sun;Hiroyuki, Arai
    • ETRI Journal
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    • v.32 no.1
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    • pp.112-119
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    • 2010
  • We present a useful design for a free access mat which supports two frequency bands of 2.4 GHz and 5 GHz. The free access mat is a sheet-shaped waveguide which consists of a tightly coupled double-layered microstrip resonator array. It provides easy access for devices in short-range wireless communications. Interference is a common problem with conventional applications which use free space transmission. Our proposed wireless access system uses a subsidiary waveguide, the free access mat. Wireless devices are proximately coupled to the free access mat through which the coupled electromagnetic (EM) wave transmits. The arrival domain of the EM wave of an application is therefore limited to an area close to the free access mat. Wireless devices can be coupled to the free access mat at an arbitrary position without contact. We previously presented a free access mat for a single frequency band. This paper presents a free access mat for the two frequency bands of 2.4 GHz and 5 GHz. The free access mat uses a ring patch resonator array which is easily excited by typical antennas and is resistant to interference. These characteristics are demonstrated by numerical simulation and confirmed by experiment.

Control of Turbid Water Transport with Filamentous Mat (섬모상 매트에 의한 탁수이동차단에 관한 실험적 연구)

  • Yu, Jianghua;Yi, Qitao;Kim, Youngchul
    • Journal of Korean Society on Water Environment
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    • v.26 no.1
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    • pp.44-51
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    • 2010
  • A lab-scale apparatus for turbid water transport control was tested and examined. The channel had a dimension of $100cm{\times}30cm{\times}15cm$ (length${\times}$hight${\times}$width). And the turbidity water was prepared using two types of particles, bentonite and loess. The channel equipped with filamentous mat was operated under various shock load conditions. In the control channel, instantly, turbid water mixed with the clean water inside the channel and turbidity prevails the entire channel. While in the mat-equipped channel, it increases only at the bottom. Overall, the filamentous mat gave capture efficiency of 70~90% compared with the control group. The capture efficiency of turbid particles decreased with increased input turbidity flux. The result of experimental run on how turbid particles are separated in the mat channel shows that settling, filtration and attachment are the main processes. Meanwhile, turbidity was diffused from the channel bottom due to turbidity gradient before and after mat zone. The particle size before mat zone was lightly coarser than that after mat zone.