• Title/Summary/Keyword: mass propagation system

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Developing a mass propagation technique for Aralia elata via somatic embryogenesis

  • Moon, H.K.;Lee, J.S.;Kim, T.S.
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2000.10b
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    • pp.16-17
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    • 2000
  • Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will hel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/ or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field will be presented. And some problems arising for the somatic embryogenesis system will be also discussed.lso discussed.

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Dormancy-breaking and Germination of Chelidonium majus L. subsp. asiaticum H. Hara Seeds by Stratification and Gibberellins

  • Boran Ji;Hayan Lee;Kyungtae Park;Sang Yeob Lee;Bo-Kook Jang;In Hwan Chae;Chung Youl Park;Sung Pil Kwon;Deug-Chan Lee;Ju-Sung Cho
    • Korean Journal of Plant Resources
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    • v.35 no.6
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    • pp.796-804
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    • 2022
  • The demand for Chelidonium majus L. subsp. asiaticum H. Hara is expected to increase due to its pharmacological properties such as antibacterial, antioxidant, and anti-inflammatory effects. However, an effective propagation system for this species has not yet been established. This study was conducted to analyze the seed dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara native to Korea and establish a mass propagation system. The dormancy type was primarily classified by analyzing the general information of the collected seeds. The seed dormancy breaking was investigated by comparing the effects of cold stratification (0, 2, 4, 8, 10, or 12 weeks) with warm stratification (S, summer temperature, 25/15℃) and intermediate temperature stratification (A, autumn temperature, 15/10℃) of alternating temperature stratification (S12-A4 or S12-A8 weeks). After dormancy break, 500 mg/L GA3 and GA4+7 treatment replaced cold stratification and improved seed germination. The results of this study are expected to provide basic data for future seed propagation and mass propagation by analyzing the dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara seeds.

Proposal of Virtual Sensor Technique for Quadruped Robot using Backpropagation Neural Network (Back propagation 신경망이론을 이용한 4 족 보행로봇의 가상 센서 기술 제안)

  • Kim, Wan-Soo;Yu, Seung-Nam;Han, Chang-Soo
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.894-899
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    • 2008
  • Measured sensor datum from a quadruped robotics is commonly used for recognizing physical environment information which controls the posture of robotics. We can advance the ambulation with this sensed information and need to synthesize various sensors for obtaining accurate data, but most of these sensors are expensive and require excessive load for the operation. Those defects can be serious problem when it comes to the prototype's practicality and mass production, and maintenance of the system. This paper suggests virtual sensor technology for avoiding previous defects and presents ways to apply a theory to a walking robotics through virtual sensor information which is trained with several kinds of actual sensor information from the prototype system; the general algorithm is initially based on the neural network theory of back propagation. In specific, we verified a possibility of replacing the virtual sensor with the actual one through a reaction force measurement experiment.

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Efficient Propagation by Bioreactor System of Korean Native Seosanjong in Ginger

  • Jo, Man-Hyun;Ham, In-Ki;Lee, Mi-Ae;Lee, Eun-Mo;Song, Nam-Hyun;Woo, In-Shik
    • Plant Resources
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    • v.3 no.3
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    • pp.219-222
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    • 2000
  • For the purpose of establishing an efficient propagation through airlift bioreactor system of Zingiber of officinale Rosc. Korean native Seosanjong, the effect of different factors and bioreactor on cultured plantlets were investigated. The highest number of plantlets, fresh weight per plant was obtained from explants when cultured in MS liquid medium including 0.3 mg/L NAA and 2.0 mg/L kinetin for 40 days. A 10 L bottle type bubble bioreactor, compared with 250 mL Erlenmeyer flask, was more efficient, producing 4.7 plantlets or from 1.5 to 1.6 times more than did the Erlenmeyer flask. The results demonstrate the rapid mass propagation of airlift bioreactor to produce normal ginger.

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Response of orthotropic Kelvin modeling for single-walled carbon nanotubes: Frequency analysis

  • Hussain, Muzamal;Naeem, Muhammad N.;Tounsi, Abdelouahed
    • Advances in nano research
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    • v.8 no.3
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    • pp.229-244
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    • 2020
  • In this paper, modified Kelvin's model has been used to analyze the orthotropic vibration frequencies of single walled carbon nanotubes with clamped-clamped and clamped-free boundary conditions. For this system the governing equation is developed with wave propagation approach. Armchair, zigzag and chiral structures are considered for the vibrational analysis to investigate the effect of different modes, in-plane rigidity and mass density per unit lateral area. Throughout the computations, on decreasing the length-to-diameter ratios, the frequencies of said structure increases. In addition, by increasing three different value of in-plane rigidity resulting frequencies also increase and frequencies decrease on increasing mass density per unit lateral area. The results generated using computer software MATLAB to furnish the evidence regarding applicability of present model and also verified by available published literature.

An Experimental and Mathematical Study on the Effects of Ignition Energy and System on the Flame Kernel Development

  • Song, Jeonghoon;Sunwoo, Myoungho
    • Journal of Mechanical Science and Technology
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    • v.16 no.6
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    • pp.829-838
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    • 2002
  • A constant volume combustion chamber is used to investigate the flame kernel development of gasoline air mixtures under various ignition systems, ignition energies and spark plugs. Three kinds of ignition systems are designed and assembled, and the ignition energy is controlled by the variation of the dwell time. Several kinds of spark plugs are also tested. The velocity of flame propagation is measured by a laser deflection method, and the combustion pressure is analyzed by the heat release rate and the mass fraction burnt. The results represent that as the ignition energy is increased by enlarging either dwell time or spark plug gap, the heat release rate and the mass fraction burnt are increased. The electrodes materials and shapes influence the flame kernel development by changing he transfer efficiency of electrical energy to chemical energy. The diameter of electrodes also influences the heat release rate and the burnt mass fraction.

In-cylinder Flame Visualization and Flame Propagation Characteristics of SI Engine by using Optimal Threshold Method (Optimal Threshold 법을 이용한 가솔린 기관의 실린더 내화염 가시화 및 화염 전파 특성에 관한 연구)

  • 김진수;전문수;윤정의
    • Transactions of the Korean Society of Automotive Engineers
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    • v.8 no.5
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    • pp.96-104
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    • 2000
  • It is well known that combustion stability under idle and part-load conditions directly affect fuel economy and exhaust emission. In practice, there have been a lot of studies so that a significant improvement in combustion stability has been achieved in this research field. However, applying published results to the development process of mass production engine, there are still many problems which are solved previously. In this study, initial flame behavior and flame propagation characteristic were investigated statistically in order to optimize combustion chamber shapes in the development stage of mass production S.I. engine. To the purpose, the authors applied the flame image capturing system to single cylinder optical engine. The captured flame images were effectively analyzed by using the image processing program which was developed by the authors and adopted new threshold algorithm instead of conventional histogram analysis. In addition, the cylinder pressure was also measured simultaneously to compare evaluated flame results with cylinder pressure data in terms of the combustion characteristics, combustion stability, and cycle-to-cycle combustion variability.

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Establishment of propagation system for in vitro calla plants (Zantedeschia spp.) by treatment of taurine (타우린 처리를 통한 칼라 기내 식물체 대량증식체계 확립)

  • Lee, Sang Hee;Kim, Young Jin;Yang, Hwan Rae;Kim, Jong Bo
    • The Journal of the Convergence on Culture Technology
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    • v.4 no.4
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    • pp.331-335
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    • 2018
  • Zantedeschia spp. calla is very popular as a cut flower. It is very important to establish a micro propagation system through plant tissue culture with the problem that colored calla with various colors are low in natural reproduction rate and vulnerable to high temperature. In this study, we conducted the experiment by adding taurine to improve the growth of calla plant. When 20 mg/L of taurine was added with plant growth effect, 54.0 % of the cases of multiple shoots and 17.2 times of fresh weight were the most effective. Taurine 20 mg/L treatment showed 16.0 % and 39.2 %, respectively, than the untreated control. Taurine may contribute to mass propagation of elite breeding lines as well as an improvement of farm income by positively influencing the overall growth of calla plants, thereby positively affecting the establishment of the micro propagation system of calla shoot tips.

Study on the Propagation System and the Photosynthetic Rate of Chrysantemum zawadskii H. (약용자원식물 구절초의 고소득화를 위한 번식체계 확립 및 재분화 식물체의 광합성 능력증대 I. 구절초의 기내배양 및 재분화 식물체의 RAPD 분석)

  • 김정률
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.1-8
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    • 1998
  • This study was conducted to establish mass propagation system from the axillary bud culture of chrysanthemum zawadskii H. which was used as material of medicinal plants. Shoot egeneration was better on MS medium with NAA and BA. The optimum concentraions of growth regulator for shoot regeneration differed depending on accessionsof C. Zawadskii. Shoot regeneration in Keungucheolcho was better on MS Medium with NAA 0.01mg/1 and BA 0.1mg/1 while Hyangrobonggucheocho was better with NAA 0.1mg/1and BA 0.3mg/1. Addition of NAA into medium was effective for induction of root from shoots regenerated. Shoot multiplcation was more effective when 10mg/1 spermine was added into medium than when other polyamines were treated ino medium . Randomly and specifically amplified polymorphic DAC banding patterns based on polymerase chain reaction (PCR) analysis were used to assess the genetic variation of plants regenerated from in vitro culture.

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A Wave Propagation Analysis in the Layered Systems (적층계(積層係)를 통과하는 소성응력파(塑性應力波)의 전파(傳波))

  • Lee, Sang Ho;Ahn, Byoung Ki;Kang, Young Goo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.13 no.2
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    • pp.61-71
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    • 1993
  • The stress waves generated by the mechanical energies by impact or the chemical energies by the explosions are transmitted through medium. The wave propagation process through medium is a very complicated procedure due to the reflections and refractions of the waves at the free surfaces and interfaces. In this study the pressure independent Von-Mises model is employed for the wave propagation analysis in the layered systems. Governing equations of this study are conservation equations of momentum and mass in Lagrangian coordinate system which is fixed to the material. Due to the shock-front which violates the continuity assumptions inherent in the differential equations numerical artificial viscosity is used to spread the shock front over several computational zones. These equations are solved by Finite Difference Method with discretized time and space coordinates. The associate normality flow rule as a plastic theory is implemented to find the plastic strains.

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